• Title/Summary/Keyword: STR

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채널정보 기반 OFDMA FD-MAC 프로토콜 (OFDMA FD-MAC Protocol Based on the Channel Information)

  • 천혜림;김재현
    • 한국통신학회논문지
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    • 제40권3호
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    • pp.451-458
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    • 2015
  • 무선랜 이용률 증가에 따라 무선랜 단말뿐만 아니라 AP의 밀집도가 증가하고 있고, 이로 인해 무선랜의 실제 성능(real performance)이 저하되고 있다. 이를 개선하기 위한 PHY/MAC 기술 중 OFDMA와 full duplex가 있다. 기존 OFDMA기반 무선랜 MAC 연구는 채널 utilization과 충돌확률 측면에서의 문제점이 있고 따라서 보다 효율적인 채널할당 프로토콜에 대한 연구가 필요하다. Full duplex는 같은 주파수에 동시에 송수신이 가능한 방식으로 half duplex에 비해 더 유연한 채널할당이 가능하다. 본 논문에서는 full duplex 전송 노드의 idle 채널정보를 바탕으로 서브 채널을 할당하는 OFDMA FD-MAC(Full Duplex MAC) 프로토콜을 제안하였다. 또한, 성능분석을 통해 throughput이 개선됨을 확인하였다.

Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells

  • Shin, Dong-Hyuk;Lee, Jeoung-Eun;Eum, Jin Hee;Chung, Young Gie;Lee, Hoon Taek;Lee, Dong Ryul
    • 한국발생생물학회지:발생과생식
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    • 제21권4호
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    • pp.425-434
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    • 2017
  • Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and examined whether it could be available as a research model for the polyploidy cells existed in the human tissues. Two tetraploid hESC lines were artificially acquired by reintroduction of remained 1st polar body during the establishment of SCNT-hESC using MII oocytes obtained from female donors and dermal fibroblasts (DFB) from a 35-year-old adult male. These tetraploid SCNT-hESC lines (CHA-NT1 and CHA-NT3) were identified by the cytogenetic genotyping (91, XXXY,-6, t[2:6] / 92,XXXY,-12,+20) and have shown of indefinite proliferation, but slow speed when compared to euploid SCNT-hESCs. Using the eight Short Tendem Repeat (STR) markers, it was confirmed that both CHA-NT1 and CHA-NT3 lines contain both nuclear and oocyte donor genotypes. These hESCs expressed pluripotency markers and their embryoid bodies (EB) also expressed markers of the three embryonic germ layers and formed teratoma after transplantation into immune deficient mice. This study showed that tetraploidy does not affect the activities of proliferation and differentiation in SCNT-hESC. Therefore, tetraploid hESC lines established after SCNT procedure could be differentiated into various types of cells and could be an useful model for the study of the polyploidy cells in the tissues.

비대칭 마그네트론 스퍼터링법에 의해 합성된 STR304 스테인리스강 박막에서의 질소와 산소의 첨가 효가 (Effect of $N_2$ and $O_2$ Properties of STS304 Stainless Steel Films Synthesized by Unbalanced Magnetron Sputtering Process)

  • 김광석;이상율;김범석;한전건
    • 한국표면공학회지
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    • 제34권2호
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    • pp.89-96
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    • 2001
  • N- or O-doped STS304 stainless films were synthesized by an unbalanced magnetron sputtering process with various argon and reactive gas ($N_2$, $O_2$) mixtures. These films were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), Auger electron spectroscopy (AES) and Knoop microhardness tester. The Results from X-ray diffraction (XRD) analysis showed that a STS304 stainless steel film synthesized without reactive gas using a bulk STS304 stainless steel target had a ferrite bcc structure ($\alpha$ phase), while the N-doped STS304 stainless film was consisted of a nitrogen supersaturated fcc structure, which hsa a strong ${\gamma}$(200) phase. In the O-doped films, oxide Phases ($Fe_2$$O_3$ and $Cr_2$$O_3$) were observed from the films synthesized under an excess $O_2$ flow rate of 9sccm. AES analysis showed that nitrogen content in N-doped films increased as the nitrogen flow rate increased. Approximately 43 at.%N in the N-doped film was measured using a nitrogen flow rate of 8sccm. In O-doped film, approximately 15 at.%O was detected using a $O_2$ flow rate of 12sccm. the Knoop microhardness value of N-doped film using a nitrogen flow rate of 8 sccm was measured to be approximately $H_{ k}$ 1200 and this high value could be attributed to the fine grain size and increased residual stress in the N-doped film.

