• 식물병연구
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• 제29권3호
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• pp.321-329
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• 2023

The bacterium Neobacillus endophyticus BRMEA1^T, isolated from the medicinal plant Selaginella involvens, known as its thermotolerant can grow at 50℃. To explore the genetic basis for its heat tolerance response and its potential for producing valuable natural compounds, the genomes of two thermotolerant and four mesophilic strains in the genus Neobacillus were analyzed using a bioinformatic software platform. The whole genome was annotated using RAST SEED and OrthVenn2, with a focus on identifying potential heattolerance-related genes. N. endophyticus BRMEA1^T was found to possess more stress response genes compared to other mesophilic members of the genus, and it was the only strain that had genes for the synthesis of osmoregulated periplasmic glucans. This study sheds light on the potential value of N. endophyticus BRMEA1^T, as it reveals the mechanism of heat resistance and the application of secondary metabolites produced by this bacterium through whole-genome sequencing and comparative analysis.

• 동의신경정신과학회지
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• 제34권3호
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• pp.151-161
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• 2023

Objectives: To examine effect of Korean medical treatment on patients with insomnia and correlations among changes in psychological scales before and after treatment. Methods: Medical records of 38 patients diagnosed with insomnia based on DSM-V who received Korean medical treatment (herbal-medication, acupuncture, Korean psychotherapy) for at least 8 weeks were retrospectively reviewed. Psychological scales including Insomnia Severity Scale (ISI), Beck Depression Inventory-II (BDI-II), State-Trait Anxiety Inventory (STAI), Beck Anxiety Inventory (BAI), and State-Trait Anger Expression Inventory (STAXI) were measured every four weeks and analyzed. Results: After eight weeks of treatment, ISI, BDI-II, BAI, STAI-X-1/2, and STAXI-S/T showed statistically significant decreases. Psychological scale pairs that showed a statistically significant correlation were ISI and BDI-IIㆍSTAI-X-1ㆍSTAI-X-2ㆍTA, BDI-II and BAIㆍSTAI-X-1ㆍSTAI-X-2ㆍAX-I, BAI and STAI-X-1ㆍSTAI-X-2ㆍSAㆍTA, STAI-X-1 and STAI-X-2, SA and TA, and TA and AX-I. In women, the improvement of AX-C was high. The improvement in AX-I score was significant when the disease duration was more than one year. A lower pretreatment BAI value predicted a greater decrease in ISI score after treatment. Conclusions: Korean medical treatment including herbal medicine, acupuncture, and Korean psychotherapy was effective in improving insomnia symptoms and accompanying symptoms such as depression, anxiety, and anger. In the future, more in-depth follow-up research is needed on the mechanisms by which various psychological problems (depression, anxiety, anger, etc) cause and worsen insomnia and the psychological symptoms secondary to insomnia.

• 대한간호학회지
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• 제53권4호
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• pp.426-438
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• 2023

Purpose: Walking practice is a representative indicator of the level of physical activity of local residents. Although the world health organization addressed reduction in prevalence of insufficient physical activity as a global target, the rate of walking practice in Korea has not improved and there are large regional disparities. Therefore, this study aimed to explore the spatial variations of walking practice and its associated factors in Korea. Methods: A secondary analysis was conducted using Community Health Outcome and Health Determinants Database 1.3 from Korea Centers for Disease Control and Prevention. A total of 229 districts was included in the analysis. We compared the ordinary least squares (OLS) and the geographically weighted regression (GWR) to explore the associated factors of walking practice. MGWR 2.2.1 software was used to explore the spatial distribution of walking practice and modeling the GWR. Results: Walking practice had spatial variations across the country. The results showed that the GWR model had better accommodation of spatial autocorrelation than the OLS model. The GWR results indicated that different predictors of walking practice across regions of Korea. Conclusion: The findings of this study may provide insight to nursing researchers, health professionals, and policy makers in planning health programs to promote walking practices in their respective communities.

