• Journal of Chest Surgery
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• v.26 no.2
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• pp.75-79
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• 1993

Myocardial protection against ischemic and reperfusion injuries is still in troublesome eventhough couples of the way of myocardial protection have been applied since 1970's. One of the possibility in myocardial protection is adding Fructose-l,6-diphosphate(FDP) in cardioplegic solution. It is assumed that FDP can promote ATP production under anaerobic condition as well as inhibiting the supressing effect of lactate on phosphofructokinase. We compared the myocardial protecting effects of FDP in crystalloid cardioplegic solution (St. Thomas formula, 10$^{\circ}C$, pH = 7.4) and reperfusate using isolated rat hearts in modified Langendorf apparatus by the parameters of preischemic and post reperfusing heart rate, time to first beat, occurance of arrhythmia, time to stabilization, and the rate of left ventricular pressure developing. Group A (n = 10), containing no FDP in cardioplegic and reperfusing solutions was control. Group B (n = 5), containing FDP in cardioplegic solution, showed statistically significant superiority of postischemic left ventricular pressure development than the control group. Group C (n = 5), containing FDP in reperfusate, showed statistically significant myocardial depressing effect than the controls. Other parameters were unremarkable. The cause is uncertain, but it is assumed that the negative feedback inhibition of FDP in energy metabolism or unknown blocking effect of FDP on certain transmembrane ionic currents is present. In conclusion, 1) FDP in cardioplegic solution has beneficial effect on postischemic left ventricular preservation. 2) FDP is strong acid when is hydrolyzed, so precise acid titration is neccessary. 3) FDP in reperfusate has negative left ventricular preservation, otherwise the mechanism is still uncertain.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1381-1386
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• 2014

In spite of variation in coat color, size, and production traits among indigenous Bangladeshi cattle populations, genetic differences among most of the populations have not been investigated or exploited. In this study, we used a high-density bovine single nucleotide polymorphism (SNP) 80K Bead Chip derived from Bos indicus breeds to assess genetic diversity and population structure of 2 Bangladeshi zebu cattle populations (red Chittagong, n = 28 and non-descript deshi, n = 28) and a semi-domesticated population (gayal, n = 17). Overall, 95% and 58% of the total SNPs (69,804) showed polymorphisms in the zebu and gayal populations, respectively. Similarly, the average minor allele frequency value was as high 0.29 in zebu and as low as 0.09 in gayal. The mean expected heterozygosity varied from $0.42{\pm}0.14$ in zebu to $0.148{\pm}0.14$ in gayal with significant heterozygosity deficiency of 0.06 ($F_{IS}$) in the latter. Coancestry estimations revealed that the two zebu populations are weakly differentiated, with over 99% of the total genetic variation retained within populations and less than 1% accounted for between populations. Conversely, strong genetic differentiation ($F_{ST}=0.33$) was observed between zebu and gayal populations. Results of population structure and principal component analyses suggest that gayal is distinct from Bos indicus and that the two zebu populations were weakly structured. This study provides basic information about the genetic diversity and structure of Bangladeshi cattle and the semi-domesticated gayal population that can be used for future appraisal of breed utilization and management strategies.

• Journal of Korean Physical Therapy Science
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• v.24 no.2
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• pp.45-52
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• 2017

Purpose : Most of the moderns have been spent sedentary life style in front of desk or on the chairs, so that their hamstring muscles are shorted commonly and the shortness of hamstring muscles are caused to some problems in functional activities. The purpose of this study was to compare the effectiveness of thermotherapy with hot-pack and ultrasound on the tightness of hamstring muscles in individuals with hamstring tightness. Method : Twenty-eight persons with hamstring tightness participated in this study. They were randomly assigned to three exercise groups: stretching group(n=10), hot-pack group(n=9), and ultrasound group(n=9). Before experiment, this study measured the angle of hip flexion using a inclinometer in all participants. Stretching group conducted actively stretching on their shorted leg for 30 seconds, 3 times, and rest for 10 seconds between each exercises. Hot-pack group have applied hot-pack($70{\sim}75^{\circ}C$) on shorted hamstring muscles for 20 minutes before stretching exercise. Ultrasound group have applied therapeutic ultrasound(1 MHz and 1.5W/cm2) on shorted hamstring muscles for 10 minutes before stretching exercise. All data was analysed using one-way repeated ANOVA. Result : The results revealed that ultrasound therapy before stretching is more effective to the flexibility on hamstring muscles than hot-pack before stretching and stretching only. Conclusion : The results of this study show that thermotherapy with therapeutic ultrasound will be effective on the shortness of hamstring muscles before stretching exercise.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.407-414
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• 1997

