• Title/Summary/Keyword: STR

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The Spider Fauna of lsl. Ulreng-do (Dagelt), Korea (울릉도의 거미상)

  • Namkung Joon;Paik Nam-keuk;Yoon kyung-il
    • Korean journal of applied entomology
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    • v.20 no.1 s.46
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    • pp.51-58
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    • 1981
  • Hitherto 44 species belonging to 19 families of spiders have been reported at Ulreung-do on the East Sea. Korea. The authors have investigated spider fauna, through out the Ulreung-do, during July 25, August 3. 1975 and July 30, August 7, 1980. The foundings were as followings. 1) In this report, 132 species of 74 genera, including 3 undetermined species, belonging to 25 families of spiders are tabulated by the aid of published data and collections of the authors. Eighty six species are added to the fauna, of which 2 species are new to Korea. as follows; Porrhomma montanum Jackson. 1918, Clubiona neglecta O.P. Cambridge, 1862. The spider fauna of Ulreung-do includes 18 nothern species $(14.0\%)$, 8 southern species $(6.2\%)$. 7 cosmopolitan species $(5.4\%)$ and 84 Palaearctic temperate species $(65.1\%)$. The predominant species are Araneids. Thridiids, especially Cyclosa japonica Boes. et Str. are found in large numbers all over this island. Linyphiids and Lycosids are relatively few in number. 3) The common species to comparative area and foreign countries are as follows; Ulreung-do/Jookbyeon...76 species$(43.3\%)$ Ulreung-do/Jeju-do...95 species$(40.6\%)$ Ulreung-do/Heuksan do...61 species$(35.7\%)$ Ulreung-do/Deogjeog-do...51 species$(31.7\%)$ Ulreung-do/Tsushima Isl....71 species$(30.3\%)$ Ulreung-do/Japan...112 species$(11.3\%)$ Ulreung-do/main China...59 species $(6.5\%)$ 4) The ecological distribution are as follows; The seashore region...81 species$(62.8\%)$ The evergreens region...73 species$(56.6\%)$ The highiands region...64 species$(49.6\%)$ The valleys region...49 species$(38.0\%)$ The basin region...23 species$(17.8\%)$ 5) The noticeable one of geographical distribution in this island, are as follows; Nesticus quelpartensis Paik et Namkung, 1969, Conoculus simboggulensis Paik, 1971.

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Physiological Properties of Extracts of Traditional Soybean Doenjang Prepared with Korean Herb Medicines (국산 한약재 추출물을 이용한 전통 콩된장의 생리활성)

  • Park Seok-Kyu;Jeong Hoe-Jeong;Kim Hong-Chul;Lee Sang-Won
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.241-245
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    • 2006
  • The biofunctional properties of traditional Doenjang supplemented with the extract of herb medicines (refer as DHM) were examined DHM divided to four groups, I, II, III and IV according to herb medicines were added. Nitrite scavenging-activities from all 4 groups of DHMs were significantly higher than that of control. Also hydrogen donating-activities from all 4 groups of DHMs were slightly higher than that of control. Antibacterial activities against pathogenic bacteria such as Streptococcus mutant and Salmonella enteritidis and fibrinolytic activity of all DHMs on fibrin plate were higher than those of control. On the other those of groups III and IV were higher in sensory score, biofunctional and antimicrobial activities than those of group I, II and control. Therefore, the usage of extract of Korean herb medicines instead of water to make Doenjang will effective in development of traditional soybean Doenjang with the biofunctional properties.

Determination of Plasmids Encoding Crystal Toxic Protein Gene in Bacillus thuringiensis var kurstaki HD-1 (Bacillus thuringiensis var kurstaki HD-1의 내독소 단백질 유전에 관여하는 plasmid의 결정)

  • 김철영;김상현
    • Journal of Sericultural and Entomological Science
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    • v.35 no.2
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    • pp.120-128
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    • 1993
  • The objective of this study is to identify plasmids of Bacillus thuringiensis var. kurstaki HD-1(B. t k HD-1) toxic to lepidopteran larvae. The results from agarose gel electrophoresis indicated that the bacterium contained 9 plasmids with approximate sizes of 1.4, 4.9, 5.4, 9.3, 10, 29, 44, 52, and 150 megadaltons(Md). By treating the wild type of B. t k HD-1 with either SDS or EtBr as curing agent, 26 cured mutants of the bacterium were obtained, 9 of them were crystallifereous(cry+) and the others acrystallifereous(cry-). Plasmids from B. t k HD-1 were transferred to B. cereus 569 strR cry- recipients(Bc569 M1). Among 13 isolates of Bc569 M1 transcipient, 11 of them were capable of producing the crystal toxic proteins. The plasmid patterns of Bc569 M1 transcipients and partially curved mutants of B. t k HD-1 on agarose gel electrophoresis suggested that the 29 and 44Md plasmids should be involved in the production of crystalline toxic proteins.

