• Journal of Animal Science and Technology
• /
• v.63 no.4
• /
• pp.934-953
• /
• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.2
• /
• pp.703-706
• /
• 2012

• Proceedings of the Korean Magnestics Society Conference
• /
• 2008.12a
• /
• pp.112.2-112.2
• /
• 2008

• Journal of Microbiology and Biotechnology
• /
• v.31 no.2
• /
• pp.240-249
• /
• 2021

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab (Eriocheir sinensis) survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of E. sinensis have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on E. sinensis collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of E. sinensis when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.

• Current Optics and Photonics
• /
• v.5 no.2
• /
• pp.147-154
• /
• 2021

Microfluidic chip technology is a research focus in biology, chemistry, and medicine, for example. However, microfluidic chips are rarely applied in imaging, especially in ghost imaging. Thus in this work we propose a ghost-imaging system, in which we deploy a novel microfluidic chip modulator (MCM) constructed of double-layer zigzag micro pipelines. While in traditional situations a spatial light modulator (SLM) and supporting computers are required, we can get rid of active modulation devices and computers with this proposed scheme. The corresponding simulation analysis verifies good feasibility of the scheme, which can ensure the quality of data transmission and achieve convenient, fast ghost imaging passively.

• Korean Journal of Community Nutrition
• /
• v.11 no.4
• /
• pp.488-501
• /
• 2006

The purpose of this study was to develop various nutrition education materials, including an animated CD, for day care center children and their teachers and in order to apply the nutrition education programs systematically. We evaluated the effect of the nutrition education programs on nutrition knowledge in the first year, and the effect of the nutrition education programs on nutrition knowledge, food attitudes and eating behaviors in the second year in day care center children. In the first year, nutrition education foundational materials, such as the animated CD-ROM, videotape, textbook, panels, posters, slogans, leaflets, picture disks, hand puppets, stickers for compensation and poster boards were developed. The subjects were 537 children (286 boys,251 girls) aged four to six years. In the second year, nutrition education materials, such as the animated CD-ROM and textbook were developed. The nutrition education group included 368 children (177 boys 191 girls) aged four to six years, and the comparison group consisted of 108 children (54 boys,54 girls) Regarding the nutrition knowledge test results in the first year, the pre-test mean of children was 6.82, however, the post-test mean was 11.35 and showed a significant increase (p < 0.001). Regarding the nutrition knowledge test and the food attitude test results in the second yew, the nutrition education group had significantly higher scores than the comparison group in a post-test (p < 0.001). For the eating behavior test results, the post-test means between the comparison group and the nutrition education group were not significant. The test results show that several months of nutrition education is not enough to improve eating behaviors. As a result, nutrition education has improved nutrition knowledge and food attitudes in day care center children but short-term nutrition education was not sufficient to induce a change in eating behaviors. To improve eating behaviors in day care center children, nutrition education programs must be applied to day care center children in addition to their Parents and Persistent education will have to be continued.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.1
• /
• pp.107-111
• /
• 2020

In this study, we examined the maturation and spawning of yellowback seabream Dentex tumifrons using samples collected by monthly bottom trawling in Korean coastal waters from January to December 2018. We analyzed monthly changes in gonadosomatic index (GSI), maturity stage, histology, fecundity, and total length at group maturity. Spawning occurred in May and October. Fecundity varied with total length as F=1.3754TL^3.2664, with a range of 1,220 to 155,625 eggs per female. At group maturity, 50%, 75%, and 97.5% estimates of female total length were 18.4, 20.5, and 25.3 cm, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.1
• /
• pp.67-73
• /
• 2020

The maturity and spawning of the yellow goosefish Lophius litulon were studied using samples collected monthly from January to December 2018 from the coastal waters of Korea. We analyzed monthly changes in maturity stage, gonadosomatic index (GSI), total length (TL) at 50%, 75%, and 97.5% group maturity, egg diameter and fecundity. The spawning period was from January to June. The size of eggs spawned was between 0.30 to 1.28 mm. Fecundity varied between 328,314 to 1,624,783 eggs. The percentage of sexually mature females estimated from a logistic function was over 50% at 48.67 cm TL, 75% at 56.76 cm TL and 97.5% at 74.89 cm TL.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.1
• /
• pp.43-49
• /
• 2020

The maturation and spawning of red seabream Pagrus major were investigated using 1,014 samples collected monthly from January to December of 2018, in the South Sea of Korea. Based on monthly changes in maturity stage and gonadosomatic index, the spawning period was estimated to be between April and August, with peak spawning occuring from May to June. Fecundity varied between 228,996 and 4,544,948 eggs. The relationship between fecundity (F) and fork length (FL) in this species can be expressed by the equation F=0.4869FL^3.9452 (R²=0.7448). Using a logistic function, the percentage of sexually mature females was estimated to be over 50% for fish with a FL of 35.3 cm.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
• /
• 2000.11a
• /
• pp.413-415
• /
• 2000

마이크로스트립 안테나의 일반적인 특징중의 하나는 좁은 대역폭이다. 이 좁은 대역폭은 안테나의 입력 임피던스에 의해서 제한될 뿐 방사 패턴이나 이득의 변화에 의해서는 아니다. 그래서 안테나에서 결합계수를 조절하여 over-coupling을 만든 후 임피던스의 허수성분을 제거하는 경합회로를 사용하여 안테나의 임피던스 대역폭을 증가 시켰다.