• 한국식품영양과학회지
• /
• 제35권5호
• /
• pp.543-548
• /
• 2006

본 실험은 황금열수추출물이 알레르기성 접촉 피부염 유발 시 항산화활성, serum Ig E농도와 표피회복능에 미치는 영향을 조사하기 위해 행해진 것으로 1-chloro-2,4-dinitrochlorobenzene(DNCB: 2.5% DNCB 1 mL로 감작, 4일 경과 후 1% DNCB 1 mL로 유발) 처리로 알레르기성 접촉 피부염이 유발된 Sprague Dawley rat 수컷 흰쥐에 황금열수추출물(1 mL/kg/day)을 경구투여한 다음 혈장과 간의 항산화활성, 혈중 Ig E 농도, 표피두께변화를 관찰하였다. DNCB 처리에 의해 지질과산화물 함량과 Ig E 농도, 표피두께가 증가하였으며 황금열수추출물의 경구투여에 의해 간의 지질과산화물 함량 감소, 혈중 Ig E 농도 감소, 표피두께가 저하되는 결과를 보였다. 이상의 결과로 미루어 황금열수추출물이 알레르기성 접촉피부염으로 유발된 rat의 체내 항산화활성과 Ig E 농도개선, 표피두께 개선에 효과가 있는 것으로 생각된다.

• Korean Journal of Acupuncture
• /
• 제26권3호
• /
• pp.55-68
• /
• 2009

Objectives : To investigate the anti-inflammatory effects of Scutellaria baicalensis Georgi pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+Scutellaria baicalensis Georgi pharmacopuncture at BL23 (n=6, BL23), LPS+Scutellaria baicalensis Georgi pharmacopuncture at CV12 (n=6, CV12), and LPS+Scutellaria baicalensis Georgi pharmacopuncture at GV4 (n=6, GV4). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by intraperitoneal LPS injection (5mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results : For proinflammatory cytokines, CV12 pharmacopuncture group was significantly different compared with the control group in plasma IL-$1{\beta}$, IL-6, TNF-$\alpha$, and IL-10 5 h after LPS injection (P<0.05). For plasma IL-$1{\beta}$ and IL-6, CV12 pharmacopuncture group also showed significant difference at 2 h compared with the control (P<0.05). GV4 pharmacopuncture group was significantly different compared with the control at 5 h in plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ and at 2 h in IL-10 (P<0.05). Liver cytokines were analyzed at 5 h after LPS injection; only CV12 pharmacopuncture group showed significant difference in IL-$1{\beta}$ (P<0.05) and others including IL-6, TNF-$\alpha$, and IL-10 had no difference compared with the control group. CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). Plasma NO3-/NO2- and intracellular adhesion molecule-1 of CV12 pharmacopuncture group were significantly lower than that of the control group (P<0.05). In the plasma concentration of prostaglandin E2, all 3 pharmacopuncture groups had significantly lower values than that of the control group (P<0.05), but there was no difference among pharmacopucnture groups. Monocyte chemoattractant protein-1 and cytokine-induced neutorphil chemoattractant-1 in peritoneal lavage fluid was significantly decreased in CV12 pharmacopuncture group compared with the control group (P<0.05). Conclusions : These results indicate that Scutellaria baicalensis Georgi pharmacopuncture at CV12 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

• 대한임상검사과학회지
• /
• 제50권4호
• /
• pp.438-443
• /
• 2018

주술적 의료로 이용되는 식물에서 새로운 항미생물질을 찾는 있는 중에 바이오필름의 출현은 임상, 의학 제조업 분야에서 다급한 신호를 보내주고 있는 것이다. 국립보건원(NIH)은 세계 모든 미생물 감염의 70%가 바이오필름과 연관되어 있다고 추정하고 있으며, 기존의 항생제에 내성이 있는 변종의 출현은 전세계 공중 보건에 심각한 위협이 되고 있다. 따라서 대체의학 물질을 찾아 내는 것은 통합(동서양) 의학의 중요한 화두이다. 이 연구에서 Klebsiella pneumoniae의 바이오 필름 형성과 활성화에 대한 네 가지 허브 추출물을 스크린 하였다. 본 실험에 사용된 식물 추출액 중에 Scutellaria scordifolia Fisch. ex Schrank 추출물이 박테리아의 성장과 바이오 필름 형성에 억제 효과를 보여 주었다. Scutellaria scordifolia Fisch. ex Schrank 추출물이 L929세포에 대한 세포독성을 띄지 않음을 확인 하였다. Scutellaria을 첨가한 실험군에서 다른 대조군에 비해 K. pneumoniae의 성장이 억제되었다. Scutellaria scordifolia Fisch. ex Schrank 추출액이 첨가되지 않은 대조군과 비교하면, 식물 추출액이 첨가된 실험군에서는 생균수가 최대 약 60배 억제되었음을 확인 하였다. Scutellaria scordifolia Fisch. ex Schrank 추출물이 포함된 실험 군에서 K. pneumoniae 균의 개체수가 줄어들어 있음을 differential interference contrast (DIC)의 이미지를 통해서도 확인하였으며, 형광세기의 비교분석을 통해 음성대조군에 비해 추출물이 포함된 실험군에서 형광세기가 통계학적으로 유의하게 낮아져 있는 것을 확인하였다. 이러한 결과는 이 식물 추출물이 K. pneumoniae의, 특히 바이오필름 형성에서 항균제로서 적용될 수 있음을 암시해 주는 것이다.