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Effect of Temperature and Salt Concentration on Kimchi Fermentation (김치발효에 미치는 온도 및 식염농도의 영향)

  • Mheen, Tae-Ick;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.443-450
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    • 1984
  • Chemical and microbial changes during Kimchi (a group of Korean seasoned pickles) fermentation were carried out at various temperatures and salt concentrations. The time reaching optimum ripening of Kimchi varied depending upon fermentation temperature and salt concentration. At high temperature and low salt content Kimchi fermentation was faster than at low temperature and high salt content. The ratio of volatile to non-volatile acids reached its maximum at the optimum ripening time of Kimchi and decreased thereafter. Leu. mesenteroids, Lac. brevis, Lac. plantarum, Ped. cerevisiae, Str. faecalis and low acid producing Lactobacilli were isolated from Kimchi samples. However, the main microorganism responsible for Kimchi fermentation was Leu. mesenteroides and Lac. plantarum was the main acidifying organism. Total viable count increased rapidly in the beginning of fermentation and reached its maximum number at optimum ripening time and then decreased slowly as the acidity of Kimchi increased. While the total aerobic bacteria and fungi decreased during Kimchi fermentation, the yeast increased significantly at lower temperature.

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An Overview of Information Processing Techniques for Structural Health Monitoring of Bridges (교량 건전성 모니터링을 위한 정보처리기법)

  • Lee, Jong-Jae;Park, Young-Soo;Yun, Chung-Bang;Koo, Ki-Young;Yi, Jin-Hak
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.21 no.6
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    • pp.615-632
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    • 2008
  • The bridge health monitoring has become an important research topic in conjunction with damage assessment and safety evaluation of structures owing to the improvement of structural modeling techniques incorporating response measurements and the advancements in signal analysis and information processing capabilities. The bridge monitoring systems are generally composed of hardwares such as sensors, data acquisition equipment, data transmission systems, etc, and softwares such as signal processing, damage assessment, display and management, etc. In this paper, the research and development(R&D) activities on the information processing for structural health monitoring of bridges are reviewed. After a brief introduction to the process of bridge health monitoring, various information processing techniques including various signal processing and damage detection algorithms are introduced in detail. Several challenges addressing critical issues in the current bridge health monitoring system and future R&D activities are discussed.

Analysis of the antibiotic resistance gene in Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province (경북지역 환돈 유래 Salmonella Typhimurium의 약제내성 유전자 분석)

  • Kim, Joo-Hyung;Kim, Seong-Guk;Kim, Seon-Soo;Kim, Jeong-Hwa;Park, Sye-Hee;Nam, Ki-Hu;Kim, Hyoung-Bae
    • Korean Journal of Veterinary Service
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    • v.36 no.2
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    • pp.73-78
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    • 2013
  • This study was conducted to investigate antibiotic resistance among Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province during the period 1998~2011. One hundred forty one isolates were tested for antibiotic resistance using the standard disk diffusion method and were examined for presence of resistance gene by PCR method. S. Typhimurium showed high drug resistance rates to tetracycline (95.7%), streptomycin (93.6%), ampicillin (86.5%), cephalothin (80.1%), gentamicin (79.4%), and trimethoprim/sulfamethoxazole (72.3%). Resistance gene, blaTEM, blaPSE1, tetA, tetB, tetG, sul1, sul2, aadA, strA, grm, and temA were detected among the antibiotic resistance isolates and temB, tetC, aadB gene were not detected. One hundred twenty one (89.6%) tetA, two (1.5%) tetB and one (0.7%) tetG gene were detected in the 135 tetracycline resistant isolates. Two (1.6%) temA gene were detected in one hundred twenty two ampicillin resistance isolates and temB was not detected.

Effect of Fertilization Promoting Peptide on Kinematic Parameters, Capacitation and Acrosome Reaction in Human Spermatozoa (Fertilization Promoting Peptide가 사람 정자의 운동양태, 수정능력획득 및 첨체반응에 미치는 영향)

  • Kang, Hee-Gyoo;Kim, Myo-Kyung;Kim, Dong-Hoon;Han, Sung-Won;Choi, Do-Hyun;Lee, Ho-Joon;Kim, Moon-Kyoo
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.2
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    • pp.201-209
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    • 2000
  • Objective: This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa. Methods: After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, using the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis. Results: We have obtained the evidence that FPP can promote the capacitation and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. FPP ($25{\sim}100$ nM) induced a significant increase in the proportion of B-pattem capacitated spermatozoa, and a significant decrease in the proportion of F-pattem uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maint3ined higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.

