• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.331-336
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• 1991

In order to utilize natural cellulosic materials as a fermentative substrate, saccharification of a various kind of native cellulosic materials was performed by using cellulase from the isolated strain, Pseudomonas sp. LBC-505 which potently produced cellulase complex and xylanase. Cellulase complex production was repressed by the low concentration of glucose, induced by cellulosic compounds such as CMC, wheat bran and rice straw et al. and showed to be highest on the PY-CMC medium containing 5% (w/v) wheat bran instead of CMC. Optimal temperature for enzyme reactions of CMCase and xylanase was $50^{\circ}C$, and $55^{\circ}C$ for $\beta$-glucosidase. Optimal pH for these enzyme reaction was 6.6. Rate of saccharification for natural cellulose was low by the treatment of crude enzyme. Among their substrates, rice straw was the most effective substrate of enzymatic reaction in this work. After treating rice straw with 5% (v/v) HC1 and hydrolysing with crude enzyme, rate of saccharification was 18.4% (w/w) on dry substrate. Sugars of cellulosic hydrolyzate mainly contained glucose, xylose and cellobiose.