• Title/Summary/Keyword: STR

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Studies on the Conditions of Cultivation and Drying of Lactic Acid Bacteria part I. Optimum Cultural Conditions of Lactic Acid Bacteria (유산균의 배양 및 건조조건에 관한 연구 제1보 유산균의 배양조건에 관한 연구)

  • Yoon, Joo-Chun;Kim, Kap-Soo;Shin, Won-Cheol;Lee, Keun-Eok;Choi, Seung-Rack;Yu, Ju-Hyun
    • Journal of Industrial Technology
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    • v.1
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    • pp.61-66
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    • 1981
  • In order to use as a starter after drying, optimum growth conditions of Str. thermophilus and L. bulgaricus were investigated. The results were as follows. The growth of Str. thermophilus was reached at stationary phase after 12 hours incubation and L. bulgaricus was after 6 hours. Str. thermophilus was grown well, in the medium containing 10% of skim milk, 2% of sucrose, 0.1% of KCl and 0.015% (v./v.) of soy-sauce. In the case of L. bulgaricus, 10% of skim milk, 2% of glucose, 0.5% of bacto-peptone, 0.1% of $K_2HPO_4$ and 0.02% of pyridoxine was suitable for the maximum growth.

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Investigation on antimicrobial resistance genes of Salmonella spp. isolated from pigs and cattle (소와 돼지유래 살모넬라속균의 약계내성유전자의 특성에 관한 연구)

  • Lee, Woo-Won;Jung, Byeong-Yeal;Lee, Gang-Rok;Lee, Dong-Soo;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.227-239
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    • 2009
  • At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM ($bla_{TEM}$) gene was detected from 51 (92.7%) of 55 $\beta$-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 $\beta$-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sulII (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

Effect of the Red Ginseng Extract on the Growth of Lactic Acid Bacteria (유산균(乳酸菌)의 발육(發育)에 미치는 홍삼(紅蔘) Extract의 영향(影響))

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.244-251
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    • 1984
  • In order to investigate the effect of red ginseng extract on the fermentation of lactic acid bacteria, 0.1-10.0% of red ginseng extract which chemical composition was 68.14% moisture, 10.11% crude protein, 2.66% crude fat, 5.23% crude ash, 56.90% total sugar, 18.80% reducing sugar and 9.09% crude saponin, respectively, were added to skim milk media. The effect of red ginseng extract on the fermentation and the growth rate of bacteria were tested. The results were as follow. 1. The acidity produced by Str. lactis and L. acidophilus for 24 hrs. were more increased by adding of red ginseng extract than control medium. But the acidity produced by L. helveticus was not significantly increased by adding of red ginseng extract. The fermentation time by Str. lactis and L. acidophilus were shortened by increasing the amount of red ginseng extract. 2. By increasing the amount of red ginseng extract, the acidity produced by lactic acid bacteria especially by Str. lactis and L. acidophilus were increased. 3. The number of bacteria were increased until 1.0% adding of red ginseng extract, but adding more than the above level, the effect was not clearly appeared.

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Analysis of Amelogenin Gene and Short Tandem Repeat(STR) loci LPL, F13B, F13A01, FESFPS, vWA from the Dental Calculus (치석에서 Amelogenin Gene 및 Short Tandem Repeat(STR) 유전좌위 LPL, F13B, Triplex(F13A01, FESFPS, vWA)에 대한 분석)

