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Studies on Root Restoration: Embedding Titanium and Cultured Periodontal Ligament Fibroblasts into the Intradentinal Cavities in Dogs.

  • Yamamoto, T.;Hirata, M.;Iwarnatsu, Y.;Tadatomo, Y.;Shimonishi, M.;Murakami, Y.;Nagaoka, S.;Higuchi, S.;Sato, H.;Kanehira, M.;Kindaichi, K.;Komatsu, M.
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.568.1-568
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    • 2001

  • The purpose of our study is new formation of periodontal ligament (PDL) around titanium implants. In this study, we investigated histologically whether cultured periodontal ligament fibroblasts (CPLFs) would form new PDL on titanium implants in beagle dogs. PDL fibroblasts were obtained from upper premolars of dogs and cultured in ${\alpha}-MEM$ supplemented with 10％ FBS. Some CPLFs were cultured on glass-beads-sandblasted titanium specimen. Artificial intradentinal cavities were prepared through alveolar bone to dentin of lower premolars.(omitted)

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