• 한국식생활문화학회지
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• 제27권1호
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• pp.66-74
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• 2012

It is well known that sunflower (SF) sprout has more beneficial effects than SF seed due to increased levels of phytochemical components such as vitamins, total phenolics, and isoflavones during germination. This study investigated the physicochemical properties and antioxidative activities of SF seed during both germination and cultivation. In a proximate analysis, the water content of SF groat was 9.17% and then increased to 15.32% on the 11th day after seeding. On a dry weight basis, crude fat content decreased while the contents of carbohydrates, crude protein, and crude ash increased. As cultivation proceeded, the contents of minerals were in decreasing order of K, Mg, Ca, Na, Zn, Fe, Mn, and Cu, in which SF sprout cultivated for 9 or 11 days contained the highest mineral contents. Though vitamin C was not detected on SF groat, the content of vitamin C continuously increased up to the 5th day of cultivation and then decreased gradually. Vitamin E content in SF groat was higher than that in SF sprout. It was also found that the vitamin E content in SF sprout was the highest on the 5th day of cultivation. Daidzin was not detected in SF groat, but its concentration reached a maximum on the 5th day of cultivation in SF sprouts. Furthermore, higher amounts of daidzein were observed on the 3rd, 5th, and 9th days of cultivation. The highest total isoflavone content was observed on either the 3rd or 5th day of cultivation. The highest content of total phenolics was observed on the 5th of cultivation. When DPPH radical and peroxyl radical scavenging activities of SF sprout were measured in order to measure antioxidant efficacy, it was found that 5 day-cultivated SF sprout had the highest scavenging activities. In conclusion, SF sprout cultivated for 9 or 11 days was found to be a good source of minerals. Furthermore, the fifth-day after seeding was the optimal time for the production of SF sprout with effective natural antioxidant activity and high amounts of functional components such as vitamins, total phenolics, and isoflavones.

• 한국작물학회지
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• 제48권4호
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• pp.292-296
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• 2003

The lateral root formation in soybean sprout culture declines its quality. This study was done to measure the effect of fluorescent light treatment during 24 hour imbibition and 6-day culture on seed germination and growth of soybean sprout. After 6 day culture, the sprouts were sorted as normal (＞4cm), abnormal (＜4cm) and non-germination by their hypocotyl lengths, and lateral roots, fresh and dry weights were measured. Lateral roots were less formed in the fluorescent light treatment lasted during the whole period of the imbibition than in the treatment for 50 minutes a day during the culture. The fluorescent light treatment during the imbibition mainly affected the germination and growth compared to the treatment done during the culture. Compared to the dark imbibition, the light treatment during the imbibition resulted in more normal sprouts, thicker diameters of hypocotyl and hook, and more fresh weights in cotyledon, hypocotyl, whole sprout, and economic yield. However, these results were reverse in lengths of hypocotyl and root, and fresh and dry weights of roots. It is concluded that the fluorescent lamp mainly irradiating red and blue lights can be used for the sprout production as an alternative light replacing blue and red lights treated during the imbibition because it blocked the lateral root appearance and stimulated growth of the sprout.

• 한국식품영양학회지
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• 제33권5호
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• pp.459-472
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• 2020

The purpose of this study was to evaluate the physicochemical components and antioxidant activities of wheat sprout powder prepared by the enzymatic hydrolysis and lactic acid bacteria treatment. The four kinds of pre-treatment were: no treatment (WP), treated with enzyme (WPE), treated with lactic acid bacteria (WPL), and treated with enzyme and lactic acid bacteria (WPEL) were applied to the wheat sprout powder. The WPEL had higher total free amino acid and essential amino acid content than the other samples. As for the volatile aroma of the wheat sprout powder, 29 types of compounds were identified in the WP and WPL, 28 types in the WPE, and 27 types in the WPEL, respectively. The total polyphenols and flavonoids contents, in the wheat sprout powder was enhanced with the enzyme and the lactic acid bacteria pre-treatment. The WPEL had highest DPPH radical scavenging activities. The overall acceptability was the highest at 6.24 points in the WPEL. Based on these observations, it was confirmed that the enzyme and lactic acid bacteria pre-treatment could improve the antioxidant activities and active component of the wheat sprout powder.

