• Title/Summary/Keyword: SecY

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Utilization of Valley Water for River Maintenance Discharge (계곡수를 활용한 하천유지용수 확보 방안에 대한 연구)

  • Park, Chang-Eon;Kim, Je-Hyeong
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1022-1026
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    • 2006
  • 성남시 남한산성 일부 유역을 대상으로 하여, 하수처리시설이 잘 갖추어진 도시하천의 하천유지용수 확보를 위한 방안으로 유역의 계곡수를 활용하는 방안에 대하여 검토하였다. 4개소의 계곡수 유출 지점에 유량계를 설치하여 일정 기간 동안의 유량을 관측하였으며, 이를 바탕으로 각 지점의 유출특성을 분석하여 연중 계곡수의 수량을 분석하였는데, 강우가 1mm 이하로 발생되어진 319일 동안 계곡수를 양수하여 인근의 건천화된 창곡천과 야탑천으로 통수한다면 갈수 시 유량이 각각 $0.034m^3/sec,\;0.050m^3/sec$였던 하천에 $0.174m^3/sec,\;0.450m^3/sec$의 유량을 유지할 수 있게 하여 건천화 해소에 크게 기여할 수 있는 것으로 나타났다. 더구나, 이는 분류식 하수처리체계를 가지고 있는 해당 지역의 특성으로 인하여 하수처리장으로 유입되는 계곡수의 유량만큼을 처리용량에서 감소시킬 수 있어 하수처리장의 운영효율 제고 및 유지관리비 절약에도 기여할 수 있을 것으로 판단되었다.

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Intragenic Suppressors for Expory-defective Signal Sequence Mutation of Ribose-binding Protein in Escherichia coli (대장균 리보스 결합단백질의 신호배열 변이에 대한 숙성체 부위의 회복돌연변이)

  • 이영희;송택선;김정호;박순희;박찬규
    • Korean Journal of Microbiology
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    • v.29 no.5
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    • pp.270-277
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    • 1991
  • A mutational alteration in the signal sequence of ribose-binding protein (RBP) of Escherichia coli, rbsB103, completely blocks the export of the protein to the periplasm. Intragenic suppressors for this mutation have been selected on minimal medium with ribose as a sole carbon source. Six suppressor mutations were characterized in detail and were found to have single amino acid wubstitution in the mature portion of RBP, which resulted in the mobility shift of the proteins on SDS polyacrylamide gel. Amino acid changes of these suppressors were localized in several peptides which are packed to form the N terminal domain of typical bilobate conformation of RBP. The involvement of SecB, a molecular chaperone, was investigated in the suppression of signal sequence mutation. Translocation efficency was found to be increased by the presence of SecB for all suppressors. It is likely that the folding characteristics of RBP altered by the suppressor mutations affect the affinity of interaction between SecB and RBP.

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High Temperature Size Exclusion Chromatography for High Throughput Analysis

  • Chang, Tai-Hyun;Park, Soo-Jin;Cho, Hee-Sook;Kim, Young-Tak;Ihm, Kyu-Hyun
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.202-202
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    • 2006
  • With a modern size exclusion chromatography (SEC) column, molecular weight analysis of a polymer sample takes about 10 min. However, it is desirable to reduce the analysis time further, in particular, for high throughput measurements required in combinatorial analyses or 2D-HPLC analyses. We implemented the high temperature SEC for the purpose. By inserting a narrow bore tubing between the column and the detector, a sufficient backpressure can be maintained to prevent the mobile phase from boiling and the effluent is cooled down enough when it reaches the detector. Therefore, a normal SEC detector can be used without any modification. The SEC resolution is greatly improved at the elevated temperature at high flow rate which allows high speed operation.

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Removal of toluene using the 2-stage hollow fiber membrane-hybrid reactor (중공사막을 적용한 2단 멤브레인 하이브리드 반응기에 의한 톨루엔 제거)

  • Kim, Jin-Sung;Gu, So-Hee;Kim, Tae-Hyeong;Lee, Myoung-Joo;Hwang, Sun-Jin
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.3
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    • pp.287-293
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    • 2010
  • In this study, the toluene gas in VOCs was removed using bioreactor which applied with hollow fiber membrane and Pseudomonas sp. TDB-4. The EBRT of each reactor are controlled 60 sec(R-1) and 30 sec(R-2) and inlet tolune concentration of both R-1 and R-2 is controlled from 25ppm to 500 ppm. Up to 500 ppm of toluene concentration, the toluene removal efficiency of R-1 and R-2 are 92% and 81%, and theirs removal capacities are about 100 g/$m^3$/hr and 180 g/$m^3$/hr, respectively. In addition, according to this study, toluene removal efficiencies at the hollow fiber are approximately 70%(60 sec) and 45%(30sec).

