• BMB Reports
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• v.47 no.11
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• pp.643-648
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• 2014

Stanniocalcin (STC), a glycoprotein hormone originally discovered in fish, has been implicated in calcium and phosphate homeostasis. While fishes and mammals possess two STC homologs (STC1 and STC2), the physiological roles of STC2 are largely unknown compared with those of STC1. In this study, we identified Ran-binding protein M (RanBPM) as a novel binding partner of STC2 using yeast two-hybrid screening. The interaction between STC2 and RanBPM was confirmed in mammalian cells by immunoprecipitation. STC2 enhanced the RanBPM-mediated transactivation of liganded androgen receptor (AR), but not thyroid receptor ${\beta}$, glucocorticoid receptor, or estrogen receptor ${\beta}$. We also found that AR interacted with RanBPM in both the absence and presence of testosterone (T). Furthermore, we discovered that STC2 recruits RanBPM/AR complex in T-dependent manner. Taken together, our findings suggest that STC2 is a novel RanBPM-interacting protein that promotes AR transactivation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.307-313
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• 2010

Stanniocalcin 1 (STC1) is a glycoprotein hormone that is important in the maintenance of calcium and phosphate homeostasis in both fish and mammals. STC1 and its paralog STC2 are expressed in multiple tissues in fishes, although the physiological roles of piscine STCs are still unclear compared with those of mammals. In this study, we cloned olive flounder STC1 (ofSTC1) and ofSTC2 cDNAs into pET28a vector and used E. coli Rosetta (DE3) as the host strain for protein expression. Expression experiments were carried out using isopropyl-$\beta$-D-thiogalactoside (IPTG) and nickel affinity chromatography. We could identify the recombinant proteins as single 29.5 kDa (ofSTC1) and 33.2 kDa (ofSTC2) bands in the insoluble fraction on sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). These results indicate that ofSTC1 and ofSTC2 were expressed as insoluble proteins in E. coli. Furthermore, the injection of ofSTC1 protein into juvenile tilapia resulted in a decrease of the serum calcium level. These results suggest that the purified fish STC1 and STC2 proteins may be used to elucidate the physiological role of STCs in fishes.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.273.2-273.2
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• 2001

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.246.2-246.2
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• 2001

• BMB Reports
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• v.45 no.11
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• pp.629-634
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• 2012

The human glycoprotein, stanniocalcin 2 (STC2) plays multiple roles in several tumor types, however, its function and clinical significance in hepatocellular carcinoma (HCC) remain unclear. In this study, we detected STC2 expression by quantitative real-time PCR and found STC2 was upregulated in HCC tissues, correlated with tumor size and multiplicity of HCC. Ectopic expression of STC2 markedly promoted HCC cell proliferation and colony formation, while silencing of endogenous STC2 resulted in a reduced cell growth by cell cycle delay in G0/G1 phase. Western blot analysis demonstrated that STC2 could regulate the expression of cyclin D1 and activate extracellular signal-regulated kinase 1/2 (ERK1/2) in a dominant-positive manner. Transwell chamber assay also indicated altered patterns of STC2 expression had an important effect on cell migration. Our findings suggest that STC2 functions as a potential oncoprotein in the development and progression of HCC as well as a promising molecular target for HCC therapy.

• Journal of Radiation Protection and Research
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• v.40 no.4
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• pp.244-251
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• 2015

The containment filtered venting system reduces the range of the contamination area around the nuclear power plant by strengthening the integrity of the containment building. In this study, the probabilistic assessment code MACCS2 was used to assess the effect of the CFVS to off-site. The accident source term was selected from a Probabilistic Safety Analysis report of SHINKORI 1&2 Nuclear Power Plant. The three source term categories from 19 STC were chosen to evaluate the effective dose and thyroid dose of residents around the power plant and the dose with CFVS and without CFVS were compared. The dose was calculated according to the distance from the nuclear power plant, so the damage scale based on the distance that exceeds the IAEA criteria for effective dose (100 mSv per 7 days) and thyroid dose (50 mSv per 7 days) were compared. The effective dose reduction rates of the STC-3, STC-4, STC-6 were about 95-99% in the whole range (0~35 km), 96-98% for the thyroid dose. There are similar results between effective dose and thyroid dose. After applying the CFVS, the damage scale that exceeds the effective dose criteria was about 1 km (mean). Especially, the STC-4 damage scale was decreased from 26 km (mean) to 1.2 km (mean) significantly. The damage scale that exceed the thyroid dose criteria was decreased to 2~3 km (mean). The STC-4 damage scale was also decreased significantly as compared to STC-3, STC-6 in terms of effective dose.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.233.2-234
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• 2001