• 한국작물학회지
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• 제53권3호
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• pp.303-307
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• 2008

Cotyledon Black Decay (CBD) on soybean sprout mimics the black spot due to microbial infection. CBD, not visible or predictable at seedlot state, for some reason, shows up exclusively on cotyledon of soybean sprout during sprouting process. Such an incidence rate fluctuated from 0.8 to 19.5% over three years from 2004. We suspected some pod-infecting anthracnose fungi and/or pod-blight pathogen, or pod-sucking bean bug, one of the major pests of soybean, might have involved, of which we ruled out fungal pathogen because it was preventable through heat treatment, a proven method for seedlot disinfestation. The healthy seeds artificially fed by bean bug for one to seven days were sprouted, and 6 to 41% of the soybean sprout revealed the CBD mimic to those occurred in soybean sprout from previous commercial seedlot screening experiments. This finding is the first report to confirm that bean bug damage to pod at $R_8$ stage is directly responsible for the CBD, which did not concur with any other deleterious effects on sprouting such as reduction in hypocotyls elongation and rooting except unsightly sprout quality. However, earlier feeding either at green pod or greenish yellow pod stage ($R_6$ -early $R_7$ stage) resulted in rather severe damages, which strikingly reduced hypocotyls growth to about one forth to about two third, as well as the reduction in rates of seed germination.

• 한국식품영양과학회지
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• 제46권7호
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• pp.841-847
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• 2017

본 연구에서는 새싹보리를 첨가하여(1, 2%) 스트링치즈를 제조하고 품질 특성을 검토하였다. pH는 새싹보리를 첨가한 스트링치즈에서 낮은 값을 보였으며 첨가 농도에 따른 유의적 차이는 보이지 않았다. 아미노산 함량은 새싹보리를 첨가함에 따라 threonine, cysteine, tyrosine, arginine, alanine, proline, lysine, isoleucine, leucine, glutamic acid 등의 함량이 비례적으로 증가하였고, 총 아미노산 함량도 대조구에 비하여 새싹보리 2% 첨가 치즈에서 높았다. 색도는 L*(명도)와 a*(적색도)에서 새싹보리 첨가량이 증가함에 따라서 낮아지는 경향을 보였으며, b*(황색도)는 증가하는 경향을 보였다. 물성의 경우 새싹보리 첨가량이 증가할수록 경도와 검성, 씸힙성은 유의적으로 높게 나타났으며, 반대로 응집성은 낮아지는 경향을 보였다. 또한, 신전성 측정 결과 새싹보리를 첨가하지 않은 스트링치즈보다 새싹보리 첨가 스트링치즈의 신전성이 증가함을 확인하였다. 총 페놀 함량은 새싹보리 2% 첨가 치즈에서 유의적으로 높은 값을 보였으며, 총 플라보노이드와 DPPH 라디칼 소거능은 유의적 차이를 보이지 않았다.

• 한국응용과학기술학회지
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• 제33권1호
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• pp.13-22
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• 2016

밀싹 추출물의 생리활성 및 화장품 소재로서의 가능성 여부를 규명하고자 하였다. 본 연구는 세포실험을 통해 밀싹 추출물의 피부 세포에 대한 독성을 확인하고, 피부 세포 미백 활성 및 노화에 대한 연구를 수행하여 기능성 화장품 소재로서의 가능성을 알아보고자 하였다. 본 연구 결과 밀싹 추출물이 HDF, B16F10 세포에 대한 독성이 적은 것으로 확인되었다. 멜라닌 생합성 억제에 대한 효과는 약한 것으로 확인되었으나, 자외선에 유도되는 MMP-1의 발현을 저해함으로써 피부의 광노화를 억제하는 효과를 통해 광노화에 의한 피부 주름과 자연 노화에 의한 피부 주름을 예방하는데 유의한 효과를 가질 수 있음을 확인하였다. 따라서 본 연구는 밀싹 추출물의 기능성 화장품 소재로 사용 시 피부 노화 예방 관점에서의 기능성 화장품 소재로 매우 유용하게 활용될 수 있을 것으로 사료된다.