• 동아시아식생활학회지
• /
• 제16권2호
• /
• pp.199-206
• /
• 2006

This study was designed to investigate the effect of medicinal herb extract on the antioxidant and antimicrobial activities against Helicobacter pylori, which is known as a ulcerogenic pathogen. The concentration of total phenolic compound of Scutellaria baicalensis(13.14%) was highest among water extracts and that of Ulmus parvifolia(15.12%) was highest among the ethanol extracts. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 91.00% and 65.03%, respectively, in DPPH assay. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 32.90% and 27.70%, respectively, in SOD assay. The antioxidant activity of the water extract of Ulmus parvifolia and of the ethanol extract of Glycyrrhiza uralensis Fischer was 2.15 and 2.17, respectively, in TBARS assay. In disc method, Scutellaria baicalensis showed the highest anti-microbial activity against H. pylori, followed by Glycyrrhiza uralensis Fischer among the water extracts and Glycyrrhiza uralensis Fischer showed the highest anti-microbial activity followed by Radix puerariae among ethanol extract.

• KSBB Journal
• /
• 제13권3호
• /
• pp.259-267
• /
• 1998

Optimal feeding strategies in bioreactor operation of Scutellaria baicalensis G. plant cell culture were investigated to maximize the production of flavone glycosides by using a structured kinetic model which can predict culture growth and flavone glycosides synthesis in a rigorous, quantitative manner. For the production of baicalin and wogonin-7-0-GA, the strategies for glucose feeding into Scutellaria baicalensis G. plant cell culture were proposed based on the model, which are a periodic fed-batch operation with maintenance of cell viability and of specific production rate respectively, and a perfusion operation with maintenance of specific production rate for baicalin and wogonin-7-0-GA. Simulation results showed that the highest volumetric concentration of flavone glycosides was obtained in a periodic fed-batch operation with maintenance of cell viability among all the suggested strategies. In the periodic fed-batch operations, the higher volumetric production of flavone glycosides was achieved compared with that in the perfusion operation. It can be concluded that a periodic fed-batch operation with maintenance of cell viability would be the optimal and practical operating strategy of Scutellaris baicalensis G. plant cell culture for the production of flavone glycosides.

• 한국자원식물학회지
• /
• 제14권2호
• /
• pp.148-151
• /
• 2001

한약재로 수요가 많고 꽃이 아름다워 화훼용으로 개발 가능성이 있는 황금을 활성탄을 사용하여 분화재배를 실시할 경우 꽃의 수명이 오래가고 수확시 품질이 양호한 한약재를 생산 할 수 있을 것으로 기대되어 본 시험을 수행 한 결과는 다음과 같다. 1. 황금의 생장은 활성 탄(Activated Charcoal) 30%가 첨가되었을때 경장과 경태가 크고 가지수가 많아 생육이 가장 양호하였다. 2. 황금의 개화는 각 처리 공히 90%이상이 개화되었다. 3. 수량은 활성탄(Activated Charcoal)이 30%첨가되었을 때 생육이 양호하여 근경과 근장이 크고 근중이 무거워서 증수되는 경향이었다. 이와 같은 이유는 활성탄 시용에 의한 토양의 보수력과 보비력이 양호하여 물리성이 향상된 것으로 생각되며 이보다 더 낮은 농도나 높은 농도에서는 효과가 적은것으로 나타났다. 이상과 같은 결과로 보아 황금을 재배할 경우 활성탄(Activated Charcoal)의 효과가 인정되어 약초의 종류에 따른 농도별 시험이 계속적으로 이 루어 져야 할 것으로 사료된다.

