• 제목/요약/키워드: Second cleavage

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Morphological Changes of the Embryo and First Zoea Larvae of Snow Crab, Chionoecetes opilio (O. Fabricius) (대게 Chionoecetes opilio (O. Fabricius)의 난발생 및 부화 유생의 형태)

  • 임영수;이복규;이종관;허성범
    • Journal of Aquaculture
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    • v.11 no.3
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    • pp.337-344
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    • 1998
  • This paper documented mophological changes of embryonic development and first zoea larvae of snow crab, Chinoecetes opilio. Female crabs were sampled by the Danish seine fishery at the depth about 200m in Sep. 1997 in the eastern coast of Korea. Female with newly berried eggs was reared at the water temperature of 5$^{\circ}C$ till the time of hatching. The results obtained are as follwos. Embryonic development : According to morphogenesis of fertilized eggs, the developemental process of the embryo was classified into the following seven stages : First stage (cleavage and blastula stage, 24 days) Second stage (gastrula stage, 72 days) Third stage (nauplius stage, 22 days) Fourth stage (metanauplius stage, 57days) Fifth stage (stage of a pigmentary deposit in the compound eye, 30 days) sixth stage (chromatophore appearance stage in maxillipede, 56 days) Seventh stage (hatching stage, 36 days) Larvae hatched as prezoeas and they molted to first zoea in about an hour. The first zoea is 4.6 to 5.1mm in length, 3.2~3.6mm in width. The abdomen consists of five segments and a bifurcate telson.

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A Comparative Study of the Welfare State Formation in Korea and Western European Nations; From Pre-modern to Post-modern Era (한국과 서구의 국가복지 발전에 대한 비교사적 검토 : 전통과 탈현대의 사이에서)

  • Hong, Kyung-Zoon
    • Korean Journal of Social Welfare
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    • v.35
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    • pp.427-451
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    • 1998
  • The purpose of this study is (1) to contrast the welfare state formation in Korea with that in Western European nations, and (2) to examine the historical peculiarities of the Korean welfare state formation process. For the analysis, this study uses' contrast of context' logic of comparative history and contrast the process of (1)modern state formation (2) civil society development (3) interventionist state evolution of Korea with those of Western European nations. The findings of this study are as follows: First, the distingushing role of nation state as welfare provider is very different. It is attributed to the difference in the traditional dominance structure and in the nation-bulding process of each case. Second, class cleavage of the Western Eeuropean nations has been continually mobilized for political action and converted into political resources, while it is impossible to achieve such results in Korea which has continued labor excusive regime. Third, the institutionalization patterns of welfare politics are different. In Western Eeuropean nations, public welfare benefits have been able to produce welfare coalitions and politics of solidarity. By contrast, since welfare have been thoroughly depoliticized and informalized in Korea, voters and political parties have not been able to make issues of welfare problem Due to these historical peculiarities, it seems to be impossible that Korea's underdeveloped public welfare could be changeable in near future.

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Characterization of the genomes of Aujeszky's disease virus isolated in Korea (국내분리 오제스키병 바이러스의 게놈 유전자 특성 분석)

  • Hyun, Bang-Hun;Kim, In-Joong;Pyo, Hyun-Mi;Cha, Sang-Ho;Park, Ji-Yeun;Song, Jae-Young;Cho, In-Soo;Yang, Chang-Bum;An, Soo-Hwan;Lee, Joong-Bok
    • Korean Journal of Veterinary Research
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    • v.49 no.1
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    • pp.45-57
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    • 2009
  • The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.

Cloning, Sequencing and Comparison of Genes for early Enzymes of the Protocatechuate (ortho-Cleavage) Pathway in Pseudomonas putida (Pseudomonas putida의 Protocatechuate 경로에 관여하는 초기 효소들의 유전자의 클로닝 및 염기서열 분석비교)

