• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.267-270
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• 2000

In general, pigment production can be influenced by the medium composition, pH and physical factors such as aeration, agitation, and visible light. The influence of gaseous environments on the pigment production by Monascus purpureus ATCC 16365 was investigated by controlling the DO (dissolved oxygen) concentration through aeration and agitation. When the DO concentration was controlled below 20%, the production of red pigment significantly increased whereas the biomass production decreased. Therefore, the dissolved oxygen concentration could significantly affect the biosynthesis of red pigment as a secondary metabolite by a wild-type filamentous fungus under the anaerobic condition. The results indicate a high potential of enhancing the productivity of the red pigment as a secondary metabolite through controlling the DO concentration.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.417-420
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• 2005

Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, we applied several abiotic elicitors in order to enhance growth and ginseng saponin biosynthesis in the hairy roots of P. ginseng. Generally, elicitor treatments were found to inhibit the growth of the hairy roots, although simultaneously enhancing ginseng saponin biosynthesis. The addition of selenium at inoculum time did not significantly affect ginseng saponin biosynthesis. However, when 0.5 mM selenium was added as an elicitor after 21 days of culture, ginseng saponin content and productivity increased to about 1.31 and 1.33 times control levels, respectively. These results suggest that processing time for the generation of ginseng saponin in a hairy root culture can be reduced via the application of an elicitor.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1597-1600
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• 2003

Fine-Blanking is an advanced and precision stamping process, by which a component with precise geometry and smooth cut surface can be produced without any further major secondary operations. By applying the Fine-Blanking technology, the significant improvement of the component should be obvious. As the components are with good shape, smooth surface and precise size, they can be ready for assembly without any further secondary operations. The productivity is increased, the production cycle time and the component cost are significantly reduced. We apply the fine-Blanking for chain sprocket. And do Mecanical test for compress strenth. impaact, roughness, Brinell hardness, dimensional stability.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.665-668
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• 2006

Concrete secondary product that use cement is increasing application from reason of shortening etc.. of construction period in construction site. Manufacture method of construction of this concrete secondary product there is hot-check method, direct spray method, press method, extrusion molding method etc.. Also, I am using reinforcement boating certainly in this process. In most case, We have used asbestos by reinforcement fiber until early 90s but use from human body hurtfulness controversy is felt constraint. Therefore, application of principal parts fiber is increasing. But, to replace asbestos, because must satisfy all lubricating ability, productivity etc.. class, it is the very difficult matter to replace asbestos. In this study, I wished to do Test about asbestos principal parts possibility at extrusion process to charge shape or form making test piece because mixs polypropylene fiber etc. by plan that replace asbestos in cement extrusion molding product and measures bending strength and elasticity.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.10
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• pp.1078-1088
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• 2012

In this paper, we present research experimental results about the different thickness T-joint welding of the high power continuous wave(CW) Nd:YAG laser for the secondary battery of a vehicle. Although the conventional method used for the secondary battery is a argon TIG welding, we utilize a laser welding to improve Tungsten Inert Gas(TIG) welding's weakness. The laser, which has a couple of advantage such as aspect ratio, low Heat Affected Zone(HAZ), good welding quality and fast productivity utilized in this work is a CW Nd:YAG laser. In order to observe laser welding sections, we used a optical microscope. Through the analysis of the metallographic, hardness, aspect ratio, and heat input, we obtained the desired data in condition of 1800 W laser beam power and 1.8 m/min and 2.0 m/min laser beam travel speeds. In order to compare electric resistances of the argon TIG welding and laser welding, we made an actual battery and the electric resistance of the laser welding is reduced by 40~45% comparing with the argon TIG welding.

• KSBB Journal
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• v.13 no.2
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• pp.203-208
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• 1998

We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), $Y_x/s$ (0.49 g DCW/g fructose), $Y_p/s$<(22.56 mg CyA/g fructose), and YTEX>$_p/x$ (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism.

• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.186-192
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• 2021

The roots of Korean ginseng (Panax ginseng) have a long history of usage as a medicinal drug. Ginsenosides, a group of triterpenioid saponins in ginseng, have been reported to show important pharmacological effects. Many studies have attempted to identify the ginsenoside synthesis pathways of P. ginseng and to increase crop productivity. Recent studies have shown that exogenous gibberellin (GA) treatments promote storage root secondary growth by integration of the modulating cambium stem cell homeostasis with a secondary cell wall-related gene network. However, the dynamic regulation of ginsenoside synthesis-related genes and their contents by external signaling cues has been rarely evaluated. In this study, we confirmed that GA treatment not only enhanced the secondary growth of P. ginseng storage roots, but also significantly enriched the terpenoid biosynthesis process in RNA-seq analysis. Consistently, we also found that the expression of most genes involved in the ginsenoside synthesis pathways, including those encoding methylerythritol-4-phosphate (MEP) and mevalonate (MVA), and the saponin content in both leaves and roots was increased by exogenous GA application. These results can be used in future development of biotechnology for ginseng breeding and enhancement of saponin content.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.1
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• pp.47-52
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• 2005

