• Title/Summary/Keyword: Salt form

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Detection and Genomic Analysis of Viroid-like RNA Molecules Isolated from Korean Peonies (한국산 작약에서 분리한 바이로이드 유사 RNA 분자의 확인과 유전자 분석)

  • 정동수;김무인;이재열
    • Korean Journal Plant Pathology
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    • v.13 no.2
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    • pp.113-117
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    • 1997
  • Low moleuclar weight (LMW) RNAs were isolated form Korean peonies which expressed symptoms of stunt and epinasty. The LMW plant RNAs were purified by Qiagen column chromatography which could separate viroid specific nucleic acid at differential salt concentration. After the inoculation of the purified RNAs from the peonies, the inoculated tomatoes (cv. Rutgers) expressed the symptoms of stunt and epinasty. Also the same molecular weight RNAs with viroid-like RNAs were isolated from the inoculated tomatoes. Double-stranded cDNA were synthesized by the methods of reverse transcription (RT) and polymerase chain reaction (PCR) with the purified RNA and primers. The same cDNAs associated with viroid-like RNAs wre cloned from the inoculated tomatoes. The cDNA has been sequenced and its 375-nucleotides were arranged into secondary structure. The cloned cDNA showed 47~54% homology compared with other viroids. The sequence homology of the cloned cDNA were partially high with plant genomic RNAs.

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Kimchi Fermentability of the Spring Chinese Cabbage (봄배추 품종별(品種別) 김치가공적성(加工適性))

  • Chun, Jae-Kun
    • Applied Biological Chemistry
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    • v.24 no.3
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    • pp.194-199
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    • 1981
  • In order to compare Kimchi fermentability of Chinese cabbage cultivars, five cultivars grown in Korea were fermented in 3% NaCl. The measurements were done for both the fermenting properties: bacterial growth, $CO_2$ generation, acid production, dissolved oxygen, pH changes in the fermenting broth and extractable sugars form the cabbage tissue and salt solution, respectively. The measured values were scorized to evaluate the cultivar's fitness to Kimchi processing. The most rapid cultivar is Naeseu Baekno and the lowest one is Mussang.

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Polyaniline Prepared by One-step Emulsion Polymerization and Its Conducting Blends (원-스텝 에멀젼 중합법으로 제조된 폴리아닐린과 이를 이용한 전도성 블렌드)

  • 이보현;김태영;김종은;서광석
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.15 no.2
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    • pp.153-161
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    • 2002
  • Stable polyaniline-dodecylbenzenesulfonic acid(PANI-DBSA) fully dissolved in toluene was obtained by a direct one-step emulsion polymerization technique. By using the proper molar ratio of APS/aniline monomer and DBSA/aniline monomer, the highest conductivity(7 S/cm) of PANI was obtained. The UV-Vis absorption spectrum of PANI confirmed PANI is emeraldine salt form. PANI/styrene polymers (polystyrene and styrene-butadiene copolymer) blends were prepared by mixing PANI solution with polymers in toluene. These blends exhibited the conductivity of 10$\^$-4/-10$\^$-3/ S/cm at 1 wt. % of PANI content. The mechanical property of conducting blend was decreased and TGA thermograms of conducting blends were similar to that of PANI. It had been checked that the flatness of coating layers of conducting blends decreased with increasing conducting components. It was also found that the morphology of blends was setting closer to that of PANI at higher conducting component contents.

Accelerating Effect of Squid Viscera on the Fermentation of Alaska pollack Scrap Sauce

  • Kim, Sang-Moo
    • Preventive Nutrition and Food Science
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    • v.4 no.2
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    • pp.103-106
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    • 1999
  • Fish sauce is a liquid form of salt-fermented fish and has played an important role in Korean dietary life. Fish sauce was manufactured by utilizing Alaska pollack scrap from Himedara(seasoned and dried Alaska pollack tail) processing . In addition, the effects of squid viscera as a fermentation enhancer were also evaluate.Ph of Alaska plllack scrap sauce with squid viscera was lower than that of control over the entire fermentation process. Squid viscera acceleraged the production of amino-nitrogen, VBN , TBA and free amino acids, and the degradation of IMP and Inosine. The addition of squid viscera and koji at 5% concentration, respectively , also accelerated the digestion of Alaska pollack scrap and was similar to the results of squid viscera at 10% concentration.

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On the Micellization of ${\alpha}$-Amino Capric Acid (${\alpha}$-아미노카프르산의 Micelle 형성을 위한 한계농도에 관하여)

  • Ryu, Ji-Yong;Chang, Sei-Hun
    • Journal of the Korean Chemical Society
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    • v.10 no.4
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    • pp.161-165
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    • 1966
  • The critical concentrations of ${\alpha}$-amino capric acid for micelle formation have been determined by the surface tension measurements in both acidic and alkaline solutions, and also by the dye titration using Rhodamine B in alkaline solutions. The critical micelle concentrations obtained by the two methods show the good agreements within experimental errors. Since ${\alpha}$-amino acid is an ampholyte, it may aggregate to form the micelles in both more acidic and basic media than its isoelectric point. It is found that the basic media are rather preferable for the micelle formation than the acidic media. The effect of gegen ions upon the critical concentration for micelle formation in alkaline media is similar to that expected from the salt effect on the CMC (Critical Micelle Concentration).

