• Korean Journal of Environmental Biology
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• v.21 no.2
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• pp.181-188
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• 2003

In this study, soil seed and bud bank analysis were performed to abbess the mass of potential vegetation in soils less than 1 year old after covered and estimate the amount of seed bank transport through construction in urban area. The ratio of exotic species number to total species number in study sites and the landfill control site was 0.29 and 0.39, respectively. All plots pooled, mean species number and total mean seedling density per $\m^2$ in the top 10 cm of soil was 11$\pm$0 (average$\pm$S.E.) and 8037$\pm$221.Total plant seeds by artificial soil trasnsfer were estimated to be 53 thousand million $m^{-2}yr^{-1}$ in 10 cm soil depth. It reveals that soil transfer accelerates seeds and vegetation movement and makes urban vegetation mixed and common.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.15-16
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• 2003

• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.497-506
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• 2018

The purpose of this study was to investigate the proper minimum viable population of a seed bank for the ecological restoration of pine forest using a seed bank. It examined the germinated soil seed bank from August 2010 to November 2011. The results of the correlation analysis showed that the area and the number of herbaceous species were the highest at 0.686. The results of the regression analysis of four variables including the number of woody species, the number of woody individuals, the number of herbaceous species, and the number of herbaceous individuals using the theory of island biogeography to the minimum viable population in studied plots showed that all four variables were significant with area at the level of 0.05, and R square was 0.583. One function was selected between the number of species and the number of individuals from the canonical correlation analysis, and the function square was 0.824. Both canonical function and squared canonical correlation showed significant at the level of 0.01. The result of study recommended the area size of the minimum viable population in pine forest applied by seed bank to be larger than $64m^2$, the number of species to be over 21, and the number of population to be over 120. It also found that the number of herbaceous species determined the cluster size of the seed bank. Therefore, it is necessary to consider herbaceous species that appear in the seed bank.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.4
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• pp.46-56
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• 2008

This study examined the natural restoration of vegetation through monitoring of the development of a vegetation community from 2006 through 2007 after a forest fire. Approximately 5,000 $m^2$ in a forest near Topyeon-ri, Kangnae-myeon, Chungcheongbuk-do with Japanese red pine (Pinus densiflora) forest and its floor vegetation had been completely burned by a fire in April 2005. This area and another nearby Japanese red pine forest were selected as the experiment site and the control site, respectively. Vegetation survey was conducted at the experiment site and the control site. A seed bank experiment was carried out in the greenhouse to examine underground vegetation. Effective microorganism(EM) was applied to the seed bank experiment to estimate its effects on the direction of ecological succession. According to the results, a total of 36 plant species including shrub and herbaceous species were discovered in the experiment site. Quercus serrata, Lespedeza cyrtobotrya, and Castanea crenata, Rubus crataegifolius, Oplismenus undulatifolius, and Carex lanceolata were among the most abundant species. Biomass in the experiment site reached 2.4 times biomass than those in the control site, indicating the productivities of shrub and herbaceous layers are better in the experiment site. According to the result of the soil seed bank experiment of the experiment site, a total of 182 plants of 14 species were recorded. In addition, a total of 13 plants of 2 species were found from soil seed bank of the experiment site applied by EM. If EM is applied to the burned site, it will control the budding of herbaceous plants, creating the gap between herbaceous plants. This loss of competition is expected to help the restoration of trees in the burned area.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.144.2-145
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• 2003

This study was performed to investigate the incidence of Hosta uirus X (HVX), a Potexvirus, from cultivated hosta ornamental plants in Korea and to ascertain seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants. Infection rate of HVX in cultivated hostas was 25.6 ％ (11 out of 43 collected samples contained HVX) based on Western blot and RT-PCR detection methods. Most of HVX-infected hostas showed visible systemic leaf symptoms (mosaic, mottle, curling, stunting or combinations). Variability of HVX was confirmed by sequences of coat protein gene of individual isolates from different hostas. HVX was seed-transmitted on Hosta 'Blue Cadet'. The virus was detected from seeds, and sprouts and seedlings from the virus-contaminated seed sources. Over 7.5 ％ of seeds were HVX-contaminated surveyed in this study, Our data suggest that HVX can be transmitted by seed source, and indexing of the virus should be done for breeding program of Hosta.

• Journal of Forest and Environmental Science
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• v.38 no.3
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• pp.141-151
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• 2022

Soil seed banks as natural storage of plant seeds play an important role in the maintenance and regeneration of watershed. Natural regeneration potential of the soil seed bank of Land use types (LUTs) in Ogun River watershed (ORW) was investigated. ORW was stratified using proportionate sampling technique into Guinea Savannah (GS), Rainforest (RF) and Swamp Forest (SF) Ecological Zones (EZs). Three LUTs: Natural Forest (NF), Disturbed Forest (DF) and Farmland (FL) were purposively selected in GS: GSNF, GSDF, GSFL; RF: RFNF, RFDF, RFFL and SF: SFNF, SFDF, SFFL, respectively. Systematic line transects was used in the laying of the sample plots. Sample plots of 25 m×25 m were established in alternate positions. Ten 1 m×1 m quadrats were randomly laid for soil core sampling from previously randomly selected ten plots. The core samples (10) were pooled per plot in each LUT and placed in individual trays. Ten trays with sterilized soil were used as control. The trays were watered regularly and checked for seedlings emergence fortnightly for 18 months. The experimental design used was 3×3 factorial experiments. ANOVA, Diversity index (H') and Similarity index (SI) were used to analyze the data. There was significant difference in seedling emergence among ecological zones and land use types (p<0.05). A total of 4,400 seedlings emerged from the soil samples. All species were distributed among 32 families. FL in the RF had the highest number of germinated seeds (705±37.33 seedlings) followed by DF in the RF (701±49.6 seedlings). The lowest emergence was in NF of the SF (199±28.41 seedlings). DF in the RF had highest number of species (34) distributed among 22 families. Emergence from soil seed bank of NF in ORW was generally with more of tree species than herbs that were predominant in FL and DF.

