• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.630-635
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• 1998

Tobacco mosaic tobamovirus (TMV), cucumber mosaic cucumovirus (CMV), cucumber green mottle mosaic tobamovirus (CGMMV) and zucchini yellow mosaic potyvirus (ZYMV) from individual fruits and seeds of hot pepper and cucumber were detected by the reverse transcription-polymerase chain reaction (RT-PCR). The dilution end-points for RT-PCR in curde sap from TMV. and CMV - infected hot pepper leaves and CMV - and CGMMV-infected cucumber leaves were 10-5. However, the amount of PCR product obtained from preparation of ZYMV-infected cucumber leaf was 10-fold lower than those of CMV or CGMMV-infected cucumber leaves. In hot pepper, both TMV and CMV were detected in all parts of the fruit wall tissue, but the yields of PCR products in the fruit stalk and its surrounding tissues were higher than those of the end parts of the fruit. On the other hand, in cucumber fruit infected with CMV, CGMMV or ZYMV, the fruit wall tissue and seed located in both stalk and end parts showed higher yields of PCR products than those of intermediate parts. Of five viruses that were analysed, only TMV in hot pepper seed, and CGMMV and CMV in cucumber seed were detected in testa parts.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.625-629
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• 1998

Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-poly-merase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 nm in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV--specfic oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var. hebecarpa, Rorippa cantoniensis and Thlaspi arvense.

• Research in Plant Disease
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• v.21 no.3
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• pp.180-185
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• 2015

In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

• International Journal of Aeronautical and Space Sciences
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• v.6 no.2
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• pp.64-75
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• 2005

The Stratospheric Airship Platform (SAP) has a capability of performing the autonomous and guidance flight to satisfy given missions. To be used as the High Altitude Platforms (HAPs), the capabilities of controlling platform's accurate position and keeping the station point are the most important features. Under this circumstances Autonomous Flight Control System (AFCS) is a critical system and plays a key role in achieving the given requirements and succeeding in missions. In this paper, the design and analysis results of the AFCS algorithms and controller are presented. The brief summary of the AFCS hardware structure is also explained. The autopilot controller and guidance logics were designed based on the linear dynamics of the unmanned airship platform and the full nonlinear dynamics was considered to evaluate and verify their performances.

• The Korean Journal of Food And Nutrition
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• v.19 no.3
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• pp.267-270
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• 2006

In Korea, for a long time Rhus verniciflua has traditionally been used as an herbal medicines plants. A stem of Rhus verniciflua has been used to treat gastrointestinal trouble with in form of boiled chicken as a folk medicine. But it has been recognized as an extremely active allergen causing skin reactions. The chief allergenic component, urushiol, is found within the oleoresinous sap of Rhus verniciflua. Most components of urushiol have unsaturated side chains. These unsaturated side chains of urushiol are important to polymerization of these natural products. The urushiol components in Korean lacquer were isolated by reversed phase HPLC. The molecular weight of purified urushiol was determined as 340 from mass analysis. This compound was identified as Heptadecatetraenyl catechol (MW 340).

• Textile Coloration and Finishing
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• v.19 no.6
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• pp.1-6
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• 2007

Opuntia Ficus has been an epiphyte plant since it was introduced in Cheju island as an ornamental plant two hundred years ago. And now it is grown as many as it is designated to local souvenir No.35. The fruit of this plant contains red-purple sap, which is due to be used in coloring clothes. This study aims at confirmation of use possibility as eco-friendly dyeing stuffs on extract from opuntia ficus fruits and contributing to the practical use of natural dyeing. Thus this study was investigated the dyeability of extract from opuntia ficus fruits on the silk fabric. Antioxidants were used ascorbic acid, L-cysteine, prophyl gallete. After dyeing, K/S and dye fastness were measured. The results were as follows; Maximum absorption band of extract from opuntia ficus fruits was 534.5nm. The appropriate dyeing condition was bath ratio 50:1, dyeing temperature $30^{\circ}C$, dyeing concentration 50%, dyeing time 50min, pH 3,concentration of all antioxidants 3%. Addition of antioxidants brought increase of K/S value. And K/S value and dye fastness had the greatest value when ascorbic acid was added

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.4
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• pp.1-10
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• 2000

