• Ocean and Polar Research
• /
• 제31권4호
• /
• pp.319-326
• /
• 2009

Analysis of four microsatellite markers from Mud Crab Scylla serrata revealed that there is high level of genetic diversity within this species. Genetic diversity of S. serrata was calculated using allele diversity, observed heterozygosity, expected heterozygosity (Het-exp), polymorphic information content, gene differentiation and Nei's $D_{A}$ distance. Mean polymorphic information content value was 0.797, which reflected high level of polymorphism across the loci of S. serrata. The Palau population has the highest genetic diversity (Het-exp=0.871), while the Kosrae population has the lowest genetic diversity (Hetexp=0.806). However, the geographical genetic distance among S. serrata populations from Yab, Chuuk, Pohnpei, Kosrae, and Palau were low (0.2009${\sim}$0.3350). These results suggest that despite their wide distribution, S. serrata are no different in geographical genetic diversity within the five sampled locations.

• 한국미생물·생명공학회지
• /
• 제49권3호
• /
• pp.432-439
• /
• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• Ocean and Polar Research
• /
• 제31권3호
• /
• pp.257-264
• /
• 2009

Seventeen females of the mud crab Scylla serrata, from the State of Kosrae, Micronesia, were transported to the Fisheries Resources Research Institute, Gyeongsangnam-do, in oxygen-filled plastic bags. After acclimatization to a $30^{\circ}C$ holding temperature, nine females were selected for seed production trials. Spawning was hastened using eyestalk ablations; however, this may not be required in commercialscale mud crab seed production. Primary spawning produced an average of 2.4 million hatched larvae, whereas secondary spawning produced 0.4 million. About 10 days elapsed between spawning and hatching and 30 days between hatching and crablet. Mass mortalities up to 90% were observed between stages zoea 1 and zoea 2 in every trial. The highest survival rate from zoea 1 to crablet was estimated at 0.25%. Most commercial shrimp hatcheries in Korea are equipped with almost all necessary facilities and could be converted easily to mud crab hatcheries, able to run three to four times per year using hatchery technologies developed for blue crabs and Chinese mitten crabs.

• 수산해양기술연구
• /
• 제14권1호
• /
• pp.15-36
• /
• 1978

몇 가지 어류와 갑각류의 식이음과 울음소리를 분석하고, 그 소리를 방음할 때의 주음반응을 조사 연구하였다. 1. 어류의 식이음의 주파수는 참돔(Chrysophrys major)과 말쥐치(Navodon modestus)는 63~125Hz로서 낮고, 까치복(Fugu xanthopterus)은 400~500Hz로서 높으며, 소조기(Nibea albiflora)와 방어(Seriola quinqueradiata)는 그들의 중간인 125~250Hz이였다. 말쥐치와 참돔의 식이음과 음압은 55~59db로서 낮고, 까치복은 60~64db로서 높으며, 소조기와 방어는 57~62db로 이들의 중간이었다. 2. 수조기의 울음소리의 주파수는 125~250Hz로서 식이음과 같으나, 음압준위는 62~63db로서 식이음보다 높았다. 3. 갑각류의 울음소리의 주파수는 왕게(Scylla serrata)가 125~250Hz로서 낮고, 민꽃게(Charybdis japonica)와 왕밤송이게(Telmessus acutidens)가 500~1,000Hz로서 높으며, 꽃게(Portunus trituberculatus)는 250~500Hz로서 그들의 중간이었다. 음압준위는 왕게가 68~70db로서 높고, 꽃게, 민꽃게와 왕밤송이게는 54~61db로써 낮았다. 4. 수조기와 방어의 식이음에 대한 주음률은 56~87%였으며, 말쥐치와 까치복은 주음성을 확인하기 어려웠다. 수조기의 울음소리에 대한 주음률은 52~63%였다. 5. 수조기, 방어, 까치복, 말쥐치, 참돔 등은 주파수가 50~9,000Hz인 정현파에는 주음하지 않았다. 6. 꽃게의 울음소리에 대한 주음은 대형군(갑폭 15.1~18.5cm)이 방음할 때, 소형군(8.5~12.5cm)의 주음률(13~58%)보다는 소형군이 방음할 때 대형군의 주음률(44~98%)이 높았다. 중소형군(각폭 8.5~15.0cm)까지는 암컷이 방음할 때 수컷의 주음률(88~100%)은 암컷의 주음률(56~91%)보다 높은 편이며, 수컷이 방음할 때 수컷의 주음률(19~63%)보다 암컷의 주음률(66~100%)이 높은 편이었다.