• Plant Biotechnology Reports
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• 제4권2호
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• pp.149-155
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• 2010

Expression of the genes for carotenoid bio-synthesis (crt) is dependent on light, but little is known about the underlying mechanism of light sensing and signalling in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis). In the present study, we investigated the light-induced increase in the transcript levels of Synechocystis crt genes, including phytoene synthase (crtB), phytoene desaturase (crtP), ${\zeta}$-carotene desaturase (crtQ), and ${\beta}$-carotene hydroxylase (crtR), during a darkto-light transition period. During the dark-to-light shift, the increase in the crt transcript levels was not affected by mutations in cyanobacterial photoreceptors, such as phytochromes (cph1, cph2 and cph3) and a cryptochrome-type photoreceptor (ccry), or respiratory electron transport components NDH and Cyd/CtaI. However, treatment with photosynthetic electron transport inhibitors significantly diminished the accumulation of crt gene transcripts. Therefore, the light induction of the Synechocystis crt gene expression is most likely mediated by photosynthetic electron transport rather than by cyanobacterial photoreceptors during the dark-to-light transition.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2004년도 수산관련학회 공동학술대회 발표요지집
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• pp.513-513
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• 2004

Manila clam, Ruditapes philippinarum, is commercially and ecologically important marine bivalve in Korea and japan. However, clam landings in the two countries have dramatically declined since the 1980-1990's. In the present study, the protozoan parasite, Perkinsus sp., lectin (host's defense-related glycoprotein) and histopathological features were investigated in Manila clams collected from Gomso Bay in Korea and Ariake Bay in japan (one of the largest clam beds in each country) during summer and fall, 2002-2003. DNA sequences of non-transcribe spacer (NTS), internal transcribed space. (ITS) and 5.85 rRNA of Perkinsus sp. were identical to those of P. atlanticus that was reported in Europe and Korea. For diagnosis of Perkinsus, the fluid thioglycollate medium (FTM) and the 2 M NaOH lysis methods were used. Prevalence of the parasite varied from 92.5-98.7% in Gomso Bay and 35.5-37.9% in Ariake Bay. Infection intensity, in terms of the number of Perkinsuscells per gram tissue wet weight, in the clams of Gomso Bay in fall 2002 averaged 1,010,077-470,937 recording approximately100 times higher than that of Ariake Bay, and these were twice higher than those of summer samples in each location. Mean hemagglutination titer of the clams from Gomso Bay was approximately 60-folds higher than that of clams from Ariake Bay in 2002. In histological preparation of the clams from Gomso Bay in 2002, trophozoites of P. atlanticus were in groups and resulted in severe inflammatory response of host clam. Prevalence of the trematod, Cercaria tapes-like in the clams of Gomso Bay and Ariake Bay were 8.8 % and 10.5% respectively. In conclusion, the clams from Gomso Bay showed more severe pathologic symptoms and higher immune response than those of the clams from Ariake Bay.

• Plant Biotechnology Reports
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• 제4권4호
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• pp.329-334
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• 2010

Multiple sequence alignments showed that the prolines at the 25th, 129th, 153rd, 242nd, 322nd, and 434th amino acids in 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium sp. strain CP4 are strongly conserved in various prokaryotic EPSPS proteins. Single point mutations of the conserved prolines to alanine (P25A, P153A, P242A, P322A, and P434A) were introduced in the CP4 EPSPS in order to investigate the importance of the conserved prolines for the enzyme properties. The point mutations caused decreases in substrate binding affinity and catalytic efficiency as well as the glyphosate resistance, in general. Especially, the 25th and 242nd prolines located in the polypeptide hinges connecting top and bottom domains of CP4 EPSPS as well as the 434th proline at the C-terminus of the enzyme turned out to be crucial for the enzyme activity.

