• Title/Summary/Keyword: Screening Time

Search Result 1,098, Processing Time 0.043 seconds

Gene Transfer into Pig and Goat Fetal Fibroblasts by Co-transfection of tPA Transgene and NeorNeor Gene

  • Kim, Bae-Chul;Han, Rong-Xun;Kim, Myung-Yoon;Shin, Young-Min;Park, Chang-Sik;Jin, Dong-Il
    • Reproductive and Developmental Biology
    • /
    • v.33 no.2
    • /
    • pp.107-111
    • /
    • 2009
  • The transfection efficiency of a transgene into pig and goat fetal fibroblast cells (PFF and GFF, respectively) was tested using co-transfection of a human tissue-type plasminogen activator (tPA) transgene and neomycin-resistant (NeorNeor) gene, followed by G418 selection. To initially test G418 resistance, GFF and PFF were incubated in culture medium containing different concentration of G418 for 2 weeks, and cell survival was monitored over time. Based on the obtained results, the concentrations chosen for G418 selection were 800 ug/ml and 200 ug/ml for GFF and PFF, respectively. For co-transfection experiments, the pBC1/tPA and NeorNeor vectors were co-transfected into GFF and PFF (1×1061×106 cells in each case) using the FuGENE6 transfection reagent, and resistant colonies were obtained following 14 days of G418 selection. We obtained 96 and 93 drug-resistant colonies of GFF and PFF, respectively, only 54 and 39 of which, respectively, continued proliferating after drug selection. PCR-based screening revealed that 23 out of 54 analyzed GFF colonies and 5 out of 39 analyzed PFF colonies contained insertion of the tPA gene. Thus, the experimentally determined transfection efficiencies for tPA gene co-transfection with the NeorNeor gene were 42.6% for GFF and 12.8% for PFF. These findings suggest that co-transfection of a transgene with the NeorNeor gene can aid in the successful integration of the transgene into fetal fibroblast cells.

Demographic Survey of Four Thousand Patients with 10 Common Cancers in North Eastern Iran over the Past Three Decades

  • Nikfarjam, Zahra;Massoudi, Toktam;Salehi, Maryam;Salehi, Mahta;Khoshroo, Fahimeh
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.23
    • /
    • pp.10193-10198
    • /
    • 2015
  • Background: Cancer is a major cause of mortality in developing countries and correct and valid information about the epidemiology of this disease is the first step in the planning of health care in each region. The aim of this study was to determine the relative frequency, mean age and sex ratio of the most 10 common non-skin cancers in the world and Iran, among patients referred to an oncology clinic. Materials and Methods: This descriptive study was conducted in Mashhad, north east of Iran. The data obtained from the records of patients referred to the private oncology center between the years of 1985-2012". According to the latest report of GLOBOCAN study commonest malignancies included were lung, breast, colorectal, prostate, stomach, liver, cervix, esophageal, bladder cancers and Non-Hodgkin lymphoma. Results: A total of 4,606 cases were analyzed. The mean age was 55.5±13.8years55.5±13.8years (male: 59.5±13.959.5±13.9, female: 52.6±12.952.6±12.9). Overall, breast cancer (1,264 cases, relative frequency of 27.4%) was the most prevalent cancer; however the mean ages of diagnosis were not significantly different between 5-year time period divisions (p=0.290). The most common cancer in men was esophageal cancer (26.3%).The lowest mean age was related to women diagnosed with breast cancer (48.5±11.848.5±11.8) and men with non-Hodgkins lymphoma (48.4±17.848.4±17.8). There were statistically significant differences between the mean age of men and women with gastric (p=0.003) and esophageal cancers (p<0.001). Male to female sex ratios in our study for bladder, lung and stomach cancers were 6.57, 2.60 and 2.50 respectively. Conclusions: The results showed that breast cancer tends to be found in younger female patients and bladder cancer appears more often in men. Screening in target population in addition to early diagnosis may reduce death and disability.

