• Journal of Animal Science and Technology
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• v.53 no.1
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• pp.35-42
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• 2011

The study was conducted to select and optimize microsatellite (MS) markers for evaluation of Korean native pig (KNP) populations in order to provide standard for the classification and breed definition of the indigenous breeds. The study also aimed to characterize and classify each KNP populations. A total of 648 pigs from 17 pig populations including six KNP, four Chinese native pig and four commercial pig populations were analyzed with 26 MS markers. KNP populations formed separate cluster from those of Chinese native pig and introduced pig populations. Expected heterozygosity (He) of KNP populations were 0.48~0.55 except two populations with 0.65. Genetic distances between KNP populations were relatively shorter: 0.12-0.34. Among six KNP populations, three showed high genetic uniformity, two showed lower uniformity and one showed high level of impurity and heterozygosity. The results can be used to evaluate and manage animal genetic resources at national scale.

• Proceedings of the Korea Water Resources Association Conference
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• 1998.05a
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• pp.395-403
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• 1998

오염원의 이송확산에 관한 많은 연구들이 수행되어 왔으나 특히 비포화 영역에서 오염원 이송확산을 측정하는 것은 매우 어려운 것으로 알려지고 있다. 비포화 토양에서의 오염원 이송확산은 매질의 함수량 변화에 영향을 받기 때문에 오염원 거동특성을 이해하려면 비포화 흐름 분석을 선행한 후 오염원의 이송확산 특성을 분석하여야 한다. 본 연구에서는 비포화 영역에서의 오염원 이송특성을 분석하기 위하여 TDR(Time Domain Reflectometry)을 이용하여 비포화 흐름 및 오염원 이송을 측정하였다. 이를 위하여 본 연구에서는 TDR을 이용하여 오염원 이송을 측정하는 방법을 개발하였으며, 이 방법을 이용하여 1차원의 토양기둥시료에서 비포화 흐름 및 오염원 이송확산에 관한 실험을 수행하고 수치모형을 적용함으로써 비포화 영역에서 오염원의 이송확산에 관한 거동특성을 규명하였다. 본 연구에서는 두 종류의 국내 토양시료(SUS, KUS)를 사용하였는데, 토양의 물리적 특성을 예비실험을 통하여 규명한 후 토양기둥시료를 이용한 본실험을 수행하였다. 비포화 천이흐름하의 오염원 이송확산 실험에서는 급격한 습윤전선의 전진에 따른 종형의 함수량변화를 관측할 수 있었고, 이때 오염원의 농도는 함수량의 천이구간의 중심점으로부터 전방영역의 농도분포가 습윤전선에서의 함수량 분포와 유사한 종형을 이루고 있음을 관측할 수 있었다. 비포화 정상흐름하의 오염원 이송확산 실험에서는 오염원이 이송하며 농도 천이구간이 확장되어지는 전형적인 형태를 보였다. 또한 예비실험에서 측정한 매개변수를 입력자료로하여 수행한 수치결과와 실험결과를 비교하였는데 비포화 흐름특성은 실험결과와 수치결과가 정량적으로 일치하는 경향을 보였으나, 오염원 이송확산 특성은 정량적으로 수치결과가 실험결과보다 더 많이 확산되는 경향을 보였다. 따라서 수치모형을 현장에 적용할 경우 확산지수 결정에 주의하여야 할 것으로 판단된다. 즉, 수치모형에 적용할 확산지수는 BTC 실험을 통하여 측정한 확산지수, 수치확산, 흡착계수, 적용영역의 크기 등을 고려하여 결정하여야 한다. 특히 본 논문에서는 TDR을 이용하여 최초로 천이상태의 함수량과 오염원 농도를 측정하였는데 이를 위하여 전기전도도와 함수량관계를 추정하는 식을 제안하였으며, 전기전도도와 토양수 농도, 전기전도도와 함수량의 관계를 이용한 천이상태의 오염원 농도 측정방법을 개발하였다. 특히 제안식에서는 한계함수량의 개념을 도입하여 전기전도도와 함수량관계를 추정하므로 추정식의 실험값 반영 정도를 증가시켰다. 본 연구에서 제안된 식을 이용하여 추정된 전기전도도와 함수량관계는 다른 제안식에 비하여 개선된 결과를 보여 주었고, 본 연구에서 개발한 오염원 농도 측정법을 이용하여 측정한 결과 함수량이 0.15이하에서는 측정오차가 크지만 함수량이 0.15이상일 경우 매우 좋은 결과를 보였는데 질량평형을 검토한 결과 약 5-10%의 오차율을 보였다. 따라서 본 논문에서 개발된 천이상태의 오염원 농도측정법은 용존 오염물질의 이송에 관한 정확한 실험을 제공할 것으로 판단된다.