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인삼 유청음료의 제조 및 관능적 특성 (Ginseng-Whey Beverage Production and Sensory Properties)

  • 기해진;홍윤호
    • 한국식품영양과학회지
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    • 제22권2호
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    • pp.202-207
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    • 1993
  • 렌넷유청, 인삼, 감미료, 꿀, 매실 등에 종류가 다른 유산균주들을 접종하여 인삼 유청음료를 제조한 후, 일부는 발효시키고 일부는 비발효 상태로 4$^{\circ}C$ 및 30$\pm$1$^{\circ}C$에 저장하여 1, 3, 5주에 관능검사를 실시하였다. 관능검사 결과, 감미료와 꿀을 첨가하여 냉장보관 한 유산균 비발효 인삼 유청음료(A)는 단맛이 강하고 신맛, 쓴맛, 떫은맛, 뒷맛이 적어서 전체적인 평가에서 8.64~8.85으로 가장 높은평가를 받았고 Lac. caseisub-sp. casei와 Str. salivarius sbu-sp. thermophilus를 접종하여 냉장보관한 인삼 유청음료 (C)와 감미료, 꿀 및 매실을 첨가하여 실온보관한 인삼 유청음료(D)가 대체로 좋은 평가를 받았으나 실온보관한 유산균 발효 음료(E, F)는 2.92~3.58로 가장 낮은 평가를 받았으므로 이의 개선이 요구된다.

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Genotyping of avian pathogenic Escherichia coli by DNA fragment analysis for the differences in simple sequence repeats

  • Han, Mi Na;Byeon, Hyeon Seop;Han, Seong Tae;Jang, Rae Hoon;Kim, Chang Seop;Choi, Seok Hwa
    • 한국동물위생학회지
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    • 제41권4호
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    • pp.257-262
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    • 2018
  • Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

Complete mitochondrial genome of Rotunda rotundapex Miyata & Kishida 1990 (Lepidoptera: Bombycidae), which was named as Bombyx shini Park & Sohn 2002

  • Park, Jeong Sun;Kim, Min Jee;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제44권2호
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    • pp.55-64
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    • 2022
  • Bombyx shini Park & Sohn, 2002 (Lepidoptera: Bombycidae), which was listed as an endemic species in South Korea has recently been renamed as the East Asian silk moth Rotunda rotundapex Miyata & Kishida, 1990 (Lepidoptera: Bombycidae). In this study, we sequenced the complete mitochondrial genome (mitogenome) of the R. rotundapex to announce genomic characteristics and to clarify its validity with a new name. The 15,294-bp long complete mitogenome comprises a typical set of genes [13 protein-coding genes (PCGs), 2 rRNA genes, and 22 tRNA genes] and one major noncoding, A + T-rich region, with an arrangement identical to that observed in most lepidopteran mitogenomes. The A/T content of the whole mitogenome was 79.22%; however, it varied among the regions/genes as follows: A + T-rich region, 91.62%; srRNA, 84.67%; lrRNA, 83.01%; tRNAs, 81.43%; and PCGs, 77.46%. Phylogenetic analyses of 35 species in the Bombycoidea superfamily showed the sister relationship between the families Sphingidae and Bombycidae s. str., with the higher nodal support [bootstrap support (BS) = 78%]. The Saturniidae was placed as the sister to the two families, but the nodal support for this relationship was low (BS = 53%). Current R. rotundapex was placed together with previously reported con-species with the highest nodal support, forming a separate clade from Bombyx, validating that B. shini can have a new genus name, Rotunda. However, the Korean R. rotundapex showed a substantial sequence divergence at 5.28% to that originated from an individual of type locality Taiwan in 1,459-bp of COI sequences. Considering such a high sequence divergence an additional study, which includes morphological and DNA barcoding data from further extensive distributional range maybe is needed for further robust taxonomic conclusion.

소와 돼지 도축장 도체 및 환경에서 분리된 병원성대장균 분포 및 특성조사 (Prevalence and characterization of pathogenic Escherichia coli from carcasses and environmental samples of cattle and pig slaughterhouses)

