• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.289-298
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• 1992

Acanthamoeba sap., free-living amoebae inhabited in moist soil, pond, freshwater, sewage, atmosphere and swimming pool, may be causative protozoa of the fatal primary amoebic meningoence-phalitis in experimental animals and humans. In this study, Acar,thamoeba spry. , including Acan. thamoeba sp. YM-4 (isolated strain from Korea) had been compared by the two-dimensional electrophoresis and hybridoma technique as well as the difference of morphological characteristics. Trophozoite of Acenthamoeba sp. YM-4 is usually uninucleate and show the hyaline filamentous projections (acanthopoda) . No aagellate stage observed. Cysts have two walls, the outer wall is nearly circular, but inner wall is oval or some irregular. As results of SDS-PAGE for Iysate of Acanthamoeba sp. VM-4, 16 major protein fractions are similiar to those of A. cuzbertsoni, but different to A. royreba and A. polyphaga. Findings of two-dimensional electrophoretic patterns of Acanthamceba sp. YM-4 are almost same to those of A. culberssoni, The isotope of monoclonal antibodies produced from McAY 6, McAY 7, McAY 8, McAY 13 and McAY 16 clones were IgGl, and McAY 10 and McAY 11 clones were IsM. As results of the cross-reactivity among various amoebae using ELISA with monoclonal antibodies, McAY 7 monoclonal antibody (molecular weight 43 kDa by EITB) was only reacted with Acanthamoeba sp. YM-4, but McAY 6 and McAY 10 monoclonal antibodies were reacted to A. cuzbertsoni as well as Acanthamoeba sp. YM-4.

• Journal of fish pathology
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• v.6 no.2
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• pp.133-142
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• 1993

Vitamin B6(pyridoxine, pyridoxamine. and pyridoxal) is a dietary requirement in relatively small quantities for growth, health, and function in animals and fish. The metabolically active B6 is pyridoxal-5-phosphate(PLP). It does function as a coenzyme in number of enzymes(PLP-dependent enzymes) in which amino acids are metabolized, including decarboxylases, aminotransferases, sulfhydrases, tryptophanase, and hydroxylases. Vitamin B6 requirement is higher for fish because fish are fed much higher protein diet than land animals. B6 is also involved in metabolism of carbohydrates and lipids and essential for the synthesis of heme and serotonin. Deficiency signs in fish develop quickly, in cluding nervous disorders, convulsions, poor swimming coordination, skin lesions, edema, exophthalmos, and tetany. The conversion of vitamin B6 to metabolically active form(PLP) is catalyzed by pyridoxal kinase and pridoxine(pyridoxamine) oxidase. In this review, we summarized in detail the enzymatic studies on vitamin B6 metabolism and about the mechanisms and properties of a PLP-dependent enzyme.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.1
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• pp.78-84
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• 2010

This study was to designed to investigate the effects of nutrition education and exercise program for overweight or obese female adults, and also emphasize the necessity of a proper nutrition education. The anthropometric characteristics, nutritional intake, eating habit, and hematological index, physical examination were measured before and after program. Considering the time schedule of the subjects, the exercise program was divided into 3 sessions (morning, afternoon, and evening) and the subjects performed swimming and weight training for 4 weeks. The subjects were overweight or obese women, with an average age and height of each 46.1 and 156.3 cm. The weight and BMI were decreased significantly after nutrition education and exercise program. The back region of front arm (p<0.001), infrascapular region (p<0.001), body fat (p<0.001) were significantly decreased, and grasping power (p<0.01), back muscle power (p<0.001) were significantly increased after nutritional and exercise program. Total triglyceride level and fasting blood glucose (p<0.001) were decreased significantly after program. The intakes of vitamin D and vitamin $B_1$ were significantly changed after nutrition and exercise program. As for eating behavior and nutritional knowledge evaluation after program, the average score increased significantly (p<0.001). The results of this study show that this nutrition and exercise program can be an effective approach to improve their hematological index and nutritional and health status.

