• Journal of Food Hygiene and Safety
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• v.31 no.5
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• pp.327-334
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• 2016

This study was conducted to establish the standard method for the contents of biotin in milk formulas. To optimize the method, we compared several conditions for liquid extraction, purification and instrumental measurement using spiked samples and certified reference material (NIST SRM 1849a) as test materials. LC-MS/MS method for biotin was established using $C_{18}$ column and binary gradient 0.1% formic acid/acetonitrile, 0.1% formic acid/water mobile phase is applied for biotin. Product-ion traces at m/z 245.1 ${\rightarrow}$ 227.1, 166.1 are used for quantitative analysis of biotin. The linearity was over $R^2=0.999$ in range of $5{\sim}60{\mu}g/L$. For purification, chloroform was used as a solvent for eliminating lipids in milk formula. The linearity was over 0.999 in range of 5~60 ng/mL. The detection limit and quantification limit were 0.10, 0.31 ng/mL. The accuracy and precision of LC-MS/MS method using CRM were 103%, 2.5% respectively. Optimized methods were applied in sample analysis to verify the reliability. All the tested milk formulas were acceptable contents of biotin compared with component specification and standards for nutrition labeling. The standard operating procedures were prepared for biotin to provide experimental information and to strengthen the management of nutrient in milk formula.

• Korean Journal of Environmental Agriculture
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• v.40 no.2
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• pp.108-117
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• 2021

BACKGROUND: Dichlobentiazox is a newly registered pesticide in Korea as a triazole fungicide and requires establishment of an official analysis method for the safety management. Therefore, the aim of this study was to determine the residual analysis method of dichlobentiazox for the five representative agricultural products. METHODS AND RESULTS: Three QuEChERS methods were applied to establish the extraction method, and the EN method was finally selected through the recovery test. In addition, various adsorbent agents were applied to establish the clean-up method. As a result, it was found that the recovery of the tested pesticide was reduced when using the d-SPE method with PSA and GCB, but C₁₈ showed an excellent recovery. Therefore this method was established as the final analysis method. For the analysis, LC-MS/MS was used with consideration of the selectivity and sensitivity of the target pesticide and was operated in MRM mode. The results of the recovery test using the established analysis method and inter laboratory validation showed a valid range of 70-120%, with standard deviation and coefficient of variation of less than 3.0% and 11.6%, respectively. CONCLUSION: Dichlobentiazox could be analyzed with a modified QuEChERS method, and the method determined would be widely available to ensure the safety of residual pesticides in Korea.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.3
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• pp.85-91
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• 2021

Type 1 and type 2 buildings must regularly conduct precise safety inspections and precise safety diagnosis for the safety and maintenance of facilities, and the safety grade of the building is determined according to the results of the implementation. In addition, the cycle of inspection and diagnosis is determined according to the safety grade of the building. In order to determine the safety grade of the building, a precise safety inspection conducts condition evaluation, and a precise safety diagnosis conducts condition evaluation and safety evaluation. Therefore, since the inspection and diagnosis cycle is determined according to the safety grade of the building, the condition evaluation and safety evaluation must be precise. However, in the case of member unit evaluation, which is the first step in evaluating the current condition, the evaluation grade is determined by using the representative value of the measurement result, and this may result in an error in the evaluation grade. To solve this problem, this study analyzed evaluation criteria for each evaluation item and presented evaluation criteria using inequalities to respond to measurement results and evaluation scores. In addition, we present a functional formula that can reflect performance scores for each evaluation item.

• Proceedings of KOSOMES biannual meeting
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• 2018.06a
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• pp.42-42
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• 2018

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.97-97
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• 2019

사삼(沙蔘, Adenophorae Radix)은 "대한민국약전외한약(생약)규격집(KHP)"에 잔대 Adenophora triphylla var. japonica Hara 또는 사삼(당잔대, A. stricta Miq.)의 뿌리로 수재되어 있으나, 형태학적으로 유사한 제니(모시대, A. remotiflorus Miquel), 층층잔대(윤엽사삼, A. tetraphylla (Thunb.) Fisch), 더덕 Codonopsis lanceolata (Sieb. et Zucc.)과 오 혼용 우려가 있어 이들을 구별하기 위한 종 감별법이 필요하다. 본 연구에서는 '사삼'과 오 혼용 우려가 있는 종들을 구별할 수 있는 유전자 마커 개발을 위하여 DNA 바코드로 활용되고 있는 유전자 부위를 분석하여 ITS (25%), atpB-rbcL (15%), atpF-atpH (14%), rpl16 (13%), trnL-F (10%), matK (9%), rpoC1 (7%)에서 변이율(percent of variable sites)을 확인하였다. 또한, 분석한 유전자 부위 중 종간 차이를 확인하기 용이한 matK 구간을 활용해 기원종인 잔대, 당잔대와 형태적으로 유사하여 오 혼용될 우려가 있는 층층잔대, 모시대 및 더덕을 감별 할 수 있는 유전자 마커를 개발하였다. 본 연구를 통해 얻어진 염기서열과 분자 마커는 '사삼'의 품질관리에 유용하게 활용 가능할 것으로 사료된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.98-98
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• 2019

