• Journal of Ginseng Research
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• v.32 no.4
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• pp.275-278
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• 2008

Ginseng stem fungus gnat, Phytosciara procera, is reported as a ginseng pest for the first time. It is new to science that a member of the family Sciaridae is a ginseng pest. In our observation, larvae of the gnat can penetrate the stem of ginseng, and then they make a shaft in shoot and root. Number of adults captured by yellow sticky traps were peaked in twice, from late July to early August and from late August to early September. In a ginseng field, 29.7% of ginseng damaged by Phytosciara procera is also infected by bacterial disease caused by Erwinia carotobora. However, there is a possibility on environmental-friendly control, as a result of decreasing effect of damage over 85% when remaining a part of berry on peduncle than tatally remove.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.471-475
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• 2016

The dark-winged fungus gnats are one of the most serious fly pests attacking the mushroom cultivation in Korea. They cause severe damage to the artificial sawdust beds used to cultivate mushroom, and reduce the production of button mushroom, Agaricus bisporus, in greenhouses. In this study, we collected nine species of the mushroom flies in order to identify the dominant species of the dark-winged fungus gnat attacking the A. bisporus plantation using the yellow sticky trap in Buyeo-gun, Boryeong-gun, Yongin-si and Chilgok-gun from April to June 2015. The collected samples were used to determine the DNA sequence of the cytochrome c oxidase subunit I (COI) of the nine different species by DNA barcoding. The sequencing results showed that Lycoriella ingenua was the dominant dark-winged fungus gnat species destroying A. bisporus cultivated on the artificial sawdust beds in Korea.

• Research in Plant Disease
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• v.15 no.3
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• pp.262-265
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• 2009

The fungus gnat, Bradysia difformis was examined for its ability to transmit Fusarium oxysporum in PDA culture. Larvae and adults of B. difformis were able to transmit the fungus as ingested and sticking. We constructed GFP-expressed mutants with Fusarium oxysporum, then feed it to larvae of fungus gnat, B. difformis. So that mycelia were placed in the alimentary canal of larva.

• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.420-423
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• 1999

A Sciarid fly, Lycoriella mali was a serious pest in commercial production of the cultivated mushroom, Pleurotus ostreatus. It was found in light trap and compost samples taken from mushroom houses during all growing seasons. The fly population 'was increased with the development of mushroom growth, the larvae occurred about 30 days after spawn inoculation in compost. The highest population was developed spring cultivation season (March to June) and decreased during summer season (August to September). The larvae feed on mycellium and compost; injury to the growing mushroom mycellium and the subsequent consumption of the primordia; they entered and tunneld sporospore stem and infect pinhead formation which would be resulted in decrease of mushroom yield.

• Korean journal of applied entomology
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• v.57 no.2
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• pp.57-64
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• 2018

The fungus gnat, Bradysia difformis, has been recognized as an important pest of greenhouse crops. There is a need for research on the control of the fungus gnat. However, it is difficult to obtain many generations of the fungus gnat for several kinds of research. Indoor propagation is a very useful method for obtaining enough individuals in cases where the need is for larvae in soil. This study was conducted to determine the optimum growing media and temperature conditions for rearing the fungus gnat in the laboratory. Under experimental temperature conditions, hatching, pupation, and eclosion rates were the highest at $20^{\circ}C$. The developmental period of the fungus gnat was shortened with higher temperatures. The greatest number of eggs was an average of 144 at $20^{\circ}C$. Using different types of larvae growth media, the highest hatching rates were 84.7 and 84.4% in water agar and potato disks, respectively. The larval period was the shortest, at 14.7 days, when grown on potato disks. The highest pupation and eclosion rates were 85.2 and 82.6% on potato disks, respectively. The highest number of eggs was an average of 125.6 on potato disks. Regarding the effects of different growth media on the eclosion rate of B. difformis, the highest eclosion rate was 88.4% on the soil mix, and was 50% on oatmeal, 25% on shredded potato. The results of four different inoculation levels of larvae on eclosion rate of B. difformis showed that the highest eclosion rate was 84.7% for 1,000 larvae. The eclosion rate was shortened with a higher number of larvae inoculated/cage. In the growth medium used, 3,000 eggs were better for the initial level of inoculation, showing a relatively high emergence rate and short developmental period. Mass rearing procedures were explained in detail.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.171-178
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• 1998

