• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.502-509
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• 2005

Sexual reproduction plays an important role in the biology and epidemiology of oomycete plant pathogens such as the heterothallic species Phytophthora infestans. Recent worldwide dispersal of A2 mating type strains of P. infestans resulted in increased virulence, gene transfer, and genetic variation, creating new challenges for disease management. To develop a genetic assay for mating type identification in P. infestans, we used the Amplified Fragment Length Polymorphism (AFLP) technique. The primer combination E+AT/M+CTA detected a fragment specific to A1 mating type (Mat-A1) of P. infestans. This fragment was cloned and sequenced, and a pair of primers (INF-1, INF-2) were designed and used to differentiate P. infestans Mat-A1 from Mat-A2 strains. The Mat A1-specific fragment was detected using Southern blot analysis of PCR products amplified with primers INF-1 and INF-2 from genomic DNA of 14 P. infestans Mat-A1 strains, but not 13 P. infestans Mat-A2 strains or 8 other isolates representing several Phytophthora spp. Southern blot analysis of genomic DNAs of P. infestans isolates revealed a 1.6 kb restriction enzyme (EcoRI, BamHI, AvaI)-fragment only in Mat-A1 strains. The A1 mating type-specific primers amplified a unique band under stringent annealing temperatures of $63^{\circ}C-64^{\circ}C$, suggesting that this PCR assay could be developed into a useful method for mating type determination of P. infestans in field material.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.181-188
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• 2006

Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing external and internal forms of these materials. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Southern type of Osterici Radix has short rhizome and many small and long roots, while northern type of it is mostly composed of rhizomes with little small reds and sometimes looks like bamboo because of the extention of the parts between joints. 2. Parenchymal cell of southern type of Osterici Radix is filled with starch grain, while that of nothern type includes less starch grain with lamination crossed on its cell wall. 3. Surface of root and rhizome of wild Osterici Radix has some marks of fibrous root raised in the form of nodule, but cultivated Osterici Radix does not have this kind of marks. 4. Notopterigii Rhizoma et Radix is mostly composed of rhizomes, and its form is similar to that of a silkworm or joints of bamboo. Parenchymal cell is filled with starch grains, and lamination is crossed on its cell wall but not salient. Conclusion : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix circulated in Korea can be easily identified because of their differences in their external and internal forms.

• Journal of Plant Biology
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• v.17 no.3
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• pp.107-112
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• 1974

Leaf temperature is complicated with the microclimate and the dry matter production in a plant community. But a daily change of leaf temperature varying by the locality in plant body or plant community is not yet clear. To resolve such a question, following experiment was designed; Helianthus tuberosus L., Glycine max L., Zea mays L., Impomoea batatas Lam., and Cucurbita moschata var. toonas Makino were planted in the different sandy loam, $2m{\times}2m-quadrat$, which has a eastern, southern, western and northern edge. In each plot 17-25 plants were planted and the distances between individuals spaced uniformly. And leaf temperature were measured by MR3-C type thermistor from 14th May through 20th August. It is seems that the upper leaf is affected by solar radiation, the lower leaf by released heat from the earth and the middle leaf by the conditions of both zones. Measuring the temperature of a leaf that is on terminal, central, left and right margins and base part, temperature of control plant in a leaf was sloped during about two hours from noon. It is noticeable as a "noon sleeping" phenomenon.henomenon.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.224-229
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• 2017

A new peanut variety 'Daan'(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2014. This was developed from the cross between 'Iksan 31' with Virginia typed short stem and 'Dakwang' with Shinpung-typed larger grain. 'Daan' which is a Shinpung plant type had 13 branches per plant and its length of main stem was 44cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 34 pods per plant, 127 g of 100-seed-weight and 75% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety showed resistant to early leaf spot and had more resistant to late leaf spot, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years 'Daan' was more productive than reference variety by 16% with 5.00 MT/ha for grain production.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.391-413
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• 1994

The northernmost type of the monogolian oak forests in the Russian Far East was studied in terma of syntaxonomy and synchorology. Hand-sorting method, computer program TWIN-SPAN, CANOCO and SYN-TAX III were engaged for the classification and data analysis. Correlation between plant communitie and environmental factors was analyzed by DCA (Detrended Correspondence Analysis) using NeCD (Net Contribution Degree) of species. Three plant communities were recognized: the Abieti holophyllae-Quercetum mongolicae, the Lespedezo-quercetum mongolicas and the Rosa ussuriensis-Quercus mongolica communit. They were included into the alliance Jeffersonio-Quercion monogolicae which is representative to the cool-Temperate forests mixed by conierous and broadleaved trees in southern Sikhote Alin. Human impact was signified as the most important factor to interpret the disjunction of plant communities of which DCA ordination yielded sharp contrasting objects (i.e. stands and species). In the amhi-Tonghae region, alaogous vegetation types such as the Jeffersonio-Quercion monogolicae of Russian Far East, the Quercion grosseserratae of Japanese Hokkaido and the Pino koraiensis-Quercion monogolicae of Koream peninusula were reviewed in terms of similarity of species composition and stratum structure. It is signified that the Jeffersonio-Quercion monogolicae was one of the continental types more similar to Korean alliance that to Japanese one.