• The Journal of Pediatrics of Korean Medicine
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• v.12 no.1
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• pp.111-131
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• 1998

1. In oriental medicine, the case of sinusitis can be explained, not only external invasion such as PUNG HAN(wind-cold), PUNG YUL(wind-hot), SHUP YUL(damp-heat), but also functional disorder of internal organ such as spleen(脾), lung(肺), kidney(腎) The western medicine classified the cause of sinusitis as two factors. That is bacterial infection factor-Haemophilus influenza, streptococcus pyogeues, streptococcus pneumonia- and viral infection factor-Rhinovirus, parainfluenza, Echo28, Coxsacki21, Sinusitis is complicated to allergic rhinitis, chronic otitis media purulent, chronic tonsilitis, sinubronchitis. On the condition of nasal septum deformity, turbinates deformity, nasal septum deviation, sinusitis can be developed. the predisposing factors of sinusitis is swimming, air pollution, malnutrition, shortage of immunity.2. According to survey, sinusitis occurred that children from 4 to 12 years old and from 5 to 7 years old occupied 70% 3. From the past history data, they experienced chronic tonsillar hypertropy(20%), otitis media, atopic dermatitis, allergic rhinitis, bronchial asthma, pneumonia, bronchiolitis, chronic sore throat, urticaria, milk allergy in sequence. 4. the symptoms of sinusitis is nasal obstruction, postnasal dripping, purulunt(yellow)or white discharge, cough, nose bleeding in sequence. nasal obstruction take the portion of 95%, postnasal dripping 65%, night time or early morning cough 60%. 5. The suffering period of sinusitis is 6 month minimum, 4 years maximum, most cases are included in a year. The suffering period of children was shorter than adult. 6. Diagnosis depend on inspection of nasal cavity, postnasal dripping, X-ray finding. 75% of patient(15case) showed both maxillary sinusitis, 25%(5cases) showed left or right maxillary sinusitis. 7. Treatment of oriental medicine, consist of Herb-medicine, acupuncture and exposing of Lazer beam. Kamihyunggyeyungyotang(加味荊芥蓮翹湯) is administered mainly as the medical therapy, Kamigwaghyangjeungkisan(加味藿香正氣散) Kimizwakwieum(加味左歸飮), Kamihyangsosan(加味香蘇散) is administered for a additional symptoms which occurred by influenza recurrence. Kamijeonxibackchulsan(加味錢氏白朮散) is administered to treat gastro-intestine trouble patients who have sinusitis. 8. The period of treatment is varied with patient conditions and X-ray finding. The minimal period is 35days, maximal period is 202days. So it took about 86days in average and about 50% of patient(10cases) is recovered in one or two month.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.528-533
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• 2004

The immune system acts to protect the host from infectious agents that exist in the environment and from other noxious insults. The immune system has two functional divisions: the innate and the acquired. Both components involve various factors such as cytokines. A number of methodologies exist to assess aspects of immune function. There are large inter-individual variations in many immune functions even among the healthy. Genetics, age, gender, smoking habits, habitual levels of exercise, alcohol consumption, diet, stage in the female menstrual cycle, stress, history of infections and vaccinations, and early life experiences are likely to be important contributors to the observed variation. While it is clear that individuals with immune responses significantly below 'normal' are more susceptible to infectious agents and exhibit increased infectious morbidity and mortality, it is not clear how the variation in immune function among healthy individuals relates to variation in susceptibility to infection. Oriental medicine is an important factor contributing to immune competence. The author investigated the immune enhancement effects of Bojungikkitanggami-bang (BITB). The forced swimming test (FST) has been used as a screening model for new immune enhancement agents. In the present study, the author investigated the effects of BITB on FST and blood biochemical parameters related to fatigue, glucose (Glc); blood urea nitrogen (BUN); lactate dehydrogenase (LDH); creatinine; and total protein (TP). The author found that BITB (1 g/kg) significantly reduced the immobility time in the FST compared to the control. In addition, the contents of Glc, LDH, BUN, TP in the blood serum were increased in BITB (1g/kg)-fed group. Also, the author investigated the effects of BITB on the production of cytokines in human T-cell line, MOLT-4 cells. BITB (1 mg/ml) significantly increased the interferon (IFN)-vproduction compared with media control (about 2.2-fold for IFN-γ) at 24 h. However, BITB has not affect the production of IL-2 and IL-4. In addition, BITB increased the protein expression level of IFN-γ in MOLT-4 cells. Thus, BITB may have therapeutic value in generating or enhancing immune function in a clinical setting.

