• Title/Summary/Keyword: STA1

Search Result 186, Processing Time 0.032 seconds

Simultaneous Detection of Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus by Multiplex Polymerase Chain Reaction (Multiplex Polymerase Chain Reaction(PCR)법을 이용한 Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus의 다중동시검출)

  • Jeong, Yoo-Seok;Jung, Hee-Kyoung;Jeon, Won-Bae;Seo, Hwa-Jung;Hong, Joo-Heon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.39 no.4
    • /
    • pp.595-601
    • /
    • 2010
  • This study was conducted to detect and identify Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella enterica subsp. using simultaneous multiplex polymerase chain reaction (multiplex PCR) assay. 23S rRNA partial gene (S. aureus), tox R gene (V. parahaemolyticus), and inv A gene (S. enterica subsp.) as diagnostic marker gene were suggested, and their amplicon sizes were 482 bp, 368 bp, and 284 bp, respectively. Non specific amplicons by STA-5F/STA-5R primer, ToxR-F/ToxR-R primer, and 139/141 primer were not observed in genomic DNA of pathogen bacteria as Bacillus cereus, Listeria monocytogenes, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, Candida albicans, and Shigella sonnei. The extracted crude DNA of targeted bacteria was detected as PCR template successfully. The detection limits were $10^5\sim10^4$ CFU/mL and 10 pg of purified genomic DNA of S. aureus, V. parahaemolyticus, and S. enterica subsp. by using simultaneous multiplex PCR.

Influence of Different Phosphorus Fertilizer to Barley Growth and Yield (맥류에 대한 각종 인산질 비료의 비효검정 시험)

  • Cho, C.H.;Ha, Y.W.;Hong, B.H.;Kim, D.K.;Huh, W.S.;Lee, J.S.;Kang, J.C.;Chai, J.S.;Lee, D.K.;Park, K.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.13
    • /
    • pp.119-126
    • /
    • 1973
  • In order to investigate the effects of phosphorous fertilizer such as fused Phosphate, compound fertilizer and triple phosphate on barley(growth and yield at the fixed level of nitrogen and potassium, 4 level of hosphorous) has been tested at Suwon. Iri. Kwangju ani Daegu with randomized completeblock design. The results obtained could be summarized as follows. 1. In four locations. increased application of phosphorus have brought earlier maturity in barley regardless the type of fertilizer but fused phosphate considered to have more effects on stimulation of maturity of barley compare with the others. 3. Fused phosphate increased yield higher by-8kg/10a application compare with the same level of compound fertilizer and triple phosphate but there was no differences in yield at 12kg/10a phosphate but there was no differences in yield at 12kg/l0a application among the fertilizers except Kwangju and Suwon. 3. Grain weight was considerably higher in application of fused phosphate Thus are considered effects phosphate. 4. Optimum amount of phosphorous fertilizer was considered 8kg/l0a. Application of 4kg per 10a produced lower yield than 8kg and slightly or no increased yield was observed in 12kg application. 5. Among the 4kg/10a applied condition of phosphorous fertilizers, yield was decreased at fused phosphate treatment compare with the others and this fact resulted supposedly due to the lower portion of valid phosphorus in fused phosphate because of its citric acid solubility. At level of 12kg/10a application fused phosphate was considered more effective in yield increment.

  • PDF

Catastrophic Health Expenditure and Trend of South Korea in 2018 (2018년 재난적 의료비 경험률 현황 및 추이)

  • Jeong, Wonjeong;Kim, Yunkyung;Park, Eun-Cheol
    • Health Policy and Management
    • /
    • v.30 no.1
    • /
    • pp.126-130
    • /
    • 2020
  • Catastrophic health expenditure refers to measure the level of the economic burden of households due to medical expenses. The purpose of this study was to examine the proportion of households that experienced catastrophic health expenditure between 2006 and 2018 using available data from the National Survey of Tax and Benefit (NaSTaB), Korea Health Panel (KHP), and Households Income and Expenditure Survey (HIES). Trend test was used to analyze the proportion of household with catastrophic healthcare expenditure. The households experienced the catastrophic health expenditure 2.08% in 2018 using the NaSTaB data. Trend analysis was significant with the decreasing trend (Annual Percentage Change [APC], -4.88; p<0.0001) in the proportion of households with the catastrophic health expenditure. On the other hand, the results of the HIES showed 2.92%, and KHP showed 2.48% of households experienced the catastrophic health expenditure in 2016. The trend was significantly increased in HIES (APC, 1.43; p<0.0001) and KHP (APC, 6.68; p<0.0001). Therefore, this suggests that further interventions to alleviate the burden of catastrophic health expenditure to the low-income group are needed.