• Korean Journal of Environment and Ecology
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• v.24 no.6
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• pp.638-647
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• 2010

This study was performed in order to analyze influences the actual vegetation between the communities of Pinus densiflora and Quercus mongolica in southern Korea to the species composition of the seed bank in the top soil. The soil samples were collected from the studied plots and transported to the Experimental Glasshouse at Department of Landscape Architecture, Yeungnam University and examined the germinated species individual numbers with species composition and germinated individual numbers by species. The overall germination status was the highest in the herbaceous layers than the layers of tree, sub-tree and shrub, respectively. There were no significantly different between numbers of species and individuals and between actual vegetation and seed bank in the two communities. The woody species marked in the seed bank as a pioneer or edge species than in the two communities which are in the stage of mid or late successional species. Although the plant species in the herbaceous layers was highly influenced to the species composition of the seed bank, but no significantly different was showed within the same forest zone and latitudes of Korean peninsula. As the pioneer or edge species of the actual vegetation possibly will be higher the buried in the top soil layer and this phenomenon will influence overall species composition in the seed bank population.

• Journal of Environmental Science International
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• v.27 no.10
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• pp.907-923
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• 2018

The aim of this study was to determine the appropriate stockpiling methods for revegetation by comparing the germination status of seed banks before and after preservation for 2 years. Soil temperature in stockpiled topsoil was higher in open treatment and at 1.5 m, whereas soil water content was maintained at lower levels (14.06-19.08%), than those in the control group. The seed banks in stockpiled topsoil had 48 species and 1,559 individuals, among which perennials showed the highest number in terms of life forms, whereas Compositae and Gramineae were dominant in terms of families. Based on seed bank type, persistent seed banks had the highest number of species, while transient seed banks had the highest number of individuals. By stockpiling period, the number of species in the seed bank started to increase after 24 months, while the number of individuals began increasing after 12 months and exceeded that of the control group after 24 months. Regarding the treatment of stockpiling methods, the number of species and individuals in open treatment were closer to those of the control group. When analyzed by height, the number of species and individuals were higher at 0 m, but still lower than those of the control group. A multivariate analysis of variance (MANOVA ) showed that the optimal combination was obtained in open treatment and the number of individuals increased with the lengthening of the stockpiling period.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.53-61
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• 1999

This study was conducted to determine the optimal collecting time and methods of utilization of forest topsoil as a seed-bank source for revegetation materials of slopes. From April to November of 1998, nursery seedbeds in the greenhouse of Seoul National University were seeded with mixtures of forest topsoil and seeds. The nursery seedbeds were treated to evaluate the effect of depths of forest topsoil and seeding amounts. Seasonal experiments with $3{\times}3$ factorial design were conducted in April, June and August. More than 40 seedlings/$m^2$ were observed as the naturally-emerged species in the plot, treated with the top-layer forest soil within the depth of 10cm. However, it was found, as seeding more seeds, the number of appearance of the naturally-emerged species reduced because of the heavy competition between the seeded and the naturally-emerged. According to the results of this research, it is recommended that seeding amount should be no more than 1,000 seeds/$m^2$ to avoid the severe competition. Also it was observed that the forest topsoil collected in spring(April) is better than that collected in summer. The more species of the naturally-emerged were found in the forest topsoil collected in spring. Forest-topsoil-mixed-materials as a seed-bank source could significantly increase the plant diversity and productivity. Effective use of forest topsoil as a seed-bank source may become a valuable tool in future restoration of disturbed slopes for promoting plant community diversity and recycling of spoiled-soils from the slopes.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.302-307
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• 2004

Three pairs of specific primers were developed for rapid and precise RT-PCR detection of three seed-transmissible viruses, namely Peanut clump virus (PCV, Pecluvirus), White clover mosaic virus (WCIMV, Potexvirus) and Carrot red leaf virus (CaRLV, Luteovirus). Each primer set was found in conserved region through multiple sequence alignment in the DNAMAN. Total nucleic acids extracted from PCV-, WCMV-, and CaRLV-infected seeds and healthy plants were used for RT-PCR detection using each virus-specific primer, Sizes of PCV, WCIMV, and CaRLV PCR products were 617bp (PCV-uni5 and PCV-uni3 primers), 561bp (WCMV-CP5 and WCMV-CP3 primers), and 626bp (CL1-UP and CL2-DN primers); which corresponded to the target sizes. Nucleotides sequences of each amplified cDNA were confirmed which belonged to the original virus. This study suggests that these virus-specific primer sets can specifically amplify viral sequences in infected seeds. Thus, they can be used for specific detection of three viruses (PCV, WCMV and CaRLV) from imported seed samples for plant quarantine service.