• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1070-1076
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• 1998

The processing conditions of low salt mackerel (Scomber japonicus) fillet was investigated, in which fresh mackerel was filleted, salted in brine until the expected salt concentration reached, dried with cool air (3 m/sec, $10{\sim}20^{\circ}C$), and finally packed individually in polyvinyl chloride film. Salting time and salt concentration of brine decided the final salt level penetrated into the fillet. As the final salt level was fixed to $0.8{\sim}1.0%, salting for $15{\sim}20 hours with 5% or 10% brine at $5^{\circ}C$ was enough to get that level of salt. Formation of histamine during salting was negligible. Changes in VBN, salt soluble proteins, and histamine formation of salted mackerel fillet during the storage occurred more rapidly in cases of storage at $5^{\circ}C than af $-2^{\circ}C and $-20^{\circ}C. Oxidation of lipid during the storage progressed, however it was delayed longer then 100 days in case of storage at $-20^{\circ}C. Addition of sodium erythrobate or ginger extracts could provide some extent of browning retardation. The shelf-life of the salted mackerel fillet based on panel scores of brown color and rancidity appealed to be 14 days when stored at $5^{\circ}C, and more than 28 days in case of storage at $-2^{\circ}C and about 3 months stored at $-20^{\circ}C.

• Journal of Embryo Transfer
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• v.10 no.3
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• pp.209-217
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• 1995

The present study was carried out to develop a cloning technology of mouse embryos by nuclear transplantation with electrofusion and to produce cloned offsprings by transfer of reconstituted embryos. A single nucleus from two- and eight-cell embryos was transplanted into the enucleated two-cell embryos by rnicromanipulation. The fusion of nucleus with recipient cytoplasm and the subsequent development of reconstituted embryos in vitro as well as in vivo to term were examined to determine the optimal electrofusion parameters for nuclear transplantation in mouse embryos. The successful enucleation of donor embryos was 84.9 and 83.3% in two- and eight-cell stage, respectively, and the successful injection of nucleus from two- and eight-cell donor embryos into the perivitelline space of enucleated two-cell embryos were 85.1 and 84.7%, respectively. No significant differences were found in enucleation or injection rate between the cell stages of donor embryos. When the blastomeres of intact two-cell mouse embryos were electrofused in 0.3 M mannitol medium(100 $\mu$sec., 3 pulses), the fusion rate was similarly 93.2, 92.2 and 92.0% in 1.0, 1.5 and 2.0 kV /crn, respectively, but in vitro development to blastocyst of the fused two-cell embryos was significantly(P<0.05) lower in 2.0 kV/cm (63.4%) than in 1.0 kV/cm (91.7%) or 1.5 kV/cm (82.4%). The development in vitro to eight-cell stage of the reconstituted embryos with nucleus from two-cell stage(45.5%) was significantly(P<0.05) higher than that from eight-cell stage blastomeres (16.7%). The number of blastomeres of the intact embryos at blastocyst stage was 50i0.6 and 55$\pm$2.4 in in vitro and in vivo cultured mouse embryos, respectively, but significantly(P<0.05) decreased to 35$\pm$0.7 in nuclear transplanted blastocyst embryos. The conception rate of mice following embryo transfer was 32.1% in the reconstituted two-cell embryos using two-cell donor nuclei, which was comparable to the fresh two-cell embryos(40.6%). However, the rate of development in vivo to term following embryo transfer of the reconstituted two-cell embryos using two-cell donor nuclei (23.5%) was significantly(P<0.05) lower compared with the percentage of two-cell fresh embryos(31.5%).

• 한국해양학회지
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• v.22 no.3
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• pp.143-152
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• 1987

The digital data were obtained using Kennedy 9000 magnetic tape deck which was connected to the SMS960 side scan sonar during the field operations. The data of three consecutive survey tracks near Seongsan-po, Cheju were used for the development of this study. The softwares were mainly written in Fortran-77 using VAX 11/780 MINI-COMPUTER (CPU Memory; 4MB). The established mapping system consists of the pretreatment and the digital processing of seafloor image data. The pretreatment was necessary because the raw digital data format of the field magnetic tapes was not compatible to the VAX system. Therefore the raw data were read by the personal computer using the Assembler language and the data format was converted to IBM compatible, and next data were communicated to the VAX system. The digital processing includes geometrical correction for slant range, statistical analysis and cartography of the seafloor image. The sound speed in the water column was assumed 1,500 m/sec for the slant range correction and the moving average method was used for the signal trace smoothing. Histograms and cumulative curves were established for the statistical analysis, that was purposed to classify the backscattering strength from the sea-bottom. The seafloor image was displayed on the color screen of the TEKTRONIX 4113B terminal. According to the brief interpretation of the result image map, rocky and sedimentary bottoms were very well discriminated. Also it was shown that the backscattered acoustic pressurecorrelateswith the grain size and sorting of surface sediments.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.478-478
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• 2012

