• Journal of Animal Science and Technology
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• 제62권4호
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• pp.543-552
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• 2020

For efficient prevention and treatment of enteric colibacillosis, understanding about latest virulence factors and antimicrobial resistance of Escherichia coli is essentially needed. The aim of this study was to survey antimicrobial resistance and determine the prevalence of fimbriae and enterotoxin genes among 118 pathogenic E. coli isolates obtained from Korean pigs with diarrhea between 2016 and 2017. The genes for the toxins and adhesins were amplified by polymerase chain reaction (PCR). The susceptibility of the E. coli isolates to antimicrobials were tested using the standard Kirby-Bauer disk diffusion method. The most prevalent fimbrial antigen was F18 (40.7%), followed by F4 (16.9%), and the most prevalent combinations of toxin genes were Stx2e (21.2%), STb:EAST-1 (19.5%), and STa:STb (16.9%), respectively. Among the pathotypes, enterotoxigenic E. coli (ETEC) was the most predominant (67.8%), followed by Shiga-toxin producing E. coli (STEC, 23.7%). We confirmed high resistance rates to chloramphenicol (88.1%), tetracycline (86.4%), streptomycin (86.4%), and ampicillin (86.4%). And the majorities of isolates (90.7%) showed multi-drug resistance which means having resistance to 3 or more subclasses of antimicrobials. Results of this study can be a source of valuable data for investigating the epidemiology of and control measures for enteric colibacillosis in Korean piggeries.

• 한국미생물·생명공학회지
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• 제20권6호
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• pp.699-703
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• 1992

본 연구에서는 간염치료제로 효과가 있는 민간제제(NS-P)의 성분 분석 및 항균 활성과 항산화 활성을 연구하였다. 이 NP-S는 그람 양성균인 Staphylococcus aureus와 그람 음성균인 Salmonella typhimurium 그리고 진균인 Candida albcans에 항균 활성을 보여 주었을 뿐만 아니라, 지질의 과산화를 억제하는 항산화 활성을 나타냈다. 이러한 NP-S는 C/H/N/S의 원소비가 15.5/4.8/11/10으로 황(S)이 상당히 많이 존재하고 있으며, 휘발성 물질인 암모니아를 2.11M 함유하고 있었다. 이 외에도 6.7의 펩타이드, 0.3의 유리 아미노산, 0.1의 무기 인산염을 함유하고 있으며, 미량 원소로서 Cl, Si, Mg, Zn도 함유하고 있다.

• Computers and Concrete
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• 제16권1호
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• pp.141-161
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• 2015

A significant portion of residential areas of Turkey is located in active earthquake zones. In Turkey occurred major earthquakes in last twenty years, such as Erzincan (1992), Kocaeli and $D{\ddot{u}}zce$ (1999), $Bing{\ddot{o}}l$ (2003), Van (2011). These earthquakes have demonstrated that reinforced concrete (RC) buildings having horizontal and vertical irregularities are significantly damaged, which in turn most of them are collapsed. Architectural design and arrangement of load-bearing system have important effect on RC building since architectural design criteria in design process provide opportunity to make this type of buildings safer and economical under earthquake loads. This study aims to investigate comparatively the effects of weak story irregularity on earthquake behavior and rough construction costs of RC buildings by considering different soil-conditions given in the Turkish Earthquake Code. With this aim, Sta4-CAD program based on matrix displacement method is utilized. Considering that different story height and compressive strength of concrete, and infill walls or their locations are the variables, a set of structural models are developed to determine the effect of them on earthquake behavior and rough construction costs of RC buildings. In conclusion, some recommendations and results related to making RC buildings safer and more economical are presented by comparing results obtained from structural analyses.

• Journal of Korean Neurosurgical Society
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• 제57권1호
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• pp.54-57
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• 2015

A 66-year-old woman presented with intermittent paraparesis and generalized tonic-clonic seizure. Cerebral angiography demonstrated dural arteriovenous fistula (AVF) involving superior sagittal sinus (SSS), which was associated with SSS occlusion on the posterior one third. The dural AVF was fed by bilateral middle meningeal arteries (MMAs), superficial temporal arteries (STAs) and occipital arteries with marked retrograde cortical venous reflux. Transfemoral arterial Onyx embolization was performed through right MMA and STA, but it was not successful, which resulted in partial obliteration of dural AVF because of tortuous MMA preventing the microcatheter from reaching the fistula closely enough. Second procedure was performed through left MMA accessed by direct MMA puncture following small decortications of cranium overlying the MMA using diamond drill one week later. Microcatheter could be located far distally to the fistula through 5 F sheath placed into the MMA and complete obliteration of dural AVF was achieved using 3.9 cc of Onyx.