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.217-224
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• 2014

A total of 150 growing ducks were assigned to five dietary treatments to study the effect of sea tangle and charcoal (STC) supplementation on growth performance and meat characteristics in a completely randomized design. There were six replicates and five ducklings in each replication. The five dietary treatments were control, antibiotic, and 0.1%, 0.5%, and 1% STC supplemented diets. No significant differences were found on ADG, ADFI, and gain:feed among treatments in different weeks. The overall (0 to 3 weeks) ADFI decreased in antibiotic treatment (p<0.05) whereas the gain:feed increased significantly upon 1.0% STC supplementation compared to control (p<0.05). No significant variation was found in meat chemical composition except crude fat content which was high in 1.0% STC dietary group (p<0.05). Meat cholesterol was reduced in 0.1% STC group (p<0.05) compared to other dose levels while serum cholesterol was unaffected. High density lipoprotein (HDL) content was high in 1.0% STC (p<0.05) and low density lipoprotein (LDL) was low in 0.1% and 1.0% STC dietary groups (p = 0.06). No significant effect was found on the thiobarbituric acid reactive substances (TBARS) of fresh meat, whereas the TBARS value of meat preserved for 1 week was reduced significantly in STC dietary groups (p<0.05). The 0.1% STC dietary group showed an increased myristic acid (p = 0.07) content whereas, the content of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids increased in STC supplementation than antibiotic group (p<0.05). An increased concentration of omega-3 fatty acids and a reduced ratio of n-6/n-3 PUFA ratio was found upon 1.0% STC supplementation compared to antibiotic dietary group (p<0.05). Therefore, 1.0% STC dietary supplementation can be used as alternatives to antibiotics in duck production.

• The KIPS Transactions:PartA
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• v.8A no.2
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• pp.157-166
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• 2001

A simulation package has been developed as an event-driven system that can handle the hardware configuration of STC104 and algorithm proposed in the sister paper of ‘Study on High Speed Routers(II).’After various STC104 topology of meshes, torus, and hypercubes are constructed using up to 512 switches, the performance of each topology has been analyzed under different message generation rate in terms of throughputs, latency, and packet blocking time. Modified multicast algorithms for STC104 have been proposed for STC104 after U-mesh and U-torus in order to overcome the multicasting difficulty because of the point-to-point communication method found in STC104. The performance of the multicast algorithms have been analyzed over meshes and torus configuration. Throughput gets higher in the order of mesh, torus, and hypercube. Throughput difference among topology were distinctive in the zone of high message generation rate. Latency and blocking time increased in the order of hypercube, torus, and mesh. U-mesh and U-torus of software multicast showed similar throughput, however, U-mesh peformed slightly better result. These algorithms showed eight to ten times better results compared to individual message pass for 90 destination nodes. Multi-link environment also showed better performance than single-link environment because multi-link network used the extra links for communication.

• Journal of Satellite, Information and Communications
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• v.3 no.2
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• pp.1-6
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• 2008

Space-time codes (STC) can achieve the diversity gain in a multi-path environment without additional bandwidth requirement. Recent study results reported that satellite systems can also achieve this diversity gain by using space-time codes in a cooperative network with terrestrial repeaters. Due to uni-directional nature of satellite DMB services, transmit diversity can be considered as one of the most effective ways to improve the performance. In this paper, we demonstrate various simulations results of STC schemes which can contribute to improve performance of future satellite DMB services. The STC schemes introduced in this paper provides diversity gains by combing independent coded signal from the satellite as well as from the terrestrial repeaters. In addition, we discuss a few points which should be considered to develop and implement these STC schemes.