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.937-946
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• 2004

Duroc is widely used to improve the meat quality and productivity. To elucidate the phylogenetic relation and the sequence specificity for the maternal property, the complete sequence of mitochondrial genome was determined and the population diversity of Duroc was investigated in this study. The length of mtDNA tested is 16,584-bp. There are several insertion/deletion mutations in the control region and coding regions for tRNA and rRNA, respectively, but not in peptide-coding regions. Four peptide-coding genes(COⅡ, COⅢ, ND3 and ND4) showed incomplete termination codon sequences such as T--, and two(ND2 and ND4L) did alternative initiation codons(AIC), respectively. Especially, the initiation codon sequences of ND2 gene were polymorphic in this population. Polymorphisms were detected in 11-bp duplication motif within control region as well as ND2 and CYTB. Variation patterns observed from the tests on three mtDNA regions were linked completely and then two haplotypes obtained from combining the data dividing this population. Duroc mtDNA is observed at the European pig cluster in the phylogenetic tree, however, the results from the population analyses supported previous opinions. This study suggests that the breed Duroc was mainly originated from the European pig lineage, and Asian lineage was also used to form the pig breed Duroc as maternal progenitors.

• Food Science of Animal Resources
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• v.34 no.6
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• pp.799-807
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• 2014

Meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. Nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. In this study, we designed Loop-mediated isothermal amplification (LAMP) assays targeting species-specific mitochondrial DNA to identify and discriminate eight meat species; cattle, pig, horse, goat, sheep, chicken, duck, and turkey. The LAMP primer sets were designed and the target genes were discriminated according to their unique annealing temperature generated by annealing curve analysis. Their unique annealing temperatures were found to be $85.56{\pm}0.07^{\circ}C$ for cattle, $84.96{\pm}0.08^{\circ}C$ for pig, and $85.99{\pm}0.05^{\circ}C$ for horse in the BSE-LAMP set (Bos taurus, Sus scrofa domesticus and Equus caballus); $84.91{\pm}0.11^{\circ}C$ for goat and $83.90{\pm}0.11^{\circ}C$ for sheep in the CO-LAMP set (Capra hircus and Ovis aries); and $86.31{\pm}0.23^{\circ}C$ for chicken, $88.66{\pm}0.12^{\circ}C$ for duck, and $84.49{\pm}0.08^{\circ}C$ for turkey in the GAM-LAMP set (Gallus gallus, Anas platyrhynchos and Meleagris gallopavo). No cross-reactivity was observed in each set. The limits of detection (LODs) of the LAMP assays in raw and cooked meat were determined from $10pg/{\mu}L$ to $100fg/{\mu}L$ levels, and LODs in raw and cooked meat admixtures were determined from 0.01% to 0.0001% levels. The assays were performed within 30 min and showed greater sensitivity than that of the PCR assays. These novel LAMP assays provide a simple, rapid, accurate, and sensitive technology for discrimination of eight meat species.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2016.11a
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• pp.102-102
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• 2016

본 연구에서는 직류 전원(direct current; DC)을 이용한 스퍼터링과 고전력펄스 마그네트론 스퍼터링(high-power impulse magentron sputtering; HIPIMS)의 두 가지 방법과 빗각 증착을 적용하여 제조한 티타늄 질화물(TiN) 박막의 미세구조 변화가 물성에 미치는 영향을 확인하였다. TiN 박막은 99.5%의 Ti 타겟을 사용하고, Ar가스와 $N_2$ 분위기에서 스테인리스(SUS304)와 초경(cdmented carbide; WC-10wt.%Co) 기판위에 코팅하였다. 기판은 알코올과 아세톤으로 초음파 세척을 실시한 후 진공용기에 장착하고 기본 진공도인 ${\sim}2.0{\times}10^{-5}Torr$ 까지 진공배기를 실시하였다. 기판과 타겟 간의 거리는 DC 스퍼터링은 10 cm, HIPIMS 스퍼터링은 8.5 cm 이었다. 진공용기의 압력이 기본 진공도까지 배기되면 Ar 가스를 ${\sim}10^{-2}Torr$로 주입한 후 기판에 라디오 주파수(radio frequency; RF) 전원으로 약 -800 V의 전압을 인가하여 글로우 방전을 발생시키고 약 30 분간 청정을 실시하였다. 기판의 청정이 끝난 후 기본 진공도까지 배기한 후 Ar와 $N_2$ 가스를 ${\sim}10^{-3}Torr$로 주입하여 TiN 코팅을 실시하였다. 빗각의 크기는 $45^{\circ}$와 $-45^{\circ}$이며, TiN 박막의 총 두께는 약 $2.5{\sim}4.0{\mu}m$ 로 유지하였다. 공정조건에 따라 TiN 박막의 주상정은 형태와 기울어진 각도가 다른 것을 확인하였다. DC 스퍼터링으로 제조된 TiN 박막은 기판홀더에 약 -100 V 의 bias 전압을 인가하면 인가하지 않은 박막에 비해 치밀한 박막의 성장과 경도 값도 증가하는 사실을 확인하였다. 또한 빗각을 적용하고 bias 전압을 인가하지 않은 시편에서 박리현상이 일어났다. HIPIMS로 제조한 TiN 박막은 bias 전압을 인가한 박막과 인가하지 않은 박막의 주상정 형상과 경도 값에 큰 차이가 없었으며, 박막의 박리현상은 모든 시편에서 일어나지 않았다. DC 스퍼터링으로 제조한 TiN 박막은 bias 전압을 인가하지 않으면 색상이 노란색이 아닌 갈색으로 나타났으며, HIPIMS으로 제조한 박막은 bias 전압 인가 유무에 상관없이 노란색 색상을 나타냈다. 앞서 설명한 DC 스퍼터링과 HIPIMS의 공정조건에 따라 나타난 박막의 경도, 색상, 물성변화 차이는 DC 스퍼터링보다 높은 HIPIMS의 이온화율에서 기인한 것으로 생각된다. 본 연구결과를 이용하면 다양한 형태의 박막 구조 제어가 가능하고 이러한 미세구조 제어를 통해서 박막의 물성도 제어가 가능할 것으로 판단된다.

