• International Journal of Industrial Entomology and Biomaterials
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• 제24권2호
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• pp.69-74
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• 2012

Tropical tasar silkworm Antheraea mylitta Drury experiences extreme temperature stress conditions during its life cycle particularly during diapauses and first crop. The present study witnessed the impact of high temperature on some biochemical profiles and egg production (fecundity) of semi-domesticated Daba and Shorea robusta (Sal) based wild ecorace Laria during seed cocoon (pupa) preservation. Cocoons of Daba and Laria were treated with high temperature at $40^{\circ}C$ for 10 days in a BOD incubator. The protein profile and carbohydrate content in the hemolymph and fat body and total haemocyte count (THC) in the hemolymph of pupa were investigated. Further, the fecundity and fertility of egg was assessed. Significant increase in the protein concentration was observed in the hemolymph with reduction in the fat body (p<0.05). The difference in protein concentration was highly significant between the semidomesticated Daba and wild ecorace Laria (p<0.05). High pupal mortality (20%) and reduced fecundity (10-15%) in Daba was noticed compared to wild Laria. Also an increased THC (>28000) was recorded in Laria. The study infers the potentials of wild ecoraces in sustaining the extreme temperature conditions and need of adopting suitable package of practices for the preservation of diapause seed cocoons during extreme summer conditions. There is possibility to introgression thermal stress resistant traits in the semi-domesticated races of tasar silkworm by resorting to conventional breeding plans with wild races and keeping the thermal stress induced response as markers.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.43-43
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• 2018

Mutation breeding by radiation is useful for improving various crop species. Up to now, a total of 170 soybean mutant varieties have been released in the world, which is the second most registered varieties after rice. Despite the economic importance of soybean, there have been no TRAP marker system studies on genetic relationships between/among mutant lines. To develop a strategy of Mutant Diversity Pool (MDP) conservation, a study on the genetic diversity of 210 soybean mutant lines (8 cultivars and 202 mutants) was performed through a TRAP analysis. Sixteen primer combinations amplified a total of 551 fragments. The highest (84.00%) and lowest (32.35%) polymorphism levels were obtained with primers MIR157B + Ga5 and B14G14B + Ga3, respectively. The mean PIC values 0.15 varied among the primer combination ranging from 0.07 in B14G14B + Sal2 to 0.23 in MIR157B + Sa4. Phylogenetic, principal component analysis (PCA) and structure analysis indicated that the 210 lines belong to four groups based on the 16 combination TRAP markers. AMOVA showed 21.0% and 79.0% variations among and within the population, respectively. Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. Our results suggest that the TRAP marker system may be useful for assessing the genetic diversity among soybean mutants and help to improve our knowledge of soybean mutation breeding.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2801-2805
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• 2015

Background: This study was conducted with the aim of determining how students' perceived smoking-cancer relationship and cardiovascular health attitudes affect childrens' views of smoking. Materials and Methods: The sample of this descriptive-cross sectional study comprised 574 subjects between the ages of 11-15. The data were collected using the Children's Cardiovascular Health Promotion Attitude Scale and the Children's Decisional Balance Measure for Assessing and Predicting Smoking Status. Correlation and logistic regression were used for analysis. Results: It was determined that a statistically significant relationship exists between the attitudes of children towards smoking and their ideas about the relationship of smoking with cancer, which is negative and low (r=-0.223). There was also a statistically significant relationship between their attitudes towards cardiovascular health and their attitudes towards smoking, again at a low level (r=0.257). It was determined that children with ideas about smoking and cancer were 9.4 times less likely to have positive/negative attitudes towards smoking, while positive attitudes towards cardiovascular health made negative attitudes towards smoking 3.9 times less likely. Conclusions: It was determined that the attitudes of students towards cardiovascular health and their perceptions of smoking and cancer reduced the positive perceptions towards smoking.