This study was conducted to investigate the effect of temperature on the development of amushroom-infesting sciarid fly, Bradysia sp. (Diptera: Sciaridae). Egg period was 12.1, 7.0, 4.4, 3.4, and3.2 days, larval period was 38.3, 26.5, 13.4, 13.2, 12.7 days and pupal period was 10.4, 7.1, 4.4, 3.3, 3.2days, and total development period from egg to adult emergence was 60.8,40.6, 22.2, 19.9, and 19.1 daysat 10, 15, 20,25, 28"C, respectively. Development threshold temperature (DT) and effective accumulativetemperatures (ET) were 3.8"C, 74.8DD in eggs, 1.2"C, 321.8DD in larva, and 3.1$^{\circ}$C, 76.5DD in pupa,respectively. The number of eggs laid per female was 107.9, 129.7, 131.8, 86.9, and 82.7 at respectivetemperatures. Preoviposition period was 6.6, 4.4, 2.2, 1.3, 1.8 days, oviposition period 1.5, 1.5, 1.1, 1.1,1.1 days, postoviposition period 2.0, 1.1, 0.9, 0.6, and 0.3 days at th'e temperature of 10, 15, 20, 25 and 28"C, respectively. The longevity of male and female at the temperature was 13.3, 7.8, 5.9,4.1, 3.4 days and10.4, 7.0, 4.2, 3.0, 3.3days, respectively. The optimum temperature for hatchability was estimated to the20$^{\circ}$C and adult emergence was highest at 20$^{\circ}$C. Pupation rate was 50.7, 68.4, 84.3, 86.5, 45.4% at 10, 15,20, 25, and 28"C, respectively. at 10, 15,20, 25, and 28"C, respectively.tively.

• Korean journal of applied entomology
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• v.43 no.2
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• pp.129-134
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• 2004

A simple and easy method was developed to monitor fungus gnats, Bradysia agrestis on sweet pepper in greenhouses. The larvae of B. agrestis were more attracted to potato discs than other sweetpotato, carrot, garlic, radish, and onion discs. The average numbers of B. agrestis found in each disc were 9.7 (potato), 6.0 (sweetpotato), 4.3 (carrot), 0.7 (garlic), 3.8 (radish), and 1.0 (onion). The number of larvae also increased by the size of disc surface and resulted in 2.1 larvae in 10${\times}$10${\times}$10 mm, 3.4 larvae in 20${\times}$20${\times}$10mm, and 3.9 larvae in 30${\times}$30${\times}$10 mm. The adults were more attracted to a yellow sticky trap (50.0 numbers) than a blue (18.0 numbers) or a white (3.7 numbers) traps. The optimal height for installing sticky trap in the greenhouse was determined as less than 50 cm from the rockwool bed based on the adults caught in the traps; 50.0 in less than 50 cm, 4.3 in 50-100 cm, and 2.0 in more than 100 cm height.

