• Fisheries and Aquatic Sciences
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• v.25 no.12
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• pp.619-625
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• 2022

The study carried out a 15-day feeding trial of the fleshy shrimp broodstock that have just finished their first spawn using five different maturation feed types (three types of manufactured feed, fresh feed, and commercial feed) to induce multiple spawning in the shrimp and then analyzed and compared hemolymph levels of total protein, total lipid, hemocyanin, glucose, and cholesterol as well as ovary histology between the treatments to identify the potential of the experimental diets to be utilized as maturation feed. The study found that the manufactured diets of polychaetes + clam and polychaetes + clam + squid resulted in significantly higher levels of lipid and protein in shrimp hemolymph suggesting an indicator of multiple spawning in the fleshy shrimp. The other manufactured diet of polychaets + squid led to the highest 2nd spawning with significantly lower levels of lipid, protein, and cholesterol in shrimp hemolymph. The fresh feed treatment showed similar hematological as well as histological responses to the manufactured diet of polychaetes + squid, but with a significantly lower spawning volume compared to the other treatments. The study demonstrated that the manufactured diets could replace fresh feed as maturation feed and attain a higher shrimp maturation than the commercial feed.

• Development and Reproduction
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• v.14 no.1
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• pp.21-25
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• 2010

Nile tilapia (Oreochromis niloticus) are mouth-brooders so that the females holding eggs in their mouth sacrifice their somatic growth for reproduction. For this reason, artificial control of reproduction for the culture of this species has been of interest. Manipulation of photoperiod is an emerging technique for such purpose, but little information is available to establish appropriate photoperiod regime. To obtain necessary basic information, sexually mature females were individually accommodated to glass aquarium, and the spawning activity of these females were monitored for two years under natural photoperiod regime. Female tilapia spawned most frequently on March, April and May when the day length gradually increased from 11 hours to 14 hours and least frequently on September, October, November and December when the day length gradually decreased from 13 hours to less than 10 hours in the first year. The decrease of spawning frequency as day length decreased was also observed in the second year, although the increase of spawning frequency as day length increased was less clear. Spawning of female tilapia was less active when the night was dark due to the disappearance of moonlight (Dark Phase), compared to the Phase of Getting Lighter, Light Phase and Phase of Getting Darker. Results from this study suggest that long day length, particularly increasing phase, is favoured for active spawning of Nile tilapia, and that this species, as a tropical fish species, may utilize changing lunar phases as a secondary environmental cue for reproduction.

• Ocean and Polar Research
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• v.31 no.3
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• pp.257-264
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• 2009

Seventeen females of the mud crab Scylla serrata, from the State of Kosrae, Micronesia, were transported to the Fisheries Resources Research Institute, Gyeongsangnam-do, in oxygen-filled plastic bags. After acclimatization to a $30^{\circ}C$ holding temperature, nine females were selected for seed production trials. Spawning was hastened using eyestalk ablations; however, this may not be required in commercialscale mud crab seed production. Primary spawning produced an average of 2.4 million hatched larvae, whereas secondary spawning produced 0.4 million. About 10 days elapsed between spawning and hatching and 30 days between hatching and crablet. Mass mortalities up to 90% were observed between stages zoea 1 and zoea 2 in every trial. The highest survival rate from zoea 1 to crablet was estimated at 0.25%. Most commercial shrimp hatcheries in Korea are equipped with almost all necessary facilities and could be converted easily to mud crab hatcheries, able to run three to four times per year using hatchery technologies developed for blue crabs and Chinese mitten crabs.

