• 원예과학기술지
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• 제33권6호
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• pp.845-853
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• 2015

The objective of this study was to predict the timing of nitrogen (N) demand through analyzing chlorophyll fluorescence (ChlF), soil-plant analysis development (SPAD), and normalized difference vegetation index (NDVI), which are positively correlated with foliar N concentration in star cluster (Pentas lanceolata). The plants were grown in potting soil under optimal conditions for 30 d, followed by weekly irrigation with five concentrations (0, 4, 8, 16, and 24 mM) of N for an additional 30 d. These five N application levels corresponded to leaf N concentrations of 2.62, 3.48, 4.00, 4.23, and 4.69%, respectively. We measured 13 morphological and physiological parameters, as well as the responses of these parameters to various N-fertilizer treatments. The general increases in Dickson's quality index (DQI), above-ground dry weight (DW), total DW, flowering rate, ${\Delta}F/Fm$', and qP in response to treatment with 0 to 8 mM N were similar to those of SPAD, NDVI, and Fv/Fm. Consistent and strong correlations ($R^2$= 0.60 to 0.85) were observed between leaf N concentration (%) and SPAD, NDVI, ${\Delta}F/Fm$', and above-ground DW. Validation of leaf S PAD, NDVI, and ${\Delta}F/Fm$' revealed that these vegetation indices are accurate predictors of leaf N concentration that can be used for non-destructive estimation of the proper timing for N-solution irrigation of P. lanceolata. Moreover, irrigation with 8 mM N-fertilizer i s recommended w hen leaf N concentration, SPAD, NVDI, and ${\Delta}F/Fm$' ratios are reduced from their saturation values of 4.00, 50.68, 0.64, and 0.137%, respectively.

• 한국포장학회지
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• 제27권3호
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• pp.181-186
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• 2021

결구상추의 수확후 물올림을 위한 처리를 위해서 육묘 시에 부직포 포트를 이용하여, 저장 시 선도 유지 효과를 검토하였다. 결구상추 육묘 시 프러그 트레이와 부직포 포트를 사용한 결과 수확 시 생육에 차이가 보이지 않았다. 따라서 부직포 포트를 이용한 육묘 방법이 포장 및 저장 방법 개선을 위한 물올림 처리에 이용할 수 있는 것으로 판단되었다. 수확후 결구상추의 경정(줄기)을 부직포로 감아 물올림을 실시한 후 포장하여 저장하면, 무포장은 생체중이 감소하는데 비해 물올림 처리는 저장 28일에 1.7%까지 증가하였다. 그 외 엽록소(SPAD), 색도(hue angle)에서 물올림 처리의 측정치가 무처리 보다 높은 결과를 보였다. 따라서 결구상추의 선도유지를 위한 효과적인 저장방법으로 물올림 처리가 효과적인 것으로 보이며, 물올림을 위한 포장의 편이를 위하여 육묘 방법의 개량 가능성을 확인하였다. 앞으로 다른 원예작물에서도 저장 후 선도를 개선하기 위해서는 재배에서부터 수확후 품질에 중점을 둔 추후의 연구가 더 필요할 것으로 사료된다.

• Plant Breeding and Biotechnology
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• 제1권1호
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• pp.1-8
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• 2013

Low-temperature stress is an important factor controlling the growth and development of rice (Oryza sativa L.) in temperate region. In this study, a molecular linkage map consisting of 136 SSR markers was employed to identify QTL associated with cold tolerance at the seedling stage. 80 recombinant inbred lines (RILs) from an intersubspecific cross between Milyang23 (O. sativa ssp. Indica) and Hapcheonaengmi3, a japonica weedy rice and the parents were evaluated for leaf discoloration and SAPD value of seedlings. Rice plants were grown for 15 days in the low-temperature condition (13/20℃ day/night) and the control condition (25/20℃ day/night) in the growth chamber. The degree of leaf discoloration showed a highly significant correlation with the SPAD value in the low-temperature plot (r = -0.708, P < 0.0001). A total of four QTLs for SPAD were identified and the phenotypic variance explained by each QTL ranged from 5.4 to 16.0%. Two QTLs detected in the control condition were located on chromosomes 2 and 5, respectively. Two QTL on chromosomes 1 and 4 were detected at the low-temperature condition and Hapcheonaengmi3 alleles increased the SPAD values at these loci. Substitution mapping was conducted to delimit the position of qSPA-4 using introgression lines derived from the same cross. Results indicated that qSPA-4 was located in a 810-Kb region flanked by RM16333 and RM16368. The results indicated that Hapcheonaengmi3 contains QTL alleles that are likely to improve cold tolerance of Indica rice.

