• Journal of Life Science
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• v.9 no.2
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• pp.127-135
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• 1999

Gentiana scabra Bunge var buergeri Max, can be widely used such as cut-flower, ornamental and medicinal plant among the native plants. Microscopic and Scanning electron microscopic observation plant were carried out to estimates the characteristics correlated with germinability and germination process, and the results were as follows. The seeds were germinated just after harvest, and it means that there is no or little dormancy of the seeds. Matured seeds showed higher germinability than those of immatured. As the storage period was longer, immatured seeds showed shorter longevity than those of matured. The average longevity was about 190 days. According to the observation results of morphological changes during the germination, seed coat was expanded with water absorption immediately after seeding, and radicals were observed 2 days after seeding. Root hairs were formed behind the growing tip of the root on 4 days after seeding and cotyledon were unfolded on 6 days after seeding. In the microscopic observation of leaf tissues, it could be easily distinguished between midrib, and leaf blade, and also observed upper epidermis, palisade parenchyma and spongy parenchyma consisted as a sing1e layer. Palisade parenchyma was consisted of a single layer of cell. Stomata were restricted lower surface, arranged as anamocytic type, and surrounded by guard cells. In the microscopic anatomy of stem were consisted of cortical layer, forming a single layer of epidermis and parenchyma, and vascular bundle which was consisted of continuous cylinder of vascular tissues.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.119-125
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• 2015

Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensis Makino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of $F_1$ hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross between rapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed and chinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6% and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seed per silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6, respectively. The morphological phenotypes of $F_1$ hybrid plants obtained from seeds in the cross between rapeseed and chinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation of leaves of $F_1$ hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parent species, but color of leaves was dark-green.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.729-732
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• 2000

Antifungal activities of domestic plants were examined to develop natural anti-microbial agents, able to protect onion from spoiling microorganisms during their storage. A fungus was isolated from spoiling onion and identified to Penicillium sp. ON-211, based on the morphological characteristics of conidiophore and conidia. Chloroform extracts of Juniperus chinesis Linnaeus, Juniperus. chi. var. horizontalis, water extract of Thujs orientalis Linnaeus' seed and the n-hexane extract of Juniperus. chi. var. horizontalis N. showed remarkable antifungal activties against this fungus.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.184-187
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• 2003

Verticillium wilt was first observed in 2001 on potatoes (Solanum tuberosum) cv. Superior at Daegwallyong area, one of the major seed potato producing areas in Korea. The wilted potato plants showed typical symptoms including gradual yellowing and interveinal necrosis. There was discoloration in the vascular tissues of the infected stems which turned light brown. Fungal isolates from discolored vascular tissues were whitish to creamy with folding on potato dextrose agar medium, where they used to produce resting dark mycelia but no micro-sclerotia. Conidiophores were septate with side branches, swelled at the base, and arranged in a whorl. Conidia were 2.5-11.2$\times$2.0-4.5 $\mu\textrm{m}$ um in size and were borne in small clusters at the tips of phialides. Optimal temperature range for mycelial growth was $25-30^{\circ}C$. Based on these cultural and morphological characteristics, the fungus was identified as Verticillium albo-atrum Reink & Berth. Pathogenicity tests by root dipping method revealed that the fungus caused the same symptoms as observed in naturally infected potato plants. This is the first report of Verticillium wilt on potato caused by Verticillium albo-atrum in Korea.

• Plant Resources
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• v.2 no.2
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• pp.127-132
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• 1999

This study was carried out to investigate the morphological characteristics of the plants and seeds of Ainsliaea acerifolia and to determine the optimum condition for producing sprouts from the seeds. Plant height, flower stalk length, and pod number were higher in natural habitat than in campus farm. Average 1.2 seeds per pod was set but only 0 to 2 seeds per plant was set in plants with the enveloped flower stalks, indicating that this is an outcrossing species. Most of seeds were 9-11mm long and 1.1-1.4mm wide. Fresh weight of seeds was ranged from 10mg to 17mg. Seeds germinated well at 15$^{\circ}C$ and 2$0^{\circ}C$. Mean germination period was 11.5 day at 15 to $25^{\circ}C$. Sprouts grown at 15$^{\circ}C$ was longest(5.4cm) and heaviest(738mg/10 sprouts). Chlorophyll content was 333mg per fresh weight 110g. Protein, Fe, vitamin Bl, vitamin B2, and vitamin C were respectively 23.7mg, 6.4mg, 1.82mg, 0.49mg, and 10..7mg.

• Journal of Life Science
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• v.20 no.1
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• pp.22-26
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• 2010

Seven rice germplasms with red pigmentation within the pericarp were isolated from a large mutant collection. These red pericarp phenotypes resulted from the functions of the Rc, Rd and RdRc genes. Among them, two brown pericarp of the Rc type, four red pericarp of the RdRc type, and one white pericarp of the Rd type were identified. Morphological and agronomic characteristics of those rice germplasms were studied. The Rc type germplasms have the faint red or brown color pericarp and the Rd types produce the white pericarp, whereas the RdRc type germplasms have the dark red pericarp. Most of the important agronomic characteristics including plant stature, tillering ability, spikelet fertility, and total grain yield were lower in the colored rice than those of the wild-type control. All of the studied colored rice germplasms had a tendency of easy seed-shattering in comparison to the control. These characteristics of newly identified germplasms will be useful for identifying the genes responsible for pericarp color phenotype determination.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.913-920
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• 2010