• 가정간호학회지
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• 제30권1호
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• pp.48-58
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• 2023

Purpose: The purpose of this study was to construct a nomogram for predicting difficulty in peripheral intravenous cannulation (DPIVC) for adult inpatients. Methods: This study conducted a secondary analysis of data from the intravenous cannulation cohort by intravenous specialist nurses at a tertiary hospital in Seoul. Overall, 504 patients were included; of these, 166 (32.9%) patients with failed cannulation in the first intravenous cannulation attempt were included in the case group, while the remaining 338 patients were included in the control group. The nomogram was built with the identified risk factors using a multiple logistic regression analysis. The model performance was analyzed using the Hosmer-Lemeshow test, area under the curve (AUC), and calibration plot. Results: Five factors, including vein diameter, vein visibility, chronic kidney disease, diabetes, and chemotherapy, were risk factors of DPIVC. The nomogram showed good discrimination with an AUC of 0.81 (95% confidence interval: 0.80-0.82) by the sample data and 0.79 (95% confidence interval: 0.74-0.84) by bootstrapping validation. The Hosmer-Lemeshow goodness-of-fit test showed a p-value of 0.694, and the calibration curve of the nomogram showed high coherence between the predicted and actual probabilities of DPIVC. Conclusion: This nomogram can be used in clinical practice by nurses to predict DPIVC probability. Future studies are required, including those on factors possibly affecting intravenous cannulation.

• 산업융합연구
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• 제21권6호
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• pp.91-97
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• 2023

본 연구는 제8기 국민건강영양조사 자료를 이차 분석한 서술적 조사연구이다. 한국의 30~49세 성인 중 당뇨병 유병자 총 300명의 당뇨병 유병률과 인지율을 분석하고 이에 영향을 미치는 요인을 확인하고자 시도되었다. 자료 분석은 SPSS 25.0 program을 이용하여 복합표본 빈도와 백분율, χ²-test, 로지스틱 회귀분석을 실시하였다. 당뇨병 유병자 중 당뇨병임을 인지한 자는 171명(57.0%)이었으며 인지에 영향을 미치는 요인은 성별, 교육수준, BMI, 주관적 건강상태, 심혈관질환, 부계 및 모계 가족력이었다. 본 연구의 결과를 토대로 비교적 젊은 30~49세 당뇨 유병률을 감소시키고 유병자들의 인지율을 높이는 정책 및 중재 프로그램을 수립하는데 기초자료로 활용될 수 있을 것이다.

• Journal of Korean Biological Nursing Science
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• 제25권2호
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• pp.95-104
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• 2023

Purpose: The purpose of this study was to analyze the trends and characteristics of infection-related patient safety incident reporting before and during the coronavirus disease 2019 (COVID-19) pandemic in Korea, and to provide basic data for preventing infection-related patient safety incidents and improving their management. Methods: A cross-sectional analysis of secondary national data (Patient Safety Reporting Data) was conducted. In total, 517 infection-related patient safety incidents reported from 2018 to 2021 were analyzed. Changes in the number of reports before and during the COVID-19 pandemic and differences in variables related to infection-related patient safety incidents were analyzed using the chi-square test and independent t-test in SPSS 29.0. Results: This study found that infection-related patient safety incidents decreased during the COVID-19 pandemic compared to before the pandemic. Furthermore, incident-related characteristics, such as the type of healthcare organization, severity of harm, and post-incident actions, changed during the COVID-19 pandemic. Conclusion: The many changes in the infection control system and practices during the COVID-19 pandemic may have contributed to a decrease in the reporting of infection-related patient safety incidents. It is hoped that longitudinal studies on patient safety incidents related to the pandemic and analytical studies on factors influencing patient safety incidents will continue to be conducted to prevent and improve patient safety incidents.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.806-822
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• 2023

In the current study we assessed a new crystallized compound, 5-(1-hydroxybutyl)-4-methoxy-3-methyl-2H-pyran-2-one (C-HMMP), from the endophytic fungus Colletotrichum acutatum residing in the medicinal plant Angelica sinensis for its in vitro antimicrobial, antibiofilm, antioxidant, antimalarial, and anti-proliferative properties. The promising compound was identified as C-HMMP through antimicrobial-guided fraction. The structure of C-HMMP was unambiguously confirmed by 2D NMR and HIRS spectroscopic analysis. Antimicrobial property testing of C-HMMP showed it to be effective against a variety of pathogenic bacteria and fungi with MICs ranging from 3.9 to 31.25 ㎍/ml. The compound displayed excellent antibiofilm activity against C. albicans, S. aureus, and K. pneumonia. Furthermore, the antimalarial and radical scavenging activities of C-HMMP were clearly dosedependent, with IC50 values of 0.15 and 131.2 ㎍/ml. The anti-proliferative activity of C-HMMP against the HepG-2, HeLa, and MCF-7 cell lines in vitro was investigated by MTT assay, revealing notable anti-proliferative activity with IC50 values of 114.1, 90, and 133.6 ㎍/ml, respectively. Moreover, CHMMP successfully targets topoisomerase I and demonstrated beneficial anti-mutagenicity in the Ames test against the reactive carcinogenic mutagen, 2-aminofluorene (2-AF). Finally, the compound inhibited the activity of α-glucosidase and α-amylase with IC50 values of 144.7 and 118.6 ㎍/ml, respectively. To the best of our knowledge, the identified compound C-HMMP was obtained for the first time from C. acutatum of A. sinensis, and this study demonstrated that C-HMMP has relevant biological significance and could provide better therapeutic targets against disease.

