• 제목/요약/키워드: Scab-causing Streptomyces spp.

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제주도 감자 더뎅이병징에서 분리된 Streptomyces spp.의 16S rRNA 유전자 염기서열 분석 (Phylogenetic Differentiation of Streptomyces spp. Isolated from Potato Scab Lesions in Jeju Island of Korea on the Basis of 16S rRNA Gene Sequences)

  • 이수현;고영환;김창진;김범준;이근화
    • 한국미생물·생명공학회지
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    • 제35권4호
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    • pp.347-351
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    • 2007
  • 감자 더뎅이병은 제주도 전 지역에서 발생하는 병해로서 더뎅이병 발생 시 경제적인 손실이 막대한 것으로 알려져 있다. 본 연구에서는 제주도 감자 더뎅이병징이 있는 부위에서 Streptomyces spp.를 분리, 배양한 후, 16S rRNA 유전자를 이용하여 계통분석을 실시하였다. 계통분석 결과 제주도 감자 더뎅이병징이 있는 부위에서 분리된 균은 모두 Streptomyces spp.에 속하였으며, 대부분이 기존에 더뎅이병을 일으키는 Streptomyces spp.로 확인되었다. 그러나 일부의 분리균은 기존에 알려진 감자 더뎅이병원균과는 다르다. 따라서 이들 병원균에 대해서는 지방산과 단백질 분석, 그리고 DNA-DNA 혼성화 등과 같은 보다 많은 연구의 수행을 통하여 새로운 더뎅이병을 일으키는 Streptomyces spp.인지, 또는 아직까지 명명되지 않은 Streptomyces spp.인지를 확인해야 할 것으로 사료된다.

Rapid Identification of Potato Scab Causing Streptomyces spp. from Soil Using Pathogenicity Specific Primers

  • Kim, Jeom-Soon;Lee, Young-Gyu;Ryu, Kyoung-Yul;Kim, Jong-Tae;Cheon, Jeong-Uk
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.134.2-135
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    • 2003
  • The plant-pathogenic species S. scabies, S. acidiscabies, and S. turgidiscabies cause the scab disease of potato and produce the phytotoxins, thaxtomins. necl, a gene conferring a necrogenic phenotype, is involved in pathogenicity and physically linked to the thaxtomin A biosynthetic genes. Identification of the pathogenic strains of Streptomyces from soil was performed through the polymerase chain reaction by using specific pathogenicity primer sets derived from the necl gene sequences of Streptomyces smbies. The DNA was extracted from soil using a bead-beating machine and modifications of the FastPrep system. The DNA was suitable for direct use in the PCR. The PCR products showed the bands of approximately 460 bp. This methods can be very usuful in identifying species responsible for scab diseases and studying on the ecology of plant-pathogenic Streptomyces spp.

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Rapid Identification of Potato Scab Causing Streptomyces spp. Using Pathogenicity Specific Primers

  • Shin, Pyung-Gyun;Kim, Jeom-Soon;Hahm, Young-Il
    • The Plant Pathology Journal
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    • 제18권6호
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    • pp.338-341
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    • 2002
  • The potato scab is caused by several species of Streptomyces. Among these species, only pathogenic strains were found to produce thaxtomin A characterized by necrotic bioassay and HPLC. In this study, identification of the pathogenic strains of Streptomyces was performed through the polymerase chain reaction (PCR) by using specific pathogenicity primer sets derived from the nec1 gene sequences of Streptomyces scabies. The expected PCR products were obtained approximately 580 bp and confirmed by sequencing. This PCR technique can be used effectively to identify the pathogenic Streptomyces species, that cause scab on potato tubers.