• The Journal of the Korea Contents Association
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• v.17 no.7
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• pp.283-293
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• 2017

This study analyzed the actual conditions of use of public sports facilities and characteristics of the users of the facilities through surveys and measured the spatial imbalance of the public sports facilities currently supplied by using gravity potential model. This study also suggests evaluation criteria that may be considered for efficient location selection by examining the change of accessibility to the facilities that meet the needs of users in the future. As the results of the questionnaire survey, unlike current usage, the users hoped for badminton, weight training and swimming. And we could confirm the demand for the expansion of the multi - purpose indoor gym which can carry out such activities in the areas. As the result of the analysis on the difference in accessibility of the public sports facility, there were some large variations in the regions. It was found that a balanced supply of facilities was needed in terms of equity. In particular, when analyzing by considering the population estimates of 2025, It is analyzed that the accessibility will be reduced to about 60% compared to that of 2015. In addition, it is evaluated as the best alternative in terms of overall efficiency that the location of the facilities should be in Munsan area where population growth is expected in the future.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.38 no.3
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• pp.241-248
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• 2002

The combined hydroacoustic and demersal trawl surveys to investigate the distribution characteristics of fish schools in the Yellow Sea and the East China Sea were carried out during the spring of 1997 by the training ship "Nagasaki Maru" of Nagasaki University. Fish samples were collected by bottom trawling from 9 trawl stations randomly selected in the survey area, and the species and length compositions of trawl catches are examined. Hydroacoustic data were collected by using a Furuno FQ-50 scientific echo sounder operating at 50KHz and the data stored in field were later processed in the laboratory. The results obtained can be summarized as follows ：1. In the 9 trawl surveys conducted in the Yellow Sea and the East China Sea, 78 species including 80 species of fishes, 4 species of Cephalopoda and 6 species of Crustacea, were identified. The most abundant species in these stations were swimming crab(Portunus trituberculatus), Japanese horse mackere(Trachurus japonicus), redlip croaker(Larimichthys polyactis) and the catch per one hour in each station ranged 7.7 to 182.5 kg/hour. 2. The mean volume backscattering strength for the entire water column and the bottom layer of the 0-10 m from bottom friction were -74.6 ㏈ and -68.2 ㏈, respectively. That is, the mean volume backscattering strength for the bottom layer was 6.4 ㏈ higher than that for the entire water column 3. In the surveys during the spring of 1997, the geographical distribution characteristics of fish schools suggests a trend of decreasing fish abundance toward the coast area of Korea and the highest demersal concentrations appeared in waters between the Cheju Island and the Tsushima Island 4. The distribution density of fish school in the East China Sea and Yellow Sea during the 1997 acoustic survey were estimated to be 6.65$\times$10$^{-5}$ kg/㎥ in the entire water column and 2.86$\times$l0$^{-4}$ kg/㎥ in the bottom layer, respectively.pectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.1
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• pp.63-73
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• 1995

