• Korean Journal of Microbiology
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• v.25 no.4
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• pp.282-289
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• 1987

The characteristics of the plasmid pKU10 isolated from Pseudomonas putida KU816 were investigated and its restriction map was constructed. The pKU10 plasmid was a small R plasmid carrying genes for resistance to ampicillin, tetracyclin, and chloramphenicol, and cured by treatment with mitomycin C. The molecular size of pKU10 was estimated to be 9.4Kb. Pseudomonas strains and E. coli cells could be transformed for antibiotic resistance characters specified by pKU10 plasmid DNA. By incompatibility test with other plasmids, pKU10 is grouped into IncP-1. EcoRI, XhoI, SalI, BglII, and SmaI cleaved pKU10 once, while PstI cleaved at two sites, and HindIII cleaved at six sites. The restriction map was constructed by partial and complete digestion of the purified plasmid DNA with single, double, or triple restriction enzymes. Thus, pKU10 is expected to be used for a cloning vector in Pseudomonas cells.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.21 no.1
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• pp.53-66
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• 1995

The gene for ${\gamma}$-casein, a milk protein, is a member of a family of casein gene which is expressed in mammary glands of the animal during the late gestation and lactation periods binder the influence of various hormones. In order to elucidate tile mechanisms b)'which hormones regulate the coordinate induction of milk protein genes, the mouse ${\gamma}$-casein gene was isolated and characterized. The ${\gamma}$-casein gene was screened from a mouse genomic library constructed in bacteriophage EMBL3 with the ${\gamma}$-casein CDNA used as probe and one clone was obtained. The ${\gamma}$-casein CDNA as probe was partially sequenced and contained ATG start codon and 5'-noncoding region. The cloned genomic DNA was digested with Sal I restriction enzyme, by which the insert DNA can be isolated from EMBL3 vector. Three DNA bands were observed and the size of DNAs was approximately 28kb, 14kb and 9Kb, respectively Accordingly the size of the insert DNA was calculated with approximately 23Kb. The result of Southern blot analysis, however, showed that the cloned genomic DNA was not hybridized with the synthetic oligonucleotides (40 mer) of cDNA 5'-end region, but it was hybridized with the y -casein CDNA. This means that tile cloned y -casein gene may not contain its promoter region. The ${\gamma}$ -casein genomic DNA containing the promoter region has been screening from mouse genomic library with oligonucleotides of CDNA 5'-end region as probe, and twenty-nine clones was obtained.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.264-270
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• 1994

A gene coding for a novel putative $\sigma$ factor of RNA polymerase has been identified from Streptomyces coelicolor A3(2) using Escherichia coli rpoS gene fragment as a probe. The 486 bp rpoS gene fragment was amplified from E. coli genomic DNA by PCR with two synthetic oligonucleotides, the sequences of which were deduced from the amino acid sequences in the regions 2.3 and 4.2 conserved among various bacterial factors. When E. coli genomic DNA fragments were hybridized with cloned rpoS probe, only one band corresponding to rpoS gene (3.2 kb PvuII fragment or 2.3 kb KpnI fragment) was detected. In S. coelicolor, however, two bands were detected both in PvuII digested DNA and SalI digested DNA. 3.5 kb PvuII fragment which binds the rpoS gene probe was cloned (pMS1) from the sublibrary, and the nucleotide sequences of 1.0 kb BamH'/HincII subclone (pBH2) was partially determined. The nucleotide sequences revealed extensive similarity to other $\sigma$ factor genes of S. coelicolor (hrdA, hrdB, hrdC, hrdD), S. aureofaciens (hrdA, hrdB, hrdC, hrdD), Synechococcus species, Pseudomonas aeruginosa, Stigmatella aurantiaca, and Anabaena species. The nucleotide sequences in regions 1.2 and 4 were compared with the corresponding regions of 5 known ${\sigma}$ factor genes of S. coelicolor by multiple alignment. It turned out that the cloned gene is most closely related to hrdA showing 88% amino acid similarity in region 1.2 and 75% in region 4.

• Journal of Microbiology
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• v.39 no.4
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• pp.338-342
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• 2001

Pseudomonas fluorescens SM11 is a naphthalene-degrading strain whose dissimilatory genes are cho-mosomally encoded. We have cloned the 2.9 kb Sal I fragment harboring genes for the naphthalene-degrading upper pathway. The nucleotide sequences were determined to be nahQ, napA, and partial regions of nahE genes. The nahQ encods a protein of 188 amino acid residues with a deduced molec-ular wight of 20.8kDa. The high homology with other proteins suggests that NAhQ may be an active and useful protein whtich gives as selective advantage to naphthalene degradatin. Transposase(TnpA)encodes a polypetide chain with a molecular mass of 41.8kDa consisting of 376 amino acid residues. The deduced anino acid sequence of tnpA revealed 96% idenitity with putative transposase of P. stutzeri OX1,. It was assumed that transposase plays an important role in the evloution of the catabloic-path way in the regulation of nah expression.

• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.18-22
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• 1994

Enterococcus faecalis KBL703 has three plasmids(p703/9, p703/5 and p703/4). Within p703/5, the specific DNA region that would confer replication function(replication origin) was searched by transformation experiments. In order to use as the recipient of transformation, two plasmid-cured strainsd were made from this strain. Four recombinant DNA constructs, each containing fragment of p703/5 and CAT(chloramphenicol acetyl transferase) gene were also made. And they were used to transform the plasmid-cured strains. Only one DNA construct containing 3.6 kb SalI fragment was stably maintained as plasmid in these strains. Additional experiment using another Enterococcus faecalis strain(ATCC29212) as a recipient was successfully done and it was confirmed that this newly constructed recombinant plasmid plasimid contained the replication origin from p703/5 plamid.

• Herbal Formula Science
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• v.9 no.1
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• pp.83-103
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• 2001

I studied conductant drug(使藥) in a prescription. As results of these studies, conductant drug(使藥) with meaning of medicinal guide(引經) were Achyranthis Bidentatae Radix(牛膝), Platycodi Radix(桔梗), Cinnabaris(朱砂), Sal Maritimus(食鹽), alcoholic drink(酒,) Cimicifugae Rhizoma(升麻) and Bupleuri Radix(柴胡). Conductant drugs with meaning of harmonizing medicines in a prescription were almost Glycyrrhizae Radix(甘草). And sometimes Mel(蜂蜜) was used with meaning of harmonizing medicines in pill. And Jujubae Fructus was used with meaning of harmonizing medicines in purgation medicines.

• Proceedings of the Safety Management and Science Conference
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• 2002.05a
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• pp.151-159
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• 2002

Real-Time Linux를 이용하여 실시간 운영체제가 요구하는 특성과 요구조건을 분석하고 이러한 요구조건에 부합하도록 리눅스를 하드웨어에 이식하고 하드웨어에 이식하기 위한 방법을 제시하였다. 다른 상용 실시간 운영체제 (RTOS)와는 달리 리눅스는 특정 하드웨어를 지원하기 위한 별도의 개발환경을 제공하지 않는다. 이에 개발환경을 구축하고 부트로더를 개발하기 위해 목표 시스템에 부합하도록 리눅스 커널을 이식하였다. 또한 응용 개발의 유연성을 제공하기 위하여 램디스크를 이용한 파일 시스템을 지원하도록 하였으며, GPIO(general purpose I/O)를 통한 디바이스 드라아버를 제작하는 등의 실험을 통해 시스템의 안정성을 검증하였다 실험에서는 StrongArm SAl110 마이크로프로세서를 이용하였으며 이 실험을 통해 실시간 운영체제로서의 리눅스의 활용 가능성을 확인하였다.

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.203-208
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• 1991

The dapA-complementing gene (L-2, 3-dihydrodipicolinate synthetase: DHDP synthetase, dapA) has been cloned by using a cosmid genomic bank of Corynebacterium glutamicum JS231 that is a lysine overproducer, AEC (s-(2-aminoethyl)-L-cysteine) resistant mutant. By enzymatic deletion analysis, the DNA region complementing the escherichia coli dapA host could be confined to 4.5kb SalI-generated DNA fragment. This DNA fragment was inserted into the C. glutamicum/E. coli shuttle vector pECCG117 to construct pDHDP5812. The specific activity of DHDP synthetase detected in C. glutamicum JS231/pDHDP5812 was increased about 10 fold above that of C. glutamicum JS231. The addition of leucine during growth did not repress the expressin of dapA, and the enzyme activity was not inhibited by lysine.

• Korean Journal of Plant Taxonomy
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• v.51 no.2
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• pp.166-170
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• 2021

Distribution of Viola inconspicua is newly discovered in Korea. This species was collected from grassy fields and roadsides in Jeju-si and Seogwipo-si, Jejudo Island. V. inconspicua shares several characteristics (i.e., purple or pale purple flowers, triangular leaf blades, winged petioles, and a linear-lanceolate stipules) with the related species V. mandshurica and V. yedoensis. However, V. inconspicua can be easily distinguished from other species by the following characteristics: leaf base (cordate vs. truncate to attenuate), and spur (short, 2-4 mm long vs. longer, 5-8 mm long). Here, we describe and illustrate of V. inconspicua. In addition, the identification key to allied species, photographs in its habitat, distribution, ecology, and phenology are provided as well. The Korean name for the species is given as 'Hwa-sal-ip-je-bi-kkot', considering the shape of its leaves.

• Journal of the Korea Furniture Society
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• v.28 no.2
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• pp.126-134
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• 2017

This study explores PyeongSang, a piece of traditional Korean furniture, in order to make people know the details about what the piece is in the first paper. And based on the paper, PyeongSang will be developed as moulded multi-useful beds and sofas adapting the joint and ornament from the structure and pattern of the piece in the next paper. As the result, it appeared as two styles such as bed types mentioned as SalPyeongSang and ttulmaru in Korean. The pieces have been used from the three Kingdoms period in Korean history. The styles appeared as the structure of four parts with the lower fences called as nangan, the seats jointed by thin wood pieces, the wind hole, and the legs of foot types. The parts were beautifully carved with the ornament such as lattice patterns like geokjamun and manjamun, flower patterns like dangchomun, elephant eyes patterns like ansangmun from the Korean tradition. And the legs showed various shapes such as supports transformed from horse foots, tiger legs and bamboo, and board types carved with elephant eyes and geometric patterns. Hence, in the next paper based on this paper, PyeongSang will be developed as moulded multi-useful beds and sofas adapting the joints and ornaments from the structure and patterns of the piece.