Recently, there are many researches in order to increase the deposition rate (D/R) and improve film uniformity and quality in the deposition of microcrystalline silicon thin film. These two factors are the most important issues in the fabrication of the thin film solar cell, and for the purpose of that, several process conditions, including the large area electrode (more than 1.1 X 1.3 (m2)), higher pressure (1 ~ 10 (Torr)), and very high frequency regime (VHF, 40 ~ 100 (MHz)), have been needed. But, in the case of large-area capacitively coupled discharges (CCP) driven at frequencies higher than the usual RF (13.56 (MHz)) frequency, the standing wave and skin effects should be the critical problems for obtaining the good plasma uniformity, and the ion damage on the thin film layer due to the high voltage between the substrate and the bulk plasma might cause the defects which degrade the film quality. In this study, we will propose the new concept of the large-area multi-electrode (a new multi-electrode concept for the large-area plasma source), which consists of a series of electrodes and grounds arranged by turns. The experimental results with this new electrode showed the processing performances of high D/R (1 ~ 2 (nm/sec)), controllable crystallinity (~70% and controllable), and good uniformity (less than 10%) at the conditions of the relatively high frequency of 40 MHz in the large-area electrode of 280 X 540 mm2. And, we also observed the SEM images of the deposited thin film at the conditions of peeling, normal microcrystalline, and powder formation, and discussed the mechanisms of the crystal formation and voids generation in the film in order to try the enhancement of the film quality compared to the cases of normal VHF capacitive discharges. Also, we will discuss the relation between the processing parameters (including gap length between electrode and substrate, operating pressure) and the processing results (D/R and crystallinity) with the process condition map for ${\mu}c$-Si:H formation at a fixed input power and gas flow rate. Finally, we will discuss the potential of the multi-electrode of the 3.5G-class large-area plasma processing (650 X 550 (mm2) to the possibility of the expansion of the new electrode concept to 8G class large-area plasma processing and the additional issues in order to improve the process efficiency.

• The Korean Journal of Pain
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• v.12 no.1
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• pp.36-42
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• 1999

Background: The current maximal recommended doses of lidocaine are 7 mg/kg with $5\;{\mu}g/ml$ of epinephrine. But in clinical practice, sometimes more doses of lidocaine are required to produce adequate regional anesthesia. Method: Twenty-two healthy women patients were divided into two groups and pretreated with valium 5 mg p.o., morphine 5 mg i.m., and midazolam 2 mg i.v. before operation. Of these, 7 mg/kg of 2% lidocaine with $5\;{\mu}g/ml$ of epinephrine were given to 11 patients epidurally. Initial 3 ml of epinephrine mixed lidocaine was given as a test dose and remaining doses were given 5 ml/30 sec with 3 min intervals. Radial arterial blood were drawn at 5, 10, 15, 20, 30, 45, 60, 90, 120 min to measure plasma lidocaine concentrations. After confirming all of the peak plasma concentrations of 7 mg/kg lidocaine were absolutely under $5\;{\mu}g/ml$, the other 11 patients were given 10 mg/kg of 2% lidocaine with $5\;{\mu}g/ml$ of epinephrine epidurally and blood samplings were taken according to the same method of 7 mg/kg group. The peak plasma concentration ($C_{max}$), time to reach to $C_{max}$ ($T_{max}$), time to reach to $T_4$, maximal sensory block level, systemic toxicity, and vital sign changes were investigated. Result: $C_{max}$ was significantly higher in 10 mg/kg group ($5.1{\pm}1.3\;{\mu}g/ml$) than 7 mg/kg group($3.3{\pm}0.5\;{\mu}g/ml$), but $T_{max}$ ($10.5{\pm}2.7$ min vs $10.9{\pm}3.1$ min) was not different. Time to reach $T_4$ was significantly shorter in 10 mg/kg group ($9.5{\pm}2.7$ min) than 7 mg/kg group ($12.7{\pm}3.2$ min) but maximal sensory block level ($T_{3.7{\pm}0.7}$ vs $T_{2.7{\pm}1.0}$) was not different. In four patients of 10 mg/kg group, peak plasma concentrations exceeded $5\;{\mu}g/ml$, but no systemic toxicities appeared. No significant vital sign changes were observed. Conclusion: The current maximal recommended doses of lidocaine, merely based on body weight are not always appropriate. Further studies are needed to determine more precise guideline of maximal doses that include various pharmacokinetic components.