• The Plant Pathology Journal
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• v.24 no.3
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• pp.357-360
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• 2008

Several pathotypes have been recognized in citrus bacterial canker, which causing serious damage in citrus cultivation area. To control the disease, it is important to understand the pathological diversity and reason of difference in virulence of the causal pathogen. We analyzed 124 strains of Xanthomonas causing citrus bacterial canker by southern hybridization with an internal 3.4-kb BamHI fragment from pthA gene. Assuming each band represented an intact gene, each strain of Xanthomonas was estimated to have approximately 1 to 4 copies of pthA gene. X. a. pv. citri A type had more than 3 copies of pthA gene, and the number of pthA gene in X. a. pv. citri $A^*,\;A^w$, and X. a. pv. aurantifolii B, C were different from 1 to 3 according to the strains. When the pthA gene profile was classified into 13 groups according to the number and size of hybridization bands, most of the A types belong to the 3A group, and 4A and 4B type was dominant when they had 4 bands. However, there was no general pattern of difference between the virulence and pthA gene group in this test.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.3
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• pp.67-74
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• 2012

This study was carried out to cultivate evergreen-leaved tree container seedlings having low cost of equipment and easy to control. Two species of V. odoratissimum var. awabuki and Q. glauca planting in respective underground container were showed survival rate above 90%. The Relative growth rates of height was good in non-treatment and root collar diameter was no significant difference between treatments. The T/R ratio was respectively 1.1, 1.0 in container type of F, G. and root's biomass products was more effective than others. A container type of F showed better Quailty index than others with 16.2 values. Q. glauca was higher T/R ratio in root development than shoot and the quality index was high in container type of E, D with 4.0. 3.5, respectively. On the basis of the results obtained in this study, we can concluded that the underground container type was suitable to F in V. odoratissimum var. awabuki and to D or E in Q. glauca.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.2
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• pp.77-82
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• 2006

An efficient transformation system for the production of transgenic plants has been developed for tall fescue (Festuca arundinacea Schreb.) via Agrobacterium-mediated transformation of seed-derived callus. From the point of morphogenetic capacity, three types of callus were selected. High frequency of plant regeneration was obtained by selection of type II callus, and the plant regeneration frequency was 52.6% when embryogenic callus were cultured on the regeneration medium. Supplementation of the media with 10 mg/L $AgNO_3$ and 40 mg/L cysteine enhanced frequencies of plant regeneration up to 65.3%. The highest transformation efficiency was also obtained when type II callus were inoculated with Agrobacterium. Southern blot analysis of PCR products of transgenic plants demonstrated that transgenes were successfully integrated into the genome of tall fescue. Efficient regeneration system and transformation established in this study will be useful for molecular breeding of tall fescue through genetic transformation.

• Korean Journal of Plant Taxonomy
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• v.48 no.2
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• pp.123-128
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• 2018

Earlier taxonomic studies of Korean Scutellaria reported a new record of Scutellaria hastifolia L. in Korea based on three herbarium sheets. During a reexamination of these specimens, we found that the leaf characters of these specimens differ from those in the type specimen of S. hastifolia. Based on a literature survey and confirmation of the type specimen, the specimens identified as S. hastifolia thus far were a misidentification of S. barbata D. Don. S. hastifolia is clearly different from S. barbata by single conspicuous teeth on both sides of the leaf margins and larger leaves. In addition to the distribution sites of the three specimens used in the previous study, a distribution site of the S. barbata was newly found in the southern part of Korea. In this study, we report a new distribution of S. barbata in Korea, correct a previous report of S. hastifolia, describe the morphological characters of S. barbata, and suggest a taxonomic key to Korean Scutellaria including S. barbata.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.98-98
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• 2017

Leaf senescence is the process of aging in plants. Chlorophyll degradation during leaf senescence has the important role translocating nutrients from leaves to storage organs. The functional stay-green with slow leaf yellowing and photosynthesis activity maintenance has been considered one of strategy for increasing crop productivity. Here, we have identified two QTLs on chromosome 9 and 10 for leaf senescence with chlorophyll content of RIL population derived from a cross between Hanareum 2, early leaf senescence Indica-type variety, and Unkwang, delayed leaf senescence Japonica variety. Among these QTLs, we chose qPLS1 QTL on chromosome 9 for further study. qPLS1 was found to explain 14.4% of the total phenotypic variation with 11.2 of LOD score. Through fine-mapping approach, qPLS1 QTL locus was narrowed down to about 25kb in the marker interval between In/del-4-7-9 and In/del-5-9-4. There are 3 genes existed within 25kb of qPLS1 locus: LOC_Os09g36200, LOC_Os09g36210, and LOC_Os09g36220. Among these genes, transcript level of LOC_Os09g36200 was increased during the leaf senescence stage and the expression level of LOC_Os09g36200 in Indica was higher than in Japonica. Finally, we chose LOC_Os09g36200 as candidate gene and renamed it as OsPLS1-In and OsPLS1-Jp from Indica- and Japonica-type rice, respectively. OsPLS1-In and OsPLS1-Jp overexpressing transgenic plants showed both early leaf senescence phenotype. These results indicate that OsPLS1 functions in chlorophyll degradation and the difference of expression level of OsPLS1 cause the difference of leaf senescence between Indica and Japonica in rice.