• Plant Resources
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• 제7권2호
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• pp.93-103
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• 2004

Genetic background and phylogenetic relationships among 20 Korean wheat cultivars were assessed using microsatellites after amplifying with 13 SSR primer pairs. Average allele number per primer pair was 3.36. Genetic similarities for every pair of cultivars ranged from 0.42 to 0.97, with 0.69 of overall average. Korean cultivars were divided into two major groups based on microsatellite DNA polymorphisms. Group I consisted of relatively old cultivars developed until 1970s, and group II contained the recent cultivars developed during 1980s and 1990s. Amongst old elite cultivars/lines, ‘Yukseung 3’, ‘Norin 12’ and ‘Norin 72’ contributed most to the genetic background of cultivars belonging to group I, and ‘Norin 4’, ‘Norin 12’, ‘Norin 43’ and ‘Norin 72’ to group II, respectively. The phylogenetic relationship of Korean wheat cultivars was in accordance with the genealogical data of each cultivar. The genetic background of each cultivar was assessed from the point of breeding and germplasm management such as variety identification and duplicated accessions for assisting in developing a system for the registration of new variety based on the molecular characterization in future.

• 생명과학회지
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• 제17권1호
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• pp.24-30
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• 2007

꽃치자(Gardenia jasminoides var. radicans)와 열매치자(C. jasminoides for. grandiflora)는 Gardenia속으로는 우리나라에 두 분류군밖에 없으며 열매치자는 약용, 식용, 꽃치자는 방향제로 쓰인다. 그런데 꽃이 지고 나면 형태적으로 구분이 거의 되지 않는다 ISSR분석으로 이들 종의 유전적 다양도와 집단구조를 실시하였다. 88개의 DNA 분절에서 한 분류군에만 나타나는 특이밴드가 탐지되었다. 한국의 세 야생 집단은 분리되어 있고 패치 분포를 보이지만 재배종에 비해 높은 유전적 다양성을 유지하친 있었다. 열매치자가 꽃치자보다 유전적 다양성이 높았으며 ISSR 마커로 이들 분류군이 잘 분리되었다. 또한 야생 집단이 재배 집단보다 다양성이 약간 높으나 유의성은 없었다. 이는 재배화과정에서 유전적 다양성의 일부 상실이 있었으나 인위적인 채취와 식재로 야생 집단과 재배 집단의 유전적 교류가 존재하였음을 시사한다.

• 농업과학연구
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• 제39권4호
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• pp.477-482
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• 2012

Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at $15^{\circ}C$ was measured to represent low-temperature germination and used for QTL analysis. The germination rate at $15^{\circ}C$ for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.

• 한국자원식물학회지
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• 제28권6호
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• pp.697-703
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• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Journal of Microbiology
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• 제42권2호
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• pp.103-110
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• 2004

In order to understand the molecular basis of selective expression of stationary-phase genes by RNA polymerase containing$\sigma$$\^$38/ (E$\sigma$$\^$38/) in Escherichia coli, we examined transcription from the stationary-phase promoters, katEP, bo1AP, hdeABP, csgBAP, and mcbP, in vivo and in vitro. Although these pro-moters are preferentially recognized in vivo by E$\sigma$$\^$38/, they are transcribed in vitro by both E$\sigma$$\^$38/ and E$\sigma$$\^$70/ containing the major exponential $\sigma$, $\sigma$$\^$70/. In the presence of high concentrations of glutamate salts, how-ever, oldy E$\sigma$$\^$38/ was able to efficiently transcribe from these promoters, which supports the concept that the promoter selectivity of $\sigma$$\^$38/-containing RNA polymerase is observed only under specific reaction con-ditions. The examination of 6S RNA, which is encoded by the ssr1 gene in vivo, showed that it reduced E$\sigma$$\^$70/ activity during the stationary phase, but this reduction of activity did not result in the elevation of E$\sigma$$\^$38/ activity. Thus, the preferential expression of stationary-phase genes by E$\sigma$$\^$38/ is unlikely the con-sequence of selective inhibition of E$\sigma$$\^$70/ by 6S RNA.

• 한국정보통신학회논문지
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• 제13권12호
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• pp.2671-2676
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• 2009

EMD(Empirical mode decomposition) 방법은 시간-주파수 분석의 새로운 방법으로 적응적이며 효율적으로 신호를 분해한다. EMD는 신호 그 자체에 의해 정의된 IMFs(Intrinsic mode functions)로 명명되는 함수의 집합으로 분해되며, 분해된 IMFs는 원신호의 고유한 속성을 보존하므로 기저함수 및 필터로 사용될 수 있다. EMD 방법에 의한 분해는 신호의 지역적인 시간 스케일 특성에 기반을 두고 있으므로 비선형(non-linear) 비정상(non-stationary) 신호처리에 적합하며 ECG와 같은 생체 신호처리에 유용하다. 본 논문은 EMD 방법을 이용하여 ECG 신호를 분해하고 분해된 신호의 특성을 이용하여 잡음 제거 필터를 구현하였다. 전통적인 저주파 필터가 퓨리에 변환을 이용하여 주파수 영역에서 신호를 해석하는 것과 달리 EMD 방법은 시간 영역에서 필터링하여 신호의 속성을 유지한다. 영상 향상의 정도를 측정하기 위한 PRMD와 SSR 평가지수를 사용하여 제안된 기법과 전통적인 저주파 필터의 결과를 비교 제시하였다.

