• Korean Journal of Veterinary Service
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• v.45 no.4
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• pp.269-275
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• 2022

Blood type in dogs is based on the antigen present on the red blood cell surface. Dog erythrocyte antigen 1 is a crucial red blood cell antigen in dogs, whereas the dog erythrocyte antigen 7 has been studied in limited dog breeds worldwide. To assess the prevalence of dog erythrocyte antigens 1 and 7 in 11 breeds in the Republic of Korea, 624 dog blood samples were examined for antigen detection. Overall, 520 dogs (83.3%) showed dog erythrocyte antigen 1 expression. The distribution varied from 50.0~100.0% according to the breed. Dog erythrocyte antigen 1-positive blood type was the highest in Chihuahua (100%), followed by Jindo dog (98.5%), and Sapsaree (95.3%). Dog erythrocyte antigen 7 was positive in 125 dogs (20.0%), and the positivity varied from 5.0~42.9% according to the breed. Dog erythrocyte antigen 7-positive blood type was the highest in Beagle (42.9%), followed by Chihuahua (37.5%), and Jindo dog (27.8%). The high prevalence of dog erythrocyte antigen 1 is because of the high proportion of Jindo dog and Sapsaree breeds that were mostly positive for the antigen. The high abundance of these breeds could be due to inbreeding and local breeding in the Republic of Korea. To our best knowledge, this study is the first to report on the prevalence of dog erythrocyte antigens 1 and 7 among various canine breeds in the Republic of Korea. The prevalence data obtained from this study may contribute to baseline information on veterinary transfusion medicine in small animal practice.

• Journal of Animal Science and Technology
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• v.52 no.6
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• pp.481-490
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• 2010

This study was conducted to characterize the morphological and behavior patterns of Sapsaree dogs. The population (n=8,256) has been constructed since 1990 over 12 generations and managed at the Sapsaree Breeding Research Institute, Gyeongsan, Gyeongbuk province. Eighteen morphological and seven behavioral traits were investigated for 882 individuals. Linear models were applied for each trait by fitting sex, season of birth, hair color or test age. The averages of body weight, body length, body height, and depth of chest were $20.5{\pm}2.4\;kg$, $57.3{\pm}4.2\;cm$, $52.1{\pm}3.6\;cm$, and $21.1{\pm}2.4\;cm$, respectively. Males had greater estimated values than females for these body conformation traits. The chocolate Sapsarees had greater averages for body weight, body height and chest depth. The older animals (>2 years) had heavier body weight than younger animals. About 54, 69, 97, 39 and 83.3% of the Sapsarees had hairs with yellow color, straight, medium to long, untangled, and longer around eyes, respectively. Also, about 40% brown eye, 43% curly tail, 78% normal jaw, 86% no missing teeth, 90% no dewclaw, and >90% with black nose, pendent ear, tongue without spots were observed. About 95% males were normal in testicles state. The males performed significantly better than the females for majority of the behavioral traits. For nerve stability, affability, wariness, adaptability, sharpness, activity, and reaction during blood drawn about 79%, 73%, 76%, 61%, 70%, 48% and 81% of the Sapsarees performed at desired level. In general, the Sapsarees showed good characteristics for both morphological and behavioral traits, which can be exploited to use the Sapsaree breed as a companion or guide dog.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.8
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• pp.1063-1072
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• 2012

Effective population size ($N_e$) is an important measure to understand population structure and genetic variability in animal species. The objective of this study was to estimate $N_e$ in Sapsaree dogs using the information of rate of inbreeding and genomic data that were obtained from pedigree and the Illumina CanineSNP20 (20K) and CanineHD (170K) beadchips, respectively. Three SNP panels, i.e. Sap134 (20K), Sap60 (170K), and Sap183 (the combined panel from the 20K and 170K), were used to genotype 134, 60, and 183 animal samples, respectively. The $N_e$ estimates based on inbreeding rate ranged from 16 to 51 about five to 13 generations ago. With the use of SNP genotypes, two methods were applied for $N_e$ estimation, i.e. pair-wise $r^2$ values using a simple expectation of distance and $r^2$ values under a non-linear regression with respective distances assuming a finite population size. The average pair-wise $N_e$ estimates across generations using the pairs of SNPs that were located within 5 Mb in the Sap134, Sap60, and Sap183 panels, were 1,486, 1,025 and 1,293, respectively. Under the non-linear regression method, the average $N_e$ estimates were 1,601, 528, and 1,129 for the respective panels. Also, the point estimates of past $N_e$ at 5, 20, and 50 generations ago ranged between 64 to 75, 245 to 286, and 573 to 646, respectively, indicating a significant $N_e$ reduction in the last several generations. These results suggest a strong necessity for minimizing inbreeding through the application of genomic selection or other breeding strategies to increase $N_e$, so as to maintain genetic variation and to avoid future bottlenecks in the Sapsaree population.

