• Applied Biological Chemistry
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• v.46 no.4
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• pp.291-298
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• 2003

A bacterial strain producing a high level of an extracellular phytase was isolated from livestock waste water, identified as a strain of Pseudomonas fragi and designated as Pseudomonas fragi Y9451. Under the phytase production medium, the activity of phytase reached the highest level after 120 hours of incubation. On the effect of carbon sources on the phytase production, the most favorable carbon source for phytase production was fructose. As for the effect of nitrogen sources, high levels of phytase activity were detected in the medium containing nutrient broth as the nitrogen source. Free $PO_4^{3-}$ inhibited phytase production with increasing concentration of $KE_2PO_4$ and phytate in the media. The addition of $CaCl_2$ and $MgSO_4$ also resulted in the inhibition of phytase production. To investigate the effect of aeration on the phytase production, different volumes of culture broth in Erlenmeyer flasks were incubated in rotary shaker at the speed of 200 rpm. As a result, a high level of phytase activity was detected at small volume of culture broth as compared to larger volume because of its more aerobic condition.

• Archives of design research
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• v.18 no.1 s.59
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• pp.27-38
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• 2005

The purpose of this study is to find the unique image characteristics of Korea and identify the Korea culture by comparing the image characteristics of traditional palaces of Korea, China and Japan. This study identifies the unique image characteristics of Korea through a comparative analysis the image of three nations. Literature research, free association measurement, survey were conducted to select study objects and descriptive words. 5 outside-pictures and 2 inside-pictures of the Changduckung, the Forbidden City, the Nijo castle representing each country's traditional palaces were chosen as study objects. A questionnaire was made with 47 descriptive words and 5-scale measurement. The results of analysis are as follows. The representative words to express the image characteristics of Korea, China and Japan traditional palaces are adornment, stability, openness, lines, unfamiliarity, feminine. This study researched the common and different factors between the image characteristics in traditional palaces of Korea, China and Japan. The common image is the image of lines. The distinctive characteristics of Korea are stability, curved lines and femininity. The characteristics of China are adornment and straight lines. And, the characteristics of Japan are simplicity, unfamiliarity, and openness. Based on the research, it was found out that stability and curved lines are the main characteristics of Korea. This study researches the characteristics of images of Korea, China and Japan. This study shows the common and different factors of image characteristics of in the neighboring three nations of East Asia. Due to the geographical and cultural effect over a long time, the Korea was evaluated as the middle cultural between China and Japan. However, this study found unique characteristics of Korea's image.

• Science of Emotion and Sensibility
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• v.12 no.4
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• pp.501-510
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• 2009

This study is to establish GUI (graphic user interface) in mobile touch phone for grooming user by using two dimensional emotion model determined by multi-dimensional scale method. The processes conducted in the research were as the followings: First of all, visceral, behavioral, and reflective factors of emotion (Norman, 2002) was defined from investigating the life styles of the Grooming users. Secondly, factor analysis was performed to extract the representative emotional words. In the third step, they were mapped into the two-dimensional emotion model through multi-dimensional scaling. Finally, the mapped emotional words were tried to be related to GUI factors of touch phones and normalizing their relation degree between 0 and 1. This study determined GUI factors significantly related to representative emotions described as special, self-centered, sophisticated, free, passionate, neat for application to mobile touch phone. This study determined the major emotion factors that should be considered the most important while designing the GUI factors.

• Journal of Embryo Transfer
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• v.12 no.2
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• pp.133-139
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• 1997