In this study, the optimum cutting 6equency and level of mineral nitrogen fertilization were investigated for the production of Reed canarygrass in uncultivated rice paddy. The results are summarized as follows; 1. Higher relative dry matter yields were recorded in 2nd cut of plots with 3 and 5 cutting frequencies, and 3rd cut of plot with 4 cutting kquency, respectively. 2. With no nitrogen fertilization, mean dry matter yields per year were 6.2~7.6 tonsha and the highest yield appeared in plot with 4 cutting 6equency. 3. The increased fertilization of mineral nitrogen resulted in the increased dry matter yield. Significantly higher dry matter yields than that of no nitrogen fertilization were recorded in fertilization of 90 kg nitrogen per year in 3 cutting fequency, 240 kg in 4 cutting frequency and 150 kg in 5 cutting 6equency respectively. 4. Efficiency of dry matter production with nitrogen fertilization(kg DMkg N) was higher in 30kg Nhalcut in 3 and 5 cutting eequency, 60kg Nhalcut in 4 cutting 6equency respectively. In each cutting kequency, the higher efficiency of dry matter production appeared in 1st cut in 3 cutting kequency, and 2nd cut in 4 and 5 cutting 6equency respectively. 5. Economic N level(kg/ha) was 179.5~242.3kg/ha in 3 cutting 6equency, and 189.6-241.6kg/ha and 167.0 ~253.2kg/ha in 4 and 5 cutting 6equency respectively, and marginal dry matter yields were 11.4~ 12.3 tons/ ha, 11.2 ~ 11.8 tons/ha and 8.3 ~9.1 tons/ha in 3, 4 and 5 cutting 6equency respectively. 6. The limiting N fertilization level to the highest dry matter were estimated to be 569.9kg/ha, 492.4kg/ha and 654.lkg/ha in 3, 4 and 5 cutting kequency respectively.

• Genomics & Informatics
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• v.13 no.4
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• pp.137-145
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• 2015

Selective sweep can cause genetic differentiation across populations, which allows for the identification of possible causative regions/genes underlying important traits. The pig has experienced a long history of allele frequency changes through artificial selection in the domestication process. We obtained an average of 329,482,871 sequence reads for 24 pigs from three pig breeds: Yorkshire (n = 5), Landrace (n = 13), and Duroc (n = 6). An average read depth of 11.7 was obtained using whole-genome resequencing on an Illumina HiSeq2000 platform. In this study, cross-population extended haplotype homozygosity and cross-population composite likelihood ratio tests were implemented to detect genes experiencing positive selection for the genome-wide resequencing data generated from three commercial pig breeds. In our results, 26, 7, and 14 genes from Yorkshire, Landrace, and Duroc, respectively were detected by two kinds of statistical tests. Significant evidence for positive selection was identified on genes ST6GALNAC2 and EPHX1 in Yorkshire, PARK2 in Landrace, and BMP6, SLA-DQA1, and PRKG1 in Duroc. These genes are reportedly relevant to lactation, reproduction, meat quality, and growth traits. To understand how these single nucleotide polymorphisms (SNPs) related positive selection affect protein function, we analyzed the effect of non-synonymous SNPs. Three SNPs (rs324509622, rs80931851, and rs80937718) in the SLA-DQA1 gene were significant in the enrichment tests, indicating strong evidence for positive selection in Duroc. Our analyses identified genes under positive selection for lactation, reproduction, and meat-quality and growth traits in Yorkshire, Landrace, and Duroc, respectively.

• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.554-559
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• 1999

The purpose of this study was to evaluate effect of chitosan-oligosaccharides (CHIOL) on hydrophobicity of pathogenic E coli including a field isolate from suckling piglet with diarrhea, E coli-O157 : H7, and E coli-O149 : K88ac. E coli field isolate appeared adhesion of 100% to n-hexadecane between 0.00125% and 0.05% CHIOL. E coli-O157 : H7 occurred adhesion of 69% and 64% under the level of 0.00125% and 0.025% CHIOL, respectively. E coli-O149 : K88ac showed adhesion of 100% in higher than 0.025% CHIOL. For cationic action, the adhesion of E coli isolate and E coli-O149 : K88ac to n-hexadecane were inhibited at level of higher than 10mM $Ca^{2+}$ but did not induce any difference among the concentrations used(p < 0.01). However, the adhesion of E coli-O157 : H7 to n-hexadecane was inhibited at level of higher than 50mM $Ca^{2+}$. In a field trial, control piglets showed average mortality of up to 58% during 3 days after the onset of diarrhea. In contrast, the prevalence of E coli-induced diarrhea in CHIOL-treated groups without mortality was dropped down to average 34% on the 1st day after the treatment of CHIOL, and average 2% on the 4th day. After then, piglets with diarrhea was not present. In conclusion, the low concentrations of CHIOL were most likely to associate with the enhancement of hydrophobicity to pathogenic E coli. Calcium inhibited the hydrophobicity of E coli by CHIOL. These results suggested that CHIOL could be played an efficient and reliable role in treating enteric colibacillosis of piglets.

