• 대한한의학회지
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• 제25권3호
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• pp.160-168
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• 2004

Background and Objectives: Tinnitus is on the increase due to the increase in the elderly population, industrial pollution and noise pollution. This symptom is especially marked in patients with a hearing problem and the relationship between cause, mechanism and treatment is poorly understood. The characteristics of tinnitus and other hearing problems are well brought out using an animal model with salicylate ototoxicity. Therapeutic effects of Uncariae Ramulis and Testudinis Plastrum were expected in tinnitus and hearing problems; therefore we experimented on an animal model with salicylate ototoxicity. Salicylate is one of the most commonly prescribed drugs, although it has been recognized that salicylate induces hearing loss and tinnitus reversibly. The purpose of this study was to find the therapeutic effects of this by the morphologic study using salicylate ototoxicity. Materials and Methods : Twelve healthy Sprague-Dawley rats were divided into three groups: normal, control and sample. The sample group was treated with the extract of Uncariae Ramulis and Testudinis Plastrum (1cc/100g, once a day for 6 days). Then, to induce the salicylate ototoxicity in the control and sample groups, rats were injected intraperitoneally with sodium salicylate (500mg/kg). We observed the morphologic changes in the cochlea of the rats every 2, 3, 4 and 5 hours after injection. Results : The outer hair cells showed marked changes. Vacuolization formed in the cuticular plate and the endoplasm of the control group. The endoplasm and the cuticular plates of the sample group after 2 hours were similar to the control group, but the cuticular plates of the sample group observed after 3, 4 and 5 hours were not similar. Conclusions : The results suggest that an extract of Uncariae Ramulis and Testudinis Plastrum has therapeutic effects on an animal model with salicylate ototoxicity.

• 한방안이비인후피부과학회지
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• 제18권1호
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• pp.221-233
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• 2005

Background and Objectives : Despite of high prevalence, Tinnitus remains obscure because the cause and mechanism is poorly understood. In the absence of a suitable animal model, past investigations tool place in humans. Recently, the animal model with sodium salicylate ototoxicity is broadly used because of its reversibility. Balyangtongum-Tang had been used clinically to treat tinnitus and other hearing problems. We investigated the effects of Balyangtongum-Tang on cochlear morphologic change induced by sodium salicylate ototoxicity and were to find out its therapeutic effects on ototoxicity in rat model. Materials and Methods : Healthy ten Sprague-Dawley rats were divided into normal(2), control(4) and sample(4) groups. The sample group was given extract of Balyangtongum - Tang(1cc/100g) once a day for 4 days. After 3 hours when last medication were given, the sample and control groups were injected intraperitoneally with sodium salicylate(500mg/kg). We observed the cochlear morphologic changes of rats every 1, 2, 3 and 5 hours after injection. Results : The electron microscopic finding of outer hair cell shows some changes in the curticular plate and cytoplasm. Some vacuoles were found in the control and sample groups. Vacuolization in the curticular plate and cytoplasm of the sample group after 3, 5 hours were similar to the control group. But the curticular plate of the sample group after 1, 2 hours did not from vacuole. On the other hand the control group after 1, 2 hours formed vacuoles in the curticular plate. Light microscopic findings of cochlear duct in control and sample groups didn't find any difference. Conclusion : The results suggest that extract of Balyangtongum-Tang reduces the morphologic changes induced by sodium salicylate ototoxicity and the effects are remarkable in frist 2 hours. However the effective times are different with previous studies, it seems to due to the difference of tolerance and sensitivity of laboratory animals.

• Applied Microscopy
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• 제18권1호
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• pp.92-102
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• 1988

살리실산이 백서 와우의 나선신경절의 미세구조에 미치는 영향을 전자현미경으로 관찰하였다. 생후 $4{\sim}5$주의 Sprague-Dawley계 백서에 $500{\sim}600mg/kg$의 용량으로 살리실산 나트륨을 7일간 피하주사한 후에 각각 24시간(1군), 6주(2군), 10주(3군) 경과 후에 와우조직을 얻어 나선신경절에 나타나는 미세구조의 변화를 관찰하였다. 1. 1군에서는 신경절세포와 위성세포 그리고 Schwann cell의 세포질내에 다양한 크기의 막소구의 팽창이 나타났으며 Schwann cell 내에서는 미토콘드리아가 팽윤되거나 다낭성 세포소체를 형성하였다. 2. 2군에서는 신경절세포나 위성세포내의 막소구들이 더욱 팽창하고 그 일부는 융합하여 세포질이 감소하였다. Type I 세포의 신경 세포체나 신경섬유 주위를 싸고 있는 수초는 각층 사이의 거리가 멀어지거나 일부는 소실되었고, Type II 세포내에는 신경미섬유가 증가하였다. 3. 3군에서는 수초의 변형 및 파괴로 신경절세포의 세포질 이탈이 관찰되었고 Corti기 및 골성 나선판에 위치하는 신경섬유들의 소실이 관찰되었다. 이상의 결과를 종합하면 나선신경절세포와 그 주위를 둘러싸는 위성세포 및 Schwann cell은 과량의 살리실산 나트륨 투여후에 미세구조의 변화를 보였으며 1군에서 2군, 3군으로 갈수록 변화가 더욱 현저하였다.