The Analysis on Flow Characteristics of Increasing Velocity by Accumulated Debris at the Pier (교각주변에 부유잡목 집적시 유속증가에 따른 흐름특성분석)

  • Choi, Gye-Woon;Gwon, Yong-Hyeon;Kim, Young-Kyu;Kim, Joo-Hwan
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.2202-2206
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    • 2008
  • 본 연구에서는 물의 다양한 흐름으로 인한 교각주변의 수위 및 유속변화에 대하여 연구하기 위해 수리모형을 설치하여 개도비를 70%로 고정시키고 유속을 44cm/sec, 57cm/sec, 72cm/sec, 84cm/sec로 변화시켜 부유잡목의 높이와 폭에 따른 흐름특성을 분석하였다. 그 결과 동일개도비 70%상에서 상류에서는 유속이 증가하면서 수위 또한 증가를 하였으며 교각 직상류에서는 갑자기 증가하였다. 교각 직하류부에서는 수위가 감소하여 최저 수위가 나타났으며 수위변화가 매우 불규칙하게 나타났다. 또한 교각설치 직상류부와 하류부에서는 수위차가 크게 나타났으며 유속이 증가 할수록 수위차는 더욱 크게 나타났다. 동일개도비의 부유잡목 중 폭보다 깊이 방향으로의 크기가 증가하고 유속이 증가함에 따라 교량 하류부의 유속이 매우 크게 증가하여 교량피해에 큰 영향을 줄 것으로 판단되며 교각의 하류부 또한 매우 불규칙한 유황으로 인한 피해도 우려된다.

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A study on the rheological properties of superfluidity self compacting concrete utilizing tailings from the tungsten mine (광산광미를 활용한 초유동 자기충전 콘크리트의 유변학적 특성 연구)

  • Choi, Yun-Wang;Kim, Yong-Jic;Choi, Wook;Lee, Kwang-Myong
    • Proceedings of the Korea Concrete Institute Conference
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    • 2006.05b
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    • pp.89-92
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    • 2006
  • This study has focused on the possibility for recycling of tailings from the Sangdong tungsten mine as powder of superfluidity self-compacting concrete. The experimental tests for slump-flow, time required to reach 500mm of slump flow(sec), time required to flow through V-funnel(sec) and filling height of U-box test(mm) were carried out in accordance with the specified by the Japanese Society of Civil Engineering(JSCE). The result of this study, in case of superfluidity self-compacting concrete mixed with tailings, slump-flow was decreased with increasing mixing ratio. But time required to reach 500mm of slump flow(sec), time required to flow through V-funnel(sec) and filling height of U-box test(mm) were satisfied a prescribed range.

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MgO Properties Depending on E-beam Evaporation Rate and Its Effects on the PDP Discharging Characteristics

  • Kwon, Sang-Jik;Kim, Yong-Jae;Li, Zhao Hui;Kim, Kwang-Ho;Lee, Dal-Ho
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.890-893
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    • 2006
  • Effects of the evaporation rate of MgO films using electron beam were investigated on the MgO properties and the discharge characteristics of the plasma display panel (PDP). The evaporation rate was changed from 3 ${\AA}/sec$ to 15 ${\AA}/sec$ at a substrate temperature of $300\;^{\circ}C$. MgO properties such as crystal orientation, surface roughness, contact angle, and film structure were inspected using XPS, AFM, drop shape analysis and SEM. We also studied the relation between MgO properties and PDP discharging characteristics. The minimum firing voltage and maximum efficacy were obtained at evaporation rate of 5 ${\AA}/sec$. In the MgO film deposited at 5 ${\AA}/sec$, (200) orientation was most intensive and surface roughness was minimum.

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DEVELOPMENT OF A HIGH SPEED CCD CAMERA SYSTEM FOR THE OBSERVATION OF SOLAR Ha FLARES

  • VERMA V. K.;UDDIN WAHAB;GAUR V. P.
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.391-392
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    • 1996
  • We have developed and tested a CCD camera (100 $\times$ 100 pixels) system for observing Ha images of the solar flares with time resolution> 25 msec. The 512 $\times$ 512 pixels image of CCD camera at 2 Mpixels/sec can be recorded at the rate of more than 5 frame/sec while 100 $\times$ 100 pixels area image can be obtained 40 frames/sec. The 100 $\times$ 100 pixels image of CCD camera corresponds to 130 $\times$ 130 arc - $sec^2$ of the solar disk.

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A Study on the Reduction Effect of Vibration of NPS Blasting Method (NPS 발파공법의 진동감쇠효과에 관한 연구)

  • Sim, Dong-Soo;Lee, Shin;Kang, Dae-Woo
    • Explosives and Blasting
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    • v.21 no.1
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    • pp.59-68
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    • 2003
  • 선 균열을 위한 기존의 PS(presplitting)발파공법은 암반사면의 안정에 주로 활용되어져 왔으나, 진동 및 폭음이 크며 절단효과의 미약 등 적용상의 한계로 인하여 여러 가지 문제가 제기되어져왔다. 이러한 한계를 보완하여, 최근에 개발된 NPS(new presplitting)발파공법은 시공상의 진동 및 폭음공해가 사실상 없을 뿐만 아니라 절단효과도 탁월하고, 진동감쇠기능도 우수하여 방진공법으로서 활용가치가 크게 부각되고 있다. 다라서, 방진공법으로서의 NPS 발파공법의 진동감쇠효과를 연구하고자 했다. 그 결과 본 지역에서는 NPS 발파공법 실시전의 지반진동 크기는 1.45cm/sec ~ 0.14cm/sec 이었으나 4열의 NPS 발파실시 후 지반지동 크기는 0.72cm/sec ~ 0.11cm/sec로 나타나 평균 57%의 진동감쇠율을 보였다.