• 한국수산과학회지
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• 제54권1호
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• pp.9-15
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• 2021

Sargassum serratifolium is a rich source of antioxidant meroterpenoids. The optimal extraction temperature and time for obtaining maximal antioxidant yield, antioxidant activity, and phenolic content from Sargassum serratifolium were determined using response surface methodology (RSM). The ranges of the independent variables for extraction temperature and time were 30-70℃ and 12-36 h, respectively. With increasing temperature and time, the yield increased significantly, while DPPH (2,2-dipheny-1-picrylhydrazyl) radical-scavenging activity and total phenolic content decreased significantly. The optimal extraction temperature and time obtained by RSM were 54℃ and 7 h, respectively, providing a yield of 8.2%, DPPH radical-scavenging activity of 60%, and total phenolic content of 163 mg GAE/g. The findings of this study provide useful information for the development of Sargassum serratifolium extraction processes for the food and cosmetic industries.

• 한국수산과학회지
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• 제50권1호
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• pp.85-91
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• 2017

Alzheimer's disease (AD) is a progressive neurodegenerative disorder of insidious onset that causes gradual loss of memory and cognitive function, and it is the most common form of dementia in the elderly. AD is characterized by neuritic plaques and neurofibrillary tangles in the brain, together with loss of neuronal cells. The major neuropathological hallmark of AD is the accumulation of extracellular neurotoxic ${\beta}-amyloid$ ($A{\beta}$) peptides, such as $A{\beta}1-42$, in the brain. In the present study, we investigated the effect of sargachromenol (SCM), sargaquinoic acid (SQA) and sargahydroquinoic acid (SHQA) isolated from Sargassum serratifoilum ethanol extract (SSE) on $A{\beta}$ production in vitro using APP751-transfected Chinese hamster ovary cells (CHO-751). CHO-751 cells were treated with various concentrations of SSE, SCM, SQA and SHQA, and the level of extracellular $A{\beta}1-42$ was evaluated by enzyme-linked immunosorbent assay. SSE and SHQA reduced the production of $A{\beta}1-42$ in CHO-751 cells. Therefore, SHQA isolated from S. serratifolium has potential as an inhibitor of neurotoxic $A{\beta}$ peptide production.

• 한국수산과학회지
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• 제52권1호
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• pp.81-86
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• 2019

The common hallmark of several neurodegenerative disorders, including Alzheimer's disease (AD), is the presence of chronic neuroinflammation, which contributes to the loss of neuronal structure and function. This study investigated the effects of an ethanolic extract of Sargassum serratifolium (SSE) in a lipopolysaccharides (LPS)-induced murine neuroinflammation model. Mice were administered SSE (100 mg/kg body weight) or vehicle for 5 days by oral gavage, and then treated with LPS or saline by intraperitoneal injection. Thereafter, the brain tissues were collected, and the expression of pro-inflammatory cytokines was analyzed by quantitative real-time RT-PCR. There was a marked increase in the spleen weight index in the LPS-treated groups, which indicated the induction of acute systemic inflammation. Based on significant increases in the levels of IL-1 and IL-6 expression, the induction of neuroinflammation was also evident in the cortex and hippocampus of the LPS-treated groups. The overall expression of IL-1 and IL-6 was decreased slightly by SSE administration, compared with the LPS group, and a marked change in IL-1 was observed in the cortex of the SSE-treated (SSE/LPS) group. These results suggest that SSE has potential as an anti-neuroinflammatory nutraceutical.

• Fisheries and Aquatic Sciences
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• 제22권3호
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• pp.7.1-7.10
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• 2019

Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-${\alpha}$-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-${\alpha}$-induced HUVECs. Moreover, TNF-${\alpha}$-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-${\alpha}$-induced nuclear factor kappa B ($NF-{\kappa}B$) activation via preventing proteolytic degradation of inhibitor ${\kappa}B-{\alpha}$. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-${\alpha}$-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of $NF-{\kappa}B$ activation and the upregulation of Nrf2 activation in TNF-${\alpha}$-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders.

• ALGAE
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• 제21권4호
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• pp.393-405
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• 2006

Eight species of Sargassum subgenus Bactrophycus section Halochloa and Repentia were collected from the coasts of Korea: Sargassum coreanum J. Agardh, Sargassum sagamianum Yendo, Sargassum micracanthum (Kützing) Endlicher, Sargassum macrocarpum C. Agardh, Sargassum autumnale Yoshida, Sargassum siliquastrum (Mertens ex Turner) C. Agardh, Sargassum serratifolium (C. Agardh) C. Agardh, Sargassum yezoense (Yamada) Yoshida et Konno. These species were investigated on morphological variations and geographical distributions in Korea. The valuable taxonomic characters between similar species of these section were discussed with special reference to types of holdfasts, shape of main branch, leaves and vesicles.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2020년도 정기학술대회 발표논문집
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• pp.214-214
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• 2020

• 한국식품과학회지
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• 제48권3호
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• pp.241-246
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• 2016