  • Hong, Bum-Shik;Shin, Dong-Hoon;Kim, Jae-Ho
    • Applied Biological Chemistry
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    • v.39 no.6
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    • pp.472-476
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    • 1996
  • The major portions of two DNA fragments, one from degradative plasmid, pRA4000 from Pseudomonas putida NCIMB 9866, and the other from degradative plasmid, pRA500 from P. putida NCIMB 9869, which harbor the structural genes for the flavoprotein (pchF) and cytochrome (pchC) subunits of p-cresol methylhydroxylase (PCMH), have been sequenced. The DNA and deduced amino acid sequences for pchC and pchF have been published. In these fragments, a coding region (dhal) for an aldehyde dehydrogenase has been identified. It is proposed that this gene encodes for the aldehyde dehydrogenase which converts p-hydroxybenzyaldehyde to p-hydroxybenzoate. p-Hydroxybezealdehyde is the product of oxidation of p-cresol by PCMH. The fragment from P. putida 9869 also harbors the genes for the ${\alpha}$ (pcaG) and ${\beta}$ (pcaH) subunits of protocatechuate 3,4-dioxigenase. The fragment from 9866 does not have any portion of these genes in the corresponding region A possible open reading frame (ORF) between pchC and pchF is seen for both clones, and a second putative open reading frame (ORF') also exists in the 9866 clone. The gene organizations are dhal-pchC-ORF-pchF-pcaGH for the DNA fragment from 9869, and ORF-dhal-pchC-ORF-pchF for the DNA fragment from 9866.

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THE AFFINITY OF CALMODULIN-AFFIGEL FOR INOSITOL TRIPHOSPHATE KINASE FROM BOVINE BRAIN (소의 뇌 Inositol triphosphate kinase와 Calmodulin-Affigel과의 친화도)

  • Lim, Sung-Woo;Kim, Jung-Hye
    • Journal of Yeungnam Medical Science
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    • v.7 no.1
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    • pp.39-50
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    • 1990
  • The one event on signalling mechanism is the cleavage by adenyl cyclase of ATP into second messenger, cyclic AMP. The other transfer system of inositol metabolism. it is widely recognized that hydrolysis of the minor membrane lipid phosphoinositide bisphosphate($PIP_2$) initiated by occupation of certain receptors and catalyzed by phospholipase C, lead to toe generation of the two intracellular messengers, inositol triphosphate($IP_3$) and diacylglycerol(DG). $IP_3$ is converted to inositol tetrakisphosphate($IP_4$) by $IP_3$ kinase. In the present study, it is that purification of calmodulin is used by phenyl-Sepharose CL-4B chromatography. it's molecular weigh, 17.000 in SDS-polyacrylamide gel electrophoresis. In order to observe the affinity between calmodulin (CaM)-Affigel 15 and $IP_3$ kinase, and isolated $IP_3$ kinase, was applied in CaM-Affigel with $Ca^{2+}$ equilibirum buffer and EGTA equilibirum buffer. We compared with binding and elution effect of $IP_3$ kinase in several condition of buffer. In affinity of binding. $Ca^{2+}$ equilibrium buffer was in the most proper condition. and elution, CaM/$Ca^{2+}$ buffer(CE1 10.36, CE2 12. 76pM/min/mg of protein) was effected much more than EGTA buffer(E2 1.48, E3 2.43pM/min/mg of protein), but CaM/$Ca^{2+}$ stimulate the activity of $IP_3$ kinase. And then, several detergents such as sodium deoxycholate, tween 20. cholic acid, polyethylene glycol, chaps were applied. The 0.2% chaps buffer(E2 23.19, E3 8.05pM/min/mg of protein) was the most effective in elution of $IP_3$ kinase.

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Change and Continuity in Regionalism: A Comparison of 1988, 2003, and 2016 Survey Results (지역주의의 변화: 1988년, 2003년 및 2016년 조사결과 비교)

  • Yoon, Kwang-Il
    • Korean Journal of Legislative Studies
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    • v.23 no.1
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    • pp.113-149
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    • 2017
  • This study aims to identify the micro-level, social psychological foundation of regionalism and analyze its change and continuity by comparing 1988, 2003, and 2016 survey results. Drawing on the theory of prejudice and social identity, it clarifies the concept of regionalism and examines its affective, behavioral, cognitive implications. In the empirical analysis, where it takes advantage of relevant questions of the same or similar wording in three nationally representative surveys, the study identifies the changes in regionalism at the individual level focusing on anti-Honam prejudice and discrimination and attribution of regional conflict. First, anti-Honam prejudice has been in decline nationally as well as regardless of where one has grown up, except for Daegu/Kyungpook area. Second, anti-Honam prejudice has been weakened among younger generations while regional party identification now affects the sentiment in the direction of regional cleavage overlapped with ideological leanings. Third, while most respondents do not experience explicit discrimination, Honam natives are still more likely to experience discrimination, especially identity and self-esteem related, due to his or her home town. Fourth, Honam natives have been more likely to attribute regional conflict to an external, structural factor like government economic policy and less likely to a subjective one like regional sentiment, which seems to be consistent with attributional attitudes of the victims of prejudice. The study ends with the discussion of how to reduce further anti-Honam prejudice, which includes contact hypothesis, recategorization, cross-categorization, and de-categorization.

