• The Korea Journal of Herbology
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• v.34 no.3
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• pp.19-30
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• 2019

Objectives : This study is to propose the identification keys based on stereoscopic examination of 8 seed herbs in 2 categories (Phyllolobobii Semen (PS) with 4 Astragali Semen (AS), and 2 Lepidii seu Descurainiae Semen (LDS) with 1 Drabae Semen (DS)) which have difficulties in discrimination with visual observation. Methods : We reviewed the description of original plants and their medicinal parts from the literature. The original plants were collected, identified, confirmed as specimens, and compared to the samples distributed in the market. The first identification was made by visual observation, and insufficient points were supplemented by stereoscopic observation. Identification criteria were set by considering morphological characteristics of authentic herbs, percentage of adulterants, and distinction between authentic herbs and adulterants. Results : The original plants of PS and AS could be distinguished by upright or lying form of stem, color of flowers, number of leaflets, and presence of hair of fruits. LDS and DS could be distinguished by leaf arrangement on stem: radical or cauline, whole plants size, leaf division, color of flowers, and shape of fruits. The herbal medicines of PS and AS could be distinguished by seed surface pattern, size, and hardness. LDS and DS could be distinguished by size, shape, viscosity when chewed, and degree of mucous layer formation when soaked in water. Conclusions : This study suggests the identification keys of original plants and herbal medicines. Especially, since fine seed herbs are difficult to distinguish by visual observation, the stereoscope should be applied to the discrimination.

• Journal of Radiation Industry
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• v.8 no.2
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• pp.111-121
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• 2014

This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leaf mustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated with gamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration to enlarge the characteristic morphological variations were also separately investigated. The survival rates of S-28 only linearly decreased with increasing the gamma-ray dose. The overall growth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. The reduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those of S-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 and S-27 showed no significant differences by EMS treatment, while the growth of all three lines were significantly decreased. The reduction concentration 50 in S-14 could not be determined, demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, while those in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseed lines possess higher sensitivity to EMS than S-14. Various morphological characteristics of $M_1$ generation obtained from mutagen treatment were elaborately investigated for further maintenance of $M_2$ generation. In $M_2$ generation variants showing short stem, yellow color in seed coat, chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially useful mutants for breeding.

• Korean Journal of Plant Resources
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• v.25 no.4
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• pp.401-406
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• 2012

This study was conducted to investigate the effect of cone protective net and the variation of cone and seed characteristics of a unique and rare dwarf stone pine (Pinus pumila Regel) population in Mt. Seorak which is in the southern peripheral of South Korea. Iron protective net (50 mesh, 25 ${\times}$ 25 cm) was effective in reducing birds and rodents damage to conelet, thereby it was possible to safely collect seed genetic resources. Early July was observed to be the most appropriate season to cover conelet with Iron protective net. P. pumila have ovoid-triangle shaped, wingless seeds and indehiscent cones, seeds dissemination is mainly by the hoarding behavior of nutcrackers and rodents. Statistical analysis showed that there were significant differences among individuals within population, and the mean characteristics of the species were 35.3 mm, 25.6 mm, 1.38, 6.6 g and 39.3 ea in the cone length, cone width, cone index, cone weight and in number of seeds per cone, respectively. Coefficients of variations in seed weight and number of seeds per cone were relatively high (21.7%, 21.5%, respectively) compared to other traits. Based on the correlation analysis between cone characteristics and seed characteristics, the length of cone and seed showed a significant positive correlation with the seed size and the seed weight, but the width and thickness of seed showed a negative correlation with the number of seeds per cone.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.351-354
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• 1998

Centipedegrass (Eremochloa ophiuroides) is a popular lawn grass in the southeastern USA. It has a naturally light green color and grows well on a wide range of soil types. Studies show limited morphological variation present in centipedegrass germplasm. To obtain the high morphological variation, plants were established from the irradiated seed at 10 Kr, allowed to interpollinate and harvested bulk seed, and then irradiated again for the next cycles. Morphological characteristics were measured in the 5 genetic varition lines (TC201 : cv. Common and non irradiated, TC202 : 4th cycles, TC241 : 6th cycles, TC306 : 8th cycles, and TC318 : 5th cycles) induced by recurrent gamma radiation. The ranges of variation of recurrently radiated centipedegrass lines < TC202, TC241 and TC306 except TC318(TifBlair) > for the stolons per plant, total stolon length per plant, longest stolon length, leaf length and width at top-most exposed internode were wider than those of non-irradiated line (TC201). Recurrent gamma radiation was very effective to enlarge the ranges of variation of morphological characteristics in reproductive organ like stolons of centipedegrass. The effect of quantity of gamma ray irradiation cycles on the means and ranges of variation in the morphological characteristics of centipedegrass was not regularly tended.

• Journal of Korean Society of Forest Science
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• v.79 no.4
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• pp.424-430
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• 1990

The variation of needle, cone and seed characteristics and of allelic frequency in isoenzyme, ADH, ME and PGI, according to different resin duct index in Pinus densiflora, Pinus thunbergii and their hybrids was analyzed. The results obtained were as follows : 1. With increase of number of resin ducts, morphological characteristics such as needle length, needle sheath length, cone size, seed size, seed wing size, 1000 seeds weight, etc. tended to he increased, while number of stomata row in needle to be decreased. 2. As the results of discriminant analysis for the morphological characteristics of needle, cone and seed, most individuals are generally coincided with number of resin duct mostly in Pinus densiflora were not. 3. According to the canonical discriminant function obtained from the morphological characteristics in Pinus densiflora, Pinus thunbergii and their hybrids including introgressive hybrid, the resin duct index, 1000 seeds weight, cone size and neelde sheath length characterized fairly their species. 4. With increase of resin duct index, hybrid index tended to be higher. The results obtained from the discriminant analysis and the hybrid index were nearly same each other. 5. With increase of number of resin duct, the allelic frequencies for isoenzyme, ADH-$B_2$, ME-$A_2$ and PGI-$B_1$. $B_2$ tended to increase but those of ADH-$B_3$, ME-$A_4$, and PGI-$B_3$, to decrease. It is thought that this increase of frequency for the former four isoenzymes was resulted in introgressive gene flow from Pinus thunbergii to Pines densiflora and accordingly the frequency of latter three isoenzymes tended to decrease.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.293-302
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• 2020

