• Korean Journal of Soil Science and Fertilizer
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• v.4 no.1
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• pp.79-85
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• 1971

The rice variety, Kwanok, was reared in the water, land and salty beds and transplanted to the reclaimed soil area having an average of 0.48% salt concentration 0.67% at the end of April Two levels of $NH_3$-N and urea-N with 6 treatments were used. The effect of each treatment was observed. The plant height of the land bed seedlings at transplanting stage was short but the dry-weight/plant-height ratio was large, and the rooting ability vigorous remarkably after transplantation in the salty area. The total carbohydrate content of the stem part was markedly larger in the land bed seedlings than the others and the C/N ratio was accordingly greater. This tendency was observed through the last rooting stage. In the salty nutrient solution, the roots of salty bed seedlings showed high respiratory activity. The activity of the land bed seedlings did not decreased in the hypertonic solution as much as the water bed seedlings. There was no difference in the effect of fertilization on the rough rice yield between ammonium sulphate and urea. The cultural practices with the land and salty bed seedlings increased the rough rice yields by 33% and 22% respectively, compared with the yields of the water bed Seedlings. The number of panicles, panicle weight and the number of grains per panicle were much greater from the rice plant grown by the land bed seedlings than from the other bed seedlings.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.502-509
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• 2011

It is important to determine optimum soil covering depths for tree survival and growth because soil covering depths for establishing tree planting bases in coastal reclaimed lands are related to the costs for soil collection, transportation and land reclamation. The objectives of this study were carried out to determine optimum soil covering depths for the normal growth of planted trees in a coastal reclaimed land. The study sites were located in Asan National Industrial Complex in Pyeongtaek City, Gyeonggi-do. Four tree species (Pinus thunbergii, Chamaecyparis pisifera, Zelkova serrata, Quercus acutissima) with one hundred eighty trees of each species were planted in various depths of soil covering (no soil covering, 0.5 m, 1.5 m, 2.0 m soil covering treatments) on April 1998, and the tree growth patterns were measured on September 2000. The change of soil properties, tree mortality rate, root collar diameter and height growth were measured from each soil covering depth treatment on September 2000. Soil pH, EC, exchangeable cations ($K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$), anion $Cl^-$, and base saturation increased with decreased soil covering depths. The mortality rates of tree species showed decreased with increased soil covering depths. The height growth of tree species increased with increased soil covering depths. Height growth of Pinus thunbergii was significantly different between the soil covering depth below 0.5m and other three covering depths, while the growth of other species (C. pisifera, Z. serrata, Q. acutissima) was significantly higher in soil covering depths below 1.5 m than in other soil covering depth treatments. The root collar diameter growth of all tree species showed increasing trends with increased soil covering depths. It is recommended to cover the soil depths above 1.5 m to decrease mortality and to stimulate the tree growth of C. pisifera, Z. serrata and Q. acutissima, while P. thunbergii which is a salt tolerate species could be planted in the 1.0 m soil covering depth.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.6
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• pp.482-492
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• 2000

Vesicular arbuscular mycorrhizal (VAM) fungi are known to increase plant growth as well as to enhance salt tolerance of plants where plant roots are colonized by VAM. In pot experiment, pepper was grown in soil containing 0, 200, 400, and $600P\;kg\;ha^{-1}$ with and without mycorrhizal inoculum. Pots were irrigated with saline water containing 0.5, 2.0, and $6.0dS\;m^{-1}$. At 0, 200, and $400P\;kg\;ha^{-1}$ of three EC treatments, plant hight in mycorrhizal treatments was significantly different compared to nonmycorrhizal treatments. However, plant hight at $600P\;kg\;ha^{-1}$ was not different between mycorrhizal and nomycorrhizal treatments. Leaf area at $0P\;kg\;ha^{-1}$ of three EC treatments, and $200P\;kg\;ha^{-1}$ of $6.0dS\;m^{-1}$ in mycorrhizal treatments significantly increased compared to nonmycorrhizal treatments. However, these increase were not discovered in high salinity and P level. Level of EC affected dry weight, and especially, interection of P and EC, or P and VA inoculation highly affected root dry weight. R/S ratio generally decreased in mycorrhizal treatments. Significantly decreased R/S ratio was shown at 0, 400, and $600P\;kg\;ha^{-1}$ of $6.0dS\;m^{-1}$. Chlorophyll content generally increased with decreased salinity and P level where mycorrhizal treatments showed higher chlorophyll content compared to nonmycorrhizal treatments. The benefits of VAM inoculation on fruit production was discovered at only low P level and salinity. Mycorrhizal dependency on dry weight basis was generally shown in $0P\;kg\;ha^{-1}$ of three EC treatments and 0.5, $2.0dS\;m^{-1}$ of $200P\;kg\;ha^{-1}$ level. Colonization rate ranged 3.3 to 43.3% and number of spores was 47.7 to 198.3 $100g^{-1}$ soil. Colonization rate and number of spores increased with decreased P level and salinity where there was high correlation ($r=0.858^{**}$) between both. Also improved uptake of mineral nutrients was discovered at mycorrhizal treatments in decreased P level and salinity.