Results of the 2019 European Parliament Elections: The Rise of Far-right Populism and Liberal Centrism (2019년 유럽의회선거의 함의: 극우 포퓰리즘과 중도자유주의의 부상)

  • OH, Chang Rhyong
    • Korean Journal of Legislative Studies
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    • v.25 no.2
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    • pp.5-33
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    • 2019
  • This study aims to explore the implications of the 2019 European Parliament elections. Existing studies show that European elections serve as secondary elections dominated by domestic issues. However, the 2019 European elections have highlighted pan-European issues such as the Brexit negotiations and the rise of far-right populism, with voter turnout also rising sharply to 50.62%, which is the highest record since the 2000s. There was also a significant change in the number of parliamentary seats held by each political group. First, the European People's Party(EPP) and the Progressive Alliance of Socialists and Democrats(S&D), which had hitherto led the European Parliament, failed to achieve a majority. Second, the number of seats won by Renew Europe, a new liberal-centrist group, was considerably high. Third, the far-right groups organized a full-fledged political force by rallying around the Identity and Democracy(ID) group. The rise of liberal-centrism can be explained as a reaction to the popularity of anti-European populism. Renew Europe's emphasis on open-market competition has created a different issue, especially since the center-left and center-right groups have stressed on the need for state intervention in employment, welfare, security and immigration control. Along with far-right populism, liberal centrism has also reshuffled Europe's political cleavage, and the conflict between 'liberalism' and 'protectionism' has become more evident in the European Parliament.

Evaluation for Rock Cleavage Using Distributional Characteristics of Microcracks and Brazilian Tensile Strengths (미세균열과 압열인장강도의 분포 특성을 이용한 결의 평가)

  • Park, Deok-Won
    • Korean Journal of Mineralogy and Petrology
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    • v.33 no.2
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    • pp.99-114
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    • 2020
  • The characteristics of the Brazilian tensile strengths(σt) parallel to the rock cleavages in Jurassic granite from Geochang were analysed. The evaluation for the six directions of rock cleavages was performed using the parameter values on microcrack length and the above strength. The strength values of the five test specimens belonging to each direction were classified into five groups. The strength values of these five groups increase in order of group A < B < C < D < E. The close dependence between the above microcrack and strength was derived. The analysis results of this study are summarized as follows. First, the chart showing the variation and characteristics of strength among the three rock cleavages were made. In the above chart, the strength values of six directions belonging to each group were arranged in order of rift(R1 and R2), grain(G1 and G2) and hardway(H1 and H2). The strength distribution lines of the five groups concentrate in the direction of R1. And the widths among the above five lines indicating strength difference(Δσt) are the most narrowest in R1 direction. From the related chart, the variation characteristics among the two directions forming each rock cleavage were derived. G2(2)-test specimen shows higher value and lower value of the difference in strength compared to the case of G1(1)-test specimen. These kinds of phenomena are the same as the case between the test specimen H2(2) and H1(1). The strength characteristics of the above test specimens (2) suggest lower microcrack density value and higher degree of uniformity in the distribution of microcracks arrayed parallel to the loading direction compared to those of test specimens (1). The six strength values belonging to each group were arranged in increasing order in the above chart. The strength values of the test specimens belonging to both group D and E appear in order of R1 < R2 < G1 < H1 < G2 < H2. Therefore, the strength values of group D and E can be indicator values for evaluating the six directions of rock cleavages. Second, the correlation chart between slope angle(θ) and strength difference(Δσt) were made. The values of the above two parameters were obtained from the five strength distribution lines connecting between the two directions. From the chart related to rift plane(G1-H1, R'), grain plane(R1-H2, G') and hardway plane(R2-G2, H'), the slope values of linear functions increase in order of R'(0.391) < G'(0.470) < H'(0.485). Among three planes, the charts related to hardway plane show the highest distribution density among the five groups. From the related chart for rift(R1-R2, R), grain(G1-G2, G) and hardway(H1-H2, H), the slope values of linear functions increase in order of rift(0.407) < hardway(0.453) < grain(0.460). Among three rock cleavages, the charts related to rift show the highest frequency of groups belonging to the lower region. Taken together, the width of distribution of the slope angle among the three planes and three rock cleavages increase in order of H' < G < R' < R < G' < H. Third, the correlation analysis among the parameters related to microcrack length and the tensile strengths was performed. These parameters may include frequency(N), total length(Lt), mean length(Lm), median length(Lmed) and density(ρ). The correlation charts among individual parameters on the above microcrack(X) and corresponding five levels of tensile strengths for the five groups(Y) were made. From the five kinds of correlation charts, the values of correlation coefficients(R2) increase along with the five levels of strengths. The mean values of the five correlation coefficients from each chart increase in order of 0.22(N) < 0.34(Lt) < 0.38(ρ) < 0.57(Lmed) < 0.58(Lm). Fourth, the correlation chart among the corresponding maximum strength for group E(X) and the above five parameters(Y) were made. From the related chart, the values of correlation coefficient increase in order of 0.61(N) < 0.81(Lt) < 0.87(ρ) < 0.93(Lm) < 0.96(Lmed). The two parameters that have the highest correlations are median length with maximum strength. Through the above correlation analysis between microcrack and strength, the credibility for the results from this study can be enhanced.