• 생명과학회지
• /
• 제10권3호
• /
• pp.236-240
• /
• 2000

한번의 생약제로 쓰고 있는 황금(Scutellaria baicalensis Georgi)에서 얻은 추출물에 대하여 유지에 대한 항산화 효과를 검토하였다. 즉 황금 추출물을 linoleic acid에 소정량 첨가하고 5$0^{\circ}C$에 일정기간 저 장하여 linoleic acid의 산화 안정성을 비교 검토하였으며, 또한 유지 자동산화의 촉진인자인 금속이온 (Fe3+)을 첨가하여 황금 추출물의 금속이온 봉쇄효과를 조사하였다. 황금 추출물의 항산화 효과는 전 농도별에서 높은 항산화 효과를 나타내었다. 그리고 시간 경과에 따른 항산화 효과에 있어서도 정도의 차이는 있지만 전 농도에서 유도기의 연장효과가 상당히 우수한 것으로 나타났다. 황금 추출물의 금속 이온 봉쇄효과에 있어서는 linoleic acid에 황금 추출물과 Fe3+을 첨가한 구가 황금 추출물의 무첨가구보 다 항산화 효과가 아주 우수한 것으로 나타나 금속이온 봉쇄력이 강한 것으로 나타났다.

• 동의생리병리학회지
• /
• 제31권1호
• /
• pp.59-64
• /
• 2017

Scutellaria baicalensis Georgi is one of major herbs used to treat patients with skin diseases diagnosed by wind-heat syndrome. This study was designed to investigate the effects of Scutellaria baicalensis Extract (SBE) on skin lesion of Contact dermatitis (CD) in mice. We investigated the effects of SBE on skin thickness, skin lesions, erythema index and melanin index in vivo. The effects on body weights and spleen/body weight ratio were also investigated in mice with CD induced by topical application of 1-Fluoro-2,4-dinitrofluorobenzene (DNFB). In our results, topical application of SBE lowered skin thickness significantly. In addition, SBE ameliorated skin lesions such as erythema, exudate and petechia in mice with CD. SBE also inhibited erythema index elevated by DNFB significantly. finally, SBE did not affect body weight and spleen/body weight ratio. In conclusion, these data imply that SBE can ameliorate skin lesion of CD such as erythema, exudate and petechia, and be used to treat CD patients with relative safety.

• 패션비즈니스
• /
• 제14권4호
• /
• pp.174-184
• /
• 2010

This research was carried out to find the dyeing properties of Scutellaria baicalensis Georgi on the rayon and cotton fabrics. The best proper dyeing conditions were examined by changing dye concentrations, dyeing temperature, dyeing time, and dyeing repitition. Also dyeability and surface color changes were evaluated by various mordanting methods and agents. The maximum absorption of Scutellaria baicalensis Georgi were at 275nm and 362nm. The optimum dyeing conditions on the cotton were 80%, $80^{\circ}C$, 40minutes, 4 repitition, and those on the rayon were 80%, $80^{\circ}C$, 60minutes, 5 repitition. The K/S values of rayon and cotton fabrics were higher in pre-mordant than post-mordant. The K/S values on the rayons were lower than no mordant fabric except pre-Fe mordant, these on the cotton fabrics were higher than no mordant fabric except post-Al mordant. The value of ${\Delta}E$ was the highest in Fe mordanted fabrics, and higher on the cotton fabric than rayon. The surface colors of all mordanted fabrics were yellow.

• 동의생리병리학회지
• /
• 제19권4호
• /
• pp.1027-1031
• /
• 2005

In the present study, wogonin, a major flavone isolated from Scutellaria Radix were examined for its imunosuppressive activity on the 2,4-dinitro-fluorobenzene (DNFB)-induced delayed type hypersensitivity (DTH) in C3H/HeN mice. This compound inhibited selectively $TNF-\alpha$ and IL-4, but not IL-6, IL-10 and $IFN-\gamma$ on DNFB-induced Th1 and Th2 cytokines in a concentration-dependent manner. Interestingly, this compound increased the expression of heme oxygenase-1 (HO-1) in a concentration-dependent manner The above results reveal that wogonin possesses anti-inflammatory, humoral and cellular immunomodulatory, and stress reducing activities on the DNFB-induced DTH in mice and these properties may contribute to the anti-atopic dermatitis activity of Scutellaria Radix.