• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.239-244
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• 2012

This study investigated the hypoglycemic effect of the Padina arborescens extract in STZ-induced diabetic mice. Freeze-dried Padina arborescens were extracted with 80% methanol and concentrated for use in this study. The hypoglycemic effect was determined by inhibitory activities against ${\alpha}$-glucosidase and ${\alpha}$-amylase as well as the alleviation of postprandial blood glucose level. Padina arborescens extracts showed higher inhibitory activities than acarbose, a positive control against ${\alpha}$-glucosidase and ${\alpha}$-amylase. The $IC_{50}$ values of Padina arborescens extracts against ${\alpha}$-glucosidase and ${\alpha}$-amylase were 0.26 and 0.23 mg/mL, respectively, which evidenced as more effective than observed with acarbose. The increase of postprandial blood glucose levels were significantly suppressed in the Padina arborescens extract administered group than the control group in the streptozotocin induced diabetic mice. Furthermore, the area under the curve (AUC) was significantly lowered via Padina arborescens extract administration in diabetic mice (p 0.05). These results indicated that the Padina arborescens extract might be used as an inhibitor of ${\alpha}$-glucosidase and ${\alpha}$-amylase and delay absorption of dietary carbohydrates.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.141.3-142
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• 2000

• 생명과학회지
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• 제20권7호
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• pp.1012-1018
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• 2010

본 연구에서, 고지방식이에 의한 비만, streptozotocin처리에 의한 당뇨 및 노화에 의해 알츠하이머병의 원인 유전자인 APP의 발현이상이 생기는지를 조사하였다. 일반식이(ND)/고지방식이(HFD), 16주령/26주령, 및 정상/당뇨 등의 조건별 8가지 조합에 88마리의 C57BL/6 생쥐를 각 그룹에 최소 10마리씩 나누어 키웠으며, 각 실험생쥐로부터 추출한 부고피하지방 및 부고환지방조직에서의 APP mRNA 발현양을 quantitative real-time PCR로 측정하였다. 그 결과, 피하지방조직에서의 APP 유전자는 16주령과 26주령에서 고지방식이로 키워 비만을 유도한 동물에서 2배정도 높게 발현되었으나(16주령 $125.0{\pm}13.9$ vs. $63.5{\pm}9.9$, p=0.001; 26주령 $120.2{\pm}6.0$ vs. $51.8{\pm}6.3$, p<0.0001), 부고환지방조직에서는 APP 발현양의 차이가 나타나지 않았다. 16주령과 26주령 사이에서의 노화에 따른 APP 발현양의 차이도 나타나지 않았다. 또한, 일반 및 고지방식이로 키운 16주령 생쥐의 피하지방조직 APP 유전자 발현은 STZ에 의해 유도된 당뇨병 생쥐에서 크게 감소되었다(ND non-diabetic $63.5{\pm}9.9$ vs. diabetic $40.2{\pm}5.0$, p<0.05; HFD $125.0{\pm}13.9$ vs. $67.0{\pm}9.0$, p<0.01). APP mRNA 발현양의 선형회귀분석에 의하면, 당뇨병이 유발되지 않은 일반 생쥐(R=0.657, p<0.0001, n=39)와 당뇨병 생쥐(R=0.508, p=<0.0001, n=49) 모두에서의 APP mRNA 발현양이 체중과 깊은 연관성이 있음이 확인되었으나, APP mRNA 양과 혈당과의 연관성은 나타나지 않았다. 이는 STZ에 의한 당뇨병 생쥐에서 관찰된 APP mRNA 발현의 감소는 고혈당증에 의한 것이 아닌 체중감소의 영향인 것으로 추측된다. 본 연구의 결과로 APP mRNA는 생쥐의 피하지방 및 부고환지방에서 발현되고 있고, 비만에의 의해 증가하며, 체중감소에 의해 감소한다는 것을 확인하였다. 이들 결과는 사람에게서 확인된 체중변화에 의한 APP 이상발현 현상을 더욱 분명히 보여주었으며, 이런 APP 이상발현이 중년기 비만과 노령기의 알츠하이머병 환자 뇌에서의 amyloidogenesis 증가 매개자로서의 역할 가능성을 시사한다.