• Journal of The Korean Association For Science Education
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• 제16권2호
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• pp.190-199
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• 1996

The purpose of this study was twofold: (1) to design a system for constructing Likert attitude scales as supported by the sociopsychological and measurement literature, and (2) (using the design) to develop an affective component of "Attitudes toward Science" scale for high school students. The rationale for developing a new attitude scale is presented in the context of a review of existing attitude scales. As discussed in the literature review, many existing attitude scales are based on ill-defined theoretical constructs, and includes statements that do not appear to be assessing a single construct of attitude toward science. In addition, existing attitude scales do not distinguish between affective and behavioral and cognitive components of attitude toward science. Thus, this study was to carefully define the construct, subcomponents of attitude toward science, and develop an affective component of "Attitude toward Science" scale to reflect the construct and to distinguish between affective and behavioral and cognitive components of attitude toward science. The results of this study: (1) three-mains step for designing reliable and valid attitude scale were developed, and (2) 35 items(16 positive and 19 negative) for an affective component of "Attitudes toward Science" scale with the following characteristics were developed: (a) The internal consistency was estimated using Cronbach's coefficient ${\alpha}$, 0.9727. (b) The range of adjusted item-total correletion(${\gamma}$-value) were 0.58${\sim}$0.83. (c) The correlation coefficient ranged between 0.61 and 0.74 revealing a moderate relatedness between subcomponents. (d) The correlation coefficient for concurrent validity were 0.55 with TOSRA and 0.51 with SAl.

• The korean journal of orthodontics
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• 제24권4호
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• pp.773-785
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• 1994

It has been generally believed that the impaction or eruption of mandibular third molars has significant correlations with the growth of the mandible, size of mandibular arch and size of teeth. The purpose of this study was to examine if there is any correlation between the status of eruption of mandibular third molars and the amount of mandibular crowding. The effect of missing of third moalrs to mandibular crowding was studied as well. 140 adult students of Yonsei University were selected and divided into three groups according to the status of mandibular third molars, Group 1) congenital missing group, Group 2) eruption group, and Group 3) impaction group. The tooth size, dimensions of the mandibular dental arch, and the amount of crowding were measured and compared. Results were as follows ; 1. There was no statistically significant difference in tooth size between the missing group and the eruption group(p<0.05). Impaction group showed larger total tooth material, inci- sal tooth material, and individual tooth size except central incisors compared to missing group (p<0.05). Impaction group showed larger total tooth material, incisal tooth material compared to eruption group as well(p<0.05). When individual tooth size was compared, impaction group had larger central incisors, canines, and second premolars than eruption group(p<0.05). 2. Missing group showed larger intermolar width than impaction group and it was stati- stically significant(p<0.05). 3. When compared the amount of total crowding, impaction group showed the largest am- ount of crowding, then eruption group, and missing group showed the least amount of crowding respectively(p<0.05). There was no statistically significant difference between missing stoup and eruption group in the amount of incisal crowding(p<0.05). Impaction group show- ed the largest amount of incisal crowding among all three groups(p<0.05).

• Journal of Environmental Science International
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• 제5권2호
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• pp.179-185
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• 1996

This study was conducted to investigate the effects of environmental factors such as temperature, salinity, pH, and UV light on-the survival of life-threatening Vibrio vulnificus. In the temperature range of 15 to $25^{\circ}C$, the numbers of V. vulnificus incieased during the 6-day incubation, but outside this range, the survival of V. vulnificus was poor. Incubation between 1 and $10^{\circ}C$ showed that V. vulnifcts survived poorly below $10^{\circ}C$. At sal:nities between 5 and 25ppt, the numbers of V. vulnificus increased or remained unchanged for 6-day. At salinities above this range, the numbers of V. vulnificus decreased. The optimal pH range was 6.5 to 8.0 and outside this range, the survival ratio of V. vulnificus was small. At 15-and $25^{\circ}C$, UV radiation was bactericidal for cultures of V, vulnificus. The counts of V. vulnificus were reduced approximately 10, 000-fold after 2h of UV light treatment at both temperatures. Above results mowed 1ha't environmental factors were effective on the survival of V. vulniucus in the environment.

• BMB Reports
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• 제35권6호
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• pp.637-641
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• 2002

Only one natural promoter that interacts with bacteriophage K11 RNA polymerase has so far been identified. To identify more, in the present study restriction fragments of the phage genome were individually assayed for transcription activity in vitro. The K11 genome was digested with two 4-bp-recognizing restriction enzymes, and the fragments cloned in pUC119 were assayed with purified K11 RNA polymerase. Eight K11 promoter-bearing fragments were isolated and sequenced. We report that the nine K11 promoter sequences (including the one previously identified) were highly homologous from -17 to +4, relative to the initiation site at +1. Interestingly, five had -10G and -8A, while the other four had -10A and -8C. The consensus sequences with the natural -10G/-8A and -10A/-8C, and their variants with -10G/-8C and -10A/-8A, showed nearly equal transcription activity, suggesting residues at -10 and -8 do not regulate promoter activity. Using hybridization methods, physical positions of the cloned promoter-bearing sequences were mapped on SalI-and KpnI-restriction maps of the K11 genome. The flanking sequences of six cloned K11 promoters were found to be orthologous with T7 or T3 genomic sequences.