제니(薺苨, Adenophorae Remotiflori Radix)는 "대한민국약전외한약(생약)규격집(KHP)"에 모시대(Adenophora remotiflorus Miquel)의 뿌리로 수재되어있으나, 형태학적으로 유사한 잔대(A. triphylla), 당잔대(A. stricta) 및 더덕(Codonopsis lanceolata)과 오 혼용 우려가 있어 이들을 구별하기 위한 정확하고 객관적인 종 감별법이 필요하다. 본 연구에서는 '제니'의 기원인 모시대와 오 혼용 우려가 있는 종들을 구별 할 수 있는 유전자 마커를 개발하기 위하여 Genbank에 등록된 ycf2 구간을 활요하여 모시대와 잔대, 당잔대를 구분 할 수 있는 INDEL (insertion/deletion) 마커를 개발하였다. 또한, 보다 정확한 종감별을 위해 DNA 바코드로 활용되고 있는 유전자 부위의 염기서열을 분석하여 ITS (25%), atpB-rbcL (15%), atpF-atpH (14%), rpl16 (13%), trnL-F (10%), matK (9%), rpoC1 (7%)에서 변이율(percent of variable sites)을 확인하였다. 향후, 본 연구에서 개발된 INDEL 마커와 더불어 추가적으로 개발을 진행 중인 분자 마커는 한약재 '제니'의 품질관리에 활용 가능할 것으로 사료된다.

• Journal of the Ergonomics Society of Korea
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• v.35 no.2
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• pp.75-84
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• 2016

Objective: The aim of this study is to suggest some improvement ideas based on the validity and the reliability analyses of the current safety culture measurement method applied to the Korean nuclear power industry. Background: Wrong safety culture is known as one of the major causes of the disasters such as the space shuttle Columbia disaster or the Fukushima Nuclear Power Plant accident. Assessment of safety culture of an organization is important to build a safer organizational environment as well as to identify the risks hidden in the organization. Method: A face validity of the current safety culture measurement method was analyzed by comparison of the key factors of safety culture in the Korean nuclear power industry with those factors reviewed in the previous studies. The current interview method was analyzed to identify the problems which degrade the consistency of evaluation. Results: Most safety culture factors reviewed in the literatures are covered in the list of the Korean nuclear power industry safety culture factors. However the unstructured questions used in the interview may result in inconsistency of safety culture evaluation among interviewers. Conclusion: This study suggests some examples which might improve the consistency of interviewers' evaluation on safety culture such as a post interview evaluation form. Application: An extended post interview evaluation form might help to increase the accuracy of the interviewing method for Korean nuclear industry safety culture evaluation.

• Journal of Korean Society for Quality Management
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• v.49 no.2
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• pp.127-143
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• 2021

Purpose: Smart Safety Living Lab is a Living Lab facility, constructed and operated by KITECH in Korea, to support the user experience(UX) evaluation, planning and certification of smart safety products and services. The purpose of this study is to develop a UX evaluation methodology that accommodates the characteristics of the Living Lab and smart safety products and services for a systematic and efficient UX evaluation in the Smart Safety Living Lab. Methods: A generic model of UX evaluation was first derived based on a review of related literature. Then, the generic model is revised to accommodate the characteristics of the Smart Safety Living Lab and smart safety products and services, resulting in the UX Evaluation Methodology for Smart Safety Living Lab (SSLL-UXEM). Results: The developed SSLL-UXEM consists of a structured process for UX evaluation, a guideline for conducting each step of the process, and a set of forms for recording the major evaluation results in each step. Conclusion: SSLL-UXEM can help to enhance the efficiency of the UX evaluation process and the consistency of the UX evaluation results. SSLL-UXEM is also expected to serve as a basis for UX evaluation in various living lab environments in the future.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1657-1667
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• 2007

This study propose a model for railway safety evaluation with which the safety of whole railway system can be evaluated. The evaluation model is to generate a safety index which quantitatively represent the degree of railway safety. Safety index is proposed a function of three indexes; an accident index, safety management index, and safety culture index. This paper describes the first result from the study on the safety target which will be a key starting point toward the development of safety evaluation model. It is recommended that the safety target be composed of several sub-targets that are apportioned to constituent components. It is concluded that the classification of safety target influence on deciding components or attributes that constitute each sub-indexes; an accident index, safety management index, and safety culture index. Based on this study, a railway safety evaluation model will be developed in the next study.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.363-369
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• 2014

Synthetic cannabinoids (CBs) such as the JWH series have caused social problems concerning their abuse liability. Because the JWH series produces euphoric and hallucinogenic effects, they have been distributed illegally under street names such as "Spice" and "Smoke". Many countries including Korea have started to schedule some of the JWH series compounds as controlled substances, but there are a number of JWH series chemicals that remain uncontrolled by law. In this study, three synthetic CBs with different binding affinities to the $CB_1$ receptor (JWH-073, 081, and 210) and ${\Delta}^9$-tetrahydrocannabinol (${\Delta}^9$-THC) were evaluated for their potential for psychological dependence. The conditioned place preference test (unbiased method) and self-administration test (fixed ratio of 1) using rodents were conducted. $K_i$ values of the three synthetic cannabinoids were calculated as supplementary data using a receptor binding assay and overexpressed $CB_1$ protein membranes to compare dependence potential with $CB_1$ receptor binding affinity. All mice administered JWH-073, 081, or 210 showed significantly increased time spent at unpreferred space in a dose-dependence manner in the conditioned place preference test. In contrast, all tested substances except ${\Delta}^9$-THC showed aversion phenomenon at high doses in the conditioned place preference test. The order of affinity to the $CB_1$ receptor in the receptor binding assay was JWH-210 > JWH-081 >> JWH-073, which was in agreement with the results from the conditioned place preference test. However, no change in self-administration was observed. These findings suggest the possibility to predict dependence potential of synthetic CBs through a receptor binding assay at the screening level.