• 한국산업정보학회논문지
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• 제5권3호
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• pp.38-43
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• 2000

능동 레이더 반사기는 지금까지는 특히 비행물체와 미사일의 레이더 유효 반사 면적을 효과적으로 증가시키기 위한 군사적 응용에만 사용이 제한되어져 왔기 때문에 그다지 친숙한 용어는 아니다. 레이더 트랜스폰더의 가장 잘 알려진 응용은 군용의 피아 식별 장치 (IFF ; Identification Friend or Foe)와 이것의 민간 항공부문이고, 항공 교통 관제(ATC : Air Traffic Control)를 위한 2차 감시 레이더(SSR ; Second Surveillance: Radar), 그리고 보다 최근에는 전 세계 해상 조난 및 안전 제도(GMDSS : Global Maritime Distress and Safety System)의 수색 구조용 트랜스폰더(SART : Search And Rescue Transponder)등이 있다. 최근 연안이나 대양에서의 해난사고가 빈번히 발생되고 있으므로, 이로 인한 해양오염이 심각한 실정이다. 이제는 전통적인 항해표지 및 시설로써 해상교통의 안전을 유지하기에는 충분치 못하며 새로운 개념과 구조가 적용되어야만 한다. 따라서 본 연구에서는 개선된 기능을 가지는 능동 레이더 반사기를 제안하고 제작하였다. 제작된 시스템은 기존의 시설에 비해 높은 성능 향상을 보인다.

• 한국육종학회지
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• 제40권3호
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• pp.243-249
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• 2008

In the previous study, 141 $BC_3F_2$ lines from a cross between the Oryza sativa cv. Milyang 23 and O. glaberrima were used to identify favorable wild QTL alleles for yield component traits. In this study, we carried out QTL analysis of four grain morphology as well as four yield component traits using 141 $BC_3F_5$ lines from the same cross and compared QTLs detected in two different generations. The mean number of O. glaberrima segments in the 141 $BC_3F_5$ lines ranged from 1 to 13 with 2.69 and 5.71 of the average means of homozygous and heterozygous segments, respectively. There was a three-fold difference in the number of QTLs detected for four traits commonly evaluated in two generations (seven QTLs in the $BC_3F_5$ vs 21 in the $BC_3F_2$ population). The percentages of the phenotypic variance explained by QTLs in the BC3F5 population were similar to or less than those in the $BC_3F_2$ population. This is probably due to the difference in the genetic composition of two populations and the environmental effects. The locations of the QTLs commonly detected in both generations were in good agreement except for one QTL for spikelets per panicle. The yield QTL, yd3 was colocalized with the spikelets per panicle, spp3. Yield increase at this locus is due to the increase in spikelets per panicle, because both traits were associated with increase in spikelets per panicle and yield due to the presence of an O. glaberrima allele. Clusters of QTLs for grain morphology traits were observed in two chromosome regions. One cluster harboring five QTLs near SSR markers RM106 and RM263 was detected on chromosome 2. This population would serve as a foundation for development of the introgression line population from a cross between Milyang 23 and O. glaberrima.

• 한국육종학회지
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• 제40권3호
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• pp.250-257
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• 2008

Knowledge of genetic diversity and of the genetic relationships among elite breeding materials has had a significant impact on the improvement of crops. In maize, this information is particularly useful in i) planning crosses for hybrid and line development, ii) in assigning lines to heterotic groups and iii) in plant variety protection. We have used the SSR technique to study the genetic diversity and genetic relationships among 76 Korean waxy corn accessions, representing a diverse collection from throughout Korea. Assessment of genetic diversity among members of this group was conducted using 30 microsatellite markers. Among these 30 microsatellite markers, we identified a total of 127 alleles (with an average of 4.2 and a range of between 2 and 9 alleles per locus). Gene diversity at these 30 microsatellite loci varied from 0.125 to 0.795 with an average of 0.507. The cluster tree generated with the described microsatellite markers recognized two major groups with 36.5% genetic similarity. Group I includes 63 inbred lines, with similarity coefficients of between 0.365 and 0.99. Group II includes 13 inbred lines, with similarity coefficients of between 0.45 and 0.85. The present study indicates that the 30 microsatellite loci chosen for this analysis are effective molecular markers for the assessment of genetic diversity and genetic relationships between Korean waxy corn accessions. Specifically, this study's assessment of genetic diversity and relationships between a set of 76 Korean waxy corn inbred lines will be helpful for such activities as planning crosses for hybrid and line development and association mapping analyses of maize breeding programs in Korea.

• 한국육종학회지
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• 제43권4호
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• pp.322-329
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• 2011

국내 육성된 보리 품종의 유전적 다양성을 조사하기 위하여 microsatellite marker의 이용 가능성에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 보리 70품종을 20개의 microsatellite marker를 이용하여 분석하였을 때 대립유전자의 수는 2~9개로 비교적 다양한 분포를 나타내었으며 전체 124개의 대립유전자가 분석되었다. PIC 값은 0.498~0.882 범위에 속하였으며 평균값은 0.734로 나타났다. microsatellite marker를 이용하여 작성된 보리70품종의 품종간 유전적 거리는 0.10~0.91의 범위로 나타났고, 유사도 지수 0.15를 기준으로 할 때 70개 품종은 2조 보리 그룹과 6조 보리 그룹으로 나눌 수 있었으며, 공시 품종 모두 microsatellite marker의 genotype에 의해 뚜렷이 구분되었다. 이 연구결과는 보리의 유전적 다양성 및 품종식별을 위한 기초 자료로 유용하게 이용될 수 있는 것으로 사료된다.