• Journal of applied mathematics & informatics
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• 제30권3_4호
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• pp.571-591
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• 2012

To the best of our knowledge, there is no method, in the literature, to find the fuzzy optimal solution of fully fuzzy shortest path (FFSP) problems i.e., shortest path (SP) problems in which all the parameters are represented by fuzzy numbers. In this paper, a new method is proposed to find the fuzzy optimal solution of FFSP problems. Kumar and Kaur [Methods for solving unbalanced fuzzy transportation problems, Operational Research-An International Journal, 2010 (DOI 10.1007/s 12351-010-0101-3)] proposed a new method with new representation, named as JMD representation, of trapezoidal fuzzy numbers for solving fully fuzzy transportation problems and shown that it is better to solve fully fuzzy transportation problems by using proposed method with JMD representation as compare to proposed method with the existing representation. On the same direction in this paper a new method is proposed to find the solution of FFSP problems and it is shown that it is also better to solve FFSP problems with JMD representation as compare to existing representation. To show the advantages of proposed method with this representation over proposed method with other existing representations. A FFSP problem solved by using proposed method with JMD representation as well as proposed method with other existing representations and the obtained results are compared.

• 대한한방소아과학회지
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• 제27권4호
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• pp.10-16
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• 2013

Objectives The purpose of this study was to investigate the acupuncture therapy about Attention Deficit Hyperactivity Disorder (ADHD) in China. Methods We searched for the studies on ADHD at China National Knowledge Infrastructure (CNKI, http://www.cnki.net) from 2011.1 to 2013.8. Results Selected 12 studies were divided into 7 case reports and 5 control studies. 7 case reports reported significant improvements with acupuncture therapy in ADHD. 5 control studies reported results that the acupuncture therapy groups were similar or better than western medicine treatment groups. Frequently used acupuncture points were EX-HN23 (7 times), GV20 ${\bullet}$ P6 ${\bullet}$ SP6 (6 times), G13 ${\bullet}$ EX-HN1 (5 times). Conclusions There have been reported many ADHD clinical studies in China and these studies can be applied to the clinical practices in Korea.

• 한국환경보건학회지
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• 제18권2호
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• pp.26-38
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• 1992

This study was carried out to investigate phytoplankton distributions and water properties at 6 sampling places in Yongsan lake during the period from Jun. 30 to Nov. 17, 1991.Results obtained were as follows: 1. At sampled sites, BOD value ranges from 1.0~14.0 mg/l except Sangmongtan where it was 14.0 mg/l on Nov. 17. 2. T-N and T-P were generally high and the former was 0.26~1.42 mg/l, the latter 0.010~0.158mg/l. 3. At all sampled places, salinity was in the range of 0.04~0.57% but especially downstream of Yongsan lake was high, 0.39~0.57% on Oct. 27. 4. The phytoplankton identification in this survey period showed Cyanophyceae is 11 genera 14 species, Bacillariophyceae 13 genera 22 species, Chlorophyceae 30 genera 55 species: total 54 genera 91 species. 5. Dominant species were Anacystis aeruginosa, Lyngbya contorta, Anabeana Sp, Melosira granulata, Melosira granulata var. angustissima, Dictyosphaerium pulchellurn and Actinastrurn hantzschii var. fiuviatile during the period from summer to fall. 6. Lyngbya contorta was seemed to adapt to the circumstance of low transparency. 7. Phytoplankton distributions were seemed to be influenced by salinity and thransparency. And so they might appear much fewer in the downstream than the upperstream in Yongsan lake.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권9호
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• pp.3449-3467
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• 2015