This study was conducted to evaluate the performance of submerged hollow fiber MF processes to treat secondary wastewater for water reuse. Specifically, membrane productivity and filtrate water quality were investigated under various operating conditions (i.e. flux, recovery, and backwash rate) at pilot-scale. Membrane fouling became more severe with increasing flux and recovery, suggesting that low flux operation (< 25 LMH) was desirable. At high flux operating(> 37.5 LMH), increasing backwash rate showed only limited success. The biofouling, quantified by PEPA and BFHPC, was also significant in wastewater reclamation, and biogrowth control by chlorine, were necessary to improve membrane productivity. Filtrate water qualities are in good compliance with water reuse regulations regardless of operating conditions (flux, recovery and backwash rate). Particle (e.g. turbidity) removal ranged from 89 to 98%, while only 11 to 21% of organics (e.g. NPDOC) were removed by MF membrane. Only small improvement in biostability (e.g. AOC) was achieved by MF system, and thus, without post disinfection, significant microorganisms might be present in the filtrate due to regrowth. Lastly, in order to further investigate pathogen removal, controlled microbial challenge tests were performed by monitoring Giardia, Cryptosporidium, bacteria and virus, and showed relatively good microbial removal.

• Journal of Korean Academy of Nursing
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• v.20 no.1
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• pp.16-37
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• 1990

A cost analysis for hospitalized patients was carried out based upon Patient Classification System(PCS) in order to determine an appropriate nursing fee. The data were collected from 21 nursing units of three teaching hospitals from April 1 to June 30, 1989. first, all of the 22,056 inpatients were classified into mildly ill(Class Ⅰ), moderately ill(Class Ⅱ), acutely ill(Class Ⅲ), and critically ill(Class Ⅳ) by the PCS which had been carefully developed to be suitable for the Korean nursing units. Second. PCS cost accounting was applied to the above data. The distribution of inpatients, nursing costs, and nursing productivity were as follows : 1) Patient distribution ranged from 45% to class Ⅰ, 36% to class Ⅱ, 15% to class Ⅲ, and 4% to class Ⅳ, the proportion of class Ⅳ in ‘H’ Hospital was greater than that of the other two hospitals. 2) The proportion of Class Ⅲ and Ⅳ in the medical nursing units was greater than that of surgical nursing units. 3) The number of inpatients was greatest on Tuesdays, and least on Sundays. 4) The average nursing cost per hour was W 3,164 for ‘S’ hospital, W 3,511 for ‘H’ hospital and W 4,824 for ‘K’ hospital. The average nursing cost per patient per day was W 14,126 for ‘S’ Hospital, W 15,842 for ‘H’ hospital and W 21,525 for ‘K’ hospital. 5) The average nursing cost calculated by the PCS was W 13,232 for class Ⅰ, W 18,478 for class Ⅱ, W 23,000 for class Ⅲ, and W 25,469 for class Ⅳ. 6) The average nursing cost for the medical and surgical nursing units was W 13,180 and W 13,303 respetively for class Ⅰ, W 18,248 and W 18,707 for class Ⅱ, W 22,303 and W 23,696 for class Ⅲ, and W 24,331 and W 26,606 for class Ⅳ. 7) The nursing costs were composed of 85% for wages and fringe benefits, 3% for material supplies and 12% for overhead. The proportion of wages and fringe benefits among the three Hospitals ranged from 75%, 92% and 98% for the ‘S’, ‘H’, ‘K’ hospitals respectively These findings explain why the average nursing cost of ‘K’ hospital was higher than the others. 8) According to a multi- regression analysis, wages and fringe benefits, material supplies, and overhead had an equal influence on determining the nursing cost while the nursing hours had less influence. 9) The productivity of the medical nursing units were higher than the surgical nursing units, productivity of the D(TS) - nursing units was the lowest while the K(Med) - nursing unit was the highest in 'S' hospital. In ‘H’ hospital, productivity was related to the number of inpatients rather than to the characteristics of the nursing units. The ‘K’ hospital showed the same trend as ‘S’ hospital, that the productivity of the medical nursing unit was higher than the surgical nursing unit. The productivity of ‘S’ hospital was evaluated the highest followed by ‘H’ hospital and ‘K’ hospital. Future research on nursing costs should be extended to the other special nursing areas such as pediatric and psychiatric nursing units, and to ICU or operating rooms. Further, the PCS tool should be carefully evaluated for its appropriateness to all levels of institutions(primary, secondary, tertiary). This study took account only of the quantity of nursing services when developing the PCS tool for evaluating the productivity of nursing units. Future research should also consider the quality of nursing services including the appropriateness of nursing activities.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.12
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• pp.853-861
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• 2020

The study aims to measure the productivity of the Saudi banking sector at the retail level using secondary data for 11 local banks from the period 2015-2019. The study uses an extended version of the Cobb-Douglas production function to account for the fact that as banks openup more retail branches, they will need to employ more labor. The extended Cobb-Douglas production function was estimated using the two-way fixed effect model to account for unobserved heterogeneity across Saudi banks resulting from differences in labor competencies and leadership style. Besides, the model accounts for unobserved heterogeneity among Saudi banks due to the advancement in electronic services over time. The results showed that labor, branches, customers' deposits, and fixed deposits have a positive effect on the total value of generated loans. Conversely, ATM has an insignificant effect on generated loans. The average scale elasticity shows that the Saudi banks at the retail level are operating under decreasing returns to scale. The average marginal rate of technical substitution shows that Saudi banks need at least one ATM to replace one unit of labor at the retail level while keeping the same level of output.