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Imporoved Method for the Preparation of Silk Fibroin Hyoysates

  • Shukhrat Madyarov;Lee, kawng-Gill;Yeo, Joo-Hong;Jin Nam;Lee, Yong-Woo
    • Journal of Sericultural and Entomological Science
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    • v.41 no.2
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    • pp.108-115
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    • 1999
  • An improvement of methods in fibroin hydrolysates preparat significantly enlarges their applications to practical use. Acidic hydrolysis by hydrohloric acid is one of the methods for silk fibroin depolymeriza6tion. A low yield of final product and long time of the process are the demerits of this method. Possibility of preparation of water-soluble silk hydrolysates with more yield and less expenses is investigated in this study. Such possibility is occurred with the increasing tratment temperature and simultaneously decreasing treatment time, concentration of hydrochoric acid respectively, the concentration of sodium hydroxide used for neutraliza6tion of hydolysates after hydrolysis. Colour is decreased in this case and a small amount of activated, too. Protection of hydrolysates against precipitation after neutraliza6tion, and separation and during concentrating process is the other merit of this method. Creamy-coloured insoluble silk powder is the remainder of hydrolyzed fibroin. This is the only the immobiliza6tion of enzymes and other physiological active substances. Fine particles of this powder can be used as additives for artificial diets and cultural media, as well as raw materials for polymer membranes, etc.

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The genetic analysis on ancient human bone in jar coffin excavated from Naju Bokam-ni 3rd tumulus (출토 인골의 유전자분석-나주 복암리 3호분 옹관 인골을 중심으로)

  • Lee, Kyu-Sik;Chung, Yong-Jae;Han, Sung-Hee;Lee, Myong-Hee;Han, Myun-Soo;Choi, Dong-Ho
    • 보존과학연구
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    • s.20
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    • pp.5-20
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    • 1999
  • We have analyzed the allele and genotype frequencies from 10 fractions of ancient human skeleton in 3 pieces of Jar coffin excavated from Naju Bokamni3rd tumulus by PCR amplification, high resolution polyacrylamide gelelectorphoresis and silver staining. We could isolate human genomic DNA from 3 bone fractions but the rest of them could not be used as materials due to being decayed. We could detect sex determination as male and 3 genotypes of STR system, HUMTHO1, HUMTPOX and HUMC5F1PO from the bone fraction of left side in Jar coffin 3 and see the slightly reaction suggesting the sex as male from the bone fraction of the left side in Jar coffin 2 and female from the right side in Jar coffin 3.We have also analyzed the genotype frequencies of mitochondria from the bone fractions of the left side and the right side in jar coffin 3, respectively. From the result of indetifiying at nucletide position between 16018 and 16378of the base of hyper variable region(HV1) in the control region, We can presume that the both bones have the same maternal inheritance.

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Design of Fuzzy Model-based Multi-objective Controller and Its Application to MAGLEV ATO system (퍼지 모델 기반 다목적 제어기의 설계와 자기부상열차 자동운전시스템에의 적용)

  • 강동오;양세현;변증남
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 1998.10a
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    • pp.211-217
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    • 1998
  • Many practical control problems for the complex, uncertain or large-scale plants, need to simultaneously achieve a number of objectives, which may conflict or compete with each other. If the conventional optimization methods are applied to solve these control problems, the solution process may be time-consuming and the resulting solution would ofter lose its original meaning of optimality. Nevertheless, the human operators usually performs satisfactory results based on their qualitative and heuristic knowledge. In this paper, we investigate the control strategies of the human operators, and propose a fuzzy model-based multi-objective satisfactory controller. We also apply it to the automatic train operation(ATO) system for the magnetically levitated vehicles(MAGLEV). One of the human operator's strategies is to predict the control result in order to find the meaningful solution. In this paper, Takagi-Sugeno fuzzy model is used to simulated the prediction procedure. Another str tegy is to evaluate the multiple objectives with respect to their own standards. To realize this strategy, we propose the concept of a satisfactory solution and a satisfactory control scheme. The MAGLEV train is a typical example of the uncertain, complex and large-scale plants. Moreover, the ATO system has to satisfy multiple objectives, such as seed pattern tracking, stop gap accuracy, safety and riding comfort. In this paper, the speed pattern tracking controller and the automatic stop controller of the ATO system is designed based on the proposed control scheme. The effectiveness of the ATO system based on the proposed scheme is shown by the experiments with a rotary test bed and a real MAGLEV train.

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