S. aureus, S. epidermidis, P. aeruginosa, P. acnes와 C. albicans는 사람의 피부에서 발생하는 질병과 밀접한 관련을 가지는 대표적인 병원성 미생물로 알려져 있다. 본 연구에서는 해조류 중에서도 항균 활성에 대한 연구가 미미한 모자반을 대상으로 피부 병원균에 대한 항균 효과를 조사하였다. 국내의 연해에 자생하는 7종의 모자반 추출물 중에서 disc diffusion assay와 MIC assay를 통해 가장 뛰어난 항균 효과를 나타낸 톱니모자반을 후속 연구를 위한 후보 물질로 선정하고 연구를 진행하였다. 톱니모자반 추출물의 유기용매 분획층 중에서, 노말-헥세인 분획층이 S. aureus, S. epidermidis, P. aeruginosa, P. acnes 및 C. albicans에 대한 MIC값이 $32-256{\mu}g/mL$로 가장 뛰어난 항균 활성을 나타내었다. 이에 피부 병원균들에 대한 치료제로 사용되고 있지만 내성균의 출현으로 효능이 거의 없는 항생제들인 테트라사이클린, 에리트로마이신, 린코마이신과 플루코나졸과 항균 활성이 뛰어난 것으로 나타난 톱니모자반의 노말-헥세인 분획층과의 병용 사용에 의한 항균 시너지 효과를 조사하였다. 그 결과, 톱니모자반 노말-헥세인 분획층과 이들 항생제와의 병용 사용에 의해 피부 병원균에 대한 항생제와 톱니모자반 노말-헥세인 분획층의 MIC값이 4-32배 감소되었고, 톱니모자반 노말-헥세인 분획층과 이들 항생제와의 병용 시 median FIC값이 0.26-0.55로 항균 시너지 효과를 나타내었다. 즉, 항생제와 톱니모자반 노말-헥세인 분획층과의 병용 사용은 피부 병원균에 대한 이들 항생제의 감수성을 회복시키는데 크게 기여하는 것으로 나타났다.

• 한국해양바이오학회지
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• 제8권1호
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• pp.30-38
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• 2016

The objective of this study was to investigate the useful components and potential antioxidant activities of 30 marine algae, collected from Jeju Island in Korea. The contents of bioacitve substance of extracts from marine algae, such total polyphenol and carbohydrate, were determined. The extract of Distromium decumbens had the highest amount of total polyphenol content (21.27%), and that of Gracilaria incurvata Okamura had the highest amount of total carbohydrate content (10.18%). The antioxidant activities of extracts obtained from algae were tested through the evaluation of DPPH radical and hydroxyl radical scavenging activity. The extracts of Distromium decumbens, Sargassum hemiphyllum (Turner) C.Agardh, Sargassum serratifolium (C. Agardh) C. Agardh and Acrosorium yendoi Yamada were found to have more than 80% DPPH radical scavenging activity and that of Dictyota okamurae (Dawson) $H{\ddot{o}}tning$, Schnetter, et Prud'homme van Reine, Myagropsis myagroides (Martens ex Turner) Fensholt, Sargassum serratifolium (C. Agardh) C. Agardh and Cladophora wrightiana Harvey showed more than 50% hydroxyl radical scavenging activity. These results suggest that algae collected from Jeju Island would be good raw materials for antioxidant.

• ALGAE
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• 제23권3호
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• pp.231-240
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• 2008

The species composition and variation of benthic marine algae at Daejin and Jukbyeon on the mid-east coast of Korea were investigated seasonally from August 2006 to April 2007. As a result, the total of 85 species, including 8 chlorophytes, 26 phaeophytes, 50 rhodophytes and 1 sea grass were identified. 52 species were found at Daejin and 74 species at Jukbyeon. Dominant species in importance value were Corallina pilulifera, Chondrus ocellatus, melobesioidean algae, Sargassum horneri, Sargassum yezoense and Phyllospadix japonica at Daejin, and Corallina pilulifera, melobesioidean algae, Phyllospadix japonica, Acrosorium polyneurum, Sargassum siliquastrum and Hizikia fusiformis at Jukbyeon, respectively. The vertical distribution of algae were characterized by Corallina pilulifera, Hizikia fusiformis, Sargassum spp. (S. siliquastrum, S. yezoense) and Symphyocladia latiuscula at intertidal zone, Sargassum spp. (S. horneri, S. serratifolium, S. yezoense), melobesioidean algae, Phyllospadix japonica and Corallina pilulifera at 1 m depth, melobesioidean algae, Phyllospadix japonica and Sargassum spp. (S. horneri, S. yezoense) at 5 m depth and melobesioidean algae, Phyllospadix japonica and Sargassum spp. (S. horneri, S. micracanthum, S. yezoense) at 10 m depth.

• Journal of Plant Biology
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• 제31권2호
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• pp.83-89
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• 1988

The Fucales plants have very homogenous taxonomic characteristics at order level, but show many morphological variatations at species or subspecies levels. This study is one of the serial works to obtain more taxonomic data than morphotaxonomic ones and to clarify the taxonomic ranks and characteristics of Korean Fucales plants through physiological and biochemical analyses. Total lipid compositions of Korean Fucales plants were analyzed by the 3-dimensional TLC method. Major components of phospholipids (PA, PC, PG, PE, DPG, LPE, LPC), neutral fat TG and sterols were commonly contained in each species. Unknown lipids X1, X2 and X3 were contained in genus Sargassum, but Hizikia was lacking X3 and Myagropsis was lacking both X2 and X3. The latter contained unique X8. From the view-point of the phylogeny of lipid metabolism, Sargassum might be more closely related to Hizikia than to Myagropsis. A variant of S. patens from Seongsan showed minute metabolic difference from that of the typical plant; but a variant of S. serratifolium did not show any significant difference from that of the typical plant.