• 대한약침학회지
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• 제25권2호
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• pp.130-137
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• 2022

Objectives: Insulin resistance (IR) is major cause of type 2 diabetes (T2D), and adipokines (e.g., adiponectin, leptin, and resistin) play an important role in insulin sensitivity. Medicinal plants are frequently used for T2D treatment. This study investigates the effect of Artemisia annua L. (AA) extracts on adipokines in mice with high-fat-diet (HFD)/streptozotocin (STZ)-induced T2D. Methods: We divided 60 mice into 12 groups (n = 5 per group): control, untreated T2D, treated T2D, and 9 other groups. T2D was induced in all groups, except controls, by 8 weeks of HFD and STZ injection. The treated T2D group was administered 250 mg/kg of metformin (MTF), while the nine other groups were treated with 100, 200, and 400 mg/kg of hot-water extract (HWE), cold-water extract (CWE), and alcoholic extract (ALE) of AA (daily oral gavage) along with 250 mg/kg of MTF for 4 weeks. The intraperitoneal glucose tolerance test (IPGTT) was performed, and the homeostasis model assessment of adiponectin (HOMA-AD) index and blood glucose and serum insulin, leptin, adiponectin, and resistin levels were measured. Results: Similar to MTF, all three types of AA extracts (HWEs, CWEs, and ALEs) significantly (p < 0.0001) decreased the area under the curve (AUC) of glucose during the IPGTT, the HOMA-AD index, blood glucose levels, and serum insulin, leptin, and resistin levels and increased serum adiponectin levels in the MTF group compared to the T2D group (p < 0.0001). The HWEs affected adipokine release, while the CWEs and ALEs decreased leptin and resistin production. Conclusion: Water and alcoholic AA extracts have an antihyperglycemic and antihyperinsulinemic effect on HFD/STZ diabetic mice. In addition, they decrease IR by reducing leptin and resistin production and increasing adiponectin secretion from adipocytes.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.205.2-206
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• 2003

This experiment was designed to evaluate the immune responses after treatment of diabetes by using water extract of traditional herbal drugs on the splenocytes and peritoneal macrophages in vivo. We found two herbal materials of the hypoglycemic agents based on inhibitory activity of ${\alpha}$-glucosidase. These potential herbal drugs which remarkably inhibited ${\alpha}$-glucosidase in STZ-induced diabetic mices (STZ 150 mg/kg, i.p.) were Mori radicis Cortex(MRC, 2.32 mg/mouse) and Cudraniae radicis Cortex (CRC, 2.24mg/mouse). (omitted)

• BMB Reports
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• 제55권11호
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• pp.559-564
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• 2022

Diabetes mellitus is one of the most prevalent diseases in modern society. Many complicationssuch as hepatic cirrhosis, neuropathy, cardiac infarction, and so on are associated with diabetes. Although a relationship between diabetes and hair loss has been recently reported, the treatment of diabetic hair loss by Wnt/β-catenin activators has not been achieved yet. In this study, we found that the depilation-induced anagen phase was delayed in both db/db mice and high-fat diet (HFD) and streptozotocin (STZ)-induced diabetic mice. In diabetic mice, both hair regrowth and wound-induced hair follicle neogenesis (WIHN) were reduced because of suppression of Wnt/β-catenin signaling and decreased proliferation of hair follicle cells. We identified that KY19382, a small molecule that activates Wnt/β-catenin signaling, restored the capabilities of regrowth and WIHN in diabetic mice. The Wnt/β-catenin signaling activator also increased the length of the human hair follicle which was decreased under high glucose culture conditions. Overall, the diabetic condition reduced both hair regrowth and regeneration with suppression of the Wnt/β-catenin signaling pathway. Consequently, the usage of Wnt/β-catenin signaling activators could be a potential strategy to treat diabetes-induced alopecia patients.

• 동의생리병리학회지
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• 제16권6호
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• pp.1253-1259
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• 2002

This study was carried out to investigate the effects of Bambusae Caulis in Liquamen from different production process on blood sugar of the diabetic mice induced with Streptozotocin(STZ). The original Bambusae Caulis in Liquamen filtered and refined. Bambusae Caulis in Liquamen D(H-BCL.D) extracted at high temperature(1000℃), Bambusae Caulis in Liquamen A(L-BCL.A) and Bambusae Caulis in Liquamen B(L-BCL.B) extracted at low temperature (250～450℃) were administerd to mice for 4weeks and its anti-diabetic effect examined. Mice used in this experiment were divided into four groups(Control, H-BCL.D, L-BCL.A and L-BCL.B). Experemental groups were observed in terms of blood sugar, Creatinine, BUN and GPT. The amount of glucose was significantly decreased in the Bambusae Caulis in Liquamen-treated groups compared with the control(P < 0.05). The amount of Creatinine did not show any differences among four groups. The amount of Blood Urea Nitrogen did not show any differences in the L-BCL.A and B-treated groups, but did show slightly decrease(P<0.05) at H-BCL.D-treated group. The amount of GPT did not show any differences among four groups. In conclusion, it was found that Bambusae Caulis in Liquamen from extracted at high or low temperature were effective on murine hyperglycemia mice induced with STZ respectively.