• 한국미생물·생명공학회지
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• 제17권2호
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• pp.160-164
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• 1989

알카리성 Bacillus sp. AL-8의 알카리성 amylase 유전자를 포함하는 5.7Kb의 EcoRI 단편을 pUB 110을 vector로 하여 amylase를 생산하지 못하는 B. subtilis sta-1에서 발현시켰다. 재조합 plasmid pMB802와 pMB809는 숙주세포인 B. subtilis에서 매우 안정하게 유지되었으며 amylase 생산이 공여균 주에서 보다 1.8배 증가하였다. 형질전환주에서 생산된 amylase는 공여균주와 같은 효소적 성질을 나타내었다. 5.7Kb 단편을 E. coli에 subcloning한 결과 3.7Kb의 EcoRI 단편에 알카리성 amylase 유전자가 존재하였다.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.69-70
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• 2003

정원세포는 수컷의 정소에 존재하며, 감수분열을 통하여 정자를 형성하고 지속적으로 자신의 복제가 가능한 세포이다. 이러한 정원세포는 마우스를 중심으로 현재 수컷불임 치료의 연구, 멸종위기 종의 보존을 위한 연구 그리고 형질전환 동물 생산 등 다양한 분야에 응용되고 있다. 그러나 조류에서는 정원세포 연구에 있어서 그 세포의 형태적·면역학적 특성이 아직 구명되지 못하여 연구진행에 어려움이 많다. 따라서 본 연구에서는 전자주사현미경을 이용하여 세포내 구조와 형태의 분석을 진행하였고, 조직염색법을 통하여 특이적 마커를 분석하였다. 이후 본 연구결과를 토대로, 포유류에서 이루어진 정원세포이식기술의 개발 및 새로운 형질전환 기술의 개발을 조류응용이 가능할 것이다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.128.3-129
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• 2003

A leaf blight disease caused by a species of Phytophthora has been observed on castor bean plants growing near dwelling houses in Manchon-dong, Daegu since 1993. The first isolate that we have kept was producing papillate, ovoid-obpyriform to obpyriform sporangia with on a simple sympodial sporangiophore from diseased tissue placed on water agar plates. The pure isolate, however, did not sporulate on agar media, and rarely even in water, but produced mycelial swellings and chlamydospores in water. Sporangia measured 26.1-77.4 ${\times}$ 23.2-44.0$\mu\textrm{m}$. Chlamydospores were either terminal or intercalary, and measured 24-29.4$\mu\textrm{m}$ in diameter. Sex organs were not formed in a single culture. In 2003, another pure isolate was isolated from castor bean plants with similar symptoms at the same place. The second isolate was distinct from the first one in that the second isolate was readily and abundantly sporulating on V8 juice agar plates. Sporangia of the second isolate were papillate, ovoid and caduceus with a pedicel. Sporangia measured 19.5-48.8 x 17.6-34.3$\mu\textrm{m}$ with 3.7$\mu\textrm{m}$ high papilla and 4.1$\mu\textrm{m}$ long pedicel. No sex organs were formed in a single isolate culture. Both isolates were pathogenic on castor bean plants. Results of the efforts to identify the two species of Phytophthora will be discussed.

• 한국운동역학회지
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• 제26권3호
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• pp.315-321
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• 2016