• Journal of Biomedical Engineering Research
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• v.19 no.3
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• pp.251-260
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• 1998

The Holter monitoring system is a widely used noninvasive diagnostic tool for ambulatory patient who may be at risk from latent life-threatening cardiac abnormalities. In this paper, we design a high performance intelligent holter monitoring system which is characterized by the small-sized and the low-power consumption. The system hardware consists of one-chip microcontroller(68HC11E9), ECG preprocessing circuit, and flash memory card. ECG preprocessing circuit is made of ECG preamplifier with gain of 250, 500 and 1000, the bandpass filter with bandwidth of 0.05-100Hz, the auto-balancing circuit and the saturation-calibrating circuit to eliminate baseline wandering, ECG signal sampled at 240 samples/sec is converted to the digital signal. We use a linear recursive filter and preprocessing algorithm to detect the ECG parameters which are QRS complex, and Q-R-T points, ST-level, HR, QT interval. The long-term acquired ECG signals and diagnostic parameters are compressed by the MFan(Modified Fan) and the delta modulation method. To easily interface with the PC based analyzer program which is operated in DOS and Windows, the compressed data, that are compatible to FFS(flash file system) format, are stored at the flash memory card with SBF(symmetric block format).

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.2
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• pp.350-361
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• 1996

The objectives of this study mere 1) to investigate whether the different nonwoven fabric types influenced on the objective and subjective wear performances of the experimental pesticide protective pants, 2) to detect whether the different finishes treated to the nonwoven fabrics influenced on the objective and subjective wear performances of the experimental pants, and 3) to detect the relationships between objective wear performances and subjective wear sensation. Three types of nonwoven fabrics (T (Tyvek$\textregistered$), 5 (Sontara$\textregistered$) and K (Kimlon$\textregistered$)) were used as test specimens. By pad-dry.cure method, each of the specimen was treated with fluorocarbon compound for water-oil repellent finish (Tw, Sw, Kw). And each of specimen was treated with organic silicon quarternary ammonium salts and then treated with fluorocabon compound for dual functional finish (76, 50, Kd). Using the three water-oil repellent finished fabrics and the three dual functional finished fabrics, six experimental protective pants (Cl (Tw), C2 (Sw), C3 (Kw), C4 (76), C5 (56), C6 (Kd)) were made according to the same pattern suggested by the Rual Guidance Office. The wear trials of experimental pesticide protective pants were performed in a conditioned vinyl plastic hothouse ($30\pm1^{\circ}C$, $70\pm5%$R.H., 0.25m/sec air velocity). The measurements of skin temperature, microclimate temperature and humidity on the subjects were obtained by the themohygromenter. The subjective wear sensations were measured using previously developed thermal, humidity and overall comfort scales. The results obtained from this study were as follows: 1) There were siginificant differences among nonwoven fabric types on the objective and subjective wear performances, therefore, the skin temperature, microclimate temperature and humidity of subjects who wore the experimental pants made with Sontara were siginificantly lower than those who wore the others. And, the experimental pants made with Sontara were assessed as more comfortable than the others in terms of the subjective thermal, humidity and overall wear sensations. 2) There were no significant differences between two finish types on the objective and subjective wear Performances. 3) The microclimate humidity on the thigh was highly correlated with the overall subjective comfort sensations and the next highly correlated one was the mean skin temperature. That is, the higher the microclimate humidity and the mean skin temperature, the higher the overall subjective comfort sensation ratings which mean the overall subjective sensation was very uncomfortable.