  • Kim, Sang-Bae;Choi, Jong-Hoon;Yoon, Chang-Lyuk;Kim, Chong-Youl
    • Journal of Oral Medicine and Pain
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    • v.24 no.2
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    • pp.219-234
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    • 1999
  • 치석에는 박리상피세포, 백혈구 등이 포함되어 있어 이들의 핵 내에 있는 DNA의 유전자형을 찾아내 개인식별을 할 수 있을 것으로 추정된다. 본 연구에서는 치석만으로 개인식별이 가능한지를 알아보고자 40명으로부터 채취한 치석을 증류수에 세척한 군과 세척하지 않은 군으로 나누어 DNA를 추출하고 중합효소연쇄반응법을 이용하여 증폭절편다형(AMP-FLPs)을 실시한 후 성별검사를 위한 X-Y homologous amelogenin gene과 유전자지문검사를 위한 STR유전좌위 LPL, F13B, Triplex(F13A01, FESFPS, vWA) 등 6개의 유전자를 검색하여 - X-Y homologous amelogenin gene과 LPL, F13B는 각각 증폭하였으며 F13A01, FESFPS, vWA 세 유전자는 동시에 증폭하였음 - 다음과 같은 결과를 얻었다. 1) X-Y homologous amelogenin gene 검색으로 세척군에서 27개의 검체 중 8개, 비세척군에서 13개 중 11개에서 성별검사가 가능하였다. 2) LPL유전자는 세척군, 비세척군에서 각각 27개 검체중 2개, 13개 검체 중 5개가 검색되었으며 3개의 대립유전자(10, 11, 12)와 4개의 유전자형 (10-10, 10-11, 10-12, 11-12)이 나타났다. 3) F13B유전자는 세척군, 비세척군에서 각각 27개 검체 중 1개, 13개 검체 중 4개가 검색되었으며 2개의 대립유전자(9, 10)와 2개의 유전자형(9-10, 10-10)을 관찰하였다. 4) F13A01유전자는 비세척군에서만 13개 검체 중 3개가 검색되었고 3개의 대립유전자(3.2, 4, 6)와 3개의 유전자형(3.2-3.2, 4-5, 4-6)을 관찰하였고, 세척군에서는 나타나지 않았다. 5) FESFPS유전자는 비세척군에서만 13개 검체 중 1개가 검색되었고 유전자 형은 11-12로 나타났다. 6) vWA유전자는 세척군, 비세척군에서 각각 1개씩 검색되었으며, 3개의 대립유전자형(14, 16, 17)와 2개의 유전자형(14-16, 14-17)을 관찰하였다. 이상의 결과를 종합해 볼 때, 치석은 X-Y homologous amelogenin gene증폭을 통한 성별검사와 단일 STR유전좌위 증폭을 통한 유전자지문형 검사에는 유용하나 복합 STR유전좌위의 검색에는 부적합한 것으로 나타났으며 법의학적시료로 응용이 가능한 것으로 사료된다.

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The Effects of Culture Conditions on the Diacetyl Production by Lactic Acid Bacteria (배양 조건에 따른 유산균의 Diacetyl 생성)

  • Kim, Dong-Wook;Chung, So-Young;Park, Ki-Moon;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.120-126
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    • 1989
  • Streptococcus diacetylactis and Leuconostoc cremoris were isolated from commercial culture and the effects of culture conditions on the diacetyl production by these strains and their mixture(1:1) were investigated. Optimum temperatures and culture times for the diacetyl production by Str. diacetylactis, Leu, cremoris and their mixture were 60hr at $22^{\circ}C$(diacetyl content, 2.24ppm), 48hr at $22^{\circ}C$(2.29ppm) and 48hr at $22^{\circ}C$ (2.21ppm), respectively Optimum initial pH for the diacetyl production by Str. diacetylactis, Leu. cremoris and the mixture were all 4.8(4.32, 6.66, 7.30ppm, respectively) and optimum sodium citrate concentrations(%, w/v) were 0.30(2.58ppm), 0.1(2.54ppm) and 0.1(2.52ppm), respectively. The diacetyl contents were gradually increased according as inoculation rates(%, w/v) were increased. The amounts of diacetyl produced under optimum conditions at 24hr incubation by Str. diacetylactis, Leu. cremoris and the mixture were 4.40, 6.59 and 7.25ppm, respectively. The most effective factor affecting diacetyl production under optimum condition was pH.