• Journal of Animal Science and Technology
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• v.58 no.4
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• pp.17.1-17.7
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• 2016

Background: The Sapsaree (Canis familiaris) is a Korean native dog that is very friendly, protective, and loyal to its owner, and is registered as a natural monument in Korea (number: 368). To investigate large-scale gene expression profiles and identify the genes related to exercise-induced stress in the Sapsaree, we performed whole-transcriptome RNA sequencing and analyzed gene expression patterns before and after exercise performance. Results: We identified 525 differentially expressed genes in ten dogs before and after exercise. Gene Ontology classification and KEGG pathway analysis revealed that the genes were mainly involved in metabolic processes, such as programmed cell death, protein metabolic process, phosphatidylinositol signaling system, and cation binding in cytoplasm. The ten Sapsarees could be divided into two groups based on the gene expression patterns before and after exercise. The two groups were significantly different in terms of their basic body type ($p{\leq}0.05$). Seven representative genes with significantly different expression patterns before and after exercise between the two groups were chosen and characterized. Conclusions: Body type had a significant effect on the patterns of differential gene expression induced by exercise. Whole-transcriptome sequencing is a useful method for investigating the biological characteristics of the Sapsaree and the large-scale genomic differences of canines in general.

• Journal of Veterinary Clinics
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• v.25 no.6
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• pp.482-487
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• 2008

This study was performed to investigate the ophthalmic findings of the Korean traditional 'Sapsaree' dogs, and to provide ophthalmic references. Five hundred forty-seven Sapsaree dogs were randomly chosen without any criteria preference. Ophthalmic examination was performed bilaterally using ocular equipments, including slit-lamp biomicroscope, tonometer, direct and indirect ophthalmoscope. The obtained data for ophthalmic findings were analysis by the multivariate linear regression model to identify effects of sex, age and hair coat type. For congenital conditions, the incidence of persistent pupillary membrane (PPM) was the most common defect and there was present 8.8 % whereas for acquired ophthalmic diseases, cataract was the most commonly observed (3.7 %) in Sapsaree dog. Other frequent ocular finding included persistent hyaloid vessel remnant (PHVR, 6.2 %) in congenital conditions, and postinflammatory retinal degeneration (PIRD, 3.1 %) and corneal dystrophy (2.4 %) in acquired ophthalmic diseases. Only one significant effect was age of the Sapsaree for PIRD (P=0.040). There are no any significant effect for sex and hair coat types. This study will provide useful guidelines to clinicians and breeders for the basic ophthalmic information on this breed.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.936-942
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• 2015

The purpose of this study was to characterize genetic architecture of behavior patterns in Sapsaree dogs. The breed population (n=8,256) has been constructed since 1990 over 12 generations and managed at the Sapsaree Breeding Research Institute, Gyeongsan, Korea. Seven behavioral traits were investigated for 882 individuals. The traits were classified as a quantitative or a categorical group, and heritabilities ($h^2$) and variance components were estimated under the Animal model using ASREML 2.0 software program. In general, the $h^2$ estimates of the traits ranged between 0.00 and 0.16. Strong genetic ($r_G$) and phenotypic ($r_P$) correlations were observed between nerve stability, affability and adaptability, i.e. 0.9 to 0.94 and 0.46 to 0.68, respectively. To detect significant single nucleotide polymorphism (SNP) for the behavioral traits, a total of 134 and 60 samples were genotyped using the Illumina 22K CanineSNP20 and 170K CanineHD bead chips, respectively. Two datasets comprising 60 (Sap60) and 183 (Sap183) samples were analyzed, respectively, of which the latter was based on the SNPs that were embedded on both the 22K and 170K chips. To perform genome-wide association analysis, each SNP was considered with the residuals of each phenotype that were adjusted for sex and year of birth as fixed effects. A least squares based single marker regression analysis was followed by a stepwise regression procedure for the significant SNPs (p<0.01), to determine a best set of SNPs for each trait. A total of 41 SNPs were detected with the Sap183 samples for the behavior traits. The significant SNPs need to be verified using other samples, so as to be utilized to improve behavior traits via marker-assisted selection in the Sapsaree population.

• Journal of Veterinary Clinics
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• v.30 no.2
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• pp.95-99
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• 2013

This study was performed to evaluate the seasonal variation of intraocular pressure (IOP) values in normal Sapsaree dogs. Sapsaree dogs (n = 474) clinically free from ophthalmic disease were included in this study, and both eyes were examined in each dog. An applanation tonometer (Tonopen$^{(R)}$ XL) was used to measure IOP values in both eyes. Data obtained from both eyes were stratified by factors thought to affect IOP values including age, gender, hair coat type as well as season of measurement. The IOP results were compared by ANCOVA to verify the effect each factor had on IOP, and an ANOVA test followed by a Bonferroni post hoc test was used for further analysis. The overall mean ${\pm}$ SD IOP value was $19.1{\pm}3.7$ mmHg (range: 8 to 28 mmHg) in the present study. Only seasonal variation showed a significant effect (P < 0.001), on IOP values. IOP values measured in winter ($20.4{\pm}3.4$ mmHg) and summer ($17.1{\pm}3.6$ mmHg) were significantly higher and lower, respectively, than those measured in other seasons. These results suggest a seasonal variation has a significant effect on the IOP of normal dogs. This may prompt further research and possible modification of current veterinary ophthalmic references.

• Korean Journal of Veterinary Service
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• v.33 no.2
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• pp.207-211
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• 2010

None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.

• Korean Journal of Veterinary Service
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• v.34 no.3
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• pp.291-296
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• 2011

The karyotype of the domestic dog is widely accepted as one of the difficult mammalian karyotypes to work. In contrast to many other animals, knowledge about the canine karyotype is quite sparse. The dog has a total of 78 chromosomes; all 76 autosomes are acrocentric in morphology and show only a gradual decrease in length. But appear to be quite small and difficult to identify unambiguously. To purchased standardization of chromosome in Korea native dog, there were analyzed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with 4, 6, 8, 11, 13, 17 chromosome. There were no variations in karyotypes which were analyzed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with Sapsaree, Jindo, Gyeongju DongGyeong dogs, Welshi-Corgi. It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation. Comparative analysis of the distribution patterns of conserved segments defined by dog paints in the genomes of the Korea native dogs demonstrates that their differences in the karyotypes of these three species could have resulted from acrocentric banding patterns.

• Korean Journal of Veterinary Service
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• v.42 no.3
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• pp.145-152
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• 2019

Canine vector-borne diseases (CVBDs) are transmitted by different groups of hematophagous arthropod vectors that are distributed worldwide and can cause significant health problems for dogs. The aim of this study was to investigate and compare the prevalence of selected CVBD pathogens in rural outdoor dogs based on prevention status. Between June 2017 and February 2019, blood samples were collected from 343 clinically healthy rural dogs composing two different groups: systematically managed dogs (SMD; n=92) and personally managed dogs (PMD; n=251). Vaccination and preventive medications were applied strictly following the programmed schedule for the SMD group; in contrast, in the PMD group, they were applied only when requested by the dog owners. Serological and molecular assessments showed that significantly more dogs in the PMD group were infected with B. gibsoni (P<0.001) and D. immitis (P=0.001) than those in the SMD group. These findings suggest that the regular use of preventive medications and environmental controlling efforts contribute to reducing the prevalence of CVBD pathogen infections. In addition, dogs infected with certain kinds of CVBD pathogens could remain asymptomatic, suggesting that continuous monitoring and periodic preventive treatment should be conducted even for clinically healthy dogs.