• Korean Journal of Ichthyology
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• v.28 no.2
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• pp.72-78
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• 2016

The age and spawning characteristics of Iksookimia pacifica were investigated in this study to provide baseline data for its ecological characteristics in the Bukcheon (stream), Goseong-gun, Gangwon-do, Korea from January to December, 2013. Age groups of I. pacifica (female) estimated by the frequency distribution of total length in the spawning season indicated that the 30~49 mm group is 1 year old, the 50~74 mm group is 2 years old, the 75~99 mm group is 3 years old, the 100~124 mm group is 4 years old, and the 130~160 mm group is $5{\leq}$years old. The sex ratio (♂/♀) was 0.63, and females were 30~60 mm larger than males. The lamina circularis at the base of pectoral fins in males as a secondary sexual character was created at the 13th month after hatching. The spawning season according to the gonadosomatic index (GSI) was July to August (main spawning season in July) with water temperatures of $20{\sim}25^{\circ}C$. The average number of eggs was $2,503{\pm}1,337$ with a diameter of $1.11{\pm}0.04mm$ in mature eggs.

• The Korean Journal of Malacology
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• v.31 no.2
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• pp.113-121
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• 2015

Gametogenesis, mating behaviour and spawning of Octopus ocellatus were investigated by histological study. This species is dioecious, and showed a protandry phenomenon. Ooogenesis (in females) and spermatogenesis (in males) can be classified into 3 stages, respectively. O. ocellatus copulates in one of two ways: a male may leap upon a female, mounting her mantle, or a male may sit near the female and extend the hectocotylized third right arm toward her. Spawning occurred between April and June in females, and between March and May in males of O. ocellatus. The spawning period was once a year and the peak took place between May and June. A number of flatened follicle cells, which were attached to an oocyte, were involved in vitellogenesis in the cytoplasm of the vitellogenic oocyte (maturing oocyte), and formation of chorion membrane (secondary egg membrane) of the ovarian eggs. Fecundity per female closely related to GSI was 294-660 eggs (average, 429 eggs). The diameters of the ovarian eggs surrounded by chorion membrane were approximately in the range of 10.10-2.50 mm. Each ovarian egg laid by a female was connected to an egg string. Each egg string was 1-5.5 cm (average 3.6 cm). The total number of eggs laid by a female of this species ranged 218-314, the egg sizes were independent to the size of female adult. this species has a life mode showing some special reproductive characteristics of an annual semelparity as shown in Octopodidae species because we have never seen a female spawning a second time.

• Development and Reproduction
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• v.1 no.2
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• pp.181-187
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• 1997

This study has been conducted to investigate the mating behavior, time sequence of mating and time limit with which female can fulfill spawning and brooding after pre-spawning molting in the giant fresh-water prawn, macrobrachium rosenbergii reared in the laboratory. the results obtained were summarized as follows. Mating happened spontaneously as the following sequences: courting gesture, seizure of female by male, mounting, turning of female, copulation. The entire mating lated approximately seven minutes. The endopodite of 2nd pleopod of male bore the appendix masculins whcih are male's secondary sex characteristic. cinciulli was formed at the distal portion of appendix interna which are on the upper portion of appendix masculins and helped stabilizing the connection of each side of 2nd pleopod during mating. After the connection of each side of 2nd plepod was fixed on the posterior portion of the thoracic sternum in female, a gelatinous spermatophore that emitted at the basipodite of 5th pereiopod during courting gesture was deposited on the ventral groove of 2nd, 3rd, 4th and 5th pereiopod. time limit which female can fulfill copulation and brooding was from 3 hours to 15 hours after pre-spawning molting.

• Korean Journal of Ichthyology
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• v.23 no.2
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• pp.158-162
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• 2011

Growth and spawning ecology of Cobitis lutheri (Teleoste: Cobitidae) were investigated in the Samcheon Stream, Mangyeong River, Jeollabuk-do, Korea from 2005 to 2006. Age group of C. lutheri estimated from the total length indicated that the 40 ~ 60 mm group is 1 year old, the 60 ~ 80 mm group is 2 years old, and the 80 ~ 110mm group is 3 ${\leq}$ years old. The sex ratio (Male/Female) was 0.78, and the female was 20 ~ 30 mm larger than the male. Lamina circularis at the base of pectoral fins in the males as a secondary sexual character was created 12 months after hatching. A change of lateral coloration of males was observed from April to September. The spawning season was early to middle July with water temperatures of $24{\sim}26^{\circ}C$. The average number of eggs was $1,127{\pm}453$ with the egg diameter of $1.09{\pm}0.02$ mm.

