• Title/Summary/Keyword: Saponin Content

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Comparative Studies on the Effects of Total, Protopanaxadiol and Protopanaxatriol saponins of Ginseng 1. Their Effects on Lipid and Glucose Content in Rat Serum (인삼 총사포닌, 디올계 및 트리올계 사포닌의 효과 1. 흰쥐 혈청 지질 및 당함량에 미치는 영향)

  • 임창진;박은희;홍순근;이동권
    • Journal of Ginseng Research
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    • v.5 no.1
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    • pp.41-48
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    • 1981
  • Total saponin, protopanaxadiol-saponin and protopanaxatriol-saponin were isolated and purified from the side roots of red ginseng. After we administered them orally into rats during 5 weeks, we observed their effects on lipid and glucose content in rat serum. The change in body weight of protopanaxatriol- saponin treated group was slightly larger than those of other groups. Total lipid content in total saponin treated group showed an increase of about 20 % over that in control group. However, protopanaxadiol-saponin and protopanaxatriol- saponin treated groups showed no change. While triglyceride content in total saponin treated group decreased 29oyo compared to it s content in control group, its content in protopanaxatriol-saponin treated group increased 45%. Three saponin treated groups showed lower value than control group in total ant free cholesterol levels. While glucose content in total saponin treated group decreased slightly, that in Protopanaxadiol-saponin treated group decreased slightly compared to that in control group. And protopanaxatriol- saponin trented group showed the significant decrease of 25%. From these results, it is supposed that total saponin accelerates the conversion of lipid into glucose and that protopanaxatriol- saponin accelerates the conversion of glucose into lipid.

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Variation of Saponin Content in Korean Native Soybean Landraces Reintroduced from USA to Korea (미국으로부터 재도입된 한국 원산 콩 재래종의 Saponin 함량 변이)

  • Jang, Eun-Kyu;Piao, Xiangmin;Hwang, Tae-Young;Kim, Sun-Lim;Chung, Gyu-Hwa;Tsukamoto, Chigen;Choi, Yu-Mi;Lee, Myung-Chul;Lee, Jeong-Ran;Kim, Hong-Sig
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.286-295
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    • 2012
  • This study was carried out to assess the variation of saponin contents in 293 Korean native soybean landraces reintroduced from USA to Korea in 2007. Group A saponin content ranged from 149.8 ${\mu}g\;g^{-1}$ to 1279.0 ${\mu}g\;g^{-1}$ with an average of 484.9 ${\mu}g\;g^{-1}$. Group B saponin content ranged from 2160.1 ${\mu}g\;g^{-1}$ to 7868.6 ${\mu}g\;g^{-1}$ with an average of 3670.0 ${\mu}g\;g^{-1}$. Total saponin content including both group A and B contents ranged from 2502.8 to 8764.0 ${\mu}g\;g^{-1}$ with an average of 4154.9 ${\mu}g\;g^{-1}$. Five promising landraces for use as breeding materials that showed higher than any other landraces in group B and total saponin content were IT226841, IT226761, IT226841, IT226828 and IT228534. IT226841 showed the highest saponin content with group B content of 7868.5 ${\mu}g\;g^{-1}$ and total saponin content of 8764.0 ${\mu}g\;g^{-1}$ among landraces. In the relationship among each components, group A saponin content showed a positive correlation with group B saponin content (r=0.3708) and total saponin content (r=0.5119). Group B saponin content showed a very high positive correlation (r=0.9876) with total saponin content. When landraces were compared for the total saponin content based on collected location, landraces from North Korea showed the highest, Gyeonggi-do showed second highest content, followed by Chungcheongbuk-do, Gangwon-do, Gyongsangbuk-do, Chungcheongnam-do, Gyongsangnam-do, Jeollabuk-do and Jeollanam-do. Landraces were also grouped according to seed size and seed coat color. Small seed group was higher than medium and large seed groups that showed no significant difference in total saponin content. Seed coat colors showed no significant difference in total saponin content.

Saponin and Ginsenoside Content in Korean Red Ginseng Products (고려홍삼 제품류의 사포닌 및 진세노사이드 함량)

  • 최강주;고성룡
    • Journal of Ginseng Research
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    • v.13 no.2
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    • pp.178-182
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    • 1989
  • Red ginseng products manufactured by the Korea Ginseng and Tobacco Corporation were analyzed to determine the crude saponin , total saponin and ginsenoside contentents by gravimetry, spertrometry and HPLC, respectively, to see if effective quality control of the components in the products can be achieved. Medicinal powders, powders, tablets and capsules which were made from ginseng powder showed similarity in saponin content, the ratio of PD to PT saponin, and the ginsenoside content and composition, while extract powder, extract, extract tea, extract pills and tea, which were made of ginseng extract, showed difference in saponin content, ratio of PD to PT saponin, and the content and composition of ginsengside. It is, accordingly, believed that ginseng products which are uniform in contents and saponin composition can be produced by carrying out strict quality control throughout the processes of making raw red ginseng into final products.