• Journal of Life Science
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• v.28 no.7
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• pp.835-840
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• 2018

Quantitative determination of the protein expression levels is one of the most important parts in assessment of the safety of foods derived from genetically modified (GM) crops. Overexpression of AtCYP78A7, a gene encoding cytochrome P450 protein, has been reported to improve tolerance to abiotic stress, such as drought and salt stress, in transgenic rice (Oryza sativa L.). In the present study, an enzyme-linked immunosorbent assay (ELISA) kit for diagnosing AtCYP78A7 protein including AtCYP78A7-specific monoclonal antibody was developed. GST-AtCYP78A7 recombinant protein was induced and purified by affinity column. Four monoclonal antibodies (mAb 6A7, mAb 4C2, mAb 11H6, and mAb 7E8) against recombinant protein were also produced and biotinylated with avidin-HRP. After pairing test using GST-AtCYP78A7 protein and lysate of rice samples, mAb 4C2 and mAb 7E8 were selected as a capture antibody and a detecting antibody, respectively, for ELISA kit. Product test using rice samples indicated that percentages of detected protein in total protein were greater than 0.1% in AtCYP78A7-overexpressing transgenic rice (Line 10B-5 and 18A-4), whereas those in negative control non-transgenic rice (Ilpum and Hwayoung) were less than 0.1%. The ELISA kit developed in this study can be useful for the rapid detection and safety assessment of transgenic rice overexpressing AtCYP78A7.

• Korean Journal of Food Science and Technology
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• v.26 no.4
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• pp.382-388
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• 1994

Because of its restrictive harvest from spring to summer, It is necessary to preserve raw ascidians, Halocynthia roretzi, for the purpose of processing regardless of season. We evaluated low Temperature tolerance of ascidian and conditions for cold storage to secure the quality of the stock. To retard the browning of meat rapidly occurred after sucking, ascidians were blanched for 10 seconds in 10% boiling salt solution or dipped for 60 seconds in 0.2% $NaHSO_3$ solution, respectively. The samples were stored in ice, at $-17^{\circ}C$ or $-35^{\circ}C$ for 85 days, respectively. Changes in VBN, glycogen, brown pigment formation, total carotenoid, nucleotides and their related compounds during the storage were determined, and sensory evaluation of quality was also practiced. VBN and brown pigment formation were rapidly increased. Glycogen was gradually decreased and then not detectable after 85 days in case of ice storage. Lipophilic brown pigment was higher than hydrophilic and rapidly increased during storage. The result of sensory evaluation showed that the ascidian treated in 0.2% $NaHSO_3$ was good for 85 days of storage at $-35^{\circ}C$ . Judging from the results of chemical experiment and sensory evaluation, the quality of ascidian treated in 0.2% $NaHSO_3$ and stored at $-35^{\circ}C$ was better than that of other samples.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.1
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• pp.16-24
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• 2010

Effect of flooding on the desalting efficiency and the growth of sudan grass, barnyard grass, sesbania and corn was studied in a sandy soil of the Iweon reclaimed tidal land. Flooding plots were treated by 400 (one time flooding), 800 (two times flooding), and 1,200 mm(three times flooding) of water, respectively, and then soil salinities of the treated plots were compared with salinity of the control plot (not flooded) for estimation of desalting effect. Desalting ratio of 1,200 mm treatment was 78.3% for depth 0-20 cm, 70.5% for depth 20-40 cm and 60.8% for depth 40-60 cm, and then the soil salinity reached at 3~6 dS $m^{-1}$. Consequently, it was considered that sandy saline soil was satisfactorily desalted for upland crops to be cultivated by 1,200 mm flooding, but insufficiently desalted by 400 mm and 800 mm flooding because of high salinity ranged 5~14 dS $m^{-1}$ even after flooding treatment. In addition, it was estimated that soil salinity should be controled lower than 7.7 dS $m^{-1}$ in order to obtain more than 80%of crop emergence when four crops are simultaneously cultivated by inter- or mixed cropping in a field. Dry matter yields (kg $10a^{-1}$) was 1,068 for sudan grass, 696for barnyard grass, 1,426 for sesbania, and 1,164 for corn by 1,200 mm flooding treatment, but only 46.8~74.3% by 800 mm flooding treatment and 2.9~25.5% by 400 mm flooding treatment. Therefore, it is concluded that the flooding treatment more than 1,200 mm is necessary for satisfactory desalinization in order for the low salt tolerance crops to be cultivated in the sandy reclaimed tidal land.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.53-58
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• 1988