• Journal of the Society of Naval Architects of Korea
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• v.30 no.2
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• pp.132-140
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• 1993

The design of LNG ship needs very high level structural design/analysis technology compared with conventional ship types because it requires perfect security against the extremly dangerous and cryogenic cargo. Hence, present paper describes the general procedure of the structural safety assessment for independent tank type LNG ship, which contains following items. 1) Long term prediction of the wave induced stresses including ship motion analysis, structural analysis of hull and tank and stochastic analysis process of ocean waves. 2) Fatigue strength analysis of a tank structure based on the S-N approach. 3) Structural safety assessment against the fatigue crack propagation based on the LBF(Leak Before Failure) concept. The first report focuced on the item (1) (2) and example calculation was performed on a prototype LNG ship. The remained part will be covered by the second report.

• Archives of Pharmacal Research
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• v.10 no.4
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• pp.212-218
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• 1987

Intact brain cell aggregates were dissociated from adult rat brains without cerebellum using a sieving technique. This proparation was used to elucidate the binding characteristics of agonist to muscarinic acetylcholine receptors (mAchR) in brain. Incubation of cells with carbamylcholine (carbachol) was shown agonist-induced receptor down-regulation depending on the concentration of agonist, not depending on the incubation time. This effect of carbachol was due to a reduction in the maximal binding capacity ($B_{max}$) to the mAchR without decreasing the affinity of the remaining receptors in incubation at 37.deg.C but was not apparent inincubation at $15^{\circ}}C$In addition, it was abolished when the receptors were blocked by atropine. The decline in ($^3H$)N-methylscopolamine (($^3H$)NMS) binding induced by agonist was reflected as a significant reduction in the receptor density with no change in receptor affinity, suggesting that 'true' receptor down-regulation takes place. Moreover, when the receptors were labeled with the lipophilic antagonist ($^3H$) quinuclidinyl benzilate (($^3H$) QNB) insted of the hydrophilic ligand ($^3H$)NMS, the magnitude of the observed receptor down-regulation was significantly lower in case of the former than the latter. This suggested that exposure of intact brain cells to muscarinic agonists might induce a slight degree of accumulation of receptors in intracellular sites before the receptors are actually degraded.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.4
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• pp.13-24
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• 2008

Objectives : The purpose of this study was to investigate the effect of Poncirus Trifoliata(PT) on improvement of fecal impaction in spinal cord injured(SCI) rats. Methods : Fifteen adult Sprague-Dawley female rats were used weighing 200~250 g. A complete spinal cord transection was performed surgically at the T10 cord level. Experimental groups were assigned into 3 groups: Control(n=5), SCI+vehicle(n=5) and SCI+PT(n=5). PT was administered 100mg/kg in 0.5ml every 24 hours from 1st operation day to 7th day. We measured the body weight and food intake as well as the number and the weight of fecal pellet every morning. After 1 week of operation, whole colon was divided into proximal and distal segments under anesthesia. Each segment of colon was mounted with longitudinal direction in a organ bath. We measured spontaneous contraction and compared the area under the curve in each segments. Enhanced responses were observed by acetylcholine($10^{-6}M$), 40 mM KCl solution, L-NAME($10^{-4}M$). Results : The fecal number and weights were significantly higher in the group of SCI+PT than SCI+vehicle group(p<0.05). In organ bath study, area under the curves of the spontaneous contraction in SCI+vehicle and SCI+PT groups were significantly increased compared to control group. Contractility of distal colon in response to acetylcholine or KCl in SCI+vehicle group was significantly decreased compared to other groups(p<0.05). Conclusions : These results suggest that PT might be useful to promote bowel emptying in spinal cord injured rats.

• Journal of Aquaculture
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• v.16 no.4
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• pp.252-256
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• 2003

Effluent from farm trefish contained high concentrations of nitrogen and phosphorus, such kinds of nutrients were released to the environment without proper treatment and thses increased the pollution of the environment. We evaluated the conventional treatment system with cost effective ecotechnologies for the removal of nutrients. Water lettuce chambers were investigated under the various experimental conditions to improve the efficiency of N&P removal and the treatment of from aquaculture effluent. In this research, six water lettuce chambers (80 liter each) received combination of aquaculture wastewater effluent at hydraulic retention times (HRTs) of 1, 2, 4 and 8days. The water lettuce chambers operated at a 8 day HRT investigated for aquaculture effluent (1'st) showed average removal efficiency, BOD, T-N, T-P of 92.8, 79.0 and 93.6% on average respectively.