• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.429-432
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• 1990

The promoter isolated from chromosomal DNA of an alkali-tolerant Bacillus sp. YA-14 was subcloned and biochemically characterized. Also the relationships between the promoter activity and sporulation were investigated. In alkali-tolerant Bacillus sp. and Bacillus subtilis, the activity of promoter began to increase at the onset of sporulation with the same mode, and repressed in the presence of 1.0% (wtv) glucose. Among five spoO genes, three epoO genes (spoOB, spoH, spoOJ) were required for promoter expression.

• The Korean Journal of Zoology
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• v.9 no.1
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• pp.31-38
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• 1966

굴둑거미속(Cybaeus)은 물거미과(Argyronetidae)에 속하는 거미로서 지금가지 전세계에서 약 62종이 기재되었는데 그 지리적 분포를 보면 신북구에 40종, 구북구에 18종, 신열대구에 3종, 에치초피아구에 1 종의 순으로 되어 신북구가 절대 다수인 67%를 차지하고 있다. 구북구의 16종은 구주산이 5종, 몽고산이 1 종, 중국 본토산이 1 종, 일본산이 11종 기재되어있다. 한국산 굴둑거미속에 대해서는 저자 (1962)가 소백산산의 Cybaeus sp. 1(2 ♀♀)과 Cybaeus sp. 2(2Y♀♀)를 , 남궁씨(1964)가 충주산의Cybaeus sp.를 보고하였을뿐이다. 금반 한국산 굴둑거미속의 거미를 정리한 결과 저자가 보고한 Cybaeus sp.1과 남궁씨가 보고한 Cybaeus sp. 는 동일종으로서 신종에 해당함을 알았으며 이 밖에도 1 신종과 1 미기록종을 얻었기에 이에 기재하였다. 즉 미기록종 : Cybaeus nipponicus (Uyemura, 1938) 신종 : Cybaeus lingus sp. nov. , Cybaeus triangulus sp. nov.

• Journal of Life Science
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• v.18 no.12
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• pp.1723-1727
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• 2008

Chlorella sp. CMS-1 strain was isolated from the outdoors cultivation pools in Culmansa Co., Ltd. This strain was found to be a rounded type of 3 ${\mu}m$. Phylogenetic analysis by the 18S rRNA sequencing using isolated strain is most similar to Chlorella sp. IFRPD 1018 gene at the level of nucleotide sequence identity at 99%. Accordingly, the isolated Chlorella strain was named as Chlorella sp. CMS-1 based on its morphological and phylogenetic properties. The concentrations of crude protein and fat were 59% and 0.01%, respectively. Major compositional amino acids (mg%) were glutamic acid 6.21, alanine 5.76, aspartic acid 5.44%, glycine 4.29%, and threonine 3.09% and major free amino acids (mg%) were ${\gamma}$-aminobutyric acid (GABA) 7.13%, L-alanine 1.44%, L-glutamic acid 0.90, L-leucine 0.26% and L-glycine 0.20%. The concentrations of major minerals were P 2.25%, K 2.25%, Na 1.09%, Mg 0.63%, and Ca 0.28%.

• Tuberculosis and Respiratory Diseases
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• v.60 no.6
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• pp.638-644
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• 2006

Backgrounds: This study investigated whether or not a polymorphism in the gene encoding the surfactant protein A(SP-A) has any bearing on the individual susceptibility to the development of chronic obstructive pulmonary disease(COPD) in a genetically homogenous Korean population. Methods: The genotypes of 19 COPD patients and 20 healthy neonates as controls were tested using a polymerase chain reaction followed by restriction fragment length polymorphism analysis for the SP-A gene. Results: The specific frequencies of the 6A2 and 6A18 alleles of SP-A1 and the 1A2 allele of SP-A2 were much higher in the COPD group than control group (p<0.05). However, the frequencies of the 6A3 and 6A4 alleles of SP-A1 and the 1A0 allele of SP-A2 in the COPD group were significantly lower than the control group. In the COPD group, the frequencies of the +50 locus genotypes GG of SP-A1 and the +9 locus genotypes CC of SP-A2 were 85.0% and 60.6%, respectively, and 19.7% and 24.8% in the control group, respectively. The frequencies of the polymorphic genotypes or alleles showed a statistically significant difference between the COPD group and the control group (P<0.05). Conclusion: A genetic polymorphism in SP-A is associated with the development of COPD in the Korean population.