This study was conducted to improve the seed germination by low temperature and $GA_3$ in $Orostachys$ $japonicus$ A. Berger (Jirisan) and $O.$ $iwarenge$ (Mak.) Hara (Jejuyeonhwa), Korean native plants. Observation of the seeds using a stereo microscope showed that all seeds of the two species have wrinkled surface and oblong shape. Seed size ranged 0.77-1.00/0.25-0.37 mm (length/width), indicating that the seeds are minute seeds. When the seeds of two $Orostachsis$ species were sown into petri-dish and placed in a plant growth chamber of 10, 15, 20, or $25^{\circ}C$, 'Jirisan' showed seed germination below 20% at all temperatures and 'Jejuyeonhwa' 80% at only $10^{\circ}C$. Seed germination of 'Jirisan' increased up to 44% at $10^{\circ}C$ by low temperature ($4^{\circ}C$) storage for 10 days, but decreased again at storage for more than 20 days. The seeds of 'Jejuyeonhwa' showed a large increase in seed germination by low temperature for 20-30 days, which was 95% at $10^{\circ}C$, but low temperature for more than 40 days significantly decreased seed germination. Dipping treatment in GA3 solution of $50-400mg{\cdot}L^{-1}$ for different periods (3, 6, 12, and 24 hrs) remarkably improved germination rate and speed in both species, 80-100% in 'Jirisan' and 90-100% in 'Jejuyeonhwa' at all concentrations and dipping times used in this study.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.79-86
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• 2024

This study was conducted to determine the conditions for breaking dormancy in the seeds of the North Korean plant resource, Trollius macropetalus, and to investigate its germination characteristics. The morphological characteristics and viability of T. macropetalus seeds were examined. Germination characteristics were investigated based on temperature conditions (15/6℃ & 25/15℃), cold stratification treatment, and GA₃ treatment. The results showed that cold stratification treatment had no effect on breaking the dormancy of T. macropetalus. GA₃ treatment significantly increased the germination rate of T. macropetalus and also improved the mean germination days and germination speed. However, compared to the 15/6℃ condition, the effectiveness of GA₃ treatment for T. macropetalus decreased under the 25/15℃ condition. It was determined that T. macropetalus have a nondeep-type morpho-physiological dormancy. The most effective conditions for breaking dormancy were found to be the 15/6℃ temperature condition and GA₃ treatment at 500 mg·L^-1. The findings of this study are expected to provide valuable information for the mass propagation of T. macropetalus, which has potential applications in landscaping and pharmaceutical materials.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.329-335
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• 2014

This study was performed to study characteristics of new zoysiagarass cultivar 'Green Ever' (Plant Variety Protection Application no. 2014 - 02). 'Green Ever' was developed by selection breeding among 100 native zoysiagrasses collected in South Korea. 'Green Ever' showed specific bands (1.48 kb with OPC14 and 1.05 kb with OPD5) which were distinct from 'Anyang Joonggi' or 'Dongrae Koryogi' in RAPD analysis. 'Green Ever' was classified as Z. matrella in morphological evaluations including plant height ($6.8{\pm}0.5cm$), leaf width ($2.0{\pm}0.1mm$), height of lowest leaf ($1.9{\pm}0.1cm$) and seed length ($3.0{\pm}0.1mm$), which was similar with morphological characteristics of 'Dongrae Koryogi'. Turf quality, shoot density and chlorophyll content of 'Green Ever' were higher than them of 'Anyang Joonggi' and 'Dongrae Koryogi' under intensive management such as fairway on golf course, especially shoot densities of 'Green Ever' were excellent higher than 'Anyang Joonggi' and 'Dongrae Koryogi' during experimental periods. It could be extensively used in fairway, teeing ground of golf courses and landscape garden after further study about various environmental adaptabilities such as winter kill, wear tolerance etc.

• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.169-176
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• 2016

Although chemical fertilizers have a quick effect and broad applicability to agricultural fields, they have caused many problems like increasing soil acidity or decreasing soil organic matters. Environmental-friendly agriculture has been attempted in various ways such as organic agriculture, natural farming, low input and sustainable agriculture. The common interest of all environmental-friendly systems is to decrease burden to agricultural environment by low input of agricultural labor and materials. This study was conducted to estimate overwintering capacity and genetic distance among Chinese Milk Vetch (Astragalus sinicus, CMV) collections based on morphological characteristics and AFLP (Amplified fragment length polymorphism) analysis. Furthermore, the effect of CMV as green manure was observed in mix-cultured paddy fields with rice, sesame and sweet-potato. An another objective of this study was also to compare the pattern of weed occurrence in paddy fields with or without CMV and different rice transplanting times. The CMV collected from Paju district in central region of Korea was successively occurring through self-reseeding without artificial management. However, there was no noticeable difference in growth habit between Paju native CMV and introduced CMV from China which is currently used in farm fields. On the basis of multi-dimensional scaling and tree analyses, there are no significant difference of agricultural growth characteristics among Paju and chinese collections only excepting leaf angle and root length. The flowering time of Gurye collection was fast for 1 week as compared to other collections. AFLP that was commonly used for plant classfication, was applied to exam the genetic variation of CMV collections. Total 579 PCR products and 336 polymorphic fragments were generated using 8 primer pairs.