• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.6
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• pp.413-417
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• 2000

In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium, Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1-1 $\mu\textrm{g}$/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.1
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• pp.116-123
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• 2004

The demand for the stable and comfortable cabin of a high speed passenger ship is increasing. The study on shipboard comfort has been mainly concentrated on the motion control of a whole hull body. In this study, however, a new control system operated by two parallel robots (3RPS, 3SPR) such as the active suspension system of motor vehicle is proposed. The goal of this control is keeping zero velocity of the upper robot (cabin) although the lower robot (ship) is moving by the waves. Jacobian matrix was used to design the controller, From the simulation results, the remarkable reduction of motion of the cabin (upper platform) was observed. The 3SPR parallel robot shows better performance compared to the 3RPS robot.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.02a
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• pp.200-208
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• 2003

미생물수를 측정하는 가장 정확하고 보편적인 방법은 배양법이다. 그러나 배양법은 많은 시간과 노력이 소요되기 때문에 현장에서 실시간에 준하는 측정법으로 사용하기에는 원천적으로 한계를 가지고 있다. 이러한 한계를 극복하기 위해서는 실시간(real-time) 또는 준실시간(near real-time)으로 미생물을 검출할 수 있는 기술이 요구된다. 표면플라즈몬공명(surface plasmon resonance: SPR)은 생물분자 또는 미생물을 실시간 또는 준실시간으로 검출할 수 있는 센서로서 특이성(specificity)과 정확도(accuracy) 측면에서 일찍이 큰 관심을 받아왔다. 1902년, Wood(1902)는 반사 회절격자를 사용하여 연속광원의 스펙트럼을 관찰한 결과, 회절광 스펙트럼에서 어두운 좁은 밴드를 발견하였으며, Fano(1941)는 이 현상이 surface plasma waves와 관련이 있음을 이론적으로 밝혔다. (중략)

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.1
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• pp.156-160
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• 2007

Surface plasmon resonance(SPR) has been widely studied for biological and chemical sensing applications. The present study conducts numerical simulation for the single and bimetallic layer SPR configurations by using the multiple beam interference matrix(MBIM) method to investigate the influence of wave interference and complex refractive indices of materials on optical characteristics such as reflectance and optical phase shift which are used for sensing. First, calculated reflectances are compared with experimental data for validation. In addition, in the single film structures this study finds out the appropriate film thicknesses with minimum reflectance for cases of gold film and silver film. For a bimetallic silver-gold film structure, in particular, the bimetallic film thicknesses that has the minimum reflectance are found 36 nm for silver and 5 nm for gold. From the results, the use of phase shift would be useful compared to reflectance in determining the SPR configuration because the phase shift becomes more sensitive than reflectance.

• International Journal of Automotive Technology
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• v.7 no.5
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• pp.565-570
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• 2006

The development of a light-weight vehicle is in great demand for enhancement of fule efficiency and dynamic performance. The vehicle weight can be reduced effectively by using lightweight materials such as aluminum and magnesium. However, if such materials are used in vehicles, there are often instances when different materials such as aluminum and steel need to be joined to each other. The conventional joining method, namely resistance spot welding, cannot be used in joining different materials. Self-piercing rivet(SPR) and adhesive bonding, however, are good alternatives to resistance spot welding. This paper is concerned with the crushing test of double hat-shaped member made by resistance spot welding, SPR and adhesive bonding. Various parameters of crashworthiness are analyzed and evaluated. Based on these results, the applicability of SPR and adhesive bonding are proposed as an alternative to resistance spot welding.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.5
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• pp.922-926
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• 2010

We have investigated the grating coupled surface plasmon resonance (GC-SPR) sensors using ZnO nano-grating structures to enhance the sensitivity of an SPR sensor. The GC-SPR sensors were analyzed using the finite-difference time-domain method. The optimum resonance angles of 49 degrees are obtained in the 150 nm wide grating structure with a period of 300 nm for the ZnO thickness of 30 nm. Then, the ZnO nano-grating patterns were fabricated by using laser interference lithography. The measured resonance angle of nano-grating patterns was around 49 degrees. Here, an enhanced evanescent field is obtained due to the surface plasmon on the edge of the bandgap when the ZnO grating structures are used to excite the surface palsmon.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.3
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• pp.433-436
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• 2012

We propose a novel micro surface plasmon resonance (SPR) sensor system based on polymer materials. The proposed SPR system consists of the incident medium with polymer waveguide and the gold thin film for sensing area. Using a polymer optical waveguide instead of a prism in SPR sensing system offers miniaturization, low cost, and potable sensing capability. The whole device performance was analyzed using the finite-difference time domain method. The optimum gold thickness in the attenuated total reflection mirror of polymer waveguide is around 50 nm and the resonance angle to generate surface plasmon wave is 68 degrees.

• BMB Reports
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• v.31 no.1
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• pp.101-105
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• 1998

Immunoassay of botulinum toxin (BTX) B type was investigated using two typed of biosensors: light addressable potentiometric sensor (LAPS) and surface plasmon resonance (SPR) sensor. Urease-tagged and immuno-filtration capture method have been used for LAPS. Tag-free and direct binding real-time detection method have been used for SPR sensor. The detection limit of sandwich assay format with LAPS was 10 ng/ml, which was the lowest among methods tested. SPR has the advantage of being more convenient because tag-free direct binding assay can be used and reaction time was reduced, regardless of low sensitivity. This result shows that sandwich assay format with LAPS can be used as an alternative method of BTX mouse bioassay which is known as the most sensitive method for the detection of BTX.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.52 no.9
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• pp.520-525
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• 2003

A robust controller for large space structures(LSS) is studied from passivity point of view. While velocity sensors are commonly used for proportional-derivative (PD) control law to stabilize large space structures, if the structure can be controlled without velocity measurements, it is desirable against the failure of velocity sensors and for the cost reduction of the sensing system. In a recent result a dynamic output feedback control law has been provided using only displacement measurements. This paper presents a passivity-based controller design method and provides an alternative stability analysis tool for the previous displacement feedback robust control law. The closed-loop system can be viewed as a feedback interconnection of a passivated large space structure (LSS) and a strictly positive real (SPR) system.

• Interaction and multiscale mechanics
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• v.6 no.2
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• pp.257-270
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• 2013

The use of lightweight materials has been steadily increasing in the automotive industry, and presents new challenges to material joining. Among many joining processes, self-piercing riveting (SPR) is particularly promising for joining lightweight materials (such as aluminum alloys) and dissimilar materials (such as steel to Al, and metal to polymer). However, to establish a process window for optimal joint performance, it often requires a long trial-and-error testing of the SPR process. This is because current state of the art in numerical analysis still cannot effectively resolve the problems of severe material distortion and separation in the SPR simulation. This paper presents a coupled meshfree/finite element with a moving boundary algorithm to overcome these numerical difficulties. The simulation results are compared with physical measurements to demonstrate the effectiveness of the present method.