• 한국식품과학회지
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• 제49권5호
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• pp.550-558
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• 2017

본 연구는 쑥 추출물(AA)과 홍국균(Monascus purpureus)으로 발효된 쑥(AAFM)을 이용하여 STZ로 인한 당뇨성 인지장애 개선효과를 확인하고자 수행되었다. C57BL/6L 마우스에 streptozotocin(STZ)을 복강 주사하여 당뇨를 유발시킨 후 4개의 군으로 분리하였고, 6주간 24시간 간격으로 AA 10 mg/kg BW와 AAFM 10 mg/kg BW을 경구투여 하였다. 7일 간격으로 혈당을 측정하였으며, 혈당 측정 결과 AA와 AAFM을 섭취한 군에서 STZ군 대비 유의적인 혈당감소를 나타내었다. Passive avoidance, Morris water maze 및 Y-maze test를 통해 STZ로 유도한 당뇨 동물모델에서 기억력 장애를 확인하였으며, 발효 쑥(AAFM)군에서 학습에 의한 기억력과 운동능력이 더 회복된 것을 확인하였다. 혈청분석 결과 STZ군에서는 GOT, GPT, BUN와 혈중 지질 함량이 높게 나타난 반면 발효 쑥(AAFM)군에서는 GOT, GPT 및 BUN이 STZ그룹에 비해 유의적으로 감소하였으며, HTR이 높게 나타났고 TG와 LDLC가 낮게 나타나 혈중 지질 성분이 개선됨을 확인할 수 있었다. 마우스 뇌 조직에서 발효 쑥(AAFM)은 아세틸콜린에스터레이스 활성을 저해시키고 아세틸콜린 함량을 증가시킴으로써 STZ로 인한 당뇨병에서의 신경전달시스템을 개선효과를 나타내었다. 발효 쑥(AAFM)의 경우, 마우스의 뇌 조직에서 SOD 함량을 증가시키고, 뇌와 간에서의 MDA 함량을 감소 시킴으로써 STZ와 같은 산화적 스트레스 인자에 대한 산화방지 효과를 나타내었다. 이러한 결과들을 종합해볼 때, 홍국균을 활용한 발효 쑥(AAFM)은 STZ로 유도된 당뇨 모델에서 당뇨성 인지장애에 대한 기억력 개선효과를 나타내었다. 특히, 쑥 추출물(AA)보다 마우스의 운동능력을 개선시켰으며, 혈중 지질 개선효과를 나타냈다. 더불어 산화방지 효과를 나타내 고혈당으로 발생될 수 있는 산화적 스트레스로부터 뇌 조직 등을 보호할 수 있음을 확인하였다. 결국 고혈당으로 인한 인지장애의 예방과 치료에도 효과를 나타낼 것으로 사료되어 고부가가치 식품으로 활용될 수 있는 산업적 가능성이 있다고 판단된다.

• 동의생리병리학회지
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• 제24권6호
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• pp.1012-1018
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• 2010

This study investigated the functional effect by diets of Triticum aestivum sprout (TA) on the levels of blood glucose and lipid profiles in streptozotocin(STZ)-induced diabetic mice. Diabetes mellitus were induced by STZ intraperitoneal injection (50 mg/kg) into the male Balb/c mice. All mice fed AIN-93 diet for 3 weeks. Mice were divided to 4 groups: normal, diabetic control and two experimental groups (TA 5.0, diet with Triticum aestivum sprout 5.0% w/w; TA 2.5, diet with Triticum aestivum 2.5% w/w). The blood glucose level was decreased by TA intake in diabetic mice in the dose-dependant manner; 21.34% and 35.77% in TA 2.5 and TA 5.0 groups. The weight gain and feed efficiency ratio also were improved by TA intake compared with DM group. The weight gain was increased 1.28 and 1.09 folds in TA 5.0 and TA 2.5 groups, respectively. The weight of organs, including liver and kidney, was reduced but that of spleen and abdominal fat was increased by TA intake. Moreover, TA treatment increased the level of HDL but decreased the level of total cholesterol and triglycerides in the blood of diabetic mice. The blood of HbA1c also was significantly decreased without changing hematocrit by TA treatment compared with DM group. These results indicate the TA intake has the functional effects attenuating blood glucose and plasma lipid levels in insulin-dependent diabetes.

• Journal of Nutrition and Health
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• 제56권1호
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• pp.24-34
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• 2023

상지 물추출물의 투여는 STZ 유도 당뇨 마우스의 공복 혈당, 혈중 총 CHO, LDL-CHO, 간 무게 및 간 조직 TG를 NC군 수준으로 감소시키고 혈장 총 CHO대비 HDL-CHO비는 NC군 수준으로 증가시켰으며, 소장 proximal region의 이당류 분해효소인 lactase, sucrase 활성을 억제하였다. 이는 상지를 차로 우려내어 음용할 경우 당뇨 예방 및 증상 개선의 가능성을 기대해 볼 수 있음을 제시한다. 상지는 국내산 뽕나무 부산물로 대량 생산될 뿐만 아니라 식품의 원료로 사용이 가능한 소재이므로 향후 상지 물추출물의 생리활성성분 분리 및 혈당조절기전 연구를 보다 깊이 수행한다면 안전하고 효과적인 고부가가치 혈당조절 기능성식품 개발의 기초자료로 활용될 수 있을 것이다.