• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.112-121
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• 1988

Soybean seed coat consisted of three layers, and the aleurone layer was attached to the innermost part of seed coat. It showed the different morphological characteristics with single cell layer compared with many cell layers in barley aleurone layer. The structural difference in aleurone cell among varieties was not detected in this experiment. The hole of middle part of hilum consisted of net formed material in order to pass water and gas. In the experiment, it was not studied whether the varieties with hard seed consist of the same structure or not, but the detailed study on this would be necessary. The activity of acid phosphatase showed a tendency to increase in process of imbibition in distilled water. There was no significant difference in the enzyme activity among the varieties tested, but the enzyme activity of Danyoupkong was slightly higher than that of Hwanggeumkong. In germinability, Danyoupkong is higher than Hwangkeumkong, so it might be attributed to the higher activity. There was no difference in acid phosphatase activity between released from the aleurone cell and accumulated in the aleurone cell from 6 to 12 hours incubation of the medium in the absence of GA$_3$, but the difference was detected after 12 hours incubation. And enzyme activity was the highest in the 18 hours incubation. The increase in the release of acid phosphatase from the aleurone cell would be considered as passive diffusive effect due to the increase in turgo pressure of aleurone cell. The acid phosphatase released from aleurone layer increased greatly after 24 hours incubation of the medium in the presence of GA$_3$ and the accumulation within the aleurone cell decreased linearly after 18 hours incubation. The result indicates that GA$_3$ enhance the rate of enzyme release from aleurone layer, suggests that the aleurone cell wall be digested by the introduction of GA and the digested wall act as the channels for enzyme release.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.14-23
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• 2012

This study was performed to investigate the effects of storage, temperature, and pre-treatments on the germination of Melia azedarach seeds collected from Buan, Jeonju, and Jeju provenance. M. azedarach seeds stored with or without pericarp in the ground, which collected from Buan provenance evidenced the highest germination percentage (PG, %) and the shortest time to first germination (days). The seeds collected from Jeonju and Jeju provenance were placed at both six continuous temperatures (15, 20, 25, 30, 35, and $40^{\circ}C$) and two alternating temperatures ($20{\leftrightarrow}30^{\circ}C$ and $25{\leftrightarrow}35^{\circ}C$) for seed incubation. The results showed a significant effect for temperature of seed incubation. The seeds incubated at $35^{\circ}C$ had the highest PG among the continuous temperatures and germinated significantly more at the two alternating temperatures than at $35^{\circ}C$. Concerning mean germination time (MGT), the seeds incubated at $35^{\circ}C$ evidenced the shortest germinations among the continuous temperatures while those at the alternating temperatures germinated for a shorter period than those at $35^{\circ}C$. The germination rate (GR) and germination performance index (GPI) were similar to PG. The seeds collected from Jeonju provenance were treated using five pre-sowing treatments (scarification, scarifcation+$GA_3$, scarification+$KNO_3$, $GA_3$, and $KNO_3$) prior to the germination experiments. Compared with the intact seeds (control), most of the pre-treatments were significantly (especially scarification+$GA_3$ 100 ppm and scarification+$KNO_3$ 1.0%) higher in PG, GR, and GPI, as well as shorter in MGT.

• Journal of Plant Biology
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• v.39 no.3
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• pp.167-172
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• 1996

Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.359-367
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• 2014

This study was carried out to determine the effects of seed storage methods and pre-treatments on seed germination, soil types and shading conditions on potted seedlings growth of S. sieboldiana var. pendula and S. sieboldiana for. xanthocarpa, endemic species in Korea. The seed germination rate of S. sieboldiana var. pendula was the highest at 48.6% when seeds were treated with stratification for 60 days and then soaked in 100 ppm $GA_3$. And seed germination rate of S. sieboldiana for. xanthocarpa was the highest at 51.4% when seeds were pre-chilled for 30 days and then soaked in 100 ppm $GA_3$. And potted seedlings of S. sieboldiana var. pendula showed the best quality under 50% shading in bedsoil with the growth characteristics of plant height (41 cm), number of leaves(8), leaf width (16 cm), leaf length (18 cm), root length (22 cm), fresh weight(aerial part/root part; 20/6.9 g) and dry weight(aerial part/root part; 4.5/2.0 g). And potted seedlings of S. sieboldiana for. xanthocarpa showed the best quality under non-shading in bedsoil with the growth characteristics of plant height (39 cm), number of leaves(10), leaf width (12 cm), leaf length (15 cm), root length (22 cm), fresh weight (aerial part/root part; 21/13 g) and dry weight (aerial part/root part; 4.2/2.2 g). Therefore, seeds treated with stratification or prechilling and then soaked in $GA_3$ were effective in germination of S. sieboldiana var. pendula and S. sieboldiana for. xanthocarpa, and potted seedlings should be cultivated in bedsoil under non or 50% shading condition.