Anatomical Difference Between Two Rice Cultivars Selected to Oxyfluorfen (Oxyfluorfen에 내성(耐性) 및 감수성(感受性) 수도품종(水稻品種)에 대한 해부학적(解剖學的) 차이(差異))

  • Cheon, S.U.;Guh, J.O.;Lee, Y.M.;Lee, D.J.
    • Korean Journal of Weed Science
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    • v.8 no.2
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    • pp.187-198
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    • 1988
  • The second leaves from 30 days old seedlings of two rice cultivars which were selected as tolerant (cv. Chokoto) and susceptible (cv. Weld pally) cultivar were soaked in the concentration of $10^{-6}$, $10^{-5}$, $10^{-4}$ and $10^{-3}M$ of oxyfluorfen for 10, 15 and 22hrs and anatomical characteristics were abserved. Dipping to the solutions were carried out either directly to the attached leaves or to the seperated leaves. Development of any symptoms in epidermis, bundle sheath, mesophyll cells and bulliform cells were microscopically inspected. Both cultivars showed reductionin leaf thickness, but the susceptible ones was more sensitive than the tolerant. The degradation and disappearance of epidermal cell layer, breakage of bundle sheath cells, shrinkage of mesophyll cells and disappearance of bulliform cells were general response as affected by oxyfluorfen treatment. The susceptible cultivars showed such responses at the concentration of $10^{-5}M$ for 10 hrs while tolerant ones $10^{-3}M$, for 10 hrs. Those treatments were more effective in seperated leaves than in attached ones. The epicuticular wax layer of leaves treated as above for 20 hrs was inspected by SEM. Weld pally, the susceptible cultivar (Weld pally) showed rapid cleavage of wax layer under $10^{-5}M$ concentration while the tolerant (Chokoto) showed only minor damage on wax layer at the concentration of $10^{-3}M$.

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Quaternary Geology and Paleoecology of Hominid Occupation of Imjin Basin (임진강유역 구석기 공작의 고생태학적 배경)

  • Seonbok Yi
    • The Korean Journal of Quaternary Research
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    • v.2 no.1
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    • pp.25-50
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    • 1988
  • The survival of rich evidence of palaeolithic occupation found in the Imjin-Hant'an River basin was possible due to many fortuitous geological conditions provided there. Formation of the basalt plain in a narrow valley system which developed during the late Mesozoic insured the appearance of a basin of sedimentation in which archaeological sites would be preserved with relatively minor post-depositional disturbance. Geomagnetic and K-Ar dating indicates that lava flows occurred during the Brunes Normal Epoch. During and after the process of basin sedimentation, erosion of the plain was confined to the major channel of the present river system which developed along the structural joints formed by the lava flow. Due to characteristic columnar structure and platy cleavage of the basalt bedrock, erosion of the basalt bedrock occurred mainly in vertical direction, developing deep but narrow entrenched valleys cut into the bedrock. Consequently, the large portion of the site area remained intact. Cultural deposits formed on top of the basalt plain were left unmodified by later fluvial disturbances due to changes in the Hant'an River base-level, since they were formed about 20 to 40m above the modern floodplain. Sedimentological evidence of cultural deposits and palynological analysis of lacustrine bed formed in the tributary basin of the Hant'an River indicate that hominid occupation occurred in this basin under rapidly deteriorating climatic conditions. From three thermoluminescence dates, the timing of hominid occupation as represented by 'Acheulian-like' bifaces apparently occur sometime during 45,000 BP. Thus, deposition of cultural layers in this basin approximately coincides with the beginning of the second stadial of the final glacial, during which the Korean Peninsula must have had provided a sanctuary for prolonged human occupation.

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