Clinical Outcome of Turkish Metastatic Breast Cancer Patients with Currently Available Treatment Modalities - Single Center Experience

  • Cabuk, Devrim;Basaran, Gul;Teomete, Mehmet;Dane, Faysal;Korkmaz, Taner;Seber, Selcuk;Telli, Ferhat;Yumuk, Perran Fulden;Turhal, Serdar
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.1
    • /
    • pp.117-122
    • /
    • 2014
  • Background: Breast cancer is the most common malignancy and the second leading cause of cancer-related death among women in the developed countries. Despite advances in screening, improved local therapies and adjuvant systemic treatments, median survival of metastatic breast cancer patients (MBC) is in the range of 2-3 years at most. We aimed to investigate whether the prognostic factors and therapeutic responses of our Turkish patients are similar to those in the literature. Materials and Methods: We reviewed the medical records of MBC patients who had been treated in our institution between 1999-2009 and analyzed their clinicopathological features and survival outcomes retrospectively Results: A hundred and sixty patients were included. Median age was 47 (23-82), median follow up was 24 (2-186) months. At the time of diagnosis 59% of patients were under the age of 50 and 46% were postmenopausal. The majority (37%) had multiple sites of metastases. Forty percent received endocrine therapy and 40% chemotherapy as first line metastatic treatment. Thirty (20%) patients were treated with molecular targeting agents like trastuzumab, lapatinib and sunitinib, frequently combined with a chemotherapy agent. Five-year overall survival (OS) was 32% and median OS was 38 months for the whole group. Five year progression free survival (PFS) was 10% and median PFS was 10 months. Menopausal status, hormone receptor expression and disease free status had a significant impact on overall survival in the multivariate analysis (p 0.018, p 0.018 and p:0.003, respectively). Conclusions: All our patients were treated with the modern oncologic therapies recommended by the international guidelines. From our data, MBC patients live up to 3-4 years, indicating that further improvement beyond that requires development of new treatment modalities. The survival outcomes of our patients were consistent with the data reported in the literature.

The Effect of Equatorial Spread F on Relative Orbit Determination of GRACE Using Differenced GPS Observations (DGPS기반 GRACE의 상대궤도결정과 Equatorial Spread F의 영향)

  • Roh, Kyoung-Min;Luehr, Hermann;Park, Sang-Young;Cho, Jung-Ho
    • Journal of Astronomy and Space Sciences
    • /
    • v.26 no.4
    • /
    • pp.499-510
    • /
    • 2009
  • In this paper, relative orbit of Low Earth Orbit satellites is determined using only GPS measurements and the effects of Equatorial Spread-F (ESF), that is one of biggest ionospheric irregularities, are investigated. First, relative orbit determiation process is constructed based on doubly differenced GPS observations. In order to see orbit determination performance, relative orbit of two GRACE satellites is estimated for one month in 2004 when no ESF is observed. The root mean square of the achieved baselines compared with that from K-Band Ranger sensor is about 2~3 mm and average of 95% of ambiguities are resolved. Based on this performance, the relative orbit is estimated for two weeks of two difference years, 2003 when there are lots of ESF occurred, and 2004 when only few ESF occurred. For 2003, the averaged baseline error over two weeks is about 15 mm. That is about 4 times larger than the case of 2004 (3.6 mm). Ionospheric status achieved from K-Band Ranging sensor also shows that more Equatorial Spread-F occurred at 2003 than 2004. Investigation on raw observations and screening process revealed that the ionospheric irregualarities caused by Equatorial Spread-F gave significant effects on GPS signal like signal loss or enhancement ionospheric error, From this study, relative orbit determination using GPS observations should consider the effect of Equatorial Spread-F and adjust orbit determination strategy, especially at the time of solar maximum.

The Efficacy of Fluorograb for Paediatric Patients Dose Reduction during Pneumatic Reduction and Voiding Cystourethrography (VCUG) (영아/유아의 공기 주입 정복술 및 방사선 배뇨성 방광요도 조영술시 피폭 선량 경감을 위한 FluroGrab의 유용성)

  • Kim, Sang-Tae;Choi, Ji Won;Han, Tae-Jong
    • Proceedings of the Korea Contents Association Conference
    • /
    • 2009.05a
    • /
    • pp.1167-1172
    • /
    • 2009
  • The Pneumatic Reduction and VCUG (Voiding Cystourethrography) are commonly used in the paediatric age group. The procedures had a particularly long fluroscopic screening time, despite a successful outcome for paediatric patients. Pneumatic Reduction and VCUG almost invariably requires fluoroscopic guidance which does confer a radiation dose. This article contains suggestions on how the radiation dose to paediatric patients from Pneumatic Reduction and VCUG can be made "as low as reasonably achievable" (ALARA). The aim of our study was eliminated in spot image applying the FluoroGrab, which has function of capturing an image of interest area from the picturing while fluoroscopic procedures. FluoroGrab has clinical value equivalent to the spot image, and is applied to the most recent fluoroscopic procedures. The radiologist and the radiographers should consider new option for decreasing the radiation exposure delivered to paediatric patients by making equipment modifications to the fluoroscopy to optimize radiation exposure reduction techniques. Thus, we propose the FluoroGrab instead of spot exposure for the reduction of patient exposure dose in paediatric, and try to confirm the effect of the mitigating amount of radiation exposure to paediatric patients when pneumatic reduction and VCUG. Fluorograb is the safe and useful method that shows the equivalent level of accuracy to spot exposure, and to minimize the radiation load to paediatric patients are to be the substitute for the spot exposure for Pneumatic Reduction and VCUG.

