• Korean Journal of Acupuncture
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• 제20권3호
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• pp.115-128
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• 2003

Objectives and methods :　Study the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Evodiae Fructus infusion solution, we injected Evodiae Fructus infusion solution into Zusanli(St36) of C57BL/6 mouse which is corresponding to human Zusanli(St36). We observed its effect on the number of $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in mouse PBMCs, the number of the pulmonary colony , MST and ILS of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results and Conclusions : The spleen cells proliferation of the sample groups treated with EDR-HAS extract has increased significantly compared with that of the control group. The percentage of the $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in C57BL/6 mouse PBMCs of the sample groups treated with EDR herbal-acupuncture has increased compared with that of the control group. The lung colony number of the sample groups treated with EDR herbal-acupuncture has decreased significantly compared with that of the control group. MST and ILS of the sample groups treated with EDR herbal-acupuncture have increased significantly compared with those of the control group.

• Journal of Acupuncture Research
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• 제38권1호
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• pp.47-59
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• 2021

Background: This study aimed to assess the toxicity of Aconitum sinomontanum Nakai (ASN) pharmacopuncture. Methods: To investigate the toxicity of ASN pharmacopuncture, single and 4-week repeated dose toxicity experiments were conducted on BALB/c mice. In the single-dose toxicity experiment, mice were assigned 1 of 4 groups (5 males, 5 females per group). Then, 31.25, 62.5, and 125 mg/kg of ASN pharmacopuncture were administered to the mice in the experimental groups at acupoint ST36, while 0.2 mL of normal saline was administered to the control group at ST36. After a 4-week repeated dose regimen, the mice were assigned into 4 groups (5 males, 5 females per group). Then, 15.625, 31.25, and 62.5 mg/kg of ASN pharmacopuncture at ST36 were administered to the mice in the experimental groups, while 0.2 mL of normal saline was administered to the control group at ST36. Mortality, morbidity, general body and organ weight changes (after 4 weeks repeated dose), serum hematological and biochemical values, and histopathological changes in the liver and kidney were observed. Results: In both single and 4-week repeated dose toxicity experiments, no deaths or symptoms occurred in any of the groups. There were no significant differences between groups in terms of body and organ weights, serum hematological and biochemical values, and specific organ histopathological changes. Conclusion: ASN pharmacopuncture injection did not demonstrate significant toxicity in BALB/c mice compared with the control group, with a no-observed-adverse-effect level for a single dose of >125 mg/kg, and for 4 weeks repeated dose it was more than 62.5 mg/kg/day.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.25-36
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• 2000

No Abstract, See Full Text

• 한국임상수의학회지
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• 제24권4호
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• pp.608-612
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• 2007

4년령의 중성화한 수컷 코커 스파니엘이 후지 마비와 진전 때문에 내원 하였다. 본 환축은 X-ray와 MRI를 이용하여 추간판탈출증이 합병된 척수연화증으로 진단되었다. 환축의 치료를 위하여 봉독 약침, 뜸 및 한약제의 투여를 이용하였다. 사용 혈위는 GV02-1, GV04, GV11, GV12, GV13, GV14, BL11, BL18, BL23, BL25, BL28, GB21, HT07, KI03, LI04, LU07, SI03, SI06, ST36, ST38, ST39, ST40, ST41, GB30, GB31, GB34, LIV03, SP06 및 압통점($T1{\sim}T4\;and\;T9{\sim}L1$) 이었다. 그 결과, 1차 치료 시 환축은 기립이 불가능하였으며, 사지를 움직이지 못하였다. 그러나 7차, 14차 및 21차 치료 시 점차적인 임상증상의 개선이 관찰되었다. 비록 한방수의학적 방법으로 임상 증상이 완치된 것은 아니지만, 21차 치료 후 임상증상이 일부 호전된 증례이었다.

• 한국환경생태학회지
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• 제29권5호
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• pp.718-728
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• 2015