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A Study on the Fatigue Life Characteristics of Heat-Resisting Steel (내열강재의 마찰용접에 따른 피로수명 특성에 관한 연구)

  • 이동길;이상열;정재강
    • Proceedings of the KWS Conference
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    • 2000.10a
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    • pp.124-126
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    • 2000
  • The fatigue crack propagation characteristics were investigated for two kind of heat-resisting steel(STR3 and STR35) commonly using in valve material of vehicles. From the experiment, the fatigue life of the specimens notched at bonded line was shown about 19.7% and 72.2% lower and the specimens notched at 1.0mmdistance from bonded line was shown about 25.2% and 99.1% higher than that of the base metal respectably.

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Taxonomic Study on the Tribe Quediini(Coleoptera, Staphylinidae) from Korea (한국산 왕눈이반날개족 (딱정벌레목, 반날개과)의 분류학적 연구)

    • Animal Systematics, Evolution and Diversity
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    • v.12 no.2
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    • pp.107-119
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    • 1996
  • The tribe Quediini from Korea is examined. One new species, Velleius circumipectus, and one new subspecies, Quedius japonicus sachonensis, are described, and four species, Heterothops rotundiceps, Q. japonicus (s. str.), Q. simulans, and V. setosus, are recorded firstly in Korea. As a result, Korean fauna of the tribe Quediini comprises 10 species and one subspecies belonging to three genera.

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HARMONICS OF INDUSTRIAL POWER ELECTRONIC CONVERTERS IN THE EREQUENCY RANGE UP TO 10 KHZ

  • Buttner, J.;Krechla, A.;Petzoldt, J.;Machost, D.
    • Proceedings of the KIPE Conference
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    • 1998.10a
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    • pp.598-602
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    • 1998
  • This paper refers to fundamental investigations and simulations of conducted electromagnetic inference emissions produced by power electronic devices in a frequency range from 2 to 10 kHz. The emissions of different industrial power converters were measured and compared. The influence of different working conditions over the altitude of the EMI are represented. Simulations of the power converter system including the line impedance stabilisation network certify the measurements.

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Tension Control in Web Transport System using Direct Self-tuning Regulator (직접 STR을 이용한 웹 이송 시스템에서의 장력제어)

  • 오기석;권태종;한창수;강남기;조형진
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.11a
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    • pp.236-242
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    • 1996
  • The purpose of this paper is to study the tension control in a web transport system. Direct self-tuning regulator method was applied to tension controller and variable-gain PID control algorithm was applied to web speed controller. The designed controllers compensated for the time-varying parameters and tracked reference tension in process speed changing. The simulation shows that direct STR tension controller improves tension control performance in comparison with other controllers.

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제대혈을 활용한 유전자 캐릭터 제작 및 DNA 정보 데이터베이스 구축

  • Jeong, Gi-Hwa;Lee, Jong-In
    • Proceedings of the Korean Institute of Resources Recycling Conference
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    • 2003.10a
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    • pp.201-205
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    • 2003
  • 유전자 감식법은 1980년대 후반에 미국에서 처음으로 법의학적으로 적용된 이래, 강력범죄, 친자확인 및 항공기 추락사고, 화재 등의 대형 참사의 신원 확인에 큰 공헌을 하였다. 유전자감식을 위해서는 높은 다형성을 보이는 STR (short tandem repeats) 좌위의 타이핑을 주로 이용한다. 한편, 최근 줄기세포 (stem cell)의 중요성이 인식되면서 폐기되던 제대혈의 보관 사업이 활성화되고 있다. 본 연구에서는 제대혈의 보관시 STR 타이핑의 유전 정보를 표기한 유전자 캐릭터를 동시에 제공하여 불의의 사고시 신원확인이 가능하게 하고, 아울러 이산가족에 대한 DNA정보 데이터베이스 구축 및 검색 프로그램을 개발하였다.

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