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Synthesis and In Vitro Antibacterial Activity of Quaternary Ammonium Cephalosporin Derivatives Bearing Oxazolidinone Moiety

  • Chung, In-Hwa;Kim, Choong-Sup;Seo , Jae-Hong;Chung, Bong-Young
    • Archives of Pharmacal Research
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    • v.22 no.6
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    • pp.579-584
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    • 1999
  • Several oxazolidinones having amine moiety were prepared to form a quaternay ammonium salt with cephalosporin nucleus, and antibacterial activity of the quaternary ammonium cephalosporin derivatives bearing oxazolidinone moiety were examined particularly with expectation of dual activity. However, the cephalosporin-oxazolidinone compounds revealed rather weaker antibacterial activity in vitro than their parent oxazolidinone and cephalosporin without showing any characteristic activity as expected.

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Properties of $\beta$-1,3-glucan Synthase System in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 베타-1,3-글루칸 합성효소 체계의 특성)

  • Park, Hee-Moon;Kim, Jeong-Yoon;Kim, Sung-Uk;Bok, Song-Hae
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.316-321
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    • 1995
  • Some properties of $\beta$-1, 3-glucan synthase system in Saccharamyces cerevisiae were investigated. By extraction with detergent and salt, the membrane preparations could be dissociated into two components, one soluble, the other still membrane bound. Both components, in addition to GTP, were necessary for the activity of $\beta$-1, 3-glucan synthase like other fungi. The protective effect of guanosine nucleotides on the soluble factor pointed to the possibility that this fraction contained a GTP-binding protein. Addition of increasing amounts of soluble factor to a constant amount of insoluble catalytic factor, vice versa, gave rise to a saturation curve. These results, including different types of evidence, indicate that the soluble factor and the catalytic factor form a complex.

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Monitoring of Agriculture land in Egypt using NOAA-AVHRR and SPOT Vegetation data

  • Shalaby, A.;Ghar, M. Aboel;Tateishi, R.
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.18-20
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    • 2003
  • Land cover change detection is one of the most important trends in which remote sensing data could be used to assist strategists and the planners to decide the best land use policy. Two images of NOAA-AVHRR and SPOT vegetation acquired in November 1992 and 2002 were used to assess the changes of Agricultural lands in Egypt. A supervised classification together with two change images derived from classification result and NDVI were used to evaluate the trend and form of the change. It was found that agricultural areas increased by about 14.3 % during the study period in particular around the River Nile Delta and near the Northern Lakes of Egypt. The new cultivated lands were extracted mainly from the desert and from the salt marches areas. At the same time, parts of the agricultural lands were turned into non-cultivated land because of the urban expansion and soil degradation.

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Aggregation and Dissolution of Cationic Dyes with an Anionic Surfactant

  • Park, Joon-Woo;Chung, He-SSon
    • Bulletin of the Korean Chemical Society
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    • v.7 no.2
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    • pp.113-116
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    • 1986
  • Spectral behaviors of cationic dyes, methylene blue(MB) and acridine orange(AO), with varying concentrations of sodium dodecylsulfate(SDS) were studied. At low concentration of SDS(<1mM), these dyes formed insoluble dye-surfactant aggregates. When [SDS] is 4-5 mM, the aggregates were dissolved into mixed micelles of constant composition. At higher concentration of [SDS], the composition of mixed micelles were changed with [SDS], resulting only monomeric form of dyes in micelles. AO-SDS system showed greater aggregating and less dissolving properties, and weaker effect of salt than MB-SDS system did. These were attributed to the greater hydrophobic nature of the former dye. The monomer/dimer ratios of dyes in mixed micelles at given [SDS] were greatest at $20^{\circ}C$, reflecting the dependency of CMC of the surfactant on temperature.

Vibrational Analysis of Dopamine Neutral Bae based on Density Functional Force Field

  • Park, Seon Gyeong;Lee, Nam Su;Lee, Sang Ho
    • Bulletin of the Korean Chemical Society
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    • v.21 no.10
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    • pp.1035-1038
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    • 2000
  • Vibrational properties of dopamine neutral species in powder state have been studied by means of the normal mode analysis based on the force constants obtained from the density functional calculation at B3LYP level and the results of Fourier trans form Raman and infrared spectroscopic measurements. Ab initio calculation at MP2 level shows that the trans conformer of dopamine has higher electronic energy about 1.4 kcal/mol than those of the gauche+ and the gauche-conformers, and two gauche conformers have almost the same energies. Free energies calculated at HF and B3LYP levels show very similar values for three conformers within 0.3 kcal/mol. Empirical force field has been constructed from force constants of three conformers, and refined upon ex-perimental Raman spectrum of dopamine to rigorous values. The major species of dopamine neutral base in the powder state is considered a trans conformer as shown in the crystallographic study of dopamine cationic salt.