• Food Science and Preservation
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• 제13권2호
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• pp.241-245
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• 2006

The biofunctional properties of traditional Doenjang supplemented with the extract of herb medicines (refer as DHM) were examined DHM divided to four groups, I, II, III and IV according to herb medicines were added. Nitrite scavenging-activities from all 4 groups of DHMs were significantly higher than that of control. Also hydrogen donating-activities from all 4 groups of DHMs were slightly higher than that of control. Antibacterial activities against pathogenic bacteria such as Streptococcus mutant and Salmonella enteritidis and fibrinolytic activity of all DHMs on fibrin plate were higher than those of control. On the other those of groups III and IV were higher in sensory score, biofunctional and antimicrobial activities than those of group I, II and control. Therefore, the usage of extract of Korean herb medicines instead of water to make Doenjang will effective in development of traditional soybean Doenjang with the biofunctional properties.

• The Journal of Korean Society of Virology
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• 제27권2호
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• pp.151-160
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• 1997

In view of the potential of replicase protein as a diagnostic reagent for human caliciviruses (HuCVs), we have cloned and over-expressed this gene from the Snow Mountain-like Korean strains in Escherichia coli as a fusion protein with glutathione S-transferase (GST), and described the preliminary antigenic characterization of the recombinant products. Each 470bp fragment corresponding to highly conserved region of RNA-dependent RNA polymerase was generated by RT-PCR from stools of two diarrheal children, cloned in pMOSBlue T-vector, and subcloned between the EcoRI and SalI restriction sites of pGEX-4T-3, a GST gene fusion vector, yielding $pGCV_{pol}$. This construct expressed a Snow Mountain-like HuCV replicase under the control of the IPTG-inducible tac promoter. An extract prepared by sonication of the E. coli cell inclusion bodies bearing $pGCV_{pol}$ products was purified and analyzed by SDS-PAGE. After Coomassie blue staining, it was shown that the recombinant replicase migrated on the gels with an approximate molecular mass of 46.5 kDa, that was subsequently cleaved into a 26 kDa GST fragment and a 20.5 kDa replicase protein upon digestion with thrombin protease. The replicase was recognized on immunoblotting with the sera from symptomatic children with the HuCV-associated diarrhea but not by asymptomatic sera from adults. The results presented the first biological activity of individually expressed HuCV replicase subunit and provided important reagents for diagnosis of HuCV infection.

• Microbiology and Biotechnology Letters
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• 제24권1호
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• pp.105-110
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• 1996

Mugwort has been known as a traditional substitutive foodstuff and as showing a physiologically beneficial function to a human being. Therefore, effect of mugwort extract in terms of mutagenicity and desmutagenicity was investigated to berify its function. Ethanol extract from mugwort did not exhibit any mutagenicity. On the contrary, inhibitory effects of the ethanol extract were observed on mutagenicity induced by aflatoxin $B_{1}(AFB_1)$, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole(Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2) and 2-nitroflourene(2NF) using Salmonella typhimurium reversion assay. On direct-acting mutagen(2NF, 3${\mu}$g/plate), ethanol extract showed a slight inhibitory effect of 19.7~22.9%, however on indirect-acting mutagen such as AFB1(2${\mu}$g/plate), Trp-P-1(1${\mu}$g/plate) and Trp-P-2(1${\mu}$g/plate), we observed higher inhibitory effect of 47.9~61.2%, 64.1~70.7%, 67.4~78.7%, respectively. Step-wise fractionation of the ethanol extract was done by using hexane, chloroform, ethyl acetate and water to obtain effective fraction. Among them, hexane, chloroform, and ethyl acetate fractions showed high inhibition of 63.0~80.0%, 77.5~82.1%, and 68.5~83.1%, respectively on the mutagenicity of $AFB_1$ in Sal. typhimurium TA98. Consequently, these results indicated that mugwort extract contains some compound(s) which may show desmutagenicity.