강릉남대천의 어류군집 변화와 도입된 어종의 서식실태를 파악하기 위해 9개 지점에서 2013년 9월에서 2014년 5월에 걸쳐 실시하였다. 채집된 어류는 총 14과 36종 이었고, 멸종위기종에 속하는 가시고기(Pungitius sinensis) 1종이 출현하였다. 한반도 고유종은 쉬리(Coreoleuciscus splendidus)을 포함하여 9종으로 고유화빈도가 25.0% 이었다. 우점종은 버들개(Rhynchocypris steindachneri, St. 1), 참갈겨니(Zacco koreanus, St. 2, 4, 5, 6), 새미(Ladislabia taczanowskii, St. 3), 피라미(Zacco platypus, St. 7, 8), 황어(Tribolodon hakonensis, St. 9) 이었다. 한강수계에서 강릉남대천으로 도입된 어종은 새미, 돌고기(Pungtungia herzi), 쉬리(Coreoleuciscus splendidus), 긴몰개(Squalidus gracilis majimae), 모래무지(Pseudogobio esocinus), 금강모치(Rhynchocypris kumgangensis), 피라미, 참갈겨니, 새코미꾸리(Koreocobitis rotundicaudata), 퉁가리(Liobagrus andersoni), 꺽지(Coreoperca herzi) 11종이었다. 개체군과 분포역이 급격히 확대되는 도입어종은 피라미, 참갈겨니, 돌고기, 긴몰개 등이었으며 정착되어 안정적인 개체군을 형성하였으며 서식지가 급격히 확대되지 않은 어종은 새미, 쉬리, 버들개 등 이었다. 정착하였으나 개체군이 매우 적은 어종은 꺽지와 퉁가리이었다. 1973년에 비해 개체수 구성비가 3%이상 감소된 어종은 버들개, 종개(Orthrias toni), 북방종개(Iksookimia pacifica), 은어(Plecoglossus altivelis), 가시고기, 꾹저구(Gymnogobius urotaenia) 등 이었고 버들개는 56.1%에서 12.9%로 가장 많이 감소하였다.

• 대한약침학회지
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• 제9권3호통권21호
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• pp.89-95
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• 2006

Objectives : This study is to research if any acupucture points are more effective, when Mahuang-chuanwu herbal acupucture is applied to the theatment of obesity. Methods : We searched oriental medical literatures and papers. Results : 1. Mahuang-chuanwu Herbal Acupuncture consists of distilled water of Ma-hunag and Chuan-wu, and it has the following effects : channel-warming, cold-dispering and eliminating damp. therefore it is applied to the treatment of obesity. 2. The acupuncture points, which have effects of channel-warming, cold-dispering and eliminating damp, are six : Kwanwon(Cv4), Kihae(CV6), Changmun(Liv13), Chok-samni(St36), Chung-wan(Cv12), Chonchu(St25). And Kwanwon(Cv4), Kihae(CV6), Changmun(Liv13), Chung-wan(Cv12), Chonchu(St25) locate on the abdomen. 3. Morbidly obese people, especially those in which their bellies are very large, are so because often the binding properties of the Taemaek(G26) have weakend. therefore Taemaek(G26) will be more effecive than Changmun(Liv13) that locate on right below the free end of the 11th rib, at the lateral side of the chest. 4. Mahuang-chuanwu Herbal Acupuncture will be most effective on the treatment of obesity with cold and damp as additional treatment, when it is injected into Kwanwon(Cv4), Kihae(CV6), Chonchu(St25), Taemaek(G26).

• 한국임상수의학회지
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• 제36권3호
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• pp.150-154
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• 2019

The purpose of this study was to investigate the etiological distribution, therapeutic outcome, and survival time in canine epilepsy. The medical records of 57 epileptic dogs were reviewed for the evaluation of etiological distribution. Among them, 27 dogs (47%) and 30 dogs (52%) had idiopathic epilepsy (IdE) and structural epilepsy (StE), respectively. Twenty-nine dogs (IdE: 16 dogs, StE: 13 dogs) were evaluated for therapeutic outcome and survival time. The incidence of generalized epileptic seizure (IdE, 56% vs. StE, 44%; P = 0.043) and the median seizure frequency at the time of first presentation (IdE, 2.0/month vs. StE, 13.3/month; P < 0.01) were significantly different between the two groups. Although pre-treatment seizure frequency and duration were not different, the median duration of seizure in the IdE group (0.5 min) was significantly shorter than that in the StE group (3 min) after treatment (P < 0.01). In addition, the median frequency of seizure was relatively lower in the IdE group (0.25/month) compared to the StE group (2.00/month) following antiepileptic therapy (P = 0.053). The median survival time of the IdE group (1.5 years [95% CI, 1.0-2.3 years]) was significantly longer than that of the StE group (0.4 year [95% CI, 0.2-1.3 years]) (P < 0.01). The information on etiological data and intracranial lesions may be useful for predicting treatment response and prognosis in epileptic dogs.