  • PDF

Frequency of EGFR Mutations in Non-small Cell Lung Cancer Patients: Screening Data from West Siberia

  • Gervas, Polina;Ivanova, Anna;Vasiliev, Nikolay;Ananina, Olga;Zharkova, Olga;Rogovieva, Olga;Verzhbitskaya, Natalia;Didichuk, Ivan;Cheremisina, Olga;Popova, Natalia;Goldberg, Victor;Cherdyntsev, Evgeny;Choynzonov, Evgeny;Cherdyntseva, Nadezda
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.16 no.2
    • /
    • pp.689-692
    • /
    • 2015
  • Background: Incorporation of molecular analysis of the epidermal growth factor receptor (EGFR) gene into routine clinical practice has shown great promise to provide personalized therapy of the non-small cell lung cancer (NSCLC) in the developed world. However, the genetic testing of EGFR mutations has not yet become routine clinical practice in territories remote from the central regions of Russia. Therefore, we aimed to study the frequency of major types of activating mutations of the EGFR gene in NSCLC patients residing in West Siberia. Materials and Methods: We examined EGFR mutations in exons 19 and 21 in 147 NSCLC patients (excluding squamous cell lung carcinomas) by real time polymerase chain reaction. Results: EGFR mutations were detected in 28 of the 147 (19%) patients. There were 19 (13%) cases with mutations in exon 19 and 9 cases (6%) in exon 21. Mutations were more frequently observed in women (42%, p=0.000) than in men (1%). A significantly higher incidence of EGFR mutations was observed in bronchioloalveolar carcinomas (28%, p=0.019) and in adenocarcinomas (21%, p=0.024) than in large cell carcinomas, mixed adenocarcinomas, and NOS (4%). The EGFR mutation rate was much higher in never-smokers than in smokers: 38% vs. 3% (p=0.000). The frequency of EGFR mutations in the Kemerovo and Tomsk regions was 19%. Conclusions: The incorporation of molecular analysis of the EGFR gene into routine clinical practice will allow clinicians to provide personalised therapy, resulting in a significant increase in survival rates and improvement in life quality of advanced NSCLC patients.

A Systematic Review and Meta-analysis of Sensory Integration Intervention Studies in Children with Cerebral Palsy (뇌성마비 아동의 감각통합 중재 연구에 대한 체계적 고찰과 메타분석)

  • Kim, Eun-Joo;Choi, Yoo-Im
    • Journal of Digital Convergence
    • /
    • v.11 no.4
    • /
    • pp.383-389
    • /
    • 2013
  • The purpose of this study was to investigate evidence-based practice (EBP) for sensory integration (SI) intervention in children with cerebral palsy (CP) through a systematic review and meta analysis. The screening strategy was performed to select studies for analysis after that, a meta-analysis was implemented for calculating the effect size (ES) in group studies. Twenty-four studies were included for a systematic review and included seven case reports, three single-subject designs, and fourteen group experimental design studies(three randomized controlled trials, three two groups nonrandomized studies, and six one group nonrandomized studies). The ES of the experimental group studies was moderate size of 0.272. The results of the ES according to the dependent variables, the ES was the largest in the fine motor development. The effect size of the published papers was greater than the unpublished paper's and two groups nonrandomized studies' size effect was the largest in the design. The ES of the infants was larger than the children. The ES for a period of 8 weeks, the number of five times a week, and time in 90 minutes showed the biggest in SI program. Although the ES of SI intervention in children with CP showed moderate effect, accumulation of research well be needed.