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Changes of Saponin Contents of Leaves, Stems and Flower-buds of Panax ginseng C. A. Meyer by Harvesting Days (인삼 지상부의 채취시기에 따른 사포닌조성 비교)

  • Choi, Jae-Eul;Li, Xiangguo;Han, Young-Hwan;Lee, Ki-Teak
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.4
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    • pp.251-256
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    • 2009
  • The parts of leaves, flowers and stems in ginseng were obtained for analyzing the component of saponin on 15th April, 25th April, 5th May, 25th May, which were considered as ginseng foliation stage. The total saponin content of the leaves were 97.29, 66.42, 67.61, 36.24 mg/g, respectively, in which the content of Re, $Rb_1$ and Rd were more than 2/3 amount of total saponin. Especially, the saponin content of leaves decreased according to the sequential collection days, in which the similar results were observed from the flowers and stems of ginseng. The total saponin content of the flowers and stems were 141.09,143.84,139.25,133.47 and 13.32, 9.85, 8.00, 4.65 mg/g, respectively. Among them, the content of Re, Rd and $Rb_2$ in flowers were more than 2/3 while the content of Re, $Rg_1$ and Rd in stems showed more than 9/10 amount of total saponin. The total saponin content of individual leaf were 19.46, 28.56, 58.82 and 169.24 mg/plant, 2.53, 2.76, 5.20 and 12.32 mg/plant in stems, and 14.11, 30.21, 37.60 and 73.41 mg/plant in flowers. Therefore, the total saponin content of aboveground parts in ginseng were leaves > flowers > stems.

Content and Composition of Saponin Compounds of Panax Species (Panax(인삼)속 식물의 사포닌화합물 함량 및 조성)

  • 고성룡;최강주
    • Journal of Ginseng Research
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    • v.19 no.3
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    • pp.254-259
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    • 1995
  • The content and composition of saponin compounds of Panax species were analyzed according to their species, region and processing type of red and white ginseng. The species employed were Korean-, Chinese-, Japanese red ginsengs, and Korean white ginseng of Panax ginseng, American- and Canadian ginsengs of Panax quinquefolium, and Panax notoinseng. Twelve main saponin components in the ginseng were identified and quantified using TLC and HPLC. All three species had remarkably different content and composition. However, within each species they were similar. Twelve major ginsenosides were determined in P. ginseng, eight in p. quinquefolium, and six in P. notoginseng. Of the components of P ginseng Rf, $Rh_1$, $Rh_2$ and Ra were not detected in P quinquefolium, and $Rb_2$, Rc, Rf, $Rh_2$, Ra and Ro not detected in P. notoinseam. Crude saponin content and protopanaxadiol/protopanaxatriol saponin ratio were compared. They were 4.81~5.24% and 1.27~ 1.45 in p. ginsengs, 7.01~7.25% and 2.12~ 2.15 in p. quinquefolium, 9.80% and 0.99 in P. notoineng. The prosapogenin and sapogenin content were different among the Panax species.

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Comparison of Saponin Content and Antioxidant Effect depending on the Processing Method of Codonopsis lanceolata

  • Kim, Eun Young;Jeon, Jeong Wook;Hyun, Kyung-Yae
    • Biomedical Science Letters
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    • v.27 no.4
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    • pp.291-297
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    • 2021
  • Codonopsis lanceolata, called deodeok in Korean, has been verified for various effects, including anti-aging and anti-inflammatory effects and insomnia improvement, and is one of the healthy foods that Koreans enjoy eating. In this study, the saponin content, lansemaside A content, and total saponin content of deodeok were analyzed using high-pressure sterilization and solid fermentation by mixed lactic acid bacteria. The antioxidant effect was compared to determine improved processing methods of deodeok. The lansemaside A content of deodeok samples depending on the preprocessing methods was analyzed: 2,594.10 mg/kg for dried deodeok, 2,100.93 mg/kg for steamed deodeok, and 1,151.31mg/kg for fermented deodeok. The total saponin content was found to be 7,209 mg/kg for dried deodeok and 8,605 mg/kg for steamed deodeok, showing a high saponin content. The total polyphenol content was highest for dried deodeok, steamed deodeok, and fermented deodeok. As for the antioxidant effect, it was analyzed that the effect of dried deodeok was the highest, followed by steamed deodeok and fermented deodeok; Dried deodeok had the highest value in total polyphenol content, not in total saponin content, which is considered to have a positive influence on its antioxidant effect. The content of lansemaside A was the highest for dried deodeok. When fermented deodeok is consumed, more beneficial effects on health can be expected by ingesting it with lactic acid bacteria cultured using saponins and polyphenols. Therefore, this study suggests the possibility of manufacturing products suitable for the needs of consumers, such as the flavor of deodeok, according to the processing methods.

