• KSCE Journal of Civil and Environmental Engineering Research
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• v.44 no.4
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• pp.465-482
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• 2024

The global use of groundwater in coastal areas has increased. Events such as seawater intrusion (SWI) are expected to increase along with the acceleration of natural disasters owing to environmental changes such as climate change, resulting in large-scale damage worldwide. Current trends in the research of coastal groundwater and related natural disasters include testing and verifying technologies using major case studies from individual countries. We identified global research trends in coastal groundwater, related these trends to changing environments and climate, and confirmed the qualitative and quantitative growth of these studies. This study describes the theoretical background and techniques for coastal groundwater analysis and details regional-scale SWI indicators based on analytical and numerical studies. This review highlights recent technologies that consider uncertainty and promotes discussions on field data obtained using new technologies. Finally, the research findings and trends for a regional coastal aquifer in Korea are discussed to describe recent SWI approaches for groundwater resources.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1615-1619
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• 2015

Background: Promoter hypermethylation mediated gene silencing of tumor suppressor genes is considered as most frequent mechanism than genetic aberrations such as mutations in the development of cancers. BRD7 is a single bromodomain containing protein that functions as a subunit of SWI/SNF chromatin-remodeling complex to regulate transcription. It also interacts with the well know tumor suppressor protein p53 to trans-activate genes involved in cell cycle arrest. Loss of expression of BRD7 has been observed in breast cancers and nasopharyngeal carcinomas due to promoter hypermethylation. However, the genetic status of BRD7 in oral squamous cell carcinomas (OSCCs) is not known, although OSCC is one of the most common among all reported cancers in the Indian population. Hence, in the present study we investigated OSCC samples to determine the occurrence of hypermethylation in the promoter region of BRD7 and understand its prevalence. Materials and Methods: Genomic DNA extracted from biopsy tissues of twenty three oral squamous cell carcinomas were digested with methylation sensitive HpaII type2 restriction enzyme that recognizes and cuts unmethylated CCGG motifs. The digested DNA samples were amplified with primers flanking the CCGG motifs in promoter region of BRD7 gene. The PCR amplified products were analyzed by agarose gel electrophoresis along with undigested amplification control. Results: Methylation sensitive enzyme technique identified methylation of BRD7 promoter region seventeen out of twenty three (74%) well differentiated oral squamous cell carcinoma samples. Conclusions: The identification of BRD7 promoter hypermethylation in 74% of well differentiated oral squamous cell carcinomas indicates that the methylation dependent silencing of BRD7 gene is a frequent event in carcinogenesis. To the best of our knowledge, the present study is the first to report the occurrence of BRD7and its high prevalence in oral squamous cell carcinomas.