• 한국조경학회지
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• 제46권1호
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• pp.1-8
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• 2018

본 연구는 광량이 산호수(Ardisia pusilla), 크루시아(Clusia rosea), 팔손이(Fatsia japonica) 목본식물 3종의 초기 생육 반응에 미치는 영향을 구명하고자 수행하였다. 목본식물 3종을 10cm 포트에 식재한 다음, 광량 15, 30, 60, $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 4수준으로 광조사 시간은 12/12(명기/암기)로 설정하여 120일 동안 실시하였다. 4주 간격으로 초고, 엽장, 엽폭, 엽록소형광(Fv/Fm), SPAD, Hunter 값의 생육측정과 실험 종료 후 식물체 지상부와 지하부의 생체중과 건조중을 측정하였다. $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 실험기간동안 초고, 엽면적, 지상부와 지하부의 생체중과 건조중 값이 가장 크게 나타났다. 팔손이는 광량이 높을수록 엽장, 엽폭이 컸으며, 그 밖의 식물에서는 광량에 따른 유의적인 차이가 나타나지 않았다. 산호수의 Fv/Fm 값은 60, $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 광량에 대한 스트레스를 받는 것으로 나타났으나, 그 밖의 식물과 광량에서는 Fv/Fm는 모두 정상 범위로 나타나, 스트레스를 받지 않았다. SPAD값은 $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 크루시아만 유의적인 차이를 보였으며, 그 밖의 식물과 광량에서는 유의적인 차이가 나타나지 않았다. 산호수의 Hunter 값은 모든 광량에서 유의적인 차이가 없었으며, 크루시아는 $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서, 팔손이는 $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 L, a, b값에 특이성이 나타났다. $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 산호수 줄기의 성장 폭이 컸으며, 크루시아는 60과 $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 지속적인 성장을 보여, 적정수준의 초고를 유지하기 위해서 이들은 $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$이하의 광량이 필요할 것으로 판단된다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.254-261
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• 2000

Soil chemical properties, relief of field surface, SPAD values and grain yield were investigated in a 0.5ha paddy field in 1999 to obtain basic field information for precision agriculture. Descriptive statistics of field information showed that the coefficient of variation ranged from 1.63% to 38.7%. Field information showed a high spatial dependence for within paddy field. The ranges of spatial dependence were from 15m to 60m, respectively. Kriged maps enable the visualization and comparison the spatial variability of field information. The causes of spatial variability of the field information could be explained rationally by a field management map. Grain yield was negatively correlated with pH, relief values, whereas, was positively correlated with total C, total N, C/N ratio, mineralizable N, available P and exchangeable K, Ca at the significant level of 1 %.

• Agricultural and Biosystems Engineering
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• 제6권1호
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• pp.1-7
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• 2005

Assessing health condition of crop in the field is one of core operation in precision fanning. A sensing system was proposed to remotely detect the crop health condition in terms of SP AD readings directly related to chlorophyll contents of crop using a multispectral camera equipped on ground-based platform. Since the image taken by a camera was sensitive to changes in ambient light intensity, it was needed to convert gray scale image data into reflectance, an index to indicate the reflection characteristics of target crop. A reference reflectance panel consisting of four pieces of sub-panels with different reflectance was developed for a dynamic calibration, by which a calibration equation was updated for every crop image captured by the camera. The system performance was evaluated in a field by investigating the relationship between com canopy reflectance and SP AD values. The validation tests revealed that the com canopy reflectance induced from Green band in the multispectral camera had the most significant correlation with SPAD values $(r^2=0.75)$ and NIR band could be used to filter out unwanted non-crop features such as soil background and empty space in a crop canopy. This research confirmed that it was technically feasible to develop a ground-based remote sensing system for assessing crop health condition.

• 한국농림기상학회지
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• 제23권3호
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• pp.163-168
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• 2021

광은 식물 광합성에 반드시 요구되는 에너지이다. 차광의 종류를 전면차광과 부분차광으로 구분하고, 각각의 차광 조건에서 생육한 콩의 엽록소 형광을 관측하여 광합성능을 평가하였다. 전면차광에서는 SPAD값으로 대표되는 엽록소 농도와 광이용효율을 표현하는 ETR (Electron Transport Rate)이 크게 낮아졌다. 차광 박스 제거 후에 SPAD와 ETR 모두 대조구와 같은 정도가 되었으나, 열 소산 기작을 나타내는 NPQ (Non-Photochemical fluorescence Quenching)는 높아졌다. 이렇게 전면차광을 겪었던 콩의 광이용효율은 회복했지만, 높아진 NPQ가 광인산화 효율을 떨어뜨리므로 실제 노지 광합성량은 필연적으로 낮아질 것이다. 부분 차광에서도 SPAD와 ETR이 대조구와 큰 차이를 보이지 않았으나, NPQ는 높은 모습을 보였다. 따라서, 도시 농업 또는 영농형 태양광과 같은 부분차광 조건의 광합 성량은 단순히 작물의 광이용효율과 누적 광 에너지량으로 계산한 추정값보다 작을 것으로 예상된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.67-67
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• 2020