This study aims at the new design system development of landscape architecture structures, as soundproof wall using reinforced soil block. This structures, that is new soundproof wall system, have to be maintained stability on acting critical wind load, which is combined exist soundproof wall system and soundproof wall system using environmental green stone block. To the harmony of this system, the post block, so-called landscape block or cast block, is manufactured. It's possible to stand of the post bearing system combined with post-pile and post block. Through the comparison with a serious code for the acting wind load on the soundproof wall, the reasonable wind load could be calculated. Also, the mechanical stability on the green stone block was checked by the Lab. tests based on the UBC (Uniformed Building Code). Because the critical height of soundproof wall system using green stone generally was restricted, the new system demands to combination of the exist system and the new system. For the stability analysis of them, the utility program, SAP2000, was used. And, a semi-auto program on the design system of the new soundproof wall using green stone was developed, which can be easily use because of the simplification.

• Earthquakes and Structures
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• v.9 no.6
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• pp.1273-1290
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• 2015

JK wall is a shear wall made of lightweight EPS mortar and reinforced with a 3-D galvanized steel mesh, called JK panel, and truss-like stiffeners, called JK stiffeners. Earlier studies have shown that low strength lightweight concrete has the potential to be used in structural elements. In this study, seismic contribution of the JK infill walls surrounded by steel frames is numerically investigated. Adopting a hybrid numerical model, behavior envelop of the wall is derived from the general purpose finite element software, Abaqus. Obtained backbone would be implemented in the professional analytical software, SAP2000, in which through calibrated hysteretic parameters, cyclic behavior of the JK infill can be simulated. Through comparison with earlier experimental results, it turned out that the proposed hybrid modeling can simulate monotonic and cyclic behavior of JK walls with good accuracy. JK infills have a panel-type configuration which their dominant failure mode would be ductile in flexure. Finally technical and economical advantages of the proposed JK infills are assessed for two representative multistory buildings. It is revealed that JK infills can reduce maximum inter-story drifts as well as residual drifts at the expense of minor increase in the developed base shear.

• Earthquakes and Structures
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• v.10 no.6
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• pp.1331-1346
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• 2016

Historical masonry structures have an important value for cultures and it is essential for every society to strengthen them and confidently transfer to the future. For this reason, determination of the seismic earthquake response, which is the most affecting factor to cause the damage at these structures, gain more importance. In this paper, the seismic earthquake behaviour of Kaya Çelebi Mosque, which is located in Turkey and the restoration process has still continued after 2011 Van earthquake, is determined. Firstly the dynamic modal analysis and subsequently the seismic spectral analysis are performed using the finite element model of the mosque constructed with restoration drawings in SAP2000 program. Maximum displacements, tensile, compressive and shear stresses are obtained and presented with contours diagrams. Turkish Earthquake Code and its general technical specifications are considered to evaluate the structural responses. After the analyses, it is seen that the displacements and compressive/shear stresses within the code limits. However, tension stresses exceeded the maximum values at some local regions. For this mosque, this is in tolerance limits considering the whole structure. But, it can be said that the tension stresses is very important for this type of the structures, especially between the stone and mortar. So, some additional strengthening solutions considering the originality of historical structures may be applicable on maximum tensile regions.

• Journal of Plant Biology
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• v.15 no.1
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• pp.23-27
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• 1972

A total of 163 virus infected pepper plants(Capsicum annuum L.) collected from various pepper growing regions in Korea were investigated on the presence of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X(PVX), potato virus Y(PVY) and alfalfa mosaic virus (AMV) by serological methods. Van Slogteren's microprecipitin test was applied for the testing of TMV, PVX and PVY from infected plants, and Ouchterlony agar double diffusion test was used for CMV and AMV. Results obtained are as follows: 1. TMV, CMV, PVX, PVY and AMV were found to occur on the pepper plants growing in Korea. 2. The prevalence of each of these viruses among the 163 pepper plants investigated was in the order of CMV: 93 plants(57.0%)>TMV: 91 plants (55.8%)>AMV: 58 plants (35.6%)>PVY: 40 plants (24.5%)> PVX:6 plants(3.7%). 3. Among the 163 plants investigated, 72 plants (44%) showed infection with one kind of virus and 91 plants (56%) showed mixed infection with more than two different viruses. In general, heavier damage of the plants was observed from mixed infection. 4. The results of serological identification of pepper viruses coincided with those results obtained by sap inoculation experiment conducted at the Horticultural Experiment Station along with present investigation. Thus the serological techniques applied in this experiment proved to be very reliable for the identification of TMV, CMV, PVX, PVY and AMV from pepper plants infected with these viruses.