Morphological characteristics of Ziziphus jujuba accessions, including 'Ilbon', were investigated to verify the possibility of 'Ilbon' as a breeding material. 'Ilbon' has smaller leaves and fruits and a lower total soluble solids content compared to the major Z. jujuba accessions. However, total polyphenol content and antioxidant capacity were highest in 'Ilbon' among the Z. jujuba accessions. In addition, 'Ilbon' could produce intact seeds and seed productivity of 'Ilbon' was highest compared with major Z. jujuba accessions. Principal component analysis and clustering analysis using the morphological characteristics of Z. jujuba accessions have been classified them to geographical origin. In particular, 'Ilbon' was clearly distinguished from the major Z. jujuba accessions. Therefore, 'Ilbon', which has the highest seed productivity and functional component, will be used as a material for cross breeding and will promote genetic study of Z. jujuba.

• Natural Product Sciences
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• v.8 no.3
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• pp.83-89
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• 2002

Butea monosperma Kuntze, commonly known as 'Palash', is employed in various indigenous systems of medicine against several diseases and almost every part of the plant has diversified medicinal properties. The seeds are used as anthelmintic, aperient, digesti and to treat piles, skin diseases and abdominal troubles. They also have the property of reducing 'Kapha' and 'Vata' (in Ayurveda). In the present paper a detailed pharmacognostical evaluation of seeds has been undertaken. The study includes macro- and micro-scopical details, fluorescence powder study and HPTLC fingerprinting. The seed is characterized by finely ridged seed coat and palisade-like malpighian cells, discontinuous transparent linea lucida in upper half of malpighian layer and simple & oblong hilum. The study also concludes that the seed samples procured from different places have similar morphological and physico-chemical characteristics. These observations are also supported by similar TLC profiles. The estimation of heavy metals (to detect permissible toxic limits), and fatty acid composition have been carried out. An attempt has also been made to see the ecological and edaphic variations, if any.

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.1-8
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• 2013

This study was conducted to examine the relationship between cone abundance and cone/seed characteristics to improve seed quality and seed orchard management of Pinus koraiensis. The data was collected from P. koraiensis seed orchards at Chungju in 2007. That year was a rich year of cone harvest and the average number of cone per tree was 2.5 times much than that of normal year. We collected the cones from all trees in the sample plot of the seed orchard and investigated morphological characters such as cone length, cone width and cone weight. According to cone abundance per tree, the samples were divided into eight classes and analysed by cone analysis method. There is a decreasing tendency of number of fertile scale, seed potential, percent of filled seeds, seed weight per cone and seed efficiency, when the cone number per tree is increased. Additionally, the implications of the results to seed orchards management of P. koraiensis were discussed.

• Korean Journal of Plant Resources
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• v.24 no.1
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• pp.69-75
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• 2011

This study was conducted to investigate the variation of cone, seed and germination characteristics among populations and among individuals within populations of Picea jezoensis(Siebold & Zuccarini) Carriere distributed in Korea. Cone collected from 25 trees in two natural populations(Mt. Jiri and Mt. Dokyu) and their thirteen cone and seed characteristics as well as three germination behaviors were analyzed. Statistical analysis showed significant differences among populations and among individuals within populations in 9 traits except for seed breadth, seed weight, index of seed wing and mean germination time. Generally, morphological variation of cone and seed wings from Mt. Jiri population has smaller and longer than those of Mt. Dokyu population. Percentage of sound seeds and Tetrazolium test from Mt. Jiri population showed 1.79 and 1.87 higher values than Mt. Dokyu population, respectively. The maximum germination percentage was obtained at the optimum temperature of $20^{\circ}C$ and in this case, mean germination time and germination rate showed 7.5 days and 2.9 ea./day, respectively. In seed germination behaviors, percentage(40.7%) and rate(0.90 ea./day) of germination in Mt. Jiri population were more higher and faster than those of Mt. Dokyu population(17.7%, 0.37 ea./day). According to correlation analysis, P. jezoensis populations with small restricted distribution may have been reduced because seed qualities were correlated with increased levels of inbreeding and disproportion flowering.

• Korean Journal of Plant Taxonomy
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• v.47 no.2
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• pp.137-153
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• 2017

Fruit and seed morphological characteristics were investigated in 36 taxa of 20 genera of Korean Ranunculaceae. As a result, fruits were classified as the achene, berry, or follicle type. In the taxa with achene, the presence of trichomes and stoma on the surface of the fruit and a feature in which the endocarp distinctively develops into a one-layer sclereid were considered as useful characteristics. Moreover, in the taxa with follicles, the composition of the seed coat (bitegmic vs. unitegmic), the cell shape of the seed coat, the presence of trichomes, and the type of seed coat were useful for taxonomic characteristics in Korean Ranunculaceae. Based on the fruit and seed characteristics, Adonis is likely to be classified into Helleboroideae rather than Ranunculoideae, as it has bitegmic ovules. In addition, Enemion is similar to Eranthis in unitegmic ovule and the seed coat structure which has non-sclereid exotesta; therefore, it is reasonable to be placed in Helleboroideae. According to the results of this study, more in-depth research is needed regarding the taxonomic positions of Callianthemum and Actaea, which has the only berry fruit type.