Large scale production of cloned embryos requires the technology of multiple generational nuclear transfer(NT) by using NT embryos itself as the subsequent donor nuclei. In this work we investigated comparatively the effects of enucleated oocytes treated with ionomycin and 6-DMAP on the electrofusion rate and in vitro developmental potential in the first and second NT embryos. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 15 hours after hCG injection. The enucleated oocytes were pre-activated by 5 min incubation in 5$\mu$M ionomycin and 2 hours incubation in 2 mM 6-DMAP at 19~20 hours post-hCG before microinjection. In the first and second generation NT, the unsynchronized 16-cell stage embryos were used as nuclear donor. The separated donor blastomeres were injected into the enucleated activated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of single pulse for 60 $\mu$sec at 1.25kV/cm in $Ca^2$+, $Mg^2$+ - free 0.28 M mannitol solution. In the non-preactivation group, the electrofusion and electrical stimulation was given 3 pulses for 60 $\mu$sec at 1.25 kV/cm in 100$\mu$M $Ca^2$+, $Mg^2$+ 0.28 M mannitol solution. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in TCM-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The results obtained were summarized as follows: 1. In the first generational NT embryos, the electrofusion rate of preactivated and non-activated oocytes(80.4 and 87.8%) was not significantly different, but in the second generational NT embryos, the electrofusion rate was significantly(P<0.05) higher in the non-activated oocytes(85.7%) than in the preactivated oocytes(70.1%). 2) In the first and second generational NT embryos, the developmental potential to biastocyst stage was significantly(P<0.05) higher in the preactivated oocytes(39.3 and35.7%) than in the non-preactivated oocytes(16.0 and 13.3%). No significant difference in the developmental potential was shown between the first and second generational NT embryos derived from the preactivated oocytes. In conclusion, it may be efficient to use the oocytes preactivated with ionomycin and 6-DMAP for the multiple production of cloned embryos by recycling nuclear transfer.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.583-589
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• 2017

The 1900s in New Orleans, the harbor city, was indeed an era of confusion because there were various ethnic groups and races. Songs that had been sung by slaves taken from Africa, Black spiritual music, blues, British folk songs, French folk music, ballet music, Spanish dance music, and the march of military bands were mixed with Rag Time to achieve diversity. This developed the beginning of jazz. While swing jazz was most popular and loved by the public during the 20th century, the bebop preferred the small scale organization of musical instruments and developed as a form of jazz featuring the impromptu musical performances. Later, cool jazz, a new style involving the fast and complicated code progress, emerged with free jazz, which features the fundamental rupture from the tradition of the jazz. Miles Davis, who introduced the rock beat in jazz, started fusion jazz. Although jazz has been named differently depending on the era, the main attraction of jazz lies on improvisation. In other words, despite a small changes in code progress and rhythm, the most important thing the player considers is improvisation. Some famous players who lived in the same era followed the whole atmosphere but each had their own style. So, even when they did play the same song, they revealed their style in solo parts despite the same head.

• Journal of Nutrition and Health
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• v.51 no.2
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• pp.140-152
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• 2018

Purpose: Recently, there has been a worldwide increase in the prevalence of food allergies in children and it may cause nutritional imbalance and poor quality of life for growing children due to dietary restrictions. This study was conducted to quantitatively measure the life burden and related factors of mothers who are the primary caretakers of food-allergic children. Moreover, we generated data for use as a scientific basis for the development of a disease management program to reduce the burden on life from raising food-allergic children. Methods: Mothers of 2,005 children aged 2 years or older and enrolled in Seoul Metropolitan Atopy Free Schools in 2016 who have had diagnosis of food allergy in their lifetime were surveyed. The burden on life of parents with food-allergic children was measured using 17 questions from the Food Allergy Quality of Life-Parental Burden (FAQL-PB) questionnaire, and subjects were asked to respond with a scale of 1 ~ 7. Results: We analyzed the effects of sociodemographic factors of parents and children and the accompanying allergic disease factors on the lives of parents. The number of restricted food items due to food allergy of a child and accompanying diagnosis of atopic dermatitis had a positive (+) effect on the life burden of the parents. Conclusion: Accurate oral food challenge for food allergies should be administered to prevent excessive restriction of food intake. Moreover, efforts should be taken to prevent morbidity accompanying food allergy and relieve symptoms of asthma, allergic rhinitis and atopic dermatitis.

• The KIPS Transactions:PartB
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• v.9B no.2
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• pp.139-146
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• 2002

The robot soccer simulation game is a dynamic multi-agent environment. In this paper we suggest a new reinforcement learning approach to each agent's dynamic positioning in such dynamic environment. Reinforcement learning is the machine learning in which an agent learns from indirect, delayed reward an optimal policy to choose sequences of actions that produce the greatest cumulative reward. Therefore the reinforcement learning is different from supervised learning in the sense that there is no presentation of input-output pairs as training examples. Furthermore, model-free reinforcement learning algorithms like Q-learning do not require defining or learning any models of the surrounding environment. Nevertheless these algorithms can learn the optimal policy if the agent can visit every state-action pair infinitely. However, the biggest problem of monolithic reinforcement learning is that its straightforward applications do not successfully scale up to more complex environments due to the intractable large space of states. In order to address this problem, we suggest Adaptive Mediation-based Modular Q-Learning (AMMQL) as an improvement of the existing Modular Q-Learning (MQL). While simple modular Q-learning combines the results from each learning module in a fixed way, AMMQL combines them in a more flexible way by assigning different weight to each module according to its contribution to rewards. Therefore in addition to resolving the problem of large state space effectively, AMMQL can show higher adaptability to environmental changes than pure MQL. In this paper we use the AMMQL algorithn as a learning method for dynamic positioning of the robot soccer agent, and implement a robot soccer agent system called Cogitoniks.