This study was carried out to determine effect of butachlor [N.-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide] and bialaphos [2-amino-4(hydroxy)(methyl) phosphionyl] butyryl-alanylalanine sodium salt on the germination of tobacco seed, induction and growth of callus from tobacco. Further, fatty acids and ammonia content of tobacco calli were determined. Bialaphos had no effect on tobacco seed germination, but the growth of seedling was markedly affected by an application of 10 ppm bialaphos. However, regardless of varieties tested, tobacco seed germination was completely inhibited by $5{\times}10^{-5}M$ of butachlor. At an application of $5{\times}10^{-5}M$ butachlor, tobacco seeds were to some extent germinated and showed further growth. Hyangcho among varieties tested, showed the most tolerant response to butachlor. In induction of callus from various tobacco varieties and their growth, aromatic type of tobacco varieties exhibited the most tolerance against bialaphos. However, no distinct varietal differences were determined in the treatment of butachlor. The major fatty acids identified in tobacco calli were palmitic, oleic and linoleic acid. No marked difference in terms of fatty acids was observed among tobacco varieties used, but it was observed that there was the higher ratio of quantity in unsaturated fatty acids over saturated one, bialaphos treatment accumulated about 9 times higher ammonia content than that of the untreated control, giving an evidence that bialaphos might inhibit glutamine synthetase activity.

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.35-41
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• 1970

The experiment was conducted at the salt concentration of 0.5% and 1% end of April, respectively, in low and high-salty and the non-salty areas of silt loam with the Nongkwang, rice variety. The factorial design with confounding blocks of 3 levels each of 10, 15 and 20 kg of N, 8, 12 and 16kg of phosphate and potash, respectively, per 10a was applied. 1. N applications increased by 1.5 and 2 times with the fixed amount of $P_2O_5$ and $K_2O$ (8kg/10a each) increased the proportion absorbed to the applications of N in both non salty and low-salty areas. It was observed that the absorption of Ca and Si was inhibited by either an increased treatment of N alone or combination with the other nutrients in the salty area. 2. In the non-salty area, an increased applications of standard amount of N, $P_2O_5$ and $K_2O$ respectively did not increased the yields. Doubling the application of $K_2O$ resulted in a decreased yield. 3. Applications of additional of 1.5 and 2 times the 10 kg of N per 10a increased the rice yields 12% and 21% respectively, in the low-salty area. An increased application of $P_2O_5$ and $K_2O$ failed to bring about an increased yield. 4. Increasing the application of N gave a significant increased in the yield of rice grain and 1.5 times of N applications were seemed profitable on the high-salty area. Although an increased applications $P_2O_5$ and $K_2O$ seemed to increase the yields of grain, no significant increase was observed. 5. An increased application of N increased the number of panicles up to 1.5 times the standard amount in the non-salty area, but no further increase resulted by doubling the application. The number of panicles was increased in proportion to the increased application of N in both low and high-salty areas. An increased application of $P_2O_5$ increase the number of panicles per unit area in each experimental plot while that of $K_2O$ had no effect but rather decreased the number. 6. The effect of an increased application of N decreased the weight of panicle in the non-salty area, but when the application was increased to 1.5 times or more an increased weight of panicle resulted in both salty areas. Doubling the application had approximately the same effect as 1.5 times the application. Increasing the applications of $P_2O_5$ and $K_2O$ had no effect on the panicle weight in the experimental plots. Increasing the applications of N, $P_2O_5$ and $K_2O$ did not effect the weight of 1,000 grains produced in the non-salty and salty areas. Increasing the application of N decreased the number of grains per panicle in the non-salty area but increased the number of grains per panicle in either salty areas. 7. The ratio of matured grains was highest in the low-salty area and the lowest in the high-salty area. An increased N applications decreased the ratio of matured grains in the non-salty area. No effect was observed in both low and high-salty areas. Increased the $P_2O_5$ and $K_2O$ application showed no effect on the ratio of matured grains in the experimental plots. 8. Increased applications of N, $P_2O_5$ and $K_2O$ was observed not to change the percentage of milling recovery in any experimental plots. Broken rice was increased equally by an increased application of N in the non-salty and salty areas but more remarkably so in the former. 9. Increased applications of N increased the straw production equally in the non-salty, low and high-salty areas. However, no increased production was observed from heavier applications of $P_2O_5$ and $K_2O$. Additional N applications reduced the rate of rough grain weight v.s. straw weight in the non-salty area but increased the ratios in both low and high-salty areas. Additional $P_2O_5$ and $K_2O$ had no effect with the ratio.