• 한국식생활문화학회지
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• 제30권1호
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• pp.77-85
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• 2015

This study investigated the physicochemical properties and antioxidative activity of soymilk added with buckwheat sprout (SBS), and sensory properties of SBS were analyzed with control soymilk (CS). Moisture content decreased while protein, ash, and lipid contents increased according to content of buckwheat sprout. Solids content and viscosity tended to increase with addition of buckwheat sprout from 0% (CS) to 2.5% (2.5SBS), 3.5% (3.5SBS), and 4.5% (4.5SBS). SBS was found the significant pH drop from 7.08 to 6.43, 6.34, and 6.21. Suspension stability of soymilk slightly decreased with addition of buckwheat sprout. Hunter's color value L of SBS decreased while a and b values increased in comparison with CS. Rutin content was measured 23.78 mg/100 g (2.5SBS), 39.68 mg/100 g (3.5SBS), and 44.80mg/100 g (4.5SBS). Vitamin C content in SBS was higher than CS. Daidzin content increased as buckwheat sprout was added. Total phenolic content increased from 100.95 mg/100 g (CS) up to 315.71 mg/100 g (4.5SBS). For free radical scavenging activity, SBS was significantly higher than CS. In conclusion, SBS can be used as a functional food with higher amounts of rutin, vitamin C, total phenolics, and daidzin. 2.5SBS and 3.5SBS also showed better overall quality characteristics.

• 한국자원식물학회지
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• 제34권4호
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• pp.395-402
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• 2021

식품산업 활용성을 고려하여 땅콩과 땅콩새싹의 열수와 주정 추출물을 제조하고 추출물내 resveratrol과 aspartic acid 함량을 UPLC-MS/MS를 이용하여 분석한 결과 땅콩새싹의 주정 추출물이 가장 높은 resveratrol과 aspartic acid 함량을 나타내었다. 또한 땅콩새싹의 주정 추출물은 3T3-L1 세포에 처리시 지방세포분화를 유의적으로 억제하였다. 따라서 땅콩새싹의 주정추출물은 기능성식품소재로 활용 가능성이 높을 것으로 판단된다. 향후 이러한 결과를 바탕으로 땅콩의 발아조건 및 추출 시간과 온도에 따른 활성성분 함량을 측정하여 최적의 활성성분 추출조건을 확립하는 연구를 진행할 계획이다.

• 한국동물생명공학회지
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• 제38권4호
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• pp.189-198
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• 2023

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2, Gsr, HO-1, and catalase, were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2, HO-1, and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

• 한국식품저장유통학회지
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• 제22권4호
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• pp.482-489
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• 2015

본 연구는 갈대의 이용 가치성을 높이고자 수행하였으며, 갈대 부위와 증자 처리에 의한 이화학적 특성 변화를 조사하였다. 이에 따른 결과는 아래와 같다. 수분은 무증자 처리 시료와 증자처리 시료 모두 어린순이 뿌리 보다 높았다. 조회분은 무증자처리 시료에서는 어린순이 증자처리 시료에서는 뿌리가 높은 함량을 보였고, 조단백질 함량은 무증자처리 시료와 증자처리시료 모두 어린순이 뿌리 보다 높았다. 조지방은 무증자처리 시료에서 어린순과 뿌리의 함량이 0.67%이었으나, 증자처리 시료에서 어린순이 뿌리보다 비교적 높은 함량을 보였고, 조섬유 함량은 무증자처리 시료와 증자처리 시료 모두 어린순이 뿌리 보다 높았다. 가용성무질소물은 무증자처리 시료와 증자처리 시료 모두 뿌리가 어린순에 비해 2배 이상 높은 함량을 보였다. 유리당 총 함량은 무증자처리 시료와 증자처리 시료 모두 뿌리가 어린순 보다 높은 함량을 보였으며, 유기산 또한 유리당과 같은 경향을 나타내었다. 무기성분은 어린순과 뿌리 모두 무증자처리 시료와 증자처리 시료 모두 Ca과 K의 함량이 높게 나타났다. 갈대의 총 유리아미노산은 무증자처리 시료와 증자처리 시료 모두 어린순이 뿌리보다 높았으며, 이중 serine이 주요 아미노산으로 나타났다. vitamin C 함량은 무증자처리 시료에서 어린순이 61.74 mg%로 뿌리의 4.54mg% 보다 높은 함량을 나타내었고, 증자처리 시료에서는 함량이 감소하였으나 어린순이 뿌리보다 높은 함량을 보였다. Total polyphenol 함량은 무증자처리 시료와 증자처리시료 모두 어린순이 높은 함량을 보였다. DPPH free radical scavenging activity은 무증자처리 시료와 증자처리 시료를 비교 할 때 크게 차이는 나지 않았으나, 뿌리가 어린순에 비해 높은 활성을 보였다.