• Journal of Gastric Cancer
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• 제6권4호
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• pp.227-236
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• 2006

목적: 유전자 메틸화는 유전자의 서열에 영향을 주지 않으면서 유전자의 발현을 억제하고 세포분열 후 그대로 보존되는 후성적 변화이다. 위암조직과 정상위조직에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin 유전자와 MINT (MINT1, 2, 12, 25, 31)의 메틸화 상태를 검사하여 위암의 발생 과정에서의 작용과 CIMP 및 Helicobacter pylori균 감염을 포함한 임상병리학적인자와의 연관성을 알아보고자 하였다. 대상 및 방법: 위암과 정상위 신선 동결 조직 각각 36예를 대상으로 MSP (methylation-specific PCR)방법을 이용하여 메틸화 상태를 분석하였고 CIMP의 분석은 MINT1, MINT2, MINT12, MINT25, MINT31의 5개 marker를 대상으로 시행하였다. Helicobacter pylori균 감염여부는 Warthin-Starry silver 염색을 통하여 분류하였다. 결과: 위암 관련 유전자인 p14, p16, MGMT, COX-2, E-cadherin, hMLH1의 메틸화는 각각 14예(38.9%), 13예(36.1%), 8예(22.2%), 10예(27.8%), 21예(58.3%), 6예(16.7%)였다. MINT1과 MINT25의 메틸화는 위암조직에서 정상위조직에서보다 통계학적으로 유의하게 높게 관찰되었다. CIMP 양성률은 위암조직에서 44.4%로 높게 나타났으며 CIMP-H 위암은 환자의 연령과 종양크기와 연관이 있었다. CIMP 양성 위암은 p16 유전자의 메틸화와 연관이 있었고 p16 유전자의 메틸화는 조직학적으로 저분화, 미만형, 궤양형성하는 위암에서 낮게 나타났다. MINT1의 메틸화는 Helicobacter pylori균과 연관성이 있었다. 결론: 위암에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT (MINT1, 2, 12, 25, 31)의 불활성화에 DNA 메틸화가 작용함을 알 수 있었고, Helicobacter pylori균에 의한 위암발생에 MINT1의 메틸화가 연관이 있음을 알 수 있었다.

• Korean Journal of Acupuncture
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• 제36권1호
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• pp.19-35
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• 2019

Objectives : The purpose of this study is to review which acupoints and meridians are selected to treat visceral pain in articles published in international journals and to investigate quality of reporting acupuncture intervention according to the STRICTA guidelines. Methods : Electrical and hand search were conducted in PubMed, EMBASE, MEDLINE, and Cochrane Library for acupuncture studies on visceral pain in human and animals. Reporting quality of intervention was evaluated using the revised STRICTA guidelines. Results : We included 51 articles (7 clinical studies and 44 animal studies) in this study. The most frequently adopted meridians and acupoints were Stomach meridian, Spleen meridian, Conception Vessel meridian, and ST36, CV4, SP6, LI4, PC6, SP9, ST25, ST37 in clinical studies. In animal studies, Stomach meridian, Conception Vessel meridian, Pericardium meridian and ST36, ST37, CV12, PC6 were most frequently used. Animal studies showed significantly lower quality of reporting compared to clinical studies. According to the analysis on the effect of year of publication of articles, announcement of STRICTA guidelines did not improve the quality of reporting for studies on visceral pain. Conclusions : These results suggest that both proximal and distal acupoints based on meridian theory were used to study the underlying mechanisms of visceral pain both in human and animal studies. Guidelines of acupuncture intervention reporting for animal study is necessary to improve the quality of evidence, and it will also allow us the integrative understanding of the mechanisms and clinical effects of acupuncture treatment in human and animal.

• Korean Journal of Acupuncture
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• 제33권4호
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• pp.166-175
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• 2016

Objectives : This article examines whether 'Samni', which appears 62 times in Dongui-Bogam: Treasured Mirror of Eastern Medicine, means Joksamni(ST36) or Susamni(LI10). Methods : After searching the sentences which include 'Samni' in Dongui-Bogam using Medical Classics Database, we checked the original reference of them to find clues to identification of Samni. As for the statements not identified by the original reference nor by Dongui-Bogam, we judged the meaning of Samni by comparing diseases of the statements with those of Joksamni or Susamni's treatment area. Acupoints used together with Samni were also taken into account. Results : Out of 52 statements, Samni in 25 statements turned out to be Joksamni after tracing the reference; 6 statements mention stomach, and 4 statements treat Samni as an acupoint of stomach meridian; in 12 statements, diseases belong to Joksamni's treatment area. Samni of the remaining 5 statements also seem to be Joksamni since the diseases of the statements are similar to those of Joksamni. It implies that Joksamni's treatment area has expanded over time. Conclusions : All Samni in Dongui-Bogam is Joksamni.