A broadcast operation is the fundamental transmission technique in mobile ad-hoc networks (MANETs). Because a broadcast operation can cause a broadcast storm, only selected forwarding nodes have the right to rebroadcast a broadcast message among the one-hop and two-hop neighboring nodes of a sender. This paper proposes the maximum intersection self-pruning (MISP) algorithm to minimize broadcasting redundancy. Herein, an example is given to help describe the main concept of MISP and upper bounds of forward node have been derived based on induction. A simulation conducted demonstrated that when conventional blind flooding (BF), self-pruning (SP), an optimized link state routing (OLSR) multipoint relay (MPR) set, and dominant pruning (DP), are replaced with the MISP in executing Strong duplicate address detection (DAD), the performances in terms of the energy consumption, upper bounds of the number of forward nodes, and message complexity have been improved. In addition, to evaluate the performance in reference to the link error probability, P_e, an enhancement was achieved by computing a proposed retransmission limit, S, for error recovery based on this probability. Retransmission limit control is critical for efficient energy consumption of MANET nodes operating with limited portable energy where Strong DAD reacts differently to link errors based on the operational procedures.

• 환경생물
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• 제32권4호
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• pp.286-296
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• 2014

본 연구는 울진 바다목장 해역에서 서식하는 대형저서동물의 생물다양성, 개체수 및 군집구조를 파악하기 위하여 실시되었다. 연구해역 연성기질 조하대에서 출현한 대형저서동물은 345종 $8m^{-2}$에 단위면적당($m^{-2}$) 출현 개체수는 5,797개체이었다. 분류군별 출현 종수와 개체수에 있어서는 환형동물의 다모류가 각각 154종, 44.64%와 193,320개체, 83.37%의 점유율을 나타내 가장 우점하는 생물이었다. 한편 연구해역의 주요 우점종으로 다모류의 민얼굴갯지렁이(Spiophanes bombyx)는 124,385개체 (평균 $3,110{\pm}4,434$개체 $m^{-2}$), 53.64%의 점유율을 나타내었고, 다음으로 Magelona sp.1이 16,130개체 (평균 $403{\pm}926$개체 $m^{-2}$)로 6.96%를 차지하였다. 이 밖에 이매패류의 Cadella semitorta, 다모류의 긴자락송곳갯지렁이(Lumbrineris longifolia) 및 이매패류의 겨자씨조개 (Alvenius ojianus)의 순서로 각각 6,325개체(2.73%), 5,010개체 (2.16%) 및 4,820개체 (2.08%)가 출현하였다. 한편 출현 종과 개체수의 자료를 근거로 대형저서동물의 군집구조 분석을 실시한 결과, 크게 2개의 그룹과 1개의 정점 (5월의 정점 4)으로 구분되었다. 이 가운데 그룹 1은 모든 조사시기의 정점 2, 3, 5, 6, 8 및 9가 포함되었고, 환경적으로 30~60 m 내외의 수심과 세립질 퇴적물이 우세한 정점들이라는 공통점이 있었다. 또한 본 그룹에 속하는 대형저서동물은 총 282종(평균 $66{\pm}15$종)과 7,471개체 $m^{-2}$의 밀도에 다모류의 Magelona sp.1, 긴자락송곳갯지렁이, 삼각모자갯지렁이(Scoloplos armiger), 꼬리대나무갯지렁이 (Praxillella affinis), 민숭대나무갯지렁이 (Maldane cristata)와 겨자씨조개 등이 주요한 구성종이었다. 반면 그룹 2는 모든 조사시기의 정점 1, 4, 7 및 10으로 구성되었고, 환경적으로 수심과 퇴적상은 각각 5m 내외의 조립질 퇴적물이 우세한 정점들이었다. 또한 생물적으로는 181종 (평균 $37{\pm}18$종)과 3,287개체 $m^{-2}$를 나타내었고, 주요 우점종은 이매패류의 Cadella semitorta와 다모류의 Lumbrineriopsis sp.이었다. 이상을 종합해 보면, 연구해역에 서식하는 대형저서동물 군집은 연안으로 부터의 이격정도와 그에 따른 수심과 퇴적물의 조성차이에 의해 그 구조를 달리하고 있었다.