• Korean Journal of Ichthyology
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• v.18 no.2
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• pp.141-147
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• 2006

The matured female and male of A. signifer differed in external morphology and body color. There were significant differences in PAD, PAB, PMP and PQR between matured female and male. Green color was very widely distributed in whole as nuptial color, that is similar to green and cyan color in the body part, yellow color in the ventral part and red and yellow color in the tail part. The nuptial organ of A. signifer was formed in a cluster on the horny substance of both sides above the mouth. There were 25~30 nuptial organs in a side. The nuptial organs disappeared with crumbling down from middle part of nuptial organ after spawning period. The nuptial organs disappeared mostly in September as non spawning period and a part of the nuptial organs remained as a vestige in the edges of both sides.

• Korean Journal of Ichthyology
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• v.28 no.4
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• pp.239-248
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• 2016

The habitat, age and spawning characteristics of the Korean eastern spined loach, Iksookimia yongdokensis, were investigated to obtain baseline data for its ecological characteristics in the Chuksancheon (stream), Yeongdeok-gun, Gyeongsangbuk-do, Korea. The difference between the habitats of juveniles (0+) and 1~4 year old loaches was that juveniles mainly inhabited sandy bottoms (substratum particle size $0.6{\pm}1.44\;cm$) with very slow velocities of water current ($2.4{\pm}2.09\;cm/sec$) and low depths ($29.2{\pm}7.57\;cm$), whereas 1~4 year old loaches inhabited pebble and cobble bottoms (substratum particle size 5.5~6.1 cm) with relatively fast velocities of water current (3.8~4.6 cm/sec) and deep depths (50~70 cm). The age groups for I. yongdokensis (female) estimated by the frequency distribution of total length in the spawning season (July) indicated that the 35~64 mm is 1-year old, the 65~94 mm group is 2-year old, the 95~119 mm is 3-year old, and the 120~145 mm is more than 4-year old. The sex ratio (♂/♀) was 0.69, and females were 10~30 mm larger than males. The lamina circularis at the base of the pectoral fins in males, a secondary sexual characteristic, formed at the 13th month after hatching. The spawning season according to the gonadosomatic index (GSI) was June to July with water temperatures of $20{\sim}25^{\circ}C$. The average number of eggs was $2,292{\pm}618$, with a diameter of $1.46{\pm}0.02\;mm$ for mature eggs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.1
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• pp.48-55
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• 2009

The gonad maturation and spawning of blue crab, Portunus trituberculatus (Miers, 1876) using samples caught by trammer net in the western coast of Korea from January to December. The gonadosomatic index (GSI) showed the highest value of 15.0 in June and lowest value of 1.29 in August. Hepatosomatic index (HSI) showed the lowest in the spawning season from June to July. Thereafter, from August the value started to increase to 5.77 in December, showing an adverse trend against GSI. In the histological studies of the female reproductive organ, the gonad filled with primary oocytes from January to February and secondary oocytes in May. The female gonads mature from June, and mature and immature groups could be distinguished from December to February or March. In August, female developed another cycle of gonad development, showing new primary oocytes in the gonads again. Some of female crabs had receptaculum filled with already active sperm cells in it from March to May. In the time of August to September, almost all the females had the sperm sacs in the receptaculum. From the result, the coupling period estimated from August to September. Total RNA and DNA values of the crabs were highest in May (2.51, $0.57{\mu}g/mg$). The DNA value remained unchanged except for the value in May ($0.09-0.13{\mu}g/mg$). while RNA value was lowest in January ($1.08{\mu}g/mg$). The RAN/DNA ratio was the lowest (6.23%) in May and highest (18.05%) in July. The value of correlations coefficient between body weight and fecundity of the crabs was higher that of between carapace width and fecundity.