Development of the Brown Planthopper, Nilaparvata lungens Sta$\{aa}$l, Biotypes and Their Hybrid Progenies Fed on Different Cultivars of Rice with Various Resistance Gene and Damage of Different Rice Cultivars (벼멸구 생태형의 교잡종별 발육 및 수도 품종별 피해)

  • 최주수;박영도
    • Journal of Life Science
    • /
    • v.7 no.4
    • /
    • pp.347-354
    • /
    • 1997
  • Some attempts were made to investigate the gentic characteristics of the brown planthopper(BPH), Nilaparvata lugens Sta${\aa}$l, biotypes. In terms of egg and nymphal periods, egg hatcgability, emergence ratio, and population change of BPH and plant damage of rice cultivars by the infestation of BPH biotypes and their hybrid progenies in field conditions. The egg and nymphal periods of the three of BPH biotypes and their hybrid progenies on Dongjinbyeo with no resistance gene were shorter than on rice cultivars with resistance gene. But the periods of biotype-2(♀) X 2(♂) (E) on Samgangbyeo with Bph-1 gene and biotype-3(♀) X 3(♂)(I) on Mirying 63 with bph-2 gene were as short as on Dongjinbyeo. The egg hatchability and emergence ratio of the three Bph biotypes and their hybrid progenies on Dongjinbyeo were higher than on the other rice cultivars, and those of E on Samgangbyeo and I on Miryang 63 were as high as on Dongjinbyeo. In rice paddy field, biotype-1(♀) X 1(♂)(A)$\sim$I infested on Dongjinbyeo, E onSamgangbyeo, and I on Miryang 63 were increased their population densities remarkably in next generation. Dongjinbyeo infested by A$\sim$I, and Myryang 63 infested by I were hopperburned at 60 days after infestation and had a damage rating of 0.9. But Samgangbyeo infested by E was no hopperburn(damage rating: 7.3) though it showed high BPH population at 60 days after infestation.

  • PDF

Effects of Applying Slow-Release Fertilizer on Southern Type Garlic (Allium sativum L.) Cultivation (난지형 마늘 재배 시 완효성 비료 시용효과)

  • Song, Yeon Sang;Choi, In Hu;Chung, Byeong Choon;Choi, Won yul
    • Horticultural Science & Technology
    • /
    • v.19 no.4
    • /
    • pp.471-475
    • /
    • 2001
  • This experiment was conducted to evaluate the effect of slow-release fertilizer on warm type garlic cultivation in the Southwestern region of Korea. The slow-release fertilizers used were UF(Urea-Formaldehydes)70, 100, 130, CDU(Crotonylidene Diurea)100, and IBDU(Isobuty- lidene Diurea)100. The slow-release fertilizer treatment slightly increased plant height, leaf sheath diameter, and leaf number compare with conventional fertilizer treatment. Also, large bulb rate, nitrogen and pyruvic acid contents of garlic in slow-release fertilizer plot were higher than other treatments. Application of slow-release fertilizer increased the commercial yields.

  • PDF

A Study on Newborn Infection during Hospitalization (일 종합병원 신생아실 감염증에 관한 소고)

  • Lee Jung Hee;Jung Moon-Hee;Kim Sung Rae
    • Journal of Korean Public Health Nursing
    • /
    • v.5 no.2
    • /
    • pp.67-75
    • /
    • 1991
  • This study was conducted to evaluate the difference of staphylococcal colonization between lactating mothers and nursery stares. Samples were obtained from the hands and noses of the newborn(admitted to S Univ. hospital's nursery from 1991. 5. 23 to 6. 8), their mothers and nursery staffs. They were cultured, and then the presence/absence of pathogenic staphylococci in them were analyzed by Fisher exact probability test which led to these conclusion of significance as follows: 1. No differences were detected in pathogenic rate of nasal swab culture between breast~fed newborns and formula-fed ones. 2. Delivery type and hospitalization the ones from the brest-fed newborns show high rate of Sta. aureus coa(+). In case of either C-sec delivery or long (more than 4 days) hospitalization, formula-fed ones in case of NSVD or short (less than 3 days) hospitalization. 3. The ones from breast feeding mothers show higher sta. aureus coa(+) than those from nursery staffs, which was of no significance statistically. Considering nursery staffs only, however, the comparison of those with the data in April indicates that the pathogen rate is higher for hand than nose, and Sta aureus coa(+) for hand is far lower in June than in April, which was statistically significant. These results lead us to infer that newborn infection in hospitalization could be largely reduced by maintaining the ordinary hygienes. Such as the handwashing of mothers as well as nursery staffs (directly involved in newborn care) Thus the pre-enterance hand washing of a mother who visits the nursery only for breast feeding (without any other medical responsibility) should be done, like other medical agents, with $0.05\%$ chlorhexidine antiseptics rather than simple soap cleansing; the one 'that is worth emphasizing thoroughly.