• 한국식품과학회지
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• 제38권1호
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• pp.82-87
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• 2006

전통 청국장에서 protease 활성이 우수한 21개 균주를 ISP 배지를 이용하여 선발하였다. 선발된 21개 균주로 각각 청국장을 제조 후 관능 평가를 통해 청국장 특유의 이취와 비슷한 향을 생성하는 8균주를 선발 및 49개 탄소원의 이용성을 조사하여 Bacillus subtilis MB4와 B. amyloliquefaciens A1, A2, B1, MC1, SB2, SC1, SD1로 명명하였다. 최종적으로 우수한 균주로 선정된 MB4, A2와 SB2는 PCR sequencing(16S rDNA)에 의한 상동성을 비교하여 B. subtilis MB4, B. amyloliquefaciens A2, B. amyloliquefaciens SB2로 각각 동정되었다. B. subtilis MB4 및 B. amyloliquefaciens SB2로 제조한 청국장의 protease 활성은 179.6(Unit/mL/min)과 201.9(Unit/mL/min)로, 대조구의 97.0(U nit/mL/min)보다 우수했으며, 관능 평가는 B. subtilis MB4로 제조한 청국장이 3.6점의 평점을 얻어 전통 청국장과 가장 비슷한 향을 가진 것으로 평가 되었다. 겉보기 점도는 B. amyloliquefaciens SB2, MC1, B1, A1, SD1, A2, SC1로 제조한 청국장은 각각 0.010-0.013 Pa s (404/s)에서 0.008-0.006 Pa s(1001/s)로 감소하는 추세를 보였다. Bacillus subtilis MB4로 제조된 청국장과 대조구인 전통 청국장의 경우 각각 0.0008, 0.0006 Pa s(404/s)에서 0.0007, 0.0002 Pa s (1001/s)로 거의 변화가 없었으며, 이러한 결과는 B. subtilis MB4로 제조된 청국장과 대조구인 전통 청국장이 매우 유사한 물성을 가지는 것으로 판단된다.

• Tuberculosis and Respiratory Diseases
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• 제84권2호
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• pp.125-133
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• 2021

Background: The coronavirus disease (COVID-19) can manifest in a range of symptoms, including both asymptomatic systems which appear nearly non-existent to the patient, all the way to the development of acute respiratory distress syndrome (ARDS). Specifically, COVID-19-associated pneumonia develops into ARDS due to the rapid progression of hypoxia, and although arterial blood gas analysis can assist in halting this deterioration, the current environment provided by the COVID-19 pandemic, which has led to an overall lack of medical resources or equipment, has made it difficult to administer such tests in a widespread manner. As a result, this study was conducted in order to determine whether the levels of oxygen saturation (SpO₂) and the fraction of inhaled oxygen (FiO₂) (SF ratio) can also serve as predictors of ARDS and the patient's risk of mortality. Methods: This was a retrospective cohort study conducted from February 2020 to Mary 2020, with the study's subjects consisting of COVID-19 pneumonia patients who had reached a state of deterioration that required the use of oxygen therapy. Of the 100 COVID-19 pneumonia cases, we compared 59 pneumonia patients who required oxygen therapy, divided into ARDS and non-ARDS pneumonia patients who required oxygen, and then investigated the different factors which affected their mortality. Results: At the time of admission, the ratios of SpO₂, FiO₂, and SF for the ARDS group differed significantly from those of the non-ARDS pneumonia support group who required oxygen (p<0.001). With respect to the predicting of the occurrence of ARDS, the SF ratio on admission and the SF ratio at exacerbation had an area under the curve which measured to be around 85.7% and 88.8% (p<0.001). Multivariate Cox regression analysis identified that the SF ratio at exacerbation (hazard ratio [HR], 0.916; 95% confidence interval [CI], 0.846-0.991; p=0.029) and National Early Warning Score (NEWS) (HR, 1.277; 95% CI, 1.010-1.615; p=0.041) were significant predictors of mortality. Conclusion: The SF ratio on admission and the SF ratio at exacerbation were strong predictors of the occurrence of ARDS, and the SF ratio at exacerbation and NEWS held a significant effect on mortality.