Objective: The aim of this study was to analyze effects of the toe-spring angle of bobsleigh shoes on start speed lap time to develop Korean-specific bobsled shoes suitable for winter environments and for domestic players on the basis of sports science and optimized biomechanical performance. Method: Seven Korean bobsleigh athletes participated in this study, with three pairs of sprint spikes from three companies (Type A, Type B, Type C). To analyze sprint lap time and forefoot bending angle for each shoe, participants were instructed to drag a sled 15 meters from the start line at a maximum sprint. forefoot bending angle was collected by a high speed camera, and lap time speed was measured. Results: Lap time for type B shoes was $3.52{\pm}0.17sec$, type A was $3.55{\pm}0.19sec$, and type C was $3.56{\pm}0.18sec$. Forefoot bending angles were: angle 1, $6.88{\pm}5.55^{\circ}$; angle 2, $9.23{\pm}6.38^{\circ}$; angle 3, $15.56{\pm}5.39^{\circ}$; angle 4, $9.54{\pm}3.85^{\circ}$; angle 5, $9.22{\pm}5.08^{\circ}$; angle 6, $7.66{\pm}6.44^{\circ}$; and angle 7, $4.30{\pm}6.24^{\circ}$ (p<.001). Forefoot bending in angle 3 was as follows: type A, $16.47{\pm}6.01^{\circ}$; type B, $14.30{\pm}4.96^{\circ}$; and type C, $15.90{\pm}5.17^{\circ}$. Conclusion: Hard outsoles and midsoles are better than soft type for reduced start lap time when developing a prototype Korean bobsled shoe.

• Bulletin of the Korean Chemical Society
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• 제21권10호
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• pp.969-972
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• 2000

[Fe II Fe III $BPLNP(OAc)_2](BPh_4)_2$ (1), a new model for the reduced form of the purple acid phosphatases, has been synthesized by using a dinucleating ligand, 2,6-bis[((2-pyridylmethyl)(6-methyl-2-pyridylmethyl)ami-no)methyl]-4-nitrophenol (HBPLNP) . Complex 1 has been studied by electronic spectral, NMR, EPR, SQUID, and electrochemical methods. Complex 1 exhibits two strong bands at 498 nm $(\varepsilon=$ 2.6 ${\times}10^3M-^1cm-^1)$ and 1363 nm $(\varepsilon=$ 5.7 ${\times}10^2M-^1cm-^1)$ in $CH_3CN.$ These are assigned to phenolate-to-FeIII and intervalence charge-transfer transitions, respectively. NMR spectrum of complex 1 exhibits sharp isotropically shifted resonances, which number is half of those expected for a valence-trapped species, indicating that electron transfer between FeⅡ and FeⅢ centers is faster than NMR time scale at room temperature. Complex 1 undergoes quasireversible one-electron redox processes. The $FeIII_2/FeIIFeIII$ and $FeIIFeIII/FeII_2$ redox couples are at 0.807 and 0.167 V ver-sus SCE, respectively. It has Kcomp = 5.9 ${\times}$10 1s(acetato) ligand combination sta-bilizes a mixed-valence FeIIFeIII complex in the air. Interestingly, complex 1 exhibits intense EPR signals at g = 8.56, 5.45, 4.30 corresponding to mononuclear high-spin FeⅢ species, which suggest a very weak magnetic coupling between the iron centers. Magnetic susceptibility study shows that there is a very weak antiferromag-netic coupling (J = $-0.78cm-^1$, H = $-2JS_1${\times}$S_2)$ between FeII and FeIII centers. Thus, we can suggest that complex 1 has a very weak antiferromagnetic coupling between the iron centers due to the electronic effect of the nitro group in the bridging phenolate ligand.

• 한국초지조사료학회지
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• 제16권1호
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• pp.17-26
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• 1996

This experiment was carried out to investigate the effects of endophyte staWendophyte-free and -infect) and cutting frequency(3, 4, 5 and 6 timeslyear) on the forage quality of tall fescue(Festuca crnrtzdit~crcerr Schreb.) at the Experimental Field of Grassland and Forage Crops Division. National Livestock Research Institute, Suweon, from 1993 to 1994. 'Ihe results obtained are summarized as follows: CP content of tall fexue was increased with endophyte-free and cutting frequency for two varieties. Crude protein yield(CPY) of tall fescue in 1993 was increased with endophyte-free and cutting frequency for two varieties(P<0.05). In 1994, however. CPY of endophyte-free tall fescue was slightly lower than that of endophyte-infected tall fescue and the higher CPY was observed when tall fescue was harvested 6 times per year. NDF content of tall fescue was not different between endophyte-infected and -free. NDF was decreased with cutting frequency for two varieties in 1993, however. it was not influenced by the endophyte and cutting frequency in 1994. ADF content of tall fescue was not different between endophyte-infected and -free. and it was decreased with cutting frequency for two varieties. IVDMD was not influenced by endophyte, however, it was increased with cutting frequency for two varieties. Based on the results of this experiment, it is suggested that a slight increase in forage quality could be obtained from endophyte-free tall fescue and the quality was increased with frequent cutting.