Though there is an on-going need for the diversity of ornamental plants for in-door environment, their growth and stress adaptability in comparatively low light intensity condition require further studies for implementation. Here investigates the growth and chlorophyll fluorescence parameters of Farfugium japonicum (L.) Kitam, Liriope muscari (Decne.) L.H.Bailey and Acorus gramineus Aiton under several light intensities which were based on common in-door environment. The growth measurement of the plants included the quantity, length, width and SPAD value of the leaves. Calculated values of Fm/Fo, Fv/Fm, Pi_Abs, ETo/RC and DIo/RC were used as the parameters of the chlorophyll fluorescence under 10, 50, 100 and 200 PPFD (μmol·m^-2·s^-1) light intensities. In-door plants group was put in a closed room allowing no other light sources for 10 weeks and the control group was put in glass-greenhouse for the same period. The overall in-door growth of L. muscari was not significantly different in all light intensities compared to the control group and even showed the higher SPAD values. Also, an increasing tendency of Pi_Abs value under 10 to 100 PPFD was observed implying that L.muscari could adapt well to in-door environment. Measurement of A. gramineus growth mostly showed the highest values in the control group especially in the number of the leaves. Nevertheless, chlorophyll fluorescence parameters showed no significant value difference between in-door and the control groups and thus, A. gramineus might have possibility of successful adaptation to in-door environment. F. japonicum showed deficient growth in plant height and leaf length compared to the control but, it seemed to be able to sustain ornamental value under in-door light intensities. Furthermore, Pi_Abs and DIo/RC values were increased under in-door light conditions suggesting potential adaptability of F. japonicum.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.335-335
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• 2017

Recently NERICA (New Rice for Africa) was developed by a crossing of African rice (Oryza glaberrima Steud.) and Asian rice (Oryza sativa L.) in West Africa, and is considered to be drought resistant, but drought resistance of NERICA and differences between Asian rice are not clarified enough. In this research, NERICA (four cultivars and two lines), Asian rice (three cultivars and sativa parent of NERICA) and African rice (glaberrima parent of NERICA) were cultivated in the field in Shizuoka University under drought and traits of each cultivar and line relevant to drought resistance, stomatal conductance by porometer, soil water content of individual depths by TDR method, SPAD values by SPAD meter and leaf thickness by micrometer, were measured and compared with dry matter production and yield. Effects of compost were also compared among sativa parent, one NERICA cultivar and two NERICA lines. Glaberrima parent showed highest top dry weight. One NERICA line, one drought resistant Asian rice cultivar and sativa parent, showed higher top dry weight and yield (ear weight) than other Asian rice cultivars and NERICA cultivars and line tested. Compost tended to increase top dry weight and yield in one of NERICA line and sativa parent. But in one NERICA cultivar and line, top dry weight and yield were not increased. In one of Asian rice, one of NERICA line and sativa parent that showed high top dry weight and yield, stomatal conductance was high. On the contrary the glaberrima parent and in other NERICA cultivars and line it was low. In sativa parent compost increased stomatal conductance but in NERICA cultivar and lines it was not. Among cultivars and lines that showed high top dry weight and yield sativa parent and one of NERICA line SPAD value and leaf thickness were high but in one of Asian rice and glaberrima parent they were low. Cultivar and line differences in yield and top dry weight among Asian rice and NERICA were significantly correlated with those in stomatal conductance ($r=0.778^{**}$ and $r=0.654^*$, respectively) and those in leaf thickness ($r=0.600^*$ and $r=0.640^*$, respectively). In Asian rice cultivars average soil water content was significantly correlated with yield ($r=0.886^*$) but in NERICA cultivars and lines it was not significant correlated (r= -0.256). Cultivar and line differences in leaf thickness were significantly correlated with SPAD value ($r=0.773^{**}$). In Asian rice cultivars it was significantly correlated ($r=0.962^{**}$), but in NERICA cultivars and lines it was not significantly correlated (r=0.559). Asian rice cultivars tended to consume soil water to increase yield but in NERICA cultivars and lines the tendency was not clear. Correlation between SPAD value and leaf thickness was different between Asian rice and NERICA cultivars and lines, and in Asian rice cultivars it was significantly correlated but in NERICA cultivars and lines it was not significant. Importance of maintaining high stomatal conductance by high leaf thickness was clarified.

• 화훼연구
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• 제17권4호
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• pp.237-241
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• 2009

본 연구는 난과 식물의 아황산가스 피해증상을 정확하게 알기 위해 팔레놉시스, 심비디움, 온시디움을 이용하여 밀폐된 챔버에서 수행하였다. 가스처리는 아황산가스 농도를 0, 5, 10, 25, 50 ppm으로 하였고, 노출시간은 18시간, 온도 $25{\pm}5$, 습도 $50{\pm}5%$로 하였다. SPAD를 이용하여 가스처리 전후의 엽록소 함량을 측정하였으며, 피해엽율과 이온유출량을 조사하였다. 팔레놉시스의 경우 10ppm에서 피해엽율이 23.3%로 나타났고 심비디움, 온시디움의 경우 25ppm에서 각각 4.0, 4.4%의 피해가 나타나서 저항성이 강하다고 판단되었다. 주요증상은 초기에 잎의 뒷면이 수침상으로 나타났다가 급격히 잎이 황색으로 변하거나 탈색된다. 이온유출량은 아황산가스 농도가 높을수록 심하게 나타났고, 엽록소 함량은 아황산가스 농도가 높을수록 낮아졌다.