• Safety and Health at Work
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• 제14권3호
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• pp.279-286
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• 2023

Background: This study aimed to evaluate the association between exposure to occupational hazards and the metabolic syndrome. A secondary objective was to analyze the additive and multiplicative effects of exposure to risk factors. Methods: This retrospective cohort was based on 31,615 health examinees at the Pusan National University Yangsan Hospital in Republic of Korea from 2012-2021. Demographic and behavior-related risk factors were treated as confounding factors, whereas three physical factors, 19 organic solvents and aerosols, and 13 metals and dust were considered occupational risk factors. Time-dependent Cox regression analysis was used to calculate hazard ratios. Results: The risk of metabolic syndrome was significantly higher in night shift workers (hazard ratio = 1.45: 95% confidence interval = 1.36-1.54) and workers who were exposed to noise (1.15:1.07-1.24). Exposure to some other risk factors was also significantly associated with a higher risk of metabolic syndrome. They were dimethylformamide, acetonitrile, trichloroethylene, xylene, styrene, toluene, dichloromethane, copper, antimony, lead, copper, iron, welding fume, and manganese. Among the 28 significant pairs, 19 exhibited both positive additive and multiplicative effects. Conclusions: Exposure to single or combined occupational risk factors may increase the risk of developing metabolic syndrome. Working conditions should be monitored and improved to reduce exposure to occupational hazards and prevent the development of the metabolic syndrome.

• Genomics & Informatics
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• 제21권3호
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• pp.42.1-42.23
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• 2023

Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, one of the most deadly infections in humans. The emergence of multidrug-resistant and extensively drug-resistant Mtb strains presents a global challenge. Mtb has shown resistance to many frontline antibiotics, including rifampicin, kanamycin, isoniazid, and capreomycin. The only licensed vaccine, Bacille Calmette-Guerin, does not efficiently protect against adult pulmonary tuberculosis. Therefore, it is urgently necessary to develop new vaccines to prevent infections caused by these strains. We used a subtractive proteomics approach on 23 virulent Mtb strains and identified a conserved membrane protein (MmpL4, NP_214964.1) as both a potential drug target and vaccine candidate. MmpL4 is a non-homologous essential protein in the host and is involved in the pathogen-specific pathway. Furthermore, MmpL4 shows no homology with anti-targets and has limited homology to human gut microflora, potentially reducing the likelihood of adverse effects and cross-reactivity if therapeutics specific to this protein are developed. Subsequently, we constructed a highly soluble, safe, antigenic, and stable multi-subunit vaccine from the MmpL4 protein using immunoinformatics. Molecular dynamics simulations revealed the stability of the vaccine-bound Tolllike receptor-4 complex on a nanosecond scale, and immune simulations indicated strong primary and secondary immune responses in the host. Therefore, our study identifies a new target that could expedite the design of effective therapeutics, and the designed vaccine should be validated. Future directions include an extensive molecular interaction analysis, in silico cloning, wet-lab experiments, and evaluation and comparison of the designed candidate as both a DNA vaccine and protein vaccine.

• 산업융합연구
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• 제22권1호
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• pp.163-168
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• 2024

본 연구는 중년기성인의 고지혈증 관리 요인을 확인하기 위하여 시도되었으며, 연구방법은 한국의료패널조사 2018년도 자료를 분석한 이차분석연구이다. 연구 대상자는 40세 이상 65세 미만의 성인 중 고지혈증진단을 받은 622명을 대상으로 분석하였다. 연구결과 교육수준과 고지혈증관리 유무가 관련성이 있으며(𝒳²=7.285, p=.026), 대상자가 주관적으로 느끼는 건강상태와 고지혈증관리 유무가 서로 관련성이 있는 것으로 조사되었다(𝒳²=7.295, p=.026). 중년기는 신체적인 노화과정이 본격적으로 일어나는 시기로 만성질환에 이환될 가능성이 높으며, 고지혈증은 만성질환을 유발하는 초기 질환이다. 이에 적극적인 건강관리가 필요하며 특히 대상자의 교육수준과 건강상태에 맞는 고지혈증관리를 위한 차별화된 프로그램 개발이 요구된다.