• Korean Journal of Environmental Biology
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• v.23 no.4
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• pp.367-373
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• 2005

We conducted a year-long survey in 2000 to examine seasonal fluctuations in the abundance of the demersal copepod Pseudodiaptomus sp., the dominant copepod in the Seomjin River estuary, where the spring tide strongly affects changes in salinity gradients. Pseudodiaptomus sp. was found throughout the year in the entire range of salinities measured, but most individuals appeared at oligohaline conditions below 5.0 psu, and less than $2\%$ were observed in polyhaline conditions above 18.0 psu. The peak abundance occurred during autumn in oligohaline waters, and the density was relatively low during the rainy season in summer. In spring and autumn, copepodites were most abundant in oligohaline waters, although they were also fairly abundant in mesohaline conditions $(5\~18\;psu)$. Females with egg sacs appeared in oligo- and mesohaline waters during spring and autumn but were seldom found in polyhaline conditions throughout the year. Our results indicate that, despite the strong physical influence of the tide, Pseudodiaptomus sp. can manipulate its position to remain at its preferred salinity. We also found that spawning mainly occurred in oligohaline waters twice a year.

• Smart Structures and Systems
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• v.18 no.4
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• pp.801-818
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• 2016

To estimate structural displacement, a visually servoed paired structured light system (ViSP) was proposed in previous studies. The ViSP is composed of two sides facing each other, each with one or two laser pointers, a 2-DOF manipulator, a camera, and a screen. By calculating the positions of the laser beams projected onto the screens and rotation angles of the manipulators, relative 6-DOF displacement between two sides can be estimated. Although the performance of the system has been verified through various simulations and experimental tests, it has a limitation that the accuracy of the displacement measurement depends on the alignment of the laser pointers. In deriving the kinematic equation of the ViSP, the laser pointers were assumed to be installed perfectly normal to the same side screen. In reality, however, this is very difficult to achieve due to installation errors. In other words, the pose of laser pointers should be calibrated carefully before measuring the displacement. To calibrate the initial pose of the laser pointers, a specially designed jig device is made and employed. Experimental tests have been performed to validate the performance of the proposed calibration method and the results show that the estimated displacement with the initial pose calibration increases the accuracy of the 6-DOF displacement estimation.

• Journal of Environmental Science International
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• v.18 no.4
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• pp.435-443
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• 2009

A bacterial strain SY-97 that showed algicidal activity against Cochlodinium polykrikoides was isolated from coastal water of Uljin (eastern coast of Korea) in August, 2005. The isolated strain was identified as Brachybacterium sp. by morphological and biological tests, and analysis of 16S rDNA sequence. The optimal culture conditions for the growth of strain SY-97 were $30^{\circ}C$, initial pH 7.0, and salinity 2.0%. From the result of cell culture insert experiment, Brachybacterium sp. SY-97 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.2{\times}10^4\;cells/m{\ell}$) cultures, 100% of C. polykrikoides cells was destroyed within 15 hours. The released algicides were heat-tolerant to $100^{\circ}C$ and stable in pH $6.0{\sim}10.0$. These results suggest that Brachybacterium sp. SY-97 is potentially useful for controlling outbreaks of C. polykrikoides.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.18-18
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• 2015

Wilt disease in Oak trees occurs during summer season in Korea. Mass attack of trees by an ambrosia beetle (Platypus koryoensis) was the characteristic feature before appearance of the wilting symptoms. Raffaelea sp. caused the discoloration of xylem area called as wound heartwood. Raffaelea sp. was observed both on the body surfaces and inside the mycangia of the beetle Platypus sp. The scanning electron microscope (SEM) analysis showed that fungal spores were present within the wall of gallery and vessels that formed tyloses. The results revealed that the water movement in vessels was blocked as the fungus started to grow which caused the formation of tyloses thereby resulting wilt symptoms. We found that both female and male beetle Platypus sp. had fungi on their bodies and their large and small mycangia. This study confirmed that the fungus was transferred to oak trees by Platypus sp.

• The Journal of Natural Sciences
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• v.7
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• pp.59-65
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• 1995

A bacterium which produce hyaluronic acid complex was isolated from soil, and identified as Klebsiella sp. L-10. The maximal hyaluronic acid complex production was obtained when the strain was cultured at $37^{\circ}C$ for 18hrs with shaking in the optimal medium containing 0.1% yeast ext., 3% tryptone, 5% glucose, 10mM $K_2HPO_4$and $KH_2PO_4$, respectively and initial pH6.5 and the final hyaluronic acid complex production under the above condition was 1,400mg per liter of cultures.

• Journal of Digital Convergence
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• v.18 no.12
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• pp.285-292
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• 2020

The purpose of this study is a descriptive investigation study to find out the knowledge, awareness and performance of the nurse's standards precaution(SP) and to identify the factors affecting the performance of them. The research method was collected through a questionnaire from nurses at two general hospitals in B city and analyzed with technical statistics, t-test, ANOVA, Person's correlation coefficient and Multiple regression using the SPSS program. As a result of the average level of knowledge of the SP 22.55/25, recognition 3.68/4, and performance 3.77/4. The recognition of SP and implementation of SP(r=.250, p=.003) had a significant amount of correlation. The influence factor on the implementation of the SP was recognition of the SP(β=-.582, p=.001). Therefore, in order to improve the performance of SP and to respond well to new respiratory infections, accurate awareness of the SP of nurses and opportunities for systematic education and participation in education are needed by medical institutions.