• Korean journal of applied entomology
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• v.40 no.1
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• pp.59-67
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• 2001

The potential of two entomopathogenic nematodes, Sreinernema carpocapsae Pocheon strain and Heterorhabditis bacteriophora Hamyang strain as biological control agents was evaluated against mushroom ny, Lycoriella mali in laboratory and field. Mortality of L. mali was significantly different according to nematode species, concentration, temperature, and developmental stage of fly S. carpocapsae was more effective than H. bacteriophora. Mortality of L. mali was higher at $25^{\circ}C$ than at $20^{\circ}C$. In addition, the 3rd instal and the 4th instar of L. mali were more susceptible than the 2nd instar. The lowest $LC^{50}$ value was represented by S. carpocapsae, 20.0 infective juveniles (Ijs) in the 3rd instar, 27.5 Ijs in the 4th instar at $25^{\circ}C$. S. carpocapsae infected all the developmental stages of L. mali except egg stage and the 1st instar of larva. The highest mortality was shown in adult female representing 74.0% at$20^{\circ}C$ and 80.0% at $25^{\circ}C$.L. mali female adult was influenced by S. carpocapsae in oviposition. The number of eggs by L. mali female infected by nematodes was much lower than uninfected females. S. carpocapsae was dispersed by infected L. mali adult with higher numbers by females than males. When S. carpocapsae was applied at the rate of $2.25{\times}10^{5}\;and\;4.5{\times}10^{5}\;Ijs/1.5\;\textrm{m}^2$ in the mushroom house, mortalities were 42.2% and 81.6%, respectively. The infective juveniles of nematodes survived for 14 days in the mushroom medium. However, nematodes did not affect mushroom growth.

• Journal of Mushroom
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• v.13 no.2
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• pp.145-150
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• 2015

Mushroom sciarid fly Lycoriella ingenua (Dufour), syn. L. mali (Fitch) is one of the most common fly pests affecting the mushroom cultivation in Korea. This study was carried out to investigate the development at different temperatures and damages of L. ingenua in A. bisporus mushroom cultivation. Rearing of mushroom flies were carried out on mycelial culture in Petri dishes. The development of L. ingenua from egg to adult at the temperature of 16, 20, 24, $28^{\circ}C$ were 35.2, 25.8, 23.5, and 22.2 days, respectively. Adult flies invade mushroom farms and oviposit in freshly spawned compost. Damages are mainly caused by the larvae of 1-7 mm, which feed on growing mycelium and also developing stipes and caps of mushrooms. Adult flies spoil the appearance of the fruiting bodies, spread various fungal and bacterial disease, and transmit mites. The damages by mushroom flies increased without pest control causing yield loss of up to 27%.

• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.185-196
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• 2018

The fungus gnat, Bradysia difformis, damages various crops in greenhouses and is recognized as an important pest around the world. Additionally, in the future, many other greenhouse crops will be added to the list of crops damaged by the fungus gnat. In this study, to find effective control methods for the fungus gnat, the insecticidal effect of 20 chemical synthetic insecticides was tested with the potato disc and pot treatment methods; additionally, the control effect of 16 strains of B. thuringiensis was examined with the potato disk method. The fungus gnat larvae were treated for 2 days with each of the synthetic insecticides to determine insecticidal effect using the potato disc method. The results were as follows. Among the highly insecticidal active pesticides, chlorfenapyr exhibited a 100% insecticidal activity, and fenazaquin, acetamiprid, dinotefuran, fenthion and thiamethoxam exhibited more than a 90% insecticidal activity. For the pot treatment method, chlorfenapyr exhibited a 3.3% insecticidal effect, and thiamethoxam, acetamiprid, dinotefuran, fenthion, etc. exhibited an insecticidal effect of less than 10% of the emergence rate to adult fungus gnat after 14 days of treatment. To select the B. thuringiensis strains that have an insecticidal effect on the fungus gnat, 16 strains were biologically assayed using the potato disc method. Among the 16 strains, Bt-3, Bt-8 and Bt-13 had more than a 70% insecticidal effect. The $LC_{50}$ and $LC_{95}$ values of Bt-3, Bt-8 and Bt-13 were $3.7{\times}10^5$ and $4.7{\times}10^8cfu/ml$, $1.4{\times}10^5$ and $1.1{\times}10^7cfu/ml$, and $1.4{\times}10^5$ and $1.3{\times}10^7cfu/ml$, respectively.