• Korean Journal of Food Science and Technology
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• v.47 no.3
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• pp.293-298
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• 2015

This study aimed at characterizing the rheological properties and molecular weight of tara gum fractionated with ammonium sulfate. Tara gum was separated into six fractions (F1-F6) at different concentrations of ammonium sulfate, ranging from 12.21 to 28.67% (w/w). The yield of the tara gum fractions ranged between 4.98 and 17.47%, and their intrinsic viscosity ranged from 9.38 to 12.44 dL/g. The highest values of Huggins coefficient (k') and viscosity-molecular mass were observed in fraction F3. The shear viscosity of the tara gum fractions was measured by a cone-plate viscometer, clearly showing shear thinning behavior. Size-exclusion chromatography results showed that the molecular weight ranged between 635.42 and 776.71 kg/mol, and the F3 fraction exhibited higher values of molecular weight.

• Journal of dental hygiene science
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• v.15 no.3
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• pp.254-258
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• 2015

To compare the stationary dental X-ray generator and the portable dental X-ray generator and to understand spatial radiation dose depended on locations by measuring spatial radiation dose of the portable dental X-ray generator. The researchers used an Ionization chamber to measure spatial radiation dose which was generated while applying X-ray radiation to real bone skull phantom with both portable and stationary dental X-ray generator. There were 4 measurement locations which were immediate anterior, right, left and posterior. Distance of measurement was 50 cm in every location and the recorded result is an average of two applications of X-ray radiation to the maxillary molar area under the condition of 70 kVp, 3 mA, 0.1 sec. Average spatial radiation dose of portable X-ray generator was $37.51{\mu}Sv$, much higher than that of stationary X-ray generator which was $10.77{\mu}Sv$ (p<0.001). The result of the spatial radiation dose of the portable X-ray generator showed a huge difference depending on types of units which varied from $17.77{\mu}Sv$ to $68.90{\mu}Sv$ (p<0.05), also depending on the measurement location, immediate anterior resulted in the highest radiation dose of $54.14{\mu}Sv$ and immediate right was the lowest of $13.60{\mu}Sv$. Immediate left and posterior, however, resulted in similar radiation dose which were $42.12{\mu}Sv$, $40.18{\mu}Sv$ (p<0.01). With this result, we claim that usage of portable dental X-ray generator should be restricted to patients who can't move and exposure to radiation should be minimized by wearing lead-apron.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.1-8
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• 2006

The purpose of this study was to evaluate normalized jerk according to shoes, slope, and velocity during walking. Eleven different test subjects used three different types of shoes (running shoes, mountain climbing boots, and elevated forefoot walking shoes) at various walking speeds(1.19, 1.25, 1.33, 1.56, 1.78, 1.9, 2, 2.11, 2.33m/sec) and gradients(0, 3, 6, 10 degrees) on a treadmill. Since there were concerns about using the elevated forefoot shoes on an incline, these shoes were not used on a gradient. Motion Analysis (Motion Analysis Corp. Santa Rosa, CA USA) was conducted with four Falcon high speed digital motion capture cameras. Utilizing the maximum smoothness theory, it was hypothesized that there would be differences in jerk according to shoe type, velocity, and slope. Furthermore, it was assumed that running shoes would have the lowest values for normalized jerk because subjects were most accustomed to wearing these shoes. The results demonstrated that elevated forefoot walking shoes had lowest value for normalized jerk at heel. In contrast, elevated forefoot walking shoes had greater normalized jerk at the center of mass at most walking speeds. For most gradients and walking speeds, hiking boots had smaller medio-lateral directional normalized jerk at ankle than running shoes. These results alluded to an inverse ratio for jerk at the heel and at the COM for all types of shoes. Furthermore, as velocity increased, medio-lateral jerk was reduced for all gradients in both hiking boots and running shoes. Due to the fragility of the ankle joint, elevated forefoot walking shoes could be recommended for walking on flat surfaces because they minimize instability at the heel. Although the elevated forefoot walking shoes have the highest levels of jerk at the COM, the structure of the pelvis and spine allows for greater compensatory movement than the ankle. This movement at the COM might even have a beneficial effect of activating the muscles in the back and abdomen more than other shoes. On inclines hiking boots would be recommended over running shoes because hiking boots demonstrated more medio-lateral stability on a gradient than running shoes. These results also demonstrate the usefulness of normalized jerk theory in analyzing the relationship between the body and shoes, walking velocity, and movement up a slope.