• Title/Summary/Keyword: Screening system

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Analysis on the screening effects of elevated railway by measuring the electromagnetic induction in high-speed railway system (고속 전철 시설에 의한 전자 유도의 고가 차폐 실측 연구)

  • Choi, Mun-Hwan;Lee, Sang-Mu;Han, Man Dae;Cho, Pyoung-Dong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.10a
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    • pp.850-853
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    • 2009
  • 통신선에 장애를 유발할 수 있는 전자유도 대책을 위해서 국가 기술기준에 의한 유도전압 한계치가 정해져 있고, 현장 유도 대책 상 이 제한치를 초과할 때에 필요한 것이므로 정부 고시의 기술 근거에 의해 유도전압을 계산하여 이에 대한 조치를 시행하고 있다. 전자유도 현상에 있어서 피유도원인 통신선간의 커플링 관계에 있어서 중간 차폐를 줄 수 있는 성분에 대한 계수치를 적용할 수 있다. 고시에 의하면 고속전철 시설 중 국내의 건설기반이 되는 고가의 교량구간에 의한 차폐 계수를 사용할 수 있고 그 수치가 제시되어 있는 바 이 수치에 대한 실증적 연구 소요에 의하여 교량구간이 아닌 노반 구간과 교량 구간의 경계점에서 비교 측정을 수행하였고 교량에 의한 차폐효과가 현재 고시에 주어진 수치에 근접함을 확인하였다.

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Interaction of Nitric Oxide and Renin Angiotensin System in Pulmonary Arterial Circulation of RHR

  • Lee, Byung-Ho;Shin, Hwa-Sup
    • Archives of Pharmacal Research
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    • v.20 no.5
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    • pp.389-395
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    • 1997
  • We investigated the interaction between nitric oxide and the renin angiotensin system in regulating isolated pulmonary arterial tension and pulmonary arterial pressure (PAP) in renal hypertensive rats (RHR) made by complete ligation of left renal artery. Losartan induced a depressor response that was smaller in RHR than in normotensive rats (NR) (3.3 and 7.0 mmHg, respectively, at 3.0 mg/kg, p<0.05), and the response was significantly reduced by $N^{G}$-nitro-Larginine methyl ester (L-NAME). Angiotensin II elevated the PAP (7.6 and 10.8 mmHg at $0.1 {\mu}g/kg$; 20.3 and 23.6 mmHg at $1.0{\mu}g/kg$, respectively) and contracted the isolated pulmonary artery ($pD_2$: 8.79 and 8.71, respectively) from both NR and RHR with similar magnitude, and these effects were significantly enhanced by L-NAME in NR, but not in RHR. Acetylcholine lowered the PAP slightly less effectively in RHR than in NR (3.8 and 6.0 mmHg at 10 .mu.g/kg, respectively) and relaxed the pulmonary artery precontracted with norepinephrine in both rats with similar magnitude ($E_max$: 60.8 and 63.6%, respectively), and the effect being completely abolished after pretreatment.with L-NAME or removal of endothelial cells. These results suggest that nitric oxide interacts with renin angiotensin system to control the pulmonary vascular tension and pulmonary arterial circulation of RHR.R.

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Screening of Promoters from Metagenomic DNA and Their Use for the Construction of Expression Vectors

  • Han, Sang-Soo;Lee, Jin-Young;Kim, Won-Ho;Shin, Hyun-Jae;Kim, Geun-Joong
    • Journal of Microbiology and Biotechnology
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    • v.18 no.10
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    • pp.1634-1640
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    • 2008
  • This study was focused on the screening of valuable genetic resources, such as promoters from metagenome, and describes a promoter trapping system with a bidirectional probe concept, which can select promoters or operons from various biological resources including metagenomic DNA. A pair of reporters, GFP and DsRed, facing the opposite direction without promoters, is an effective system that can function regardless of the direction of inserted promoters. The feasibility of this system was tested for the isolation of constitutively expressed promoters in E. coli from a soil metagenome, resulting in a potential pool of various promoters for practical application. The analyses of structural organization of the trapped genes demonstrated that constitutively expressible promoters in E. coli were broadly distributed within the metagenome, and suggested that some promoters were useful for the construction of expression vectors. Based on these observations, three constitutive promoters were employed in the expression vector system and their potentials for practical application were evaluated in terms of expression level, protein solubility, and effects on host growth.