A remote telesounder system was manufactured to investigate the behavior and distribution characteristics of fish and was tested to verify its performance and effects int he set net fishing ground located off the Namhae island, Oct. 26~27, 1994. The activity pattern of fish was observed at the buoy station installed in a set net and it was also analyzed simultaneously at the remote base station on land located at distance of about 1.5km away from the buoy station. Several findings at both the buoy station and the base station were as follows: The images of color display monitor at the base station on land were very well coincident with the records observed at the buoy station. The lower part of slope net and bagnet fluctuated irregularly, and its variations were about 3.6m and 6.0m, respectively. In the daytime, the fish school entered playground, passed slope net with 5~7m depth, moved dispersedly at night and at twilight the entrance was rare. In the bagnet, in the daytime the fish school swam in the layer of 8m in depth, at night the fishes dispersed in the entire layer and at twilight they formed dense school. The fishes caught by the set net were about 500kg with 20 species. Anchovy was the most dominant species. In conclusion, the remote telesounder was a convenient and powerful system for investigating the distribution pattern and the swimming behavior of fish entering in set net.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.4
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• pp.75-82
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• 1986

A field experiment was carried out to clarify the effect of underwater sound on the luring of fish school. The effects of. the acoustic emission on the luring of fish school were checked actually at a set net fishing site in Namhae using a commercial acoustic equipment, Dairyo-8. An emitting system of sound was designed by the authors, and the ambient noise, the sound pressure level and the reaction of fish school were measured in the set net. 1. The predominent frequency band of ambient noise was 150Hz-400Hz,.and the sound emitted was 400Hz-100Hz. The sound pressure level of ambient noise in set net was higher at the landing part, and lower at the playgrond, the gate of court and "the enterance of inclined "passage. The ambient noise was increased with the time elapse-d at the stage of hauling net, but :it was decreased suddenly at the final stage due probably to the decrease of the swimming speed of the fish school. 2. The results of the observation and the recording paper of echo sounder indicate that the effect of emitting sound in the bag net of set net was remarkable for the luring of fish school in the early stage, but decreased after 30 minutes. The reaction of fish school is more sensitiv2 to the sound pressure level than the time intervals between the emission and the pause. For the purpos~ of practical use, it is nesessary to confirm what kind of sound pressure level is the best for the luring of fish school. 3. In response to the acoustic equipment(Dairyo-8), fish school started to swarm 20 minutes after the sound emission and scattered when the sound paused. As the emitting pattern of the acoustic equiment, the three seconds of emission after one second of pause was more effective than the continuous emission at the set net fishing ground. Catch of the fish(s during th~ sound ernissio:l at the gate of court was three to five times more than that of no emission.

• Herbal Formula Science
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• v.15 no.2
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• pp.35-45
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• 2007

Objective : The purpose of this report was to provide the information activity and safety of Palmul-tang by analyzing domestic/international papers and theses about Palmul-tang, Methods: Domestic/international papers and theses related to Palmul-tang were reviewed and analyzed, These papers were then classified by year, experimental method and subject, Results: The following results were obtained in this study. 1. The study of Palmul-tang started from 1985 and was continuously increased. The study was mainly forcused on experimental model rather than clinical study. 2. As these studies were classified by subject, papers related to immune intensification were most abundant by 20 papers, Besides there were several papers related to cardiovascular activity, reproductive activity, anti-apoptotic activity and cerebral hemodynamics. 3. Among the papers related to immune intensification. the studies on recovery of fatigue were mostabundant by 10 papers and the studies of on immune cell and cytokine express were six. In addition to. several studies were associated with anti-cancer activity and anti-allergic activity. Recovery of fatigue was determined by measurement of fatigue markers in a living body such as lactate. CPK, pyruvate and triglyceride and assessment of exercise capability of animals such as swimming test. slopped plate test. Rota-rod test, and activity cage test after Palmul-tang treatment. 4. According to experimental data. it is supported that Palmul-tang has been used as Qi and Blood intensifier with immune intensification and recovery of fatigue. 5. The paper related to safety of Palmul-tang was only one paper which is studied on acute toxicity of Palmul-tang with experiment with ICR mouse. There was no study on evaluating safety by observing liver and kidney functions after Palmul-tang treatment Conclusion: Palmul-tang is being used in various ways associating with immune intensification. cardiovascular activity and reproductive activity. However. studies on efficacy and mechanism of Palmul-tang should be conducted at the molecular biology level and studies on safety of Palmul-tang need to be completed at the clinical level.