• Archives of Pharmacal Research
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• v.16 no.3
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• pp.244-250
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• 1993

Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.10
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• pp.1205-1215
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• 2017

In this study, the hydrolysis rate of palm kernel oil (HPKO) and shea butter were compared by in vitro digestion to develop low-digestible fats. HPKO exhibited a higher hydrolysis rate than shea butter. The initial rate and ${\Phi}max$ value of HPKO were 0.315 mM/s and 78.0%, while the corresponding values for shea butter were 0.117 mM/s and 41.4%. When the two fats were blended at various ratios, the hydrolysis rate, in terms of the ${\Phi}max$ value, was similar to that of shea butter until 2:8 (HPKO : shea butter, w/w). After the analysis of triacylglycerol species and the positional fatty acid composition, the factors that affected the hydrolysis rate were determined. The results suggest that the low hydrolysis rate of shea butter would be due mostly to the stearic acid located at the sn-1,3 positions of triacylglycerol molecules. These properties of shea butter are expected to be the nutritional benefits as a low-digestible fat in foods.

• Korean Journal of Environment and Ecology
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• v.25 no.5
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• pp.658-665
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• 2011

An wild boar species which has been known as an extinct species on Jeju Island, was recently observed in the surrounding areas of Mount Halla. Based on the molecular techniques, this study examines whether they are crossbred with domesticated pig breeds. Intraspecific genetic relationships with other wild boar populations and molecular sexing were examined as well. Total of four molecular markers on mitochondrial DNA(control region and ND2) and nuclear DNA(MC1R and KIT) were applied to test crossbreeding between with domesticated pig breeds, such as Landrace, Large White, Berkshire, Hampshire, and Duroc. All individuals of wild boar population had identical mtDNA control region(CR) sequences. In addition, the sequences were the same as those of some native pig breeds which are distributed in Northeast China, but different from those previously reported from the Korean Peninsula up to date. These results suggest that this population may have originated from a genetic lineage had been not previously studied and genetically related to Chinese native pig breeds. Molecular sexing results show that there are twice as many females as male. Thus the population is under expansion and its size will dynamically increase if not controlled.

• Membrane Journal
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• v.8 no.4
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• pp.203-209
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• 1998

The cutting oil emulsion microfiltration was carried out on dead-end call and crossflow systems equipped with 0.22 $\mu$m GVHP Millipore and 0.2 m stainless steel Mott microfiltration membranes, respectivdy. The oil drop size in the emulsion was varied from 0.07 to 0.22 $\mu$m. Cake filtration(CFM) and standard pore blocking models(SPBM) were applied to predict the permeation flux. The permeation fluxes of 0.01 vol% oil emulsion followed CFM for dead-end system very well under the condition of 400 rpm and below 100 kPa. The SPBM was, however, suitable for the permeation flux at 400 rpm and above 150 kPa. The oil layer on the membrane surface was destroyed and reproduced repeatedly as operating pressure was suddenly changed from 60 to 200 kPa, and then returned to 60 kPa. Also, we estimated the critical entry pressure(CEP) which is changed from CFM to SPBM, and CEP for dead-end system was around 100 kPa. The CEP increased from around 100 to 150 kPa for the crossflow system as the oil concentration increased from 0.01 to 0.03 vol% when Reynolds number was 7080.

• Journal of Korea Water Resources Association
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• v.32 no.6
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• pp.751-762
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• 1999

In this study, a 1-D laboratory experiment was conducted to investigate the characteristics of transient unsaturated solute transport by using two kinds of soils of which properties were known by test. Especially the TDR method which is proposed in this study was used to measure water content and solute concentration. As results, in the transient flow, the wetting front moves down rapidly, and the distribution of solute concentration near the wetting front showed the similar type of the water content distribution(semi-bell type). A numerical model HYDRUS was used to compare with the experimental results. Numerical results for the water movement are similar to experimental result. However, numerical results of the distribution of solute concentration are more scattered than experimental results. It means that measured dispersivity, numerical dispersion, adsorption coefficient, and soil sample size etc. should be considered in order to determine the dispersivity used in the numerical model. The present measuring method was proved to be superior to other formula and to be an available method to apply to solute transport test. The measuring error of the developed method is estimated smaller than 10% while water content is larger than 0.15.