• Korean Journal of Plant Resources
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• v.35 no.6
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• pp.796-804
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• 2022

The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15℃) and intermediate temperature stratification (A, autumn temperature, 15/10℃) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 mg/L GA₃ and GA₄₊₇ treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.1
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• pp.59-69
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• 2000

The present study was undertaken in investigate the response to salinity and effect of plant growth regulators and proline under salinity stress on the germination and seedling growth of Vigna angularis. The protective effect of external Ca2+ on root elongation under saline conditions was also investigated. The seed germination of Vigna angularis decreased with an increase in salinity. The growth regulators GA3 was more effective than kinetin. At a higher salinity, low concentrations of kinetin and high concentrations of GA3 were more effective. The external application of proline and betaine improved germination under saline conditions. At a low salinity proline and betaine alleviated the salinity-induced inhibition of germination, yet at higher NaCl concentrations, proline and betaine were both ineffective. Exposure to salinity during germination was accompanied by an increase in the proline content, thereby suggesting that one compatible solute in the germinating seed would seem to be proline. The inhibition of germination by high NaCl concentrations was relatively more severe in scarified seeds than in intact seeds, indicating that the seed coat acts as a partial barrier to an Na2+ ameliorated the adverse effect of salinity stress.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.200-200
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• 2004

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.8-13
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• 1985

Egremont russet 사과 품종(品種)의 종자에 여러가지 종피(種皮) 파상(破傷)을 한 후 발아(發芽), 유아(幼芽), 유근(幼根)의 생장(生長)과 생리적(生理的)인 왜화(矮花)에 대(對)하여 조사한 결과 다음과 같은 결과를 얻었다. 자엽(子葉)의 크기를 1/2로 절단하고 종피(種皮)를 제거(除去)한 Egremont russet 사과 품종(品種)의 종자(種子)에 $GA_3$를 처리한 것은 평균발아율(平均發芽率)이 55%였으며 동일조건(同一條件)에서 대조구(對照區)는 45%였다. 그리고 종피(種皮)가 완전(完全)한 것과 종피파상(種皮破傷)을 한 구(區)에서는 낮았다. 유아장(幼芽長)은 종피(種皮)를 완전히 남긴 것과 파상(破傷)한 구(區)에 $GA_3$를 처리하였을 때 촉진(促進)되었으나 전체적(全體的)으로 볼 때 종피(種皮)를 제거(除去)한 구(區)에서 더 길었다. 그러나 유근장(幼根長)은 $GA_3$처리에 의(依)해서 억제(抑制)되었으나 유근(幼根)의 직경(直徑)은 더 커졌다. 그리고 생리적(生理的)인 왜화(矮花)는 종피(種皮)를 남긴 구(區)에서 발생(發生)되었으며 $GA_3$ 처리에 의(依)해 극복되지 않았다. 자엽(子葉)의 녹색화(綠色化) 속도는 종피(種皮)를 제거(除去)하고 자엽(子葉)의 크기를 1/2로 절단한 구(區)에서 촉진(促進)되었다.

• Proceedings of the Korean Society of Crop Science Conference
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• 1997.05a
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• pp.88-89
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• 1997

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.64-67
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• 1996

This experiment was carried out to obtain the information on seed dormancy, germinability during storage in wasabia japonica Matsum. $GA_3$ 100ppm+BA 10ppm was more effective than $GA_3$100ppm alone in breaking the dormancy. Storage of seeds pretreated with growth retardants (CCC, Uniconazol) seemed to be a useful method for preventing germination during storage. The stratification was an effective storage method to germinate the seeds for sowing them in fall.