Evaluation of Automatic Image Segmentation for 3D Volume Measurement of Liver and Spleen Based on 3D Region-growing Algorithm using Animal Phantom (간과 비장의 체적을 구하기 위한 3차원 영역 확장 기반 자동 영상 분할 알고리즘의 동물팬텀을 이용한 성능검증)

  • Kim, Jin-Sung;Cho, June-Sik;Shin, Kyung-Sook;Kim, Jin-Hwan;Jeon, Ho-Sang;Cho, Gyu-Seong
    • Progress in Medical Physics
    • /
    • v.19 no.3
    • /
    • pp.178-185
    • /
    • 2008
  • Living donor liver transplantation is increasingly performed as an alternative to cadaveric transplantation. Preoperative screening of the donor candidates is very important. The quality, size, and vascular and biliary anatomy of the liver are best assessed with magnetic resonance (MR) imaging or computed tomography (CT). In particular, the volume of the potential graft must be measured to ensure sufficient liver function after surgery. Preoperative liver segmentation has proved useful for measuring the graft volume before living donor liver transplantations in previous studies. In these studies, the liver segments were manually delineated on each image section. The delineated areas were multiplied by the section thickness to obtain volumes and summed to obtain the total volume of the liver segments. This process is tedious and time consuming. To compensate for this problem, automatic segmentation techniques have been proposed with multiplanar CT images. These methods involve the use of sequences of thresholding, morphologic operations (ie, mathematic operations, such as image dilation, erosion, opening, and closing, that are based on shape), and 3D region growing methods. These techniques are complex but require a few computation times. We made a phantom for volume measurement with pig and evaluated actual volume of spleen and liver of phantom. The results represent that our semiautomatic volume measurement algorithm shows a good accuracy and repeatability with actual volume of phantom and possibility for clinical use to assist physician as a measuring tool.

  • PDF

The Major Elements of Psychological Assessment and Intervention for Children and Adolescents after a Disaster: A Professional Delphi Preliminary Survey (재난 시 소아청소년 정신건강 평가 및 치료의 주요 요소: 전문가 델파이 예비 조사)

  • Park, Jang-Ho;Lee, Mi-Sun;Chang, Hyoung Yoon;Hwang, Jun-Won;Lee, Ju-Hyun;Kim, Ji-Youn;Lee, Cheol-Soon;Kim, Eunji;Bae, Seung-Min;Bhang, Soo-Young
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
    • /
    • v.27 no.3
    • /
    • pp.164-172
    • /
    • 2016
  • Objectives: This study attempted to evaluate the usefulness and direction of development of post-traumatic assessment and interventions based on the opinions of psychiatrics and disaster and trauma-related experts using the Delphi survey technique. Methods: In-depth individual interviews served as the pre-survey and were followed by Delphi primary and secondary surveys. Specialists in child and adolescent mental health, psychological support professionals specialized in disasters and related practitioners with experience of disasters in Korea completed a set of questionnaires and participated in focus group interviews and in-depth individual interviews on post-traumatic assessment and intervention. Results: We found that the following issues have a significant impact on the interventions after disasters: the proper time of the initial interview in the event of a disaster, assessment notices, aged assessment services, mandatory enforcement measures, scale screening and treatment intervention elements, symptoms degree classification, intervention standardization, the use of a levelled program, care unit environment, and operation plan. Conclusion: This study proposed effective mental health intervention measures and has implications for the development of evaluation treatment protocols after disasters.

Exploring Rape Reports in Newspapers - A Longitudinal Analysis of Four Korean News Dailies between 1990 and 2007 (한국 신문에 나타난 강간보도의 통시적 분석 - 강간통념과 양가적 성차별주의를 중심으로)

  • Lee, Jung-Gyo;Seo, Young-Nam;Choi, Su-Jin
    • Korean journal of communication and information
    • /
    • v.45
    • /
    • pp.425-462
    • /
    • 2009
  • The present study delves into how rape reports are framed in popular Korean newspapers over time. Specifically, the primary purpose of this study was to examine how rape myths and ambivalent sexism and were presented in three Korean newspapers. In order to assess longitudinal trends of rape reports, the sample for the content analysis was drawn from 1990 to 2007. Four newspapers, Chosun-Ilbo, Dongah-Ilbo, Jungang-Ilbo and Hankyoreh, which have been the most popular in terms of circulation and ad revenues, were selected for this longitudinal content analysis. Using random stratified sampling and screening process, A total of 2160 articles were content analyzed for the main study. The results revealed that rape reports appearing in Korean newspapers were overwhelmingly dominated by episodic frame rather than thematic frame. Particularly, It was found that Korean rape reports generally have attributed the cause of rape crimes to the victims. Implications for results were discussed in terms of rape myth and ambivalent sexism theories.

  • PDF