  • 홍세림;강혜정;문진산;윤순식;김하영
    • 한국동물위생학회지
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    • 제45권3호
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    • pp.191-199
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    • 2022
  • We investigated the virulence genes, O-serotypes, antimicrobial resistance of pathogenic E. coli isolated from carcasses (n=455) and environmental (n=372) samples of 11 cattle and 12 pig slaughterhouses from December 2020 to December 2021. E. coli were isolated from nine carcasses (2.0%), three slaughter facilities (1.4%), two utensils (2.7%) and three abattoir workers (3.5%) from four cattle and four pig slaughterhouses. Among all isolates, 13 STEC (76.5%) were identified, followed by four EPEC (23.5%). As a result of the antibiotic susceptibility test, all isolates were resistant to at least one antimicrobial, of which 70.6% isolates showed multidrug resistance patterns. The serotypes were diverse in pigs compared to cattle, with serotypes O18, O66, O109 in cattle and O9, O76, O85, O100, O153, and O159 in pigs. In a single cattle slaughterhouse, eight STEC O66 were isolated from various types of sample (4 slaughter animal surfaces, 3 gloves, and 1 knife) with two antimicrobial resistance patterns (CHL-FIS-STR and CHL-FIS). Those two types of strain were suspected cross-contamination from utensils to slaughter animal surfaces. These results showed that pathogenic E. coli were detected in carcasses and various environmental samples in cattle and pig slaughterhouses. Nationwide monitoring and hygiene management are required to prevent cross-contamination of STEC isolate slaughterhouses.

Vibration behavior of partially bio-sourced sandwich panel with orthogonally stiffened core: Analytical and experiment study

  • Boussoufi, Aicha;Errouane, Lahouaria;Sereir, Zouaoui;Antunes, Jose V.;Debut, Vincent
    • Advances in aircraft and spacecraft science
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    • 제9권3호
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    • pp.169-193
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    • 2022
  • By the present paper, both experimental and analytical models have been proposed to study the vibration behavior of partially bio-sourced sandwich panel with orthogonally stiffened core. For a variable mass fraction of Alfa fibers from 5% to 15%, impregnated in a Medapoxy STR resin, this panel were manufactured by molding the orthogonally stiffened core then attached it with both skins. Using simply supported boundary conditions, a free vibration test was carried out using an impact hammer for predicting the natural frequencies, the mode shapes and the damping coefficient versus the fibers content. In addition, an analytical model based on the Higher order Shear Deformation Theory (HSDT) was developed to predict natural frequencies and the mode shapes according to Navier's solution. From the experimental test, we have found that the frequency increases with the increase in the mass fraction of the fibers until 10%. Beyond this fraction, the frequencies give relatively lower values. For the analytical model, variation of the natural frequencies increased considerably with side-to-thickness ratio (a/H) and equivalent thickness of the core to thickness of the face (hs/h). We concluded that, the vibration behavior was significantly influenced by geometrical and mechanical properties of the partially bio-sourced sandwich panel.

갓과 흑미강 혼합물의 항산화 활성 및 지방세포 억제 효과 (Antioxidant Activity and Anti-Obesity Effects of Mixture of Brassica juncea and Black Rice Bran)

  • 임지현;임준석;한웅호;문효;오건;조근희;최선일;황운상;이옥환
    • 한국식품영양학회지
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    • 제36권4호
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    • pp.244-252
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    • 2023
  • This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

컴패니언 로봇의 멀티 모달 대화 인터랙션에서의 감정 표현 디자인 연구 (Design of the emotion expression in multimodal conversation interaction of companion robot)

  • 이슬비;유승헌
    • 디자인융복합연구
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    • 제16권6호
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    • pp.137-152
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    • 2017
  • 본 연구는 실버세대를 위한 컴패니언 로봇의 인터랙션 경험 디자인을 위해 사용자 태스크- 로봇 기능 적합도 매핑에 기반한 로봇 유형 분석과 멀티모달 대화 인터랙션에서의 로봇 감정표현 연구를 수행하였다. 노인의 니즈 분석을 위해 노인과 자원 봉사자를 대상으로 FGI, 에스노그래피를 진행하였으며 로봇 지원 기능과 엑추에이터 매칭을 통해 로봇 기능 조합 유형에 대한 분석을 하였다. 도출된 4가지 유형의 로봇 중 표정 기반 대화형 로봇 유형으로 프로토타이핑을 하였으며 에크만의 얼굴 움직임 부호화 시스템(Facial Action Coding System: FACS)을 기반으로 6가지 기본 감정에 대한 표정을 시각화하였다. 사용자 실험에서는 로봇이 전달하는 정보의 정서코드에 맞게 로봇의 표정이 변화할 때와 로봇이 인터랙션 사이클을 자발적으로 시작할 때 사용자의 인지와 정서에 미치는 영향을 이야기 회상 검사(Story Recall Test: STR)와 표정 감정 분석 소프트웨어 Emotion API로 검증하였다. 실험 결과, 정보의 정서코드에 맞는 로봇의 표정 변화 그룹이 회상 검사에서 상대적으로 높은 기억 회상률을 보였다. 한편 피험자의 표정 분석에서는 로봇의 감정 표현과 자발적인 인터랙션 시작이 피험자들에게 정서적으로 긍정적 영향을 주고 선호되는 것을 확인하였다.