  • PDF

Effective Screening Condition for Low-Temperature Germinability of Rice (벼 저온발아성의 효율적 검정 조건)

  • Kang, Jong-Re;Lim, Sang-Jong;Kim, Soon-Chul;Ko, Mi-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.40 no.6
    • /
    • pp.711-715
    • /
    • 1995
  • This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

  • PDF

Development of Reactive Blue Dyestuffs with Bi-functional Reactivity (이종이관능형(異種二官能形) 반응성 Blue 염료의 개발)

  • Kim, Woon-Tai;Park, Jae-Young;Kim, Byoung-Moo;Nam, Won-Woo;Sohn, Byoung-Chung
    • Journal of the Korean Applied Science and Technology
    • /
    • v.13 no.2
    • /
    • pp.21-28
    • /
    • 1996
  • Diazotization of STA synthesized by the indirect diazotization method. The effect of catalyst and agitation (rpm) about STA and H-acid reaction were examined and the optimum conditions were investigated experimentally. The yields and characteristics of $1^{.st}$ coupling and diazotization synthesis were identified by HPLC and FT-IR analysis. $1^{.st}$ condensation of CNC and mPDSA were synthesized at $5^{\circ}C$ and pH=6.5. The conditions of alkaly coupling of H-acid were synthesized at $5^{\circ}C$ and pH=8. The condensation of products was identified by U. V. analysis. From this results, It was obtained to reactive dyestuffs of bi-functional reactivity with high fastness and high adsorption.

The Slope Stability Establishment with P.C. Anchoring Method (P.C. Anchoring 공법을 활용한 사면안정대책)

  • 박철숙;손재호;인영길;장두희
    • Proceedings of the KSEG Conference
    • /
    • 2002.04a
    • /
    • pp.61-73
    • /
    • 2002
  • Daegok dam is Concrete Face Rockfill Dam(C.F.R.D) of which 52m height, 190m length, that construction in beneath 306, Chanjeon-Ri, Dudong-Myeon, Ulju-Gun, Ulsan Metrocity. Left slope excavation of spillway have related to Daegok dam construction are developing crack in Sta. No. 1~2, EL. 134~137m after 67.0mm rainfall from 2000. 7. 23. 13:00 to 7. 24. 04:00. Surface geological survey and slope stability investigation with stereographic projection method in order of slope stability establishment. Partial supplement excavation and SSL. P. C. Anchoring method is able to pre-stressing are think about unstable element after excavation. This slope stability establishment is very successfully completion.

  • PDF

Microvascular Anastomosis in Neurosurgical Field (신경외과 영역에서의 미세혈관 문합술)

  • Rha, Hyoung-Kyun;Lee, Kyung-Jin;Cho, Kyung-Keun;Park, Sung-Chan;Park, Hae-Kwan;Cho, Jung-Ki;Ji, Chul;Choi, Chang-Rak
    • Archives of Reconstructive Microsurgery
    • /
    • v.8 no.2
    • /
    • pp.170-175
    • /
    • 1999
  • Objective : Extracranial-intracranial(EC-IC) microvascular anastomosis was performed in 18 patients with hemodynamic cerebral ischemia and traumatic cerebral aneurysm, the aim of this retrospective study was to assess its value in neurosurgical field. Method : Of 18 cases, 17 case were hemodynamic cerebral ischemia and one was traumatic cerebral aneurysm. There were 14 superficial temporal artery(STA)-to-middle cerebral artery(MCA) anastomosis, 3 saphenous vein graft bypass(2 external carotid artery(ECA)-to-MCA, 1main trunk of the STA-to-MCA) and 1 radial artery bypass(ECA-to-MCA). Results : Bypass patency was confirmed by postoperative angiography in all cases except for two cases, postoperative cerebral blood flow of ischemic brain showed significant increased in all cases with good patency through bypass. Conclusion : Revascularization by EC-IC microvascular anastomosis to the ischemic brain eliminated ischemia and was associated with excellent good outcome and good patency rates.

  • PDF