• Journal of Korea Water Resources Association
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• v.43 no.9
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• pp.801-811
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• 2010

A three-dimensional numerical model called NEWTANK is employed to investigate solitary wave run-up with an internal wave-maker on a steep slope. The numerical model solves the spatially averaged Navier-Stokes equations for two-phase flows. The LES (large-eddy-simulation) approach is adopted to model the turbulence effect by using the Smagorinsky SGS (sub-grid scale) closure model. A two-step projection method is adopted in numerical solutions, aided by the Bi-CGSTAB (Bi-Conjugate Gradient Stabilized) method to solve the pressure Poisson equation for the filtered pressure field. The second-order accurate VOF (volume-of-fluid) method is used to track the distorted and broken free surface. A solitary wave is first internally generated and propagated over a constant water depth in the three-dimensional domain. Numerically predicted results are compared with analytical solutions and numerical errors are analyzed in detail. The model is then applied to study solitary wave run-up on a steep slope and the obtained results are compared with available laboratory measurements.

• Journal of Technology Innovation
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• v.22 no.4
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• pp.145-164
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• 2014

Nuclear fusion is one of the most promising options for generating large amounts of carbon-free energy in the future. Major countries such as China, EU, and Japan have established a national plan for DEMO construction and they are implementing it. Korea has started a nuclear fusion research and development by the KSTAR project started in 1995. There are matured needs for a full-scale research and development initiatives to ensure competition with the major countries for DEMO as well as achieve the final goal to commercialize fusion energy. In this paper, we apply the TRL and AHP methods in order to identify the key technologies to conduct DEMO R&D. We propose the priorities of future R&D on DEMO by deriving a core technology in the field. At first, we review the scientific theory of fusion and trend of progress of DEMO activities in major countries. For previous studies, we review TRL and AHP methods to examine the technology classification system of DEMO and identify key technologies. We apply TRL method to identify readiness level of DEMO technologies and AHP to compensate shortcoming of TRL. The key technologies of DEMO to be secured from a synthesis result of the TRL and AHP are burning plasma, plasma facing material, structural material, high frequency heating, neutral particle beam, safety, plasma diagnostic, and simulation technologies.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.21
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• pp.9301-9305
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• 2014

Background: Pain is one of the most terrifying symptoms for cancer patients. Although most patients with cancer pain need opioids, complete relief of pain is hard to achieve. This study investigated the factors influencing persistent pain-free survival (PPFS) and opioid efficiency. Materials and Methods: A prospective study was conducted on 100 patients with cancer pain, hospitalized at the medical oncology clinic of Akdeniz University. Patient records were collected including patient demographics, the disease, treatment characteristics, and details of opioid usage. Pain intensity was measured using a patient self-reported visual analogue scale (VAS). The area under the curve (AUC) reflecting the pain load was calculated from daily VAS tables. PPFS, the primary measure of opioid efficacy, was described as the duration for which a patient reported a greater than or equal to two-point decline in their VAS for pain. Predictors of opioid efficacy were analysed using a multivariate analysis. Results: In the multivariate analysis, PPFS was associated with the AUC for pain (Exp (B)=0.39 (0.23-0.67), P=0.001), the cumulative opioid dosage used during hospitalisation (Exp (B)=1.00(0.99-1.00), P=0.003) and changes in the opioid dosage (Exp (B)=1.01 (1.00-1.01), P=0.016). The change in VAS score over the standard dosage of opioids was strongly associated with current cancer treatment (chemotherapy vs. others) (${\beta}=-0.31$, T=-2.81, P=0.007) and the VAS for pain at the time of hospitalisation (${\beta}=-0.34$, T=-3.07, P= 0.003). Conclusions: The pain load, opioid dosage, concurrent usage of chemotherapy and initial pain intensity correlate with the benefit received from opioids in cancer patients.