• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.1
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• pp.32-40
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• 2017

The aim of this study was to evaluate a temperature-induced two-stage cultivation (TTC) strategy for the regulation of lipid and carbohydrate production by two microalgae, Chlorella vulgaris and Dunaliella salina, for biofuel production. The microalgae were grown under several temperature conditions (15, 25, 35, and $45^{\circ}C$) and optimal growth was observed at $25^{\circ}C$ for both microalgae. To test the TTC, aseptically cultured microalgae were incubated under optimal conditions ($25^{\circ}C$) for 20 days, and then divided into four aliquots that were incubated at 15, 25, 35, and $45^{\circ}C$ for 5 days. Similar but somewhat decreased growth rates were observed at the non-optimal temperatures (15, 35, and $45^{\circ}C$). In addition, while total lipid accumulation increased in a temperature-dependent manner in both microalgae, total carbohydrate increased with temperature in C. vulgaris but decreased in D. salina. However, for lipid and carbohydrate production, while the highest lipid productions of C. vulgaris and D. salina were observed at $25^{\circ}C$ and $35^{\circ}C$, respectively, the highest total carbohydrate productions of C. vulgaris and D. salina were obtained at $15^{\circ}C$ and $25^{\circ}C$, respectively. These results suggest that the TTC strategy may be easily and efficiently applied to bioprocessing for biofuel production.

• Korean journal of food and cookery science
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• v.28 no.6
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• pp.865-870
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• 2012

This study was performed to investigate the effect of steamed garlic powder (SGP) on the quality characteristics of Jochung, saccharide by barley melt. Physicochemical properties and sensory quality were determined in Jochungs were prepared that 5% (SGP 5), 10% (SGP 10), 20% (SGP 20) and 30% (SGP 30) of SGP to sikhe for making jochung and hard boiled (W/W). Turbidity and viscosity of SGP added groups increased than control, significantly. The viscosity was $133{\pm}10^3$ cps in control group, it higher than SGP added groups ($140{\pm}11^3{\sim}272{\pm}2^3$ cps). The pH decreased with increasing SGP concentration. Reducing sugar was significantly higher in SGP added groups than control group, especially 30% SGP addition group in $44.66{\pm}0.26$ mg/100 g. HMF (Hydroxymethyl-furfural) content increased with increasing of SGP concentration, and 30% SGP addition group was higher in 48% than control group. In HPLC determination, free sugars content increased with amounts of SGP addition level. Fructose and glucose contents increased while maltose and raffinose contents decreased. Fructose content was $0.84{\pm}0.02$ mg/100g in control group but $1.36{\pm}0.03{\sim}1.62{\pm}0.01$ mg/100 g in SGP added groups. In the sensory evaluation, the color of Jochung decreased dose dependently with the SGP. Garlic flavor was significantly greater in SGP added groups compared to that of control group. The overall acceptability had not significantly difference among control, SGP 5 and 10 group. Preference was decreased in contain more than 20% of SGP added groups. The optimal concentration of SGP was found in the range of less than 10%.

• Tuberculosis and Respiratory Diseases
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• v.61 no.3
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• pp.256-264
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• 2006

Background: An acute lung injury(ALI) is characterized by the recruitment, activation, and apoptosis of inflammatory cells, numerous products released by inflammatory cells such as reactive oxygen species, inflammatory mediators, and a variety of proteolytic enzymes. It was reported that bacterial infections in diabetics showed impaired PMN functions such as reduced PMN respiratory burst and decreased microbicidal activity in inflamed tissue. However, the effect of the proteinase - inhibitor (MMP-9 vs TIMP-1) in ALI in diabetics is unclear. This study evaluated the differences in the expression of MMP-9 and TIMP-1 after the stimulation of endotoxin in a rat model. Methods: Six-week-old male Sprague-Dawley rats were classified into normal, DM, LPS and DM+LPS groups. The peripheral blood, BAL fluids, and lung tissues were obtained from individual rats. The MMP-9 activity was measured by gelatin zymography and the TIMP-1 level was measured by Western blotting. Results: The total BAL cells of the DM-LPS groups were significantly lower than the LPS groups (p < 0.01). The MMP-9 activities in the serum were higher in the DM+LPS groups than in the other groups. The MMP-9 activities in the BAL fluids were significantly higher in the DM+LPS group than in the normal and diabetic rats (p < 0.05). TIMP-1 expressions in the BAL fluids were significantly lower in the DM+LPS group than other groups (p < 0.05). The ratio between MMP-9 and TIMP-1 in the BAL fluids was significantly higher in the DM+LPS groups (p < 0.05). Conclusion: In ALI in diabetics the higher MMP-9 activity and lower TIMP-1 level are believed to prolonged and intensify the course of inflammation.