Development of a Natural Surfactant from Extracts of Saponaria officinalis L. (비누풀 잎 추출물로부터 천연 계면활성제 개발)

  • Jang, A Reum;Kim, Hyo Jeong;Kim, Kwang Soo;Park, Eun Kyung
    • KSBB Journal
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    • v.28 no.3
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    • pp.202-207
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    • 2013
  • In this study, saponin content of extract from modified preconditioning process was investigated in Saponaria officinalis L. for cosmetic natural surfactant. Saponin content in steamed leaves from S. officinalis L. was about three times more than that in dried leaves (tea saponin and quillaja saponin). And saponin extracts from steamed leaves was excellently shown in both forming force and forming stability. In emulsion activity, saponin extracts from steamed leaves had a similar level to quillaja saponin and tea saponin. Saponin extracts from steamed leaves in S. officinalis L. showed nontoxic effect below in $1,000{\mu}g/mL$ of concentration and dose-dependent inhibition of NO production. From the experiment, the extracts of S. officinalis L. showed good cosmetic agent.

The Study on the Comparisions of Ingredients in Yam and Bitter Taste Material of African Yam (국내산 마의 성분비교 및 아프리카 마의 쓴맛 물질에 관한 연구)

  • 임선아;김영희;오승희;하태익;이만정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.1
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    • pp.74-81
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    • 1995
  • In the proximate composition of the yams of home product, wild yam was most abundant in crude lipid and crude protein, and those content in the cultivated yams, cultivated long yam and cultivated short yam were similar. Cultivated long yam is the richest in crude ash. African yam had more crude lipid and crude protein compare to those of home product yams. Especially, the amount of crude lipid in African yam was four-fold of home product yams. In the mineral content, Ca was the richest mineral in four kinds of yam. The rest of mineral were in order of Fe, Mn and Zn. Among the home product yams, the Ca content of wild yam was higher than that of cultivated yam. The contents of Fe, Mn and Zn were also high in wild yam compared to cultivated yam. In the composition of free sugars, the sucrose content is the highest in wild yam, cultivated short yam and African yam. in order The fructose content was the most abundant in cultivated long yam and the next was sucrose. The composition of free fatty acids were in order of lactobacillic acid, palmitic acid and palmitoleic acid. Crude saponin contents of the home products were almost similar levle, but crude saponin of wild yam was more abundant than that of cultivated yam. And crude saponin of African yam was much more abundant compared that of other home product yams. there is not much difference in the kinds of saponin from the home product yams. However, the amount of each saponin from the home product yams was different. In case of African yam, 'b' saponin did not appear and 'e' saponin was higher compared with home product yams. In African yam, the bitter taste from ethyl acetate layer was the strongest, and the next was n-butanol layer. It is possible that there was a powerful UV absorption material in the bitter taste of ethyl acetate layer and also supposed that there were saponin and phenol is material.

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Production of Saponin by Hairy Root Culture of Bupleurum falcatum L. I. Comparison of Saponin Content and Pattern in Callus, Adventitous Root, Hairy Root and Cultivated Root (시호(Bupleurum falcatum L.)의 모상근 배양을 통한 Saponin 생산 I. 캘러스, 부정근, 모상근과 재배근의 Saponin 양상과 함량 비교)

  • 안준철
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.43-49
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    • 1993
  • In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from these were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 $\mu$M 2, 4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes. A4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.

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Comparison of Phytochemical Constituents According to the Cultivation Method (Paddy Field Cultivation, Upland Field Cultivation) of Korean Fresh Ginseng (한국산 수삼의 재배환경(논, 밭)에 따른 식물화학성분 비교)

  • Her, Jae Young;Kim, Do Hyeong;Hwang, Yu Jin;Ko, Sung Kwon;Yang, Byung Wook
    • Journal of Korean Medicine for Obesity Research
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    • v.22 no.1
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    • pp.21-29
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    • 2022
  • Objectives: This study aimed to examine the changes in ingredients according to its cultivation method by examining the content of saponin and non-saponin components of ginseng. Methods: Ginseng saponin component analysis was compared and reviewed using the high-performance liquid chromatography method, and acidic polysaccharide component was measured using the carbazole sulfuric acid method. Results: The comparative analysis of ginseng saponin content of 4 and 6 years old fresh ginseng showed the following results. According to the cultivation method, upland field cultivation fresh ginseng showed higher average content of crude saponin than paddy field cultivation fresh ginseng. Whereas, paddy field cultivation fresh ginseng showed higher average content of total saponin than upland field cultivation fresh ginseng. Ginsenoside Rb1 showed higher content of paddy field cultivation fresh ginseng than upland field cultivation fresh ginseng in 6 years old ginseng. However, it showed higher content of upland field cultivation fresh ginseng than paddy field cultivation fresh ginseng in 4 years old ginseng. Additionally, ginsenoside Rg1 showed higher content of paddy field cultivation fresh ginseng than upland field cultivation fresh ginseng in 6 years old ginseng, whereas upland field cultivation fresh ginseng showed higher content of paddy field cultivation fresh ginseng in 4 years old ginseng. The effect on the content of ginseng saponins and acidic polysaccharides according to paddy field and upland field cultivation methods is considered to be small. Conclusions: The paddy field cultivation method, which is more efficient in